Abstract:
:We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and O. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyces cerevisiae plasmids that separately express the GAL4 DNA-binding and GAL4 activation domains fused to the human immunodeficiency virus type 1 (HIV-1) gag polyprotein, Pr55gag. The coexpression of these two hybrid proteins in S. cerevisiae results in the association of the GAL4 domains and the potent activation of an integrated GAL4-responsive lacZ indicator gene. Similar results were obtained with plasmids encoding GAL4-Moloney murine leukemia virus (M-MuLV) gag polyprotein hybrid proteins. In contrast, the heterologous GAL4-HIV-1 gag and GAL4-M-MuLV gag fusion proteins were unable to interact with each other to induce lacZ expression. The results suggest that this yeast system provides a rapid and specific assay for the interactions of retroviral gag proteins that occur during virion assembly.
journal_name
J Viroljournal_title
Journal of virologyauthors
Luban J,Alin KB,Bossolt KL,Humaran T,Goff SPdoi
10.1128/JVI.66.8.5157-5160.1992subject
Has Abstractpub_date
1992-08-01 00:00:00pages
5157-60issue
8eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::Autophagy is now known to be an essential component of host innate and adaptive immunity. Several herpesviruses have developed various strategies to evade this antiviral host defense. Herpes simplex virus 1 (HSV-1) blocks autophagy in fibroblasts and in neurons, and the ICP34.5 protein is important for the resistance ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01158-12
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abstract:UNLABELLED:Influenza B virus causes annual epidemics and, along with influenza A virus, accounts for substantial disease and economic burden throughout the world. Influenza B virus infects only humans and some marine mammals and is not responsible for pandemics, possibly due to a very low frequency of reassortment and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01576-15
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abstract::Protective immunity to rotavirus (RV) is primarily mediated by antibodies produced by RV-specific memory B cells (RV-mBc). Of note, most of these cells express IgM, but the function of this subset is poorly understood. Here, using limiting dilution assays of highly sort-purified human IgM(+) mBc, we found that 62% and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01466-12
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.3.810-819.1976
更新日期:1976-09-01 00:00:00
abstract::The vaccinia virus F10 protein is one of two virally encoded protein kinases. A phenotypic analysis of infections involving a tetracycline-inducible recombinant (vDeltaiF10) indicated that F10 is involved in the early stages of virion morphogenesis, as previously reported for the mutants ts28 and ts15. The proteins en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2171-2190.2005
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abstract::The Hsp70 chaperone plays a central role in multiple processes within cells, including protein translation, folding, intracellular trafficking, and degradation. This protein is implicated in the replication of numerous viruses. We have shown that rabies virus infection induced the cellular expression of Hsp70, which a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06501-11
更新日期:2012-05-01 00:00:00
abstract::The DNA molecules from T7 bacteriophage and a recently obtained mutant form of T4D were studied. The DNA of this T4 mutant contains cytosine in place of all of the glucosylated hydroxymethylcytosines normally present in T4. Molecular weights were measured with an electron microscope technique, and sedimentation coeffi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.438-448.1980
更新日期:1980-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01708-06
更新日期:2007-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3129-3140.2001
更新日期:2001-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.4.812-819.1975
更新日期:1975-04-01 00:00:00
abstract::A retrovirus vector has been developed that selects for instances in which the provirus integrates in close proximity to cellular promoters. Coding sequences for a selectable marker (histidinol dehydrogenase) were inserted into the 3' long terminal repeat (LTR) of an enhancerless Molony murine leukemia virus. Although...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3227-3233.1989
更新日期:1989-08-01 00:00:00
abstract::Herpesvirus proteins pUL34 and pUL31 form a complex at the inner nuclear membrane (INM) which is necessary for efficient nuclear egress. Pseudorabies virus (PrV) pUL34 is a type II membrane protein of 262 amino acids (aa). The transmembrane region (TM) is predicted to be located between aa 245 and 261, leaving only on...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05484-11
更新日期:2012-02-01 00:00:00
abstract::The fundamental question of whether a primed immune system is capable of preventing latent gammaherpesvirus infection remains unanswered. Recent studies showing that vaccination can reduce acute replication and short-term latency but cannot alter long-term latency further call into question the possibility of achievin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2522-2529.2003
更新日期:2003-02-01 00:00:00
abstract::The human cytomegalovirus (HCMV) tegument protein pp71, encoded by gene UL82, stimulates viral immediate-early (IE) transcription. pp71 interacts with the cellular protein hDaxx at nuclear domain 10 (ND10) sites, resulting in the reversal of hDaxx-mediated repression of viral transcription. We demonstrate that pp71 di...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01215-08
更新日期:2008-12-01 00:00:00
abstract::Treatment of Friend erythroleukemia cells (FLC) with gamma interferon (IFN-gamma) in the presence of anti-IFN-beta antibodies reduces the effectiveness of the antiviral state and the induction of 2'-5'-oligoadenylate synthetase activity, indicating that the antiviral activity of IFN-gamma in FLC is in part mediated by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4130-4136.1991
