Abstract:
:To clarify the role of core protein phosphorylation in pregenomic-RNA encapsidation of human and duck hepatitis B viruses (HBV and DHBV, respectively), we have examined the phosphorylation states of different forms of intracellular HBV core protein and the phenotypic effects of mutations in the phosphorylation sites of HBV and DHBV core proteins. We show that HBV core protein is phosphorylated to similar extents in the form of protein dimers and after further assembly in pregenomic RNA-containing capsids. Individual and multiple substitutions of alanine and aspartic acid for serine in the phosphorylation sites of HBV core protein resulted in site-specific and synergistic effects on RNA encapsidation, ranging from 2-fold enhancement to more than 10-fold inhibition. Core protein variants with mutations in all phosphorylation sites exhibited dominant-negative effects on RNA encapsidation by wild-type protein. The results suggest that the presence of phosphoserine at position 162 of HBV core protein is required for pregenomic-RNA encapsidation, whereas phosphoserine at position 170 optimizes the process and serine might be preferable in position 155. Examination of the pregenomic-RNA-encapsidating capacities of DHBV core protein variants, in which four phosphorylation sites were jointly mutated to alanine or aspartic acid, suggests that phosphorylation of DHBV core protein at these sites may optimize pregenomic-RNA encapsidation but that its impact is much less profound than in the case of HBV. The possible mechanisms by which RNA encapsidation may be modulated by core protein phosphorylation are discussed in the context of the observed differences between the two viruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gazina EV,Fielding JE,Lin B,Anderson DAdoi
10.1128/jvi.74.10.4721-4728.2000subject
Has Abstractpub_date
2000-05-01 00:00:00pages
4721-8issue
10eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
杂志文章abstract::Corynebacteriophages beta and gamma are closely related but heteroimmune; hence, gamma reproduces in C7(beta). A series of gamma mutants, designated gamma-bin (beta-inhibited), has been isolated. They reproduce in only 2 to 14% of infected C7(beta) cells, and, as a result, plaque with an efficiency of 10(-4) to 10(-5)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.28-33.1978
更新日期:1978-10-01 00:00:00
abstract::In polarized T cells, HIV-1 Gag localizes to a rear-end protrusion known as the uropod in a multimerization-dependent manner. Gag-laden uropods participate in formation of virological synapses, intercellular contact structures that play a key role in cell-to-cell HIV-1 transmission. Our previous observations suggest t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00040-13
更新日期:2013-06-01 00:00:00
abstract::The adenovirus major late transcription unit (MLTU) encodes five families of mRNAs, L1 to L5, each distinguished by a unique poly(A) site. Use of the promoter-proximal L1 poly(A) site predominates during early infection, whereas poly(A) site choice shifts to the promoter-distal sites during late infection. A mini-MLTU...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1775-1783.1996
更新日期:1996-03-01 00:00:00
abstract::To assess the possible contribution of host immune responses to the exertion of Fv2-associated resistance to Friend virus (FV)-induced disease development, we inoculated C57BL/6 (B6) mice that lacked various subsets of lymphocytes with FV containing no lactate dehydrogenase-elevating virus. Fv2(r) B6 mice lacking CD4(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02506-13
更新日期:2013-12-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) has been shown to progress through a number of changes that lead to the emergence of pathogenic viral variants in macaques initially infected with a mildly cytopathic variant, SIVMneCL8. One of these late-stage isolates, SIVMne170, replicates to high levels in vivo and causes a rapi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15091-15098.2005
更新日期:2005-12-01 00:00:00
abstract::Natural killer (NK) cells serve as a crucial first-line defense against tumors and virus-infected cells. We previously showed that lysis of influenza virus (IV)-infected cells is mediated by the interaction between the NK receptor, NKp46, and the IV hemagglutinin (HA) type 1 expressed by the infected cells. This inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01815-09
更新日期:2010-04-01 00:00:00
