Significant protection against high-dose simian immunodeficiency virus challenge conferred by a new prime-boost vaccine regimen.

abstract：:There are two types of infectious vaccinia virus particles: intracellular naked virions and extracellular enveloped virions (EEV). To determine the biological role of the enveloped form of vaccinia virus, we produced and characterized a mutant that is defective in EEV formation. The strategy involved replacement by ho...

journal_title：Journal of virology

authors： Blasco R,Moss B

更新日期：1991-11-01 00:00:00

abstract：:Rhabdoviruses such as rabies virus (RV) encode only five multifunctional proteins accomplishing viral gene expression and virus formation. The viral phosphoprotein, P, is a structural component of the viral ribonucleoprotein (RNP) complex and an essential cofactor for the viral RNA-dependent RNA polymerase. We show he...

journal_title：Journal of virology

authors： Finke S,Brzózka K,Conzelmann KK

更新日期：2004-11-01 00:00:00

abstract：:During the S phase of the eukaryotic cell cycle and in virus-infected cells, DNA replication takes place at discrete sites in the nucleus, although it is not clear how the proteins involved in the replicative process are directed to these sites. Nuclear factor I is a cellular, sequence-specific DNA-binding protein uti...

journal_title：Journal of virology

authors： Bosher J,Dawson A,Hay RT

更新日期：1992-05-01 00:00:00

abstract：:The 5'-untranslated regions (5' UTRs) of picornavirus genomes contain an internal ribosomal entry site (IRES) that promotes the end-independent initiation of translation. Picornavirus IRESs are classified into four structurally distinct groups, each with different initiation factor requirements. Here, we identify a fi...

journal_title：Journal of virology

authors： Sweeney TR,Dhote V,Yu Y,Hellen CU

更新日期：2012-02-01 00:00:00

abstract：:The roles of intracellular naked (INV), cell-associated enveloped (CEV), and extracellular enveloped (EEV) forms of vaccinia virus in cell-to-cell and longer-range spread were investigated by using two closely related strains of vaccinia virus, WR and IHD-J. We confirmed previous results that WR and IHD-J produced sim...

journal_title：Journal of virology

authors： Blasco R,Moss B

更新日期：1992-07-01 00:00:00

abstract：:The fusogenic properties of the uncleaved spike (S) protein of murine coronavirus JHMV variant cl-2 were studied by expressing the S protein with a deleted putative cleavage site. The amino acid sequence of the putative cleavage site, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of ...

journal_title：Journal of virology

authors： Taguchi F

更新日期：1993-03-01 00:00:00

abstract：:On the basis of the conservation of neuraminidase (N) active-site residues in influenza virus N and paramyxovirus hemagglutinin-neuraminidase (HN), it has been suggested that the three-dimensional (3D) structures of the globular heads of the two proteins are broadly similar. In this study, details of this structural s...

journal_title：Journal of virology

authors： Langedijk JP,Daus FJ,van Oirschot JT

更新日期：1997-08-01 00:00:00

abstract：:Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by tra...

journal_title：Journal of virology

authors： Gilbert J,Ou W,Silver J,Benjamin T

更新日期：2006-11-01 00:00:00

abstract：:Paramyxoviral RNAs are synthesized by a viral RNA-dependent RNA polymerase (RdRp) consisting of the large (L) protein and its cofactor phosphoprotein (P protein). The L protein is a multifunctional protein that catalyzes RNA synthesis, mRNA capping, and mRNA polyadenylation. Growing evidence shows that the stability o...

journal_title：Journal of virology

authors： Katoh H,Kubota T,Nakatsu Y,Tahara M,Kidokoro M,Takeda M

更新日期：2017-02-28 00:00:00

abstract：:This paper is concerned with the physiological role(s) of T4 phage-coded dihydrofolate reductase, which functions both in DNA precursor metabolism and as a virion protein. (i) We have detected enzyme activity in noninfectious particles produced under restrictive conditions by gene 11 mutants. This supports the conclus...

journal_title：Journal of virology

authors： Mosher RA,DiRenzo AB,Mathews CK

更新日期：1977-09-01 00:00:00

abstract：:Kilham rat virus (KRV) was grown in a rat nephroma cell line and was purified by two isopycnic centrifugations in cesium chloride. The virus contains single-stranded deoxyribonucleic acid (DNA) with a molecular weight of approximately 1.6 x 10(6). The DNA was extracted from the virion by both phenol extraction and by ...

