Abstract:
:A potentially powerful approach for in vivo gene delivery is to target retrovirus to specific cells through interactions between cell surface receptors and appropriately modified viral envelope proteins. Previously, relatively large (>100 residues) protein ligands to cell surface receptors have been inserted at or near the N terminus of retroviral envelope proteins. Although viral tropism could be altered, the chimeric envelope proteins lacked full activity, and coexpression of wild-type envelope was required for production of transducing virus. Here we analyze more than 40 derivatives of ecotropic Moloney murine leukemia virus (MLV) envelope, containing insertions of short RGD-containing peptides, which are ligands for integrin receptors. In many cases pseudotyped viruses containing only the chimeric envelope protein could transduce human cells. The precise location, size, and flanking sequences of the ligand affected transduction specificity and efficiency. We conclude that retroviral tropism can be rationally reengineered by insertion of short peptide ligands and without the need to coexpress wild-type envelope.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gollan TJ,Green MRdoi
10.1128/jvi.76.7.3558-3563.2002subject
Has Abstractpub_date
2002-04-01 00:00:00pages
3558-63issue
7eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
杂志文章abstract:UNLABELLED:Human T-cell lymphotropic virus type 1 (HTLV-1) and HTLV-2 encode auxiliary proteins that play important roles in viral replication, viral latency, and immune escape. The presence of auxiliary protein-encoding open reading frames (ORFs) in HTLV-3, the latest HTLV to be discovered, is unknown. Simian T-cell l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02150-14
更新日期:2015-01-15 00:00:00
abstract::The infection of cells which belong to the B-cell lineage is thought to be the primary event leading to the phenotypic and functional alterations seen in the murine AIDS (M. Huang, C. Simard, D. Kay, and P. Jolicoeur, J. Virol. 65:6562-6571, 1991). Using in situ hybridization, we studied the time course of the anatomi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1903-1912.1994
更新日期:1994-03-01 00:00:00
abstract::Entry of the budded virus form of baculoviruses into insect and mammalian cells is generally thought to occur through a low-pH-dependent endocytosis pathway, possibly through clathrin-coated pits. This insight is primarily based on (immuno)electron microscopy studies but requires biochemical support to exclude the use...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00880-06
更新日期:2006-09-01 00:00:00
abstract::The treatment of bacteriophage T4D with 2-hydroxy-5-nitrobenzyl bromide, a specific reagent for alkylating the indole ring of tryptophan residues, converts these particles from a cofactor-independent form to a cofactor-sensitive form. These treated T4D particles phenotypically resemble T4B particles in certain respect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.5.480-487.1968
更新日期:1968-05-01 00:00:00
abstract::Intramuscular administration of inactivated influenza virus vaccine is the main vaccine platform used for the prevention of seasonal influenza virus infection. In clinical trials, inactivated H5N1 vaccines have been shown to be safe and capable of eliciting immune correlates of protection. However, the H5N1 vaccines a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00596-10
更新日期:2010-09-01 00:00:00
abstract::We have found that interleukin-4 (IL-4) abrogated constitutive beta interferon (IFN-beta) synthesis in mouse macrophages. Analysis of endogenous IFN mRNA by reverse transcription-PCR clearly documented the presence of IFN-beta but not of IFN-alpha transcripts in RNA from bone marrow-derived macrophages (BMM). Culture ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6763-6766.1994
更新日期:1994-10-01 00:00:00
abstract:UNLABELLED:Although the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) epidemic was controlled by nonvaccine measures, coronaviruses remain a major threat to human health. The design of optimal coronavirus vaccines therefore remains a priority. Such vaccines present major challenges: coronavirus im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02980-14
更新日期:2015-03-01 00:00:00
abstract::Six nonoverlapping peptides of the neuraminidase (NA) glycoprotein of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8 virus) were found to be immunogenic for proliferating T cells when injected into BALB/c mice in Freund adjuvant. T cells elicited by peptide immunization could recognize PR8 virus in vitro. However, onl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.672-676.1991
