Abstract:
:The 11th influenza A virus gene product is an 87-amino-acid protein provisionally named PB1-F2 (because it is encoded by an open reading frame overlapping the PB1 open reading frame). A significant fraction of PB1-F2 localizes to the inner mitochondrial membrane in influenza A virus-infected cells. PB1-F2 appears to enhance virus-induced cell death in a cell type-dependent manner. For the present communication we have identified and characterized a region near the COOH terminus of PB1-F2 that is necessary and sufficient for its inner mitochondrial membrane localization, as determined by transient expression of chimeric proteins consisting of elements of PB1-F2 genetically fused to enhanced green fluorescent protein (EGFP) in HeLa cells. Targeting of EGFP to mitochondria by this sequence resulted in the loss of the inner mitochondrial membrane potential, leading to cell death. The mitochondrial targeting sequence (MTS) is predicted to form a positively charged amphipathic alpha-helix and, as such, is similar to the MTS of the p13(II) protein of human T-cell leukemia virus type 1. We formally demonstrate the functional interchangeability of the two sequences for mitochondrial localization of PB1-F2. Mutation analysis of the putative amphipathic helix in the PB1-F2 reveals that replacement of five basic amino acids with Ala abolishes mitochondrial targeting, whereas mutation of two highly conserved Leu to Ala does not. These findings demonstrate that PB1-F2 possesses an MTS similar to other viral proteins and that this MTS, when fused to EGFP, is capable of independently compromising mitochondrial function and cellular viability.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gibbs JS,Malide D,Hornung F,Bennink JR,Yewdell JWdoi
10.1128/jvi.77.13.7214-7224.2003subject
Has Abstractpub_date
2003-07-01 00:00:00pages
7214-24issue
13eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::We report the isolation and propagation of human papillomavirus type 16, the main agent of cervical cancer, using human foreskin fragments implanted in severe combined immunodeficiency mice. The infection produced viral particles, and with each passage of the virus it caused lesions identical to intraepithelial neopla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.5256-5261.1998
更新日期:1998-06-01 00:00:00
abstract::We have previously shown that two inhibitors specific for cellular cyclin-dependent kinases (cdks), Roscovitine (Rosco) and Olomoucine (Olo), block the replication of herpes simplex virus (HSV). Based on these results, we demonstrated that HSV replication requires cellular cdks that are sensitive to these drugs (L. M....
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pub_type: 杂志文章
doi:10.1128/jvi.74.5.2107-2120.2000
更新日期:2000-03-01 00:00:00
abstract::The involvement of mouse mammary tumor virus (MTV) in the development of mammary tumors of nonviral etiology in BALB/c mice was studied by measuring the levels of MTV RNA, MTV DNA, and MTV proteins in spontaneously arising and hormonally, chemically, and/or physically induced mammary tumors of BALB/c females. The foll...
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doi:10.1128/JVI.31.1.63-72.1979
更新日期:1979-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.11.3401-3409.1987
更新日期:1987-11-01 00:00:00
abstract::Early sensing of viral components or infection-induced tissue damage is a prerequisite for the successful control of pathogenic viruses by the host innate immune system. Recent results from our laboratory show how immune cells use the DNA-sensing machinery to detect intracellular damage generated early during infectio...