journal_title：Journal of virology

authors： Salzman LA,White WL,Kakefuda T

更新日期：1971-06-01 00:00:00

abstract：:The genetic evolution of human immunodeficiency virus type 1 (HIV-1) in the brain is distinct from that in lymphoid tissues, indicating tissue-specific compartmentalization of the virus. Few primary HIV-1 envelope glycoproteins (Envs) from uncultured brain tissues have been biologically well characterized. In this stu...

journal_title：Journal of virology

authors： Ohagen A,Devitt A,Kunstman KJ,Gorry PR,Rose PP,Korber B,Taylor J,Levy R,Murphy RL,Wolinsky SM,Gabuzda D

更新日期：2003-11-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) tat protein functions at a much lower level in rodent cells than in human cells. This species-specific difference in trans activation appears to be due to the lack of a functional homolog of a human cofactor for tat in rodent cells. Using HIV-1 long terminal repeat-drive...

journal_title：Journal of virology

authors： Newstein M,Stanbridge EJ,Casey G,Shank PR

更新日期：1990-09-01 00:00:00

abstract：:BCX 2798 (4-azido-5-isobutyrylamino-2,3-didehydro-2,3,4,5-tetradeoxy-d-glycero-d-galacto-2-nonulopyranosic acid) effectively inhibited the activities of the hemagglutinin-neuraminidase (HN) of human parainfluenza viruses (hPIV) in vitro and protected mice from lethal infection with a recombinant Sendai virus whose HN ...

journal_title：Journal of virology

authors： Alymova IV,Taylor G,Mishin VP,Watanabe M,Murti KG,Boyd K,Chand P,Babu YS,Portner A

更新日期：2008-09-01 00:00:00

abstract：:To identify essential host genes affecting replication of Tomato bushy stunt virus (TBSV), a small model plant virus, we screened 800 yeast genes present in the yeast Tet promoters Hughes Collection. In total, we have identified 30 new host genes whose down-regulation either increased or decreased the accumulation of ...

journal_title：Journal of virology

authors： Jiang Y,Serviene E,Gal J,Panavas T,Nagy PD

更新日期：2006-08-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) has evolved multiple strategies for suppression of the antiviral response of the infected cell. DNA array technology has revealed that HCMV clearly regulates host gene expression during the course of a productive infection by enhancing, sustaining, or suppressing steady-state levels of cel...

journal_title：Journal of virology

authors： Gealy C,Denson M,Humphreys C,McSharry B,Wilkinson G,Caswell R

更新日期：2005-01-01 00:00:00

abstract：:The genome of influenza A virus consists of eight single-strand negative-sense RNA segments, each comprised of a coding region and a noncoding region. The noncoding region of the NS segment is thought to provide the signal for packaging; however, we recently showed that the coding regions located at both ends of the h...

journal_title：Journal of virology

authors： Fujii K,Fujii Y,Noda T,Muramoto Y,Watanabe T,Takada A,Goto H,Horimoto T,Kawaoka Y

更新日期：2005-03-01 00:00:00

abstract：UNLABELLED:The safety and efficacy of the live-attenuated Japanese encephalitis virus (JEV) SA14-14-2 vaccine are attributed to mutations that accumulated in the viral genome during its derivation. However, little is known about the contribution that is made by most of these mutations to virulence attenuation and vacci...

journal_title：Journal of virology

authors： Gromowski GD,Firestone CY,Whitehead SS

更新日期：2015-06-01 00:00:00

abstract：:Given the current difficulties generating vaccine-induced neutralizing antibodies to human immunodeficiency virus (HIV), the focus of the vaccine community has shifted toward creating cytotoxic-T-lymphocyte (CTL)-based vaccines. Recent reports of CTL-based vaccine trials in macaques challenged with simian/human immuno...

journal_title：Journal of virology

authors： Vogel TU,Reynolds MR,Fuller DH,Vielhuber K,Shipley T,Fuller JT,Kunstman KJ,Sutter G,Marthas ML,Erfle V,Wolinsky SM,Wang C,Allison DB,Rud EW,Wilson N,Montefiori D,Altman JD,Watkins DI

更新日期：2003-12-01 00:00:00

abstract：:Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detai...

journal_title：Journal of virology

authors： Bagewadi B,Chen S,Lal SK,Choudhury NR,Mukherjee SK

更新日期：2004-11-01 00:00:00

abstract：:It was recently shown that the 7a protein of severe acute respiratory syndrome coronavirus induces biochemical changes associated with apoptosis. In this study, the mechanism by which the 7a protein induces apoptosis was examined. The 7a protein was tested for the ability to inhibit cellular gene expression because se...