更新日期:1991-02-01 00:00:00
abstract::Inhibition of histone deacetylase (HDAC) activity induces growth arrest, differentiation, and, in certain cell types, apoptosis. FR901228, FK228, or depsipeptide, is an HDAC inhibitor effective in T-cell lymphomas. Adult T-cell leukemia (ATL) is caused by human T-cell leukemia virus type 1 (HTLV-1) and remains incurab...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.78.9.4582-4590.2004
更新日期:2004-05-01 00:00:00
abstract::Among several proposed cellular receptors for bovine viral diarrhea virus (BVDV), the low-density lipoprotein (LDL) receptor is of special interest because it is also considered a receptor for the related hepatitis C virus. It has been reported that an anti-LDL receptor monoclonal antibody blocked the infection of bov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01589-06
更新日期:2006-11-01 00:00:00
abstract::In July 1991, an influenza A virus, designated A/Maryland/12/91 (A/MD), was isolated from the bronchial secretions of a 27-year-old animal caretaker. He had been admitted to the hospital with bilateral pneumonia and died of acute respiratory distress syndrome 13 days later. Antigenic analyses with postinfection ferret...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2051-2058.1994
更新日期:1994-04-01 00:00:00
abstract::The role of nitric oxide after poliovirus infection of the human HeLa (carcinoma) and U937 (promonocytic) cell lines has been analyzed. Both types of cells produced detectable levels of nitric oxide after poliovirus infection. However, this production was not sufficient to limit viral productivity. On the other hand, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2538-2540.1998
更新日期:1998-03-01 00:00:00
abstract::Experiments were conducted to investigate the roles of Marek's disease virus serotype 1 (MDV-1) major tegument proteins VP11/12, VP13/14, VP16, and VP22 in viral growth in cultured cells. Based on a bacterial artificial chromosome clone of MDV-1 (BAC20), mutant viruses were constructed in which the MDV-1 homologs of U...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1959-1970.2002
更新日期:2002-02-01 00:00:00
abstract::Adenovirus (Ad) entry into cells is initiated by the binding of the fiber knob to a cell surface receptor. The coxsackie- and adenovirus receptor (CAR) functions as the attachment receptor for many, but not all, Ad serotypes. Ad type 37 (Ad37), a subgroup D virus that causes keratoconjunctivitis in humans, does not in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5375-5380.2001
更新日期:2001-06-01 00:00:00
abstract::Duck hepatitis B virus mutants containing frameshift or stop codon mutations in a portion of the viral pol gene separating the terminal protein and reverse transcriptase domains had a leaky phenotype and, depending on the location and type of mutation, synthesized up to 10% as much viral DNA as did the wild type. This...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2155-2163.1991
更新日期:1991-05-01 00:00:00
abstract::The protein product of varicella-zoster virus (VZV) ORF47 is a serine/threonine protein kinase and tegument component. Evaluation of two recombinants of the Oka strain, rOka47DeltaC, with a C-terminal truncation of ORF47, and rOka47D-N, with a point mutation in the conserved kinase motif, showed that ORF47 kinase func...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13293-13305.2004
更新日期:2004-12-01 00:00:00
abstract::The mechanism of lysis by in vivo-induced cytotoxic T lymphocytes (CTL) was examined with virus-specific CTL from mice infected with lymphocytic choriomeningitis virus (LCMV). LCMV-induced T cells were shown to have greater than 10 times the serine esterase activity of T cells from normal mice, and high levels of seri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3726-3733.1990
更新日期:1990-08-01 00:00:00
abstract::The coronavirus nucleocapsid (N) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. These functions are likely to be facilitated by interactions with host cell proteins. The potential interactome of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00321-13
更新日期:2013-09-01 00:00:00
abstract::The feline immunodeficiency virus (FIV) cat model is extensively used to investigate possible vaccination approaches against AIDS in humans. Although consistent levels of protection have been achieved with FIV, as with other model systems, by immunizing with whole inactivated virus or fixed infected cells, the mechani...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.1-10.1999
