Abstract:
UNLABELLED:Viruses that spread systemically from a peripheral site of infection cause morbidity and mortality in the human population. Innate myeloid cells, including monocytes, macrophages, monocyte-derived dendritic cells (mo-DC), and dendritic cells (DC), respond early during viral infection to control viral replication, reducing virus spread from the peripheral site. Ectromelia virus (ECTV), an orthopoxvirus that naturally infects the mouse, spreads systemically from the peripheral site of infection and results in death of susceptible mice. While phagocytic cells have a requisite role in the response to ECTV, the requirement for individual myeloid cell populations during acute immune responses to peripheral viral infection is unclear. In this study, a variety of myeloid-specific depletion methods were used to dissect the roles of individual myeloid cell subsets in the survival of ECTV infection. We showed that DC are the primary producers of type I interferons (T1-IFN), requisite cytokines for survival, following ECTV infection. DC, but not macrophages, monocytes, or granulocytes, were required for control of the virus and survival of mice following ECTV infection. Depletion of either plasmacytoid DC (pDC) alone or the lymphoid-resident DC subset (CD8α(+) DC) alone did not confer lethal susceptibility to ECTV. However, the function of at least one of the pDC or CD8α(+) DC subsets is required for survival of ECTV infection, as mice depleted of both populations were susceptible to ECTV challenge. The presence of at least one of these DC subsets is sufficient for cytokine production that reduces ECTV replication and virus spread, facilitating survival following infection. IMPORTANCE:Prior to the eradication of variola virus, the orthopoxvirus that causes smallpox, one-third of infected people succumbed to the disease. Following successful eradication of smallpox, vaccination rates with the smallpox vaccine have significantly dropped. There is now an increasing incidence of zoonotic orthopoxvirus infections for which there are no effective treatments. Moreover, the safety of the smallpox vaccine is of great concern, as complications may arise, resulting in morbidity. Like many viruses that cause significant human diseases, orthopoxviruses spread from a peripheral site of infection to become systemic. This study elucidates the early requirement for innate immune cells in controlling a peripheral infection with ECTV, the causative agent of mousepox. We report that there is redundancy in the function of two innate immune cell subsets in controlling virus spread early during infection. The viral control mediated by these cell subsets presents a potential target for therapies and rational vaccine design.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kaminsky LW,Sei JJ,Parekh NJ,Davies ML,Reider IE,Krouse TE,Norbury CCdoi
10.1128/JVI.01024-15subject
Has Abstractpub_date
2015-10-01 00:00:00pages
9974-85issue
19eissn
0022-538Xissn
1098-5514pii
JVI.01024-15journal_volume
89pub_type
杂志文章abstract::The features of herpes simplex virus 1 (HSV-1) strain 129 (H129), including natural neurotropism and anterograde transneuronal trafficking, make it a potential tool for anterograde neural circuitry tracing. Recently anterograde polysynaptic and monosynaptic tracers were developed from H129 and have been applied for th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01957-19
更新日期:2020-03-31 00:00:00
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doi:10.1128/JVI.16.1.27-33.1975
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2133-2139.1995
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pub_type: 杂志文章
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更新日期:2001-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00342-11
更新日期:2011-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.56.2.475-481.1985
更新日期:1985-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00373-06
更新日期:2006-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.17.8348-8352.2001
更新日期:2001-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1327-1332.2005
更新日期:2005-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01794-15
更新日期:2015-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6387-6394.1993
更新日期:1993-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.2.263-272.1972
更新日期:1972-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.78-87.1982
更新日期:1982-01-01 00:00:00
abstract:UNLABELLED:The novel H7N9 avian influenza virus (AIV) was demonstrated to cause severe human respiratory infections in China. Here, we examined poultry specimens from live bird markets linked to human H7N9 infection in Hangzhou, China. Metagenomic sequencing revealed mixed subtypes (H5, H7, H9, N1, N2, and N9). Subsequ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00653-15
更新日期:2015-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.248-255.1981
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
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abstract::A well-characterized mechanism by which anti-HLA class I monoclonal antibodies (MAb) inhibit human immunodeficiency virus type 1 (HIV-1) propagation in in vitro cell cultures is the neutralization of the virus through interactions with HLA molecules associated with the virion envelope. Yet, the possibility that anothe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5213-5220.1996
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3484-3497.1997
更新日期:1997-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.3.503-509.1972
更新日期:1972-03-01 00:00:00
abstract::Primary telencephalic cultures derived from neonatal Wistar Furth rats were able to support the growth of coronavirus JHM if a viable neuronal population was maintained. This occurred under serum-free defined, but not serum-supplemented, growth conditions. The importance of neurons in establishing infections in mixed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.5013-5028.1991
更新日期:1991-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3605-3614.2002
更新日期:2002-04-01 00:00:00
abstract::A virus recovered from the saliva of a child with chronic active Epstein-Barr virus (EBV) infection for 8 years was shown to induce EBV early antigen (EBV-EA) in Raji cells and to be expressed into EBV-EA in fresh EBV-negative peripheral blood leukocytes. However, it did not replicate its DNA. Oropharyngeal epithelial...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3306-3309.1987
更新日期:1987-10-01 00:00:00
abstract::The hepatitis B virus X protein acts as a transcriptional transactivator in vitro. To elucidate possible biological effects of X protein on liver cells in vivo, we generated four lines of transgenic mice carrying the X gene open reading frame under the control of the human alpha-1-antitrypsin regulatory region. The pl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5939-5947.1990
更新日期:1990-12-01 00:00:00
abstract::We have studied initiation and regulation of early transcription of simian virus (SV40) DNA in vitro by eucaryotic RNA polymerase II, using both a crude HeLa cell extract and a partially purified calf thymus polymerase supplemented with a HeLa cell S100 fraction. Analysis of initiation sites by primer-directed cDNA sy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.449-461.1982
更新日期:1982-02-01 00:00:00
abstract::Human cytomegalovirus (HCMV) glycoprotein US2 increases the proteasome-mediated degradation of major histocompatibility complex (MHC) class I heavy chain (HC), class II DR-alpha and DM-alpha proteins, and HFE, a nonclassical MHC protein. US2-initiated degradation of MHC proteins apparently involves the recruitment of ...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
abstract:UNLABELLED:Human metapneumovirus (HMPV), a recently discovered paramyxovirus, infects nearly 100% of the world population and causes severe respiratory disease in infants, the elderly, and immunocompromised patients. We previously showed that HMPV binds heparan sulfate proteoglycans (HSPGs) and that HMPV binding requir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01362-16
更新日期:2016-09-29 00:00:00
abstract::Human cytomegalovirus (HCMV) major immediate-early protein IE1 is an abundant 72-kDa nuclear phosphoprotein that is thought to play an important role in efficient triggering of the lytic cycle, especially at low multiplicity of infection. The best-known properties of IE1 at present are its transient targeting to punct...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.22.10683-10695.2001
更新日期:2001-11-01 00:00:00