Abstract:
:A human lymphoid cell line (F172-D8) excreting a human immunodeficiency virus type 1 (HIV-1) anti-gp41 monoclonal antibody was used to construct a plasmid containing the cDNA of the single-chain variable fragment (scFvD8) corresponding to this antibody. A stable human osteosarcoma cell line was obtained which expressed the scFvD8 protein in the cytoplasm. Whereas a cell line transfected with a control construct (pCI-neo) was readily and productively infected with laboratory (Ba-L) or primary HIV-1 isolates, the scFvD8 cell line did not support productive infection. Binding of the virus, internalization, and reverse transcription were not altered by scFvD8 expression, but gp160 expression was dramatically reduced. These data suggest that cytoplasmic expression of this artificial single-chain antibody can interfere with gp160 expression, thereby reducing the production of mature viral envelope proteins.
journal_name
J Viroljournal_title
Journal of virologyauthors
Legastelois I,Desgranges Cdoi
10.1128/jvi.74.12.5712-5715.2000subject
Has Abstractpub_date
2000-06-01 00:00:00pages
5712-5issue
12eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
杂志文章abstract::The C-type lectins DC-SIGN and DC-SIGNR [collectively referred to as DC-SIGN(R)] bind and transmit human immunodeficiency virus (HIV) and simian immunodeficiency virus to T cells via the viral envelope glycoprotein (Env). Other viruses containing heavily glycosylated glycoproteins (GPs) fail to interact with DC-SIGN(R...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1337-1346.2003
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journal_title:Journal of virology
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doi:10.1128/JVI.79.19.12528-12535.2005
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abstract::The Epstein-Barr virus (EBV) nuclear antigen 3C (EBNA-3C) protein is a transcriptional regulator of viral and cellular genes that is essential for EBV-mediated immortalization of B lymphocytes in vitro. EBNA-3C can inhibit transcription through an association with the cellular DNA-binding protein Jkappa, a function sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5151-5160.2000
更新日期:2000-06-01 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68 [also known as gammaHV-68]) is distinguished by its ability to replicate to high titers in cultured cells, making it an excellent candidate for studying gammaherpesvirus virion composition. Extracellular MHV68 virions were isolated, and abundant virion-associated proteins were identif...
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doi:10.1128/jvi.77.24.13425-13432.2003
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abstract::Although combination therapy allows the suppression of human immunodeficiency virus type 1 (HIV-1) viremia to undetectable levels, eradication has not been achieved because the virus persists in cellular reservoirs, particularly the latent reservoir in resting CD4(+) T lymphocytes. We previously established a simian i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01396-06
更新日期:2007-02-01 00:00:00
abstract::RNA-dependent RNA polymerase activity was found in mouse hepatitis virus strain A59 (MHV-A59)-infected cells. The enzyme was induced in the infected cells and could not be detected in the MHV-A59 virion. Two peaks of RNA polymerase activity, one early and the other late in infection, were detected. These polymerase ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.3.847-853.1982
更新日期:1982-06-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and high mortality rates in newborn piglets, leading to massive losses to the swine industry worldwide during recent epidemics. Intense research efforts are now focusing on defining viral characteristics that confer a growth advantage, pathogenicity, or cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01660-16
更新日期:2017-01-03 00:00:00
abstract:UNLABELLED:Enteroviruses (EVs) are a genetically and antigenically diverse group of viruses infecting humans. A mostly distinct set of EV variants have additionally been documented to infect wild apes and several, primarily captive, Old World monkey (OWM) species. To investigate the prevalence and genetic characteristi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00088-14
更新日期:2014-06-01 00:00:00
abstract::Previous findings of low levels of reverse transcriptase (RT) activity in chick cell-derived measles and mumps vaccines showed this activity to be associated with virus particles containing RNA of both subgroup E endogenous avian leukosis viruses (ALV-E) and endogenous avian viruses (EAV). These particles originate fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3605-3612.2001
更新日期:2001-04-01 00:00:00
abstract::Rous sarcoma virus (RSV), a simple retrovirus, needs to export unspliced viral RNA from the nucleus to the cytoplasm, circumventing the host cell restriction on cytoplasmic expression of intron-containing RNA. The cytoplasmic accumulation of full-length viral RNA is promoted by two cis-acting direct repeat (DR) elemen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9507-9514.2000
更新日期:2000-10-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) ICP34.5 gene is a neurovirulence gene in mice. In addition, some ICP34.5 mutants have been reported to have a reduced efficiency of induced reactivation as measured by in vitro explantation of latently infected mouse ganglia. However, since spontaneous reactivation is almost non...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.3033-3041.1995
更新日期:1995-05-01 00:00:00
abstract::A tetracycline-regulated gene expression system and a panel of novel monoclonal antibodies were used to examine the subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus (HCV) NS3-NS4A complex in inducible cell lines. The NS3 serine protease domain and the full-length NS3 protein ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2293-2304.2000