更新日期:1999-01-01 00:00:00
abstract::Hepatitis C virus (HCV) chronic infection is characterized by low-level or undetectable cellular immune responses against HCV antigens. HCV proteins have been shown to affect various intracellular events and modulate immune responses, although the precise mechanisms used to mediate these effects are not fully understo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02547-07
更新日期:2008-04-01 00:00:00
abstract::We have identified a trans-dominant mutant form of the herpes simplex virus (HSV) DNA-binding protein ICP8 which inhibits viral replication. When expressed by the V2.6 cell line, the mutant gene product inhibited wild-type HSV production by 50- to 150-fold when the multiplicity of infection was less than 5. Production...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2666-2675.1991
更新日期:1991-05-01 00:00:00
abstract::The capsid (CA) domain of the Moloney murine leukemia virus (Mo-MuLV) Gag protein has a unique carboxy terminus with a highly charged arginine-rich sequence. Mutant viruses harboring arginine-to-alanine mutations affecting this region of CA displayed significant defects in virion release, and the few viral particles p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3339-3344.2003
更新日期:2003-03-01 00:00:00
abstract::Two live attenuated single-deletion mutant simian immunodeficiency virus (SIV) constructs, SIV239Deltanef and SIVPBj6.6Deltanef, were tested for their abilities to stimulate protective immunity in macaques. During the immunization period the animals were examined for specific immune responses and virus growth. Each co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1262-1270.1999
更新日期:1999-02-01 00:00:00
abstract::Wild-type bacteriophage phie and IS (interference-sensitive) mutants of the related phage SP82G did not productively infect strains of Bacillus subtilis that were lysogenic for temperate phage SPO2. In these abortive infections, the sensitive phages adsorbed to and penetrated the nonpermissive host, phage-directed mac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.839-847.1976
更新日期:1976-06-01 00:00:00
abstract::Three mRNAs from the murine polyomavirus early region encode the three well-characterized tumor antigens. We report the existence of a fourth alternatively spliced mRNA which encodes a fourth tumor antigen, tiny T antigen, which comprises the amino-terminal domain common to all of the T antigens but is extended by six...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.6068-6074.1997
更新日期:1997-08-01 00:00:00
abstract::BST-2/CD317/tetherin is a host factor that inhibits the release of HIV-1 and other unrelated viruses. A current model proposes that BST-2 physically tethers virions to the surface of virus-producing cells. The HIV-1-encoded Vpu protein effectively antagonizes the activity of BST-2. How Vpu accomplishes this task remai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02080-10
更新日期:2011-03-01 00:00:00
abstract::To further define the transcriptional regulation of the P38 promoter in the minute virus of mice (MVM) genome, we constructed a series of internal deletion and linker scanning mutations. The mutant P38 constructs were assayed for transcriptional activity in vitro by primer extension analysis with nuclear extracts from...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3776-3783.1992
更新日期:1992-06-01 00:00:00
abstract:UNLABELLED:The HIV-1 virion infectivity factor (Vif) targets the cellular cytidine deaminases APOBEC3G (A3G) and APOBEC3F (A3F) for degradation via the host ubiquitin-proteasome pathway. Vif recruits a cellular E3 ubiquitin ligase to polyubiquitinate A3G/F. The activity of Vif critically depends on the cellular core bi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03824-13
更新日期:2014-03-01 00:00:00
abstract::In vitro translation of the three single-stranded RNAs transcribed in vitro by bacteriophage phi 6 RNA polymerase revealed that the large RNA codes for phage proteins P1, P2, P4, and P7, the medium RNA codes for P3, P6, and P10, and the smaller RNA for P5, P8, and P9. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.249-251.1980
更新日期:1980-07-01 00:00:00
abstract::DNA vaccines have recently emerged at the forefront of approaches to harness the immune system in the prevention and treatment of viral infections, as well as the prevention and treatment of cancers. However, these vaccines suffer from limited efficacy since they often fail to produce significant antigen-specific CD8(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01954-09
更新日期:2010-03-01 00:00:00