Abstract:
:A cloned DNA copy of simian rotavirus SA11 genomic segment 10 was used to confirm the assignment of the nonstructural glycoprotein NCVP5 to this gene. Determination of the nucleotide sequence for gene 10 indicated that NCVP5 is 175 amino acids in length and has an N-terminal hydrophobic region with the characteristics of a signal sequence for membrane translocation. Unexpectedly, this region was also the location for the only two potential glycosylation sites within the molecule, asparagine residues 8 and 18. The carbohydrates carried by NCVP5 were of the high-mannose type, Man9GlcNAc and Man8GlcNAc, with the mannose 9 species predominating; no complex oligosaccharides were present. If these asparagine residues are the sites for carbohydrate attachment, this implies that cleavage of the putative signal peptide does not occur during the maturation of this nonstructural glycoprotein.
journal_name
J Viroljournal_title
Journal of virologyauthors
Both GW,Siegman LJ,Bellamy AR,Atkinson PHdoi
10.1128/JVI.48.2.335-339.1983subject
Has Abstractpub_date
1983-11-01 00:00:00pages
335-9issue
2eissn
0022-538Xissn
1098-5514journal_volume
48pub_type
杂志文章abstract::Retroviral recombination can occur between two viral RNA molecules (intermolecular) or between two sequences within the same RNA molecule (intramolecular). The rate of retroviral intramolecular recombination is high. Previous studies showed that, after a single round of replication, 50 to 60% of retroviral recombinati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9614-9623.2002
更新日期:2002-10-01 00:00:00
abstract::Our previous observations indicated that upon infection with minute virus of mice (MVM), Ehrlich ascites cells lose a transcription elongation activity which is essential for the readthrough of the MVM attenuator. This was monitored by the ability of extracts from uninfected but not from infected cells to support read...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2741-2745.1994
更新日期:1994-04-01 00:00:00
abstract::We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and O. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyces cerevisiae plasmids ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.5157-5160.1992
更新日期:1992-08-01 00:00:00
abstract::Protein synthesis in adenovirus-infected cells is regulated during the late phase of infection. The rate of initiation is maintained by a small viral RNA, virus-associated (VA) RNAI, which prevents the phosphorylation of eukaryotic initiation factor eIF-2 by a double-stranded RNA-activated protein kinase, DAI. On the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3534-3543.1993
更新日期:1993-06-01 00:00:00
abstract::Vaccinia virus (VACV) stimulates long-term immunity against highly pathogenic orthopoxvirus infection of humans (smallpox) and mice (mousepox [ectromelia virus {ECTV}]) despite the lack of a natural host-pathogen relationship with either of these species. Previous research revealed that VACV is able to induce polyfunc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00038-12
更新日期:2012-07-01 00:00:00
abstract::During viral infection, inflammation and recovery are tightly controlled by competing proinflammatory and regulatory immune pathways. Respiratory syncytial virus (RSV) is the leading global cause of infantile bronchiolitis, which is associated with recurrent wheeze and asthma diagnosis in later life. Th2-driven diseas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01295-13
更新日期:2013-10-01 00:00:00
abstract::Recently, we demonstrated that inverted repeat sequences inserted into first-generation adenovirus (Ad) vector genomes mediate precise genomic rearrangements resulting in vector genomes devoid of all viral genes that are efficiently packaged into functional Ad capsids. As a specific application of this finding, we gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9314-9324.1999
更新日期:1999-11-01 00:00:00
abstract::Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus that causes neurological diseases in a variety of warm-blooded animal species. There is general consensus that BDV can also infect humans, being a possible zoonosis. Although the clinical consequences of human BDV infection are still controversial,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.6268-6286.2002
更新日期:2002-06-01 00:00:00
abstract::A novel circular DNA virus sequence is reported from grapevine. The corresponding genomic organization, coding potential, and conserved origin of replication are similar to those of members of the family Geminiviridae, but the genome of 3,206 nucleotides is 4% larger than the largest reported geminiviral genome and sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00943-12
更新日期:2012-07-01 00:00:00
abstract::West Nile virus (WNV) is a neurotropic flavivirus and the leading cause of mosquito-borne encephalitis in the United States. Recent studies in humans have found that dysfunctional T cell responses strongly correlate with development of severe WNV neuroinvasive disease. However, the contributions of human dendritic cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00664-19
更新日期:2019-11-13 00:00:00
abstract::The Epstein-Barr virus nuclear antigen 2 (EBNA-2) acidic domain is essential for B-lymphocyte growth transformation and can activate transcription when brought to a promoter by a sequence-specific DNA-binding domain. We now show that the EBNA-2 acidic domain has slightly less activity than the proteotypic acidic trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.585-588.1995
更新日期:1995-01-01 00:00:00
abstract::The unexpectedly low efficacy of influenza vaccine during school outbreaks of influenza B virus in the spring of 1987 in Japan was probably attributable to a poor antibody response of vaccinees to the epidemic viruses. An antigenic analysis of the causative B viruses isolated in 1987 and 1988 showed much variation in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2860-2865.1990
更新日期:1990-06-01 00:00:00
abstract::An in vitro system for production of hepatitis B virus (HBV) was established by infection of human hepatoma (HepG2) cells. HBV particles obtained from the serum of a chronic hepatitis B surface antigen (subtype ad) carrier were used to inoculate HepG2 cells. HBV envelope and core proteins were synthesized de novo by t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3025-3032.1990
更新日期:1990-06-01 00:00:00