abstract::The initial wave of swine-origin influenza A virus (pandemic H1N1/09) in the United States during the spring and summer of 2009 also resulted in an increased vigilance and sampling of seasonal influenza viruses (H1N1 and H3N2), even though they are normally characterized by very low incidence outside of the winter mon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00018-10
更新日期:2010-06-01 00:00:00
abstract::The deployment of adenovirus serotype 5 (Ad5)-based vectors is hampered by preexisting immunity. When such vectors are delivered intravenously, hepatocyte transduction is mediated by the hexon-coagulation factor X (FX) interaction. Here, we demonstrate that human sera efficiently block FX-mediated cellular binding and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01878-08
更新日期:2009-01-01 00:00:00
abstract::It has been reported that gene expression directed by the long terminal repeat of Rous sarcoma virus (RSV) is trans activated by a protein encoded in an alternate reading frame within the RSV gag gene (S. Broome and W. Gilbert, Cell 40:537-546, 1985). We have made specific mutations to test the role of the putative tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.11.4003-4008.1988
更新日期:1988-11-01 00:00:00
abstract::Epstein-Barr virus (EBV) efficiently drives proliferation of human primary B cells in vitro, a process relevant for human diseases such as infectious mononucleosis and posttransplant lymphoproliferative disease. Human B-cell proliferation is also driven by ligands of Toll-like receptors (TLRs), notably viral or bacter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01400-09
更新日期:2010-04-01 00:00:00
abstract::We analyzed a region of the capsid of canine parvovirus (CPV) which determines the ability of the virus to infect canine cells. This region is distinct from those previously shown to determine the canine host range differences between CPV and feline panleukopenia virus. It lies on a ridge of the threefold spike of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9214-9222.1997
更新日期:1997-12-01 00:00:00
abstract::Since most human immunodeficiency virus (HIV) infections are initiated following mucosal exposure to the virus, the anatomic containment or abortion of an HIV infection is likely to require vaccine-elicited cellular immune responses in those mucosal sites. Studying vaccine-elicited mucosal immune responses has been pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11484-11490.2002
更新日期:2002-11-01 00:00:00
abstract::A ribonucleoprotein particle containing about 20% ribonucleic acid (RNA), and containing little if any phospholipid or glucosamine, was recovered in high yield after treatment of Schmidt-Ruppin strain of Rous sarcoma virus and B77 virus with the nonionic detergent Nonidet P-40. This structure, which probably derives f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.5.1010-1020.1972
更新日期:1972-11-01 00:00:00
abstract::Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00601-08
更新日期:2008-07-01 00:00:00
abstract::We have previously demonstrated that two mouse cell lines that are poorly infectible by reovirus become highly susceptible upon transfection with the gene encoding the epidermal growth factor receptor (EGFR) (J. E. Strong, D. Tang, and P. W. K. Lee, Virology 197:405-411, 1993). This enhancement of infection efficiency...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.612-616.1996
更新日期:1996-01-01 00:00:00
abstract::Viral infection induces production of type I interferons (IFNs), which stimulate the expression of a variety of antiviral factors to inhibit viral replication. To establish effective infection, viruses need to develop strategies to evade the immune responses. A neurovirulent Sindbis virus strain with neuroinvasive pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01487-16
更新日期:2016-10-28 00:00:00
abstract::The nonpathogenic human adeno-associated virus (AAV) has developed a mechanism to integrate its genome into human chromosome 19 at 19q13.4 (termed AAVS1), thereby establishing latency. Here, we provide evidence that the chromosomal signals required for site-specific integration are conserved in the mouse genome proxim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8917-8921.2004
更新日期:2004-08-01 00:00:00
abstract::The vast majority of recombinant polytropic murine leukemia viruses (MuLVs) generated in mice after infection by ecotropic MuLVs can be classified into two major antigenic groups based on their reactivities to two monoclonal antibodies (MAbs) termed Hy 7 and 516. These groups very likely correspond to viruses formed b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8923-8927.1997
更新日期:1997-11-01 00:00:00
