Abstract:
:Six nonoverlapping peptides of the neuraminidase (NA) glycoprotein of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8 virus) were found to be immunogenic for proliferating T cells when injected into BALB/c mice in Freund adjuvant. T cells elicited by peptide immunization could recognize PR8 virus in vitro. However, only one of these peptides, corresponding to residues 79 to 93 of NA (NA 79-93), was able to restimulate T cells of mice immunized with infectious virus. T cells that recognized this peptide were uniformly I-Ed restricted, yet infectious influenza virus was required for responses. NA 79-93-specific T-hybridoma clones raised by immunization either with whole virus or with the synthetic peptide alone each responded to replicative virus and not to UV-inactivated virions. These data suggest that the NA 79-93 T-cell determinant which is commonly presented during an encounter with influenza virus in vivo is processed preferentially from NA synthesized within antigen-presenting cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hackett CJ,Horowitz D,Wysocka M,Eisenlohr LCdoi
10.1128/JVI.65.2.672-676.1991subject
Has Abstractpub_date
1991-02-01 00:00:00pages
672-6issue
2eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
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