journal_title：Journal of virology

authors： Kopecky-Bromberg SA,Martinez-Sobrido L,Palese P

更新日期：2006-01-01 00:00:00

abstract：:Live attenuated RNA viruses make highly efficient vaccines. Among them, measles virus (MV) vaccine has been given to a very large number of children and has been shown to be highly efficacious and safe. Therefore, this vaccine might be a very promising vector to immunize children against both measles and other infecti...

journal_title：Journal of virology

authors： Combredet C,Labrousse V,Mollet L,Lorin C,Delebecque F,Hurtrel B,McClure H,Feinberg MB,Brahic M,Tangy F

更新日期：2003-11-01 00:00:00

abstract：:The outer layer of the bacteriophage phi 6 nucleocapsid (NC) was removed by EDTA and reassociated with the core in the presence of Ca2+ or Mg2+. The core was relatively inaccessible to trypsin digestion, was composed of protein P1, and was in the dodecahedral framework reported previously. (H.T. Steely, Jr., and D. La...

journal_title：Journal of virology

authors： Ktistakis NT,Lang D

更新日期：1987-08-01 00:00:00

abstract：:The relationship between immunosenescence and the host response to virus infection is poorly understood at the molecular level. Two different patterns of pulmonary host responses to virus were observed when gene expression profiles from severe acute respiratory syndrome coronavirus (SARS-CoV)-infected young mice that ...

journal_title：Journal of virology

authors： Baas T,Roberts A,Teal TH,Vogel L,Chen J,Tumpey TM,Katze MG,Subbarao K

更新日期：2008-10-01 00:00:00

abstract：:A potentially powerful approach for in vivo gene delivery is to target retrovirus to specific cells through interactions between cell surface receptors and appropriately modified viral envelope proteins. Previously, relatively large (>100 residues) protein ligands to cell surface receptors have been inserted at or nea...

journal_title：Journal of virology

authors： Gollan TJ,Green MR

更新日期：2002-04-01 00:00:00

abstract：:Hantavirus pulmonary syndrome (HPS) is a severe respiratory disease which is thought to result from a dysregulated immune response to infection with pathogenic hantaviruses, such as Sin Nombre virus or Andes virus (ANDV). Other New World hantaviruses, such as Prospect Hill virus (PHV), have not been associated with hu...

journal_title：Journal of virology

authors： Spiropoulou CF,Albariño CG,Ksiazek TG,Rollin PE

更新日期：2007-03-01 00:00:00

abstract：:Human T-cell lymphotropic virus type 1 (HTLV-1) enhances the growth of T lymphocytes, allowing the generation of T-lymphocyte cell lines. This report describes a limiting-dilution assay system which uses low input numbers of HTLV-1-producing cells for generation of T-lymphocyte cultures. The HTLV-1 transformants gener...

journal_title：Journal of virology

authors： Persaud D,Muñoz JL,Tarsis SL,Parks ES,Parks WP

更新日期：1995-10-01 00:00:00

abstract：UNLABELLED:Numerous experimental studies have demonstrated that CD8(+) T cells contribute to immunity against influenza by limiting viral replication. It is therefore surprising that rigorous statistical tests have failed to find evidence of positive selection in the epitopes targeted by CD8(+) T cells. Here we use a n...

journal_title：Journal of virology

authors： Machkovech HM,Bedford T,Suchard MA,Bloom JD

更新日期：2015-11-01 00:00:00

abstract：:Herpes simplex virus type 1 glycoprotein H (HSV-1 gH) was synthesized in an inducible mammalian cell expression system, and its properties were examined. The gH coding sequence, together with the stable 5' untranslated leader sequence from xenopus beta-globin, was placed under control of the strong promoter from the h...

journal_title：Journal of virology

authors： Gompels UA,Minson AC

更新日期：1989-11-01 00:00:00

abstract：:The dynamics of human immunodeficiency virus type 1 (HIV-1) transcription was analyzed in vitro and in vivo by using a specific molecular approach which allows accurate quantitation of the different classes of viral mRNAs. Unspliced (US) and multiply spliced (MS) HIV-1 transcripts were assayed by competitive reverse t...

journal_title：Journal of virology

authors： Bagnarelli P,Valenza A,Menzo S,Sampaolesi R,Varaldo PE,Butini L,Montroni M,Perno CF,Aquaro S,Mathez D,Leibowitch J,Balotta C,Clementi M

更新日期：1996-11-01 00:00:00