abstract::Lipoxins are host anti-inflammatory molecules that play a vital role in restoring tissue homeostasis. The efficacy of lipoxins and their analog epilipoxins in treating inflammation and its associated diseases has been well documented. Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL) are two well-known inflamm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01491-16
更新日期:2016-11-28 00:00:00
abstract::The proteins of purified avian oncoviruses were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and isoelectric focusing. Certain members of the avian leukosis-sarcoma viruses (ALSV) had group-specific antigens with altered electrophoretic properties. (i) The p27 protein of Rous-associated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.3.850-864.1977
更新日期:1977-12-01 00:00:00
abstract::TMC114, a newly designed human immunodeficiency virus type 1 (HIV-1) protease inhibitor, is extremely potent against both wild-type (wt) and multidrug-resistant (MDR) viruses in vitro as well as in vivo. Although chemically similar to amprenavir (APV), the potency of TMC114 is substantially greater. To examine the bas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.12012-12021.2004
更新日期:2004-11-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) infection of swine leads to a serious disease characterized by a delayed and defective adaptive immune response. It is hypothesized that a suboptimal innate immune response is responsible for the disease pathogenesis. In the study presented here we tested thi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01326-09
更新日期:2010-02-01 00:00:00
abstract::Viral DNA sequences were not detected in high-molecular-weight host DNA until well after the onset of viral DNA replication. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.397-399.1979
更新日期:1979-04-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infections in sub-Saharan Africa represent about 56% of global infections. Study of active-site mutations (the V82A single mutation and the V82F I84V double mutation) in the less-studied South African HIV type 1 subtype C (C-SA) protease indicated that neither mutation had a signific...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00726-08
更新日期:2008-11-01 00:00:00
abstract::Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a putative immediate-early protein which is homologous to herpes simplex virus type 1 (HSV-1) ICP27 on the basis of gene location and similarity in amino acid sequence. In transient expression assays, however, ORF4 and ICP27 exhibit different properties....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1987-1992.1994
更新日期:1994-03-01 00:00:00
abstract::The coronavirus nucleocapsid (N) protein is a structural protein that forms a ribonucleoprotein complex with genomic RNA. In addition to its structural role, it has been described as an RNA-binding protein that might be involved in coronavirus RNA synthesis. Here, we report a reverse genetic approach to elucidate the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.6620-6630.2005
更新日期:2005-06-01 00:00:00
abstract::The hepatitis E virus (HEV), a nonenveloped RNA virus, is the causative agent of hepatitis E. The mode by which HEV attaches to and enters into target cells for productive infection remains unidentified. Open reading frame 2 (ORF2) of HEV encodes its major capsid protein, pORF2, which is likely to have the determinant...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00717-09
更新日期:2009-12-01 00:00:00
abstract::The small membrane glycoprotein (GS) of equine arteritis virus (EAV) is a minor virion component but is abundantly expressed in EAV-infected cells. In this study, we have analyzed its membrane topology, folding, oligomerization, and intracellular transport. We show that GS is a class I integral membrane protein with o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3441-3448.1995
更新日期:1995-06-01 00:00:00
abstract::Marburg virus, a filovirus, causes severe hemorrhagic fever with hitherto poorly understood molecular pathogenesis. We have investigated here the vectorial transport of the surface protein GP of Marburg virus in polarized epithelial cells. To this end, we established an MDCKII cell line that was able to express GP per...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1274-1283.2001
更新日期:2001-02-01 00:00:00
abstract::In two cases of parenteral transmission of human immunodeficiency virus type 1 (HIV-1) syncitium-inducing (SI) variants, we previously observed selection for macrophagetropic variants. Although infection of macrophages is generally mediated via CCR5, we found no selection for SI variants that could use CCR5 as corecep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8848-8853.2001
更新日期:2001-09-01 00:00:00