Abstract:
:Epstein-Barr virus (EBV) infection is mediated by several viral envelope glycoproteins. We have assessed gp110's functions during the virus life cycle using a mutant that lacks BALF4 (DeltaBALF4). Exposure of various cell lines and primary cell samples of epithelial or lymphoid lineages to the DeltaBALF4 mutant failed to establish stable infections. The DeltaBALF4 virus, however, did not differ from wild-type EBV in its ability to bind and become internalized into primary B cells, in which it elicited a potent T-cell-specific immune reaction against virion constituents. These findings show that DeltaBALF4 viruses can reach the endosome-lysosome compartment and dovetail nicely with the previously identified contribution of gp110 to virus-cell fusion. Other essential steps of the virus life cycle were unaffected in the viral mutant; DNA lytic replication and viral titers were not altered in the absence of gp110, and DeltaBALF4 viruses complemented in trans transformed infected B cells with an efficiency indistinguishable from that observed with wild-type viruses. All of the steps of virus maturation could be observed in lytically induced 293/DeltaBALF4 cells. Induction of lymphoblastoid cells generated with transiently complemented DeltaBALF4 virus led to the production of rare mature virions. We therefore infer that gp110 is not required for virus maturation and egress in 293 cells or in B cells. The DeltaBALF4 virus's phenotypic traits, an inability to infect human cells coupled with potent antigenicity, potentially qualify this mutant as a live vaccine. It will provide a useful tool for the detailed study of EBV-cell interactions in a physiological context.
journal_name
J Viroljournal_title
Journal of virologyauthors
Neuhierl B,Feederle R,Adhikary D,Hub B,Geletneky K,Mautner J,Delecluse HJdoi
10.1128/JVI.01613-08subject
Has Abstractpub_date
2009-05-01 00:00:00pages
4616-23issue
9eissn
0022-538Xissn
1098-5514pii
JVI.01613-08journal_volume
83pub_type
杂志文章abstract::A detailed restriction endonuclease map for the genome of Bacillus subtilis phage SP01 is presented. Sites of cleavage for the restriction enzymes BglII, EcoRI, HaeIII, and SalI were determined. This physical map showed that SP01 DNA was 140 kilobases in length and contained a repeated sequence of 12.4 kilobases at it...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.1.156-171.1979
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doi:10.1128/JVI.70.8.5642-5645.1996
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7248-7256.2004
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.72.4.2806-2814.1998
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1250-1254.1994
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.00326-18
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.398-402.1989
更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.62.7.2265-2273.1988
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11448-11458.2003
更新日期:2003-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.777-783.1984
更新日期:1984-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.3.359-367.1973
更新日期:1973-03-01 00:00:00
abstract::The recognition of naturally occurring rhadinoviruses in macaque monkeys has spurred interest in their use as models for human infection with Kaposi sarcoma-associated herpesvirus (human herpesvirus 8). Rhesus macaques (Macaca mulatta) and pig-tailed macaques (Macaca nemestrina) were inoculated intravenously with rhad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.10320-10328.1999
更新日期:1999-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.3117-3124.1995
更新日期:1995-05-01 00:00:00
abstract::ABT-378, a new human immunodeficiency virus type 1 (HIV-1) protease inhibitor which is significantly more active than ritonavir in cell culture, is currently under investigation for the treatment of AIDS. Development of viral resistance to ABT-378 in vitro was studied by serial passage of HIV-1 (pNL4-3) in MT-4 cells....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7532-7541.1998
更新日期:1998-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.6.3451-3459.2003
更新日期:2003-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1475-1487.2002
更新日期:2002-02-01 00:00:00