Abstract:
:The U(L)17 protein (pU(L)17) of herpes simplex virus 1 (HSV-1) likely associates with the surfaces of DNA-containing capsids in a heterodimer with pU(L)25. pU(L)17 is also associated with viral light particles that lack capsid proteins, suggesting its presence in the tegument of the HSV-1 virion. To help determine how pU(L)17 becomes incorporated into virions and its functions therein, we identified pU(L)17-interacting proteins by immunoprecipitation with pU(L)17-specific IgY at 16 h postinfection, followed by mass spectrometry. Coimmunoprecipitated proteins included cellular histone proteins H2A, H3, and H4; the intermediate filament protein vimentin; the major HSV-1 capsid protein VP5; and the HSV tegument proteins VP11/12 (pU(L)46) and VP13/14 (pU(L)47). The pU(L)17-VP13/14 interaction was confirmed by coimmunoprecipitation in the presence and absence of intact capsids and by affinity copurification of pU(L)17 and VP13/14 from lysates of cells infected with a recombinant virus encoding His-tagged pU(L)17. pU(L)17 and VP13/14-HA colocalized in the nuclear replication compartment, in the cytoplasm, and at the plasma membrane between 9 and 18 h postinfection. One possible explanation of these data is that pU(L)17 links the external face of the capsid to VP13/14 and associated tegument components.
journal_name
J Viroljournal_title
Journal of virologyauthors
Scholtes LD,Yang K,Li LX,Baines JDdoi
10.1128/JVI.00277-10subject
Has Abstractpub_date
2010-08-01 00:00:00pages
7642-50issue
15eissn
0022-538Xissn
1098-5514pii
JVI.00277-10journal_volume
84pub_type
杂志文章abstract:UNLABELLED:Uracil DNA glycosylases (UNG) are highly conserved proteins that preserve DNA fidelity by catalyzing the removal of mutagenic uracils. All herpesviruses encode a viral UNG (vUNG), and yet the role of the vUNG in a pathogenic course of gammaherpesvirus infection is not known. First, we demonstrated that the v...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.2.335-339.1983
更新日期:1983-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.02201-08
更新日期:2009-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.805-812.1978
更新日期:1978-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1977-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2879-2886.1993
更新日期:1993-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.534-539.1975
更新日期:1975-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00661-06
更新日期:2006-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6553-6561.1991
更新日期:1991-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00499-08
更新日期:2008-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.1.47-52.1971
更新日期:1971-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02477-13
更新日期:2014-02-01 00:00:00
abstract::Human T-cell leukemia virus (HTLV) Tax protein has been implicated in the HTLV oncogenic process, primarily due to its pleiotropic effects on cellular genes involved in growth regulation and cell cycle control. To date, several approaches attempting to correlate Tax activation of the CREB/activating transcription fact...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2655-2662.2000
更新日期:2000-03-01 00:00:00
abstract::Data showing that an apoptotic reaction (the exit into the cytoplasm and nucleolytic internucleosomal degradation of chromosomal DNA, compaction and fragmentation of chromatin, cellular shrinkage, and cytoplasmic blebbing) developed in a subline of HeLa-S3 cells upon nonpermissive poliovirus infection with either a gu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1181-1189.1995
更新日期:1995-02-01 00:00:00
abstract::Five urea-resistant and two heat-resistant mutants of poliovirus types 1 and 2 were isolated and their structural and nonstructural polypeptides compared to those of their wild-type, parental strains in an attempt to correlate mutant phenotypes with alterations in specific capsid polypeptides. Four of the seven mutant...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.4.821-833.1974
更新日期:1974-10-01 00:00:00
abstract:UNLABELLED:Viruses that spread systemically from a peripheral site of infection cause morbidity and mortality in the human population. Innate myeloid cells, including monocytes, macrophages, monocyte-derived dendritic cells (mo-DC), and dendritic cells (DC), respond early during viral infection to control viral replica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01024-15
更新日期:2015-10-01 00:00:00
abstract::The 11th influenza A virus gene product is an 87-amino-acid protein provisionally named PB1-F2 (because it is encoded by an open reading frame overlapping the PB1 open reading frame). A significant fraction of PB1-F2 localizes to the inner mitochondrial membrane in influenza A virus-infected cells. PB1-F2 appears to e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7214-7224.2003
更新日期:2003-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.883-890.2006
更新日期:2006-01-01 00:00:00
abstract::We have used Northern blot hybridization to study the accumulation of specific cellular mRNAs in Vero cells infected with herpes simplex virus (HSV) type 1 or type 2. HSV-1 infection decreased the cytoplasmic levels of beta- and gamma-actin, beta-tubulin, and histone H3 and H4 mRNAs, though not all at the same rate. H...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.601-610.1985
更新日期:1985-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.241-247.1979
更新日期:1979-04-01 00:00:00
abstract::In 2010 and 2011, several devastating Newcastle disease (ND) outbreaks occurred in China, affecting broilers, layers, and breeders. The CK-JSX1-201005 virus was isolated from broiler breeder flocks vaccinated with the classical ND virus (NDV) vaccine program, but laying rate decreased from 80% to 30 to 40% in the clin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02314-12
更新日期:2012-11-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) is a human gammaherpesvirus with latent and lytic reactivation cycles. The mechanism by which KSHV evades the innate immune system to establish latency has not yet been precisely elucidated. Toll-like receptors (TLRs) are the first line of defense against viral ...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5224-5231.1992
更新日期:1992-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3478-3487.1996
更新日期:1996-06-01 00:00:00
abstract::RNA recombination occurs frequently during replication of tombusviruses and carmoviruses, which are related small plus-sense RNA viruses of plants. The most common recombinants generated by these viruses are either defective interfering (DI) RNAs or chimeric satellite RNAs, which are thought to be generated by templat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12033-12047.2003
更新日期:2003-11-01 00:00:00
abstract::Human herpesvirus 7 (HHV-7) is a recently isolated betaherpesvirus that is prevalent in the human population, with primary infection usually occurring in early childhood. HHV-7 is related to human herpesvirus 6 (HHV-6) in terms of both biological and, from limited prior DNA sequence analysis, genetic criteria. However...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5975-5989.1996
更新日期:1996-09-01 00:00:00
abstract::Adenovirus gene therapy is a promising tool in the clinical treatment of many genetic and acquired diseases. However, it has also caused pathogenic effects in organs such as the liver. The redox-sensitive transcription factors AP-1 and NF-kappaB have been implicated in these effects. To study the mechanisms of adenovi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.1.355-363.2002
更新日期:2002-01-01 00:00:00
abstract::The infection of a bacterium by a phage starts with attachment to a receptor molecule on the host cell surface by the phage. Since receptor-phage interactions are crucial to successful infections, they are major determinants of phage host range and, by extension, of the broader effects that phages have on bacterial co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-20
更新日期:2020-11-09 00:00:00
abstract::Hepatitis C virus (HCV) remains a significant threat to the general health of the world's population, and there is a pressing need for the development of new treatments and preventative vaccines. Here, we describe the generation of retrovirus-based pseudoparticles (HCVpp) incorporating a panel of full-length E1E2 clon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11095-11104.2005
更新日期:2005-09-01 00:00:00