abstract::Higher-order RNA structures in the 3' untranslated region (3'UTR) of enteroviruses are thought to play a pivotal role in viral negative-strand RNA synthesis. The structure of the 3'UTR was predicted by thermodynamic calculations using the STAR (structural analysis of RNA) computer program and experimentally verified u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.686-696.1997
更新日期:1997-01-01 00:00:00
abstract::The ecotropic Cas-Br-E murine leukemia virus (MuLV) and its molecularly cloned derivative pBR-NE-8 MuLV are capable of inducing hind-limb paralysis and leukemia after inoculation into susceptible mice. T1 oligonucleotide fingerprinting, molecular hybridization, and restriction enzyme analysis previously showed that th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.910-919.1986
更新日期:1986-12-01 00:00:00
abstract::The genome of the Snyder-Theilen strain of feline sarcoma virus (ST-FeSV) is a 4.3-kilobase-pair (kbp) RNA molecule that contains a 1.5-kbp cellular insertion (fes gene) flanked by feline leukemia virus sequences at its 5' end (1.6 kbp) and 3' end (1.2 kbp) (Sherr et al., J. Virol. 34:200-212, 1980). DNA transfection ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.518-523.1981
更新日期:1981-01-01 00:00:00
abstract::A method to study the function of individual African swine fever virus (ASFV) gene products utilizing the Escherichia coli lac repressor-operator system has been developed. Recombinant viruses containing both the lacI gene encoding the lac repressor and a strong virus late promoter modified by the insertion of one or ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3185-3195.1998
更新日期:1998-04-01 00:00:00
abstract::The temperature-sensitive defects of virus mutants isolated from L cells persistently infected with Newcastle disease virus (NDV) were analyzed. Genetic grouping of the mutants by complementation tests was attempted by using several different methods, including yield analysis, RNA synthesis, and heterozygote formation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.472-480.1973
更新日期:1973-09-01 00:00:00
abstract::A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Molon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.239-248.1981
更新日期:1981-04-01 00:00:00
abstract::We provide here new insights into rotavirus (RRV) pathogenicity by showing that RRV infection promotes structural and functional injuries localized at the tight junctions (TJ) in the cell-cell junctional complex of cultured polarized human intestinal Caco-2 cells forming monolayers. RRV infection resulted in a progres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4645-4651.2000
更新日期:2000-05-01 00:00:00
abstract::Host-virus interactions control disease progression in human immunodeficiency virus-infected human beings and in nonhuman primates infected with simian or simian/human immunodeficiency viruses (SHIV). These interactions evolve rapidly during acute infection and are key to the mechanisms of viral persistence and AIDS. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.10236-10244.1999
更新日期:1999-12-01 00:00:00
abstract::Molluscs, comprising one of the most successful phyla, lack clear evidence of adaptive immunity and yet thrive in the oceans, which are rich in viruses. There are thought to be nearly 120,000 species of Mollusca, most living in marine habitats. Despite the extraordinary abundance of viruses in oceans, molluscs often h...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00287-15
更新日期:2015-08-01 00:00:00
abstract::In adenovirus-infected cells, the epidermal growth factor receptor (EGF-R) is internalized from the cell surface via endosomes and is degraded, and the E3 10,400-dalton protein (10.4K protein) is required for this effect (C. R. Carlin, A. E. Tollefson, H. A. Brady, B. L. Hoffman, and W. S. M. Wold, Cell 57:135-144, 19...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3095-3105.1991
更新日期:1991-06-01 00:00:00
abstract::Accumulated evidence demonstrates that Japanese encephalitis virus (JEV) infection triggers endoplasmic reticulum (ER) stress and neuron apoptosis. ER stress sensor protein kinase R-like endoplasmic reticulum kinase (PERK) has been reported to induce apoptosis under acute or prolonged ER stress. However, the precise r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00887-19
更新日期:2019-08-13 00:00:00
abstract::The novel low-pathogenic avian influenza A H7N9 viruses (LPAI H7N9 viruses) have been a threat to public health since their emergence in 2013 because of the high rates of mortality and morbidity that they cause. Recently, highly pathogenic variants of these avian influenza A H7N9 viruses (HPAI H7N9 viruses) have emerg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01277-17
更新日期:2017-11-14 00:00:00
abstract::Rolling circle-type molecules were found in polyoma virus-infected cells after inhibition of DNA synthesis with 2'-deoxy-2'-azidocytidine. The circular DNA molecules were always relaxed and of polyoma length. Most of the attached tails were less than two times the length of the polyoma genome, but tails with a length ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.1.136-142.1978
更新日期:1978-04-01 00:00:00
abstract::Induction of the resident prophage did not permit restricted phage T1 to replicate freely in P1-lysogenic hosts. A few induced cells did become infectible. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.4.448-449.1969
更新日期:1969-04-01 00:00:00
abstract:UNLABELLED:Despite the nutritional and health benefits of breast milk, breast milk can serve as a vector for mother-to-child HIV transmission. Most HIV-infected infants acquire HIV through breastfeeding. Paradoxically, most infants breastfed by HIV-positive women do not become infected. This is potentially attributed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01702-15
更新日期:2015-11-01 00:00:00
abstract::The herpes simplex virus (HSV) heterodimer gE/gI and another membrane protein, US9, which has neuron-specific effects, promote the anterograde transport of virus particles in neuronal axons. Deletion of both HSV gE and US9 blocks the assembly of enveloped particles in the neuronal cytoplasm, which explains why HSV vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01113-20
更新日期:2020-11-09 00:00:00
abstract::Passage of foot-and-mouth disease virus (FMDV) in cell culture in the presence of the mutagenic base analog 5-fluorouracil or 5-azacytidine resulted in decreases of infectivity and occasional extinction of the virus. Low viral loads and low viral fitness enhanced the frequency of extinction events; this finding was sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8316-8323.2000
更新日期:2000-09-01 00:00:00