Abstract:
:The coronavirus nucleocapsid (N) protein is a structural protein that forms a ribonucleoprotein complex with genomic RNA. In addition to its structural role, it has been described as an RNA-binding protein that might be involved in coronavirus RNA synthesis. Here, we report a reverse genetic approach to elucidate the role of N in coronavirus replication and transcription. We found that human coronavirus 229E (HCoV-229E) vector RNAs that lack the N gene were greatly impaired in their ability to replicate, whereas the transcription of subgenomic mRNA from these vectors was easily detectable. In contrast, vector RNAs encoding a functional N protein were able to carry out both replication and transcription. Furthermore, modification of the transcription signal required for the synthesis of N protein mRNAs in the HCoV-229E genome resulted in the selective replication of genomes that are able to express the N protein. This genetic evidence leads us to conclude that at least one coronavirus structural protein, the N protein, is involved in coronavirus replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Schelle B,Karl N,Ludewig B,Siddell SG,Thiel Vdoi
10.1128/JVI.79.11.6620-6630.2005subject
Has Abstractpub_date
2005-06-01 00:00:00pages
6620-30issue
11eissn
0022-538Xissn
1098-5514pii
79/11/6620journal_volume
79pub_type
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