更新日期:1991-08-01 00:00:00
abstract::We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.4021-4024.1990
更新日期:1990-08-01 00:00:00
abstract::Cell cycle progression during cytomegalovirus infection was investigated by fluorescence-activated cell sorter (FACS) analysis of the DNA content in growth-arrested as well as serum-stimulated human fibroblasts. Virus-infected cells maintained in either low (0.2%) or high (10%) serum failed to progress into S phase an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1629-1634.1997
更新日期:1997-02-01 00:00:00
abstract::Borna disease virus (BDV) is an enveloped virus with a genome organization characteristic of Mononegavirales. However, based on its unique features, BDV is considered the prototypic member of a new virus family, Bornaviridae, within the order Mononegavirales. We have described the establishment of a reverse genetics s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6544-6550.2005
更新日期:2005-05-01 00:00:00
abstract::The molecular mechanism by which retroviral Gag proteins are directed to the plasma membrane for the formation of particles (budding) is unknown, but it is widely believed that the MA domain, located at the amino terminus, plays a critical role. Consistent with this idea, we found that small deletions in this segment ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3804-3812.1991
更新日期:1991-07-01 00:00:00
abstract::Murine cytomegalovirus propagated in mouse embryo fibroblasts was purified by the following procedures. (i) Extracellular virus was concentrated by centrifugation at 100,000 x g for 90 min. (ii) The concentrated virus was passed through a Bio-Rad Bio-Gel A-15m column to eliminate contaminating materials smaller than 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.906-915.1976
更新日期:1976-03-01 00:00:00
abstract:UNLABELLED:Molluscum contagiosum virus (MOCV), the only circulating human-specific poxvirus, has a worldwide distribution and causes benign skin lesions that may persist for months in young children and severe infections in immunosuppressed adults. Studies of MOCV are restricted by the lack of an efficient animal model...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02911-15
更新日期:2016-04-14 00:00:00
abstract::Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that has a worldwide distribution in the population of cattle. Many factors make human contamination by BoHV-4 likely to occur. In this study, we performed in vitro experiments to assess the risk and the consequences of human infection by BoHV-4. First, by using a re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2336-2347.2004
更新日期:2004-03-01 00:00:00
abstract::Hepatitis delta virus (HDV) contains two types of hepatitis delta antigens (HDAg) in the virion. The small form (S-HDAg) is required for HDV RNA replication, whereas the large form (L-HDAg) potently inhibits it by a dominant-negative inhibitory mechanism. The sequential appearance of these two forms in the infected ce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.16.7375-7380.2000
更新日期:2000-08-01 00:00:00
abstract::In a previous study (S. Zheng, G. N. Woode, D. R. Melendy, and R. F. Ramig, J. Clin. Microbiol. 27:1939-1945, 1989), it was predicted that the VP7 serotype 6 bovine rotavirus strains NCDV and B641 do not share antigenically similar VP4s. In this study, gene 4 and the VP7 gene of B641 were sequenced, and the amino acid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5535-5538.1991
更新日期:1991-10-01 00:00:00
abstract::pp60v-src is a nonreceptor protein tyrosine kinase that can transform both chicken and rodent fibroblasts. The src homology 2 (SH2) domain of this protein serves a critical role in the regulation of protein tyrosine kinase activity. The host range proteins pp60v-src-L, which contains a deletion of a highly conserved r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7267-7274.1994
更新日期:1994-11-01 00:00:00
abstract::Antigens derived from host cells are detectable in the envelope of human immunodeficiency virus type 1 (HIV-1) and result in a distinctive viral phenotype reflecting that of the host cell. An immunomagnetic capture assay targeting discriminatory host proteins was developed to differentiate between HIV-1 derived from m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Cytomegaloviruses (CMVs) are members of the Betaherpesvirinae subfamily of the Herpesviridae, and their properties of latency, large DNA size, gene redundancy, and ability to be cloned as bacterial artificial chromosomes (BACs) suggest their utility as vaccine vectors. While the K181 strain of murine CMV (MCMV) is wid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2998-3008.2005
更新日期:2005-03-01 00:00:00
abstract::Assembly of hepatitis delta virus (HDV) in infected human hepatocytes involves association of the 1,679- nucleotide single-stranded genomic RNA (deltaRNA) with multiple copies of both small and large forms of the delta protein (deltaAg) to form a ribonucleoprotein particle which in turn interacts with envelope protein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3709-3719.2002
更新日期:2002-04-01 00:00:00
abstract::Five hepatoma cell lines, including CZHC/8571, PLC/PRF/5, Hep3B, HepG2, and HUH7, were inoculated with three diverse isolates of human immunodeficiency virus type 1 (HIV-1). Productive infection was noted in all hepatoma cell lines, and expression of viral p24 antigen lasted for over 3 months, but its level decreased ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2553-2559.1990
更新日期:1990-06-01 00:00:00
abstract::The recent yellow fever virus (YFV) epidemic in Brazil in 2017 and Zika virus (ZIKV) epidemic in 2015 serve to remind us of the importance of flaviviruses as emerging human pathogens. With the current global flavivirus threat, there is an urgent need for antivirals and vaccines to curb the spread of these viruses. How...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00151-19
更新日期:2019-06-28 00:00:00