journal_title:Journal of virology
pub_type: 杂志文章,评审
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abstract::A protamine-like protein named P6.9 is thought to play a role in the condensation of genomes of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) during an infection. Previous studies have shown that P6.9 is phosphorylated immediately upon synthesis and dephosphorylated upon the entry of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00438-12
更新日期:2012-11-01 00:00:00
abstract::The 55-kDa E1B protein of adenovirus, which binds to and inactivates the tumor suppressor protein p53, is not expressed in the adenoviral mutant termed ONYX-015 (i.e., dl1520). It was reported that the mutant virus due to a deletion in E1B is able to replicate only in cells deficient for wild-type p53. Accordingly, dl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9470-9478.1998
更新日期:1998-12-01 00:00:00
abstract::The complete nucleotide sequences of genomic RNA1 (9,407 nucleotides [nt]) and RNA2 (8,223 nt) of Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) were determined, revealing that SPCSV possesses the second largest identified positive-strand single-stranded RNA genome among plant vir...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.1.120-127.1985
更新日期:1985-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.4.448-449.1969
更新日期:1969-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.143-152.1979
更新日期:1979-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02502-09
更新日期:2010-04-01 00:00:00
abstract::Sapelovirus is a member of the family Picornaviridae and is emerging as an enteric porcine, simian, and avian pathogen. Here, we report the genome sequence of a novel porcine sapelovirus strain YC2011 isolated from piglets with severe diarrhea. The availability of the genome sequence is helpful to further investigatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01799-12
更新日期:2012-10-01 00:00:00
abstract::When residues 17 and 18 in nef of simian immunodeficiency virus strain SIVmac239 were changed from RQ to YE, the resultant virus was able to replicate in peripheral blood mononuclear cell cultures without prior lymphocyte activation and without the addition of exogenous interleukin-2, caused extensive lymphocyte activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.4157-4161.1996
更新日期:1996-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02052-18
更新日期:2019-03-21 00:00:00
abstract::Two regions of the hepatitis B virus (HBV) genome have been shown to display properties of a transcriptional enhancer. Enhancer 1 is active in most hepatoma lines examined as well as in some non-hepatocyte-derived cell lines. In contrast, enhancer 2 activity is strictly liver specific. In this study, we show that aden...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7452-7460.1992
更新日期:1992-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.1027-1032.1996
更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.519-530.1980
更新日期:1980-08-01 00:00:00
abstract::Primary infection of a plant with a pathogen that causes high accumulation of salicylic acid in the plant typically via a hypersensitive response confers enhanced resistance against secondary infection with a broad spectrum of pathogens, including viruses. This phenomenon is called systemic acquired resistance (SAR), ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00761-17
更新日期:2017-09-12 00:00:00
abstract::Mouse mammary tumor virus (MMTV) DNA fragments were cloned into M13 bacteriophage, and the single-stranded recombinant phage DNAs were used as strand-specific nucleic acid hybridization probes to measure synthesis of plus (genomic) and minus strands of MMTV RNA in cultured cell lines and in cell-free preparations of n...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.1.60-65.1984
更新日期:1984-04-01 00:00:00
abstract::The fundamental event in prion disease is thought to be the posttranslational conversion of the cellular prion protein (PrPC) into a pathogenic isoform (PrPSc). The occurrence of PrPC on the cell surface and PrPSc in amyloid plaques in situ or in aggregates following purification complicates the study of the molecular...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9413-9418.1998
更新日期:1998-11-01 00:00:00
abstract::The double-stranded-DNA bacteriophages employ powerful molecular motors to translocate genomic DNA into preformed capsids during the packaging step in phage assembly. Bacillus subtilis bacteriophage 29 has an oligomeric prohead RNA (pRNA) that is an essential component of its packaging motor. The crystal structure of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01370-12
更新日期:2012-11-01 00:00:00
abstract::Hepatitis C virus core protein forms the viral nucleocapsid and plays a critical role in the formation of infectious particles. In this study, we demonstrate that the highly conserved residue G33, located within domain 1 of the core protein, is important for the production of cell culture-infectious virus (HCVcc). Ala...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05452-11
更新日期:2012-01-01 00:00:00
abstract::Nucleic acids from an unidentified virus from ringed seals (Phoca hispida) were amplified using sequence-independent PCR, subcloned, and then sequenced. The full genome of a novel RNA virus was derived, identifying the first sequence-confirmed picornavirus in a marine mammal. The phylogenetic position of the tentative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01240-07
更新日期:2008-01-01 00:00:00
abstract::Lethal, amber mutations in T4 genes 46 and 47 cause incomplete degradation of host DNA, premature arrest of phage DNA synthesis, accumulation of abnormal DNA replication intermediates, and defective recombination. These phenotypes can be explained by the hypothesis that genes 46 and 47 control a DNA exonuclease, but i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.1.65-77.1981
更新日期:1981-10-01 00:00:00
abstract::African swine fever virus (ASFV) multigene family 360 and 530 (MGF360/530) genes affect viral growth in macrophage cell cultures and virulence in pigs (L. Zsak, Z. Lu, T. G. Burrage, J. G. Neilan, G. F. Kutish, D. M. Moore, and D. L. Rock, J. Virol. 75:3066-3076, 2001). The mechanism by which these novel genes affect ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1858-1864.2004
更新日期:2004-02-01 00:00:00