更新日期:2000-03-01 00:00:00
abstract::Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL) cells are predominantly infected with latent Kaposi's sarcoma-associated herpesvirus (KSHV), presenting a barrier to the destruction of tumor cells. Latent KSHV can be reactivated to undergo lytic replication. Here we report that in PEL cells, oxidative stress ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01742-10
更新日期:2011-01-01 00:00:00
abstract::Hemagglutinin from influenza A/PR8 virus was purified after treatment of the virus with sodium deoxycholate followed by extraction with tri-n-butyl phosphate. This fully disrupted the virus while preserving hemagglutinating activity. The hemagglutinin was obtained in the form of small aggregates that could be separate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.457-467.1978
更新日期:1978-05-01 00:00:00
abstract::CTCF and the cohesin complex modify chromatin by binding to DNA and interacting with each other and with other cellular proteins. Both proteins regulate transcription by a variety of local effects on transcription and by long-range topological effects. CTCF and cohesin also bind to herpesvirus genomes at specific site...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01279-19
更新日期:2020-01-06 00:00:00
abstract::Wild-type human respiratory syncytial virus (HRSV) is a poor inducer of alpha/beta interferons (IFN-alpha/beta). However, recombinant HRSV lacking the NS1 and NS2 genes (Delta NS1/2) induced high levels of IFN-alpha and -beta in human pulmonary epithelial cells (A549) as well as in macrophages derived from primary hum...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.8.4363-4369.2004
更新日期:2004-04-01 00:00:00
abstract::trans activation of the human immunodeficiency virus type 1 long terminal repeat requires that the viral trans activator Tat interact with the trans-acting responsive region (TAR) RNA. Although the N-terminal 47 amino acids represent an independent activation domain that functions via heterologous nucleic acid-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3441-3445.1993
更新日期:1993-06-01 00:00:00
abstract::The four major Epstein-Barr virion envelope components were separated by column chromatography and reconstituted into artificial liposomes. These liposomes were tested for their ability to bind selectively to Epstein-Barr virus receptor-positive cells. Only when the two high-molecular-weight glycoproteins, VE1 and VE2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.286-297.1982
更新日期:1982-01-01 00:00:00
abstract::During HIV-1 assembly, Gag polypeptides multimerize to form an immature capsid and also package HIV-1 genomic RNA. Assembling Gag forms immature capsids by progressing through a stepwise pathway of assembly intermediates containing the cellular ATPase ABCE1, which facilitates capsid formation. The NC domain of Gag is ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00288-11
更新日期:2011-07-01 00:00:00
abstract::Reverse transcription in hepatitis B viruses is initiated through a unique protein priming mechanism whereby the viral reverse transcriptase (RT) first assembles into a ribonucleoprotein (RNP) complex with its RNA template and then initiates DNA synthesis de novo using the RT itself as a protein primer. RNP formation ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11447-11455.2000
更新日期:2000-12-01 00:00:00
abstract::The mechanism of heparan sulfate (HS)-mediated human immunodeficiency virus type 1 (HIV-1) binding to and infection of T cells was investigated with a clone (H9h) of the T-cell line H9 selected on the basis of its high level of cell surface CD4 expression. Semiquantitative PCR analysis revealed that enzymatic removal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2233-2239.1995
更新日期:1995-04-01 00:00:00
abstract::Resistance to erythroleukemia induced by infection with the Friend virus complex (FV) has been mapped to several genes residing both within and outside the murine major histocompatibility complex (MHC). MHC genes located in the A, D, and Qa/Tla regions of the murine H-2 complex have been shown to affect disease resist...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.4921-4926.1994
更新日期:1994-08-01 00:00:00
abstract::Hepatitis C virus (HCV)-specific CD8 cell exhaustion may represent a mechanism of HCV persistence. The inhibitory receptor PD-1 has been reported to be up-regulated in exhausted CD8 cells. Therefore, we studied PD-1 expression longitudinally during acute HCV infection. Most HCV-specific CD8 cells expressed PD-1 at the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01177-06
更新日期:2006-11-01 00:00:00
abstract::The herpes simplex virus type 1 genome contains three origins of DNA replication: two copies of oriS and one copy of oriL. Although oriS has been characterized extensively, characterization of oriL has been severely limited by the inability to amplify oriL sequences in an undeleted form in Escherichia coli. We report ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1377-1388.1995
更新日期:1995-03-01 00:00:00
abstract::The emergence of drug resistance-conferring mutations can severely compromise the success of chemotherapy directed against human immunodeficiency virus type 1 (HIV-1). The M184V and/or L74V mutation in the reverse transcriptase (RT) gene are frequently found in viral isolates from patients treated with the nucleoside ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.16.8621-8632.2003
更新日期:2003-08-01 00:00:00
abstract::Endocytosis and endosomal trafficking regulate the proteins targeted to the plasma membrane and play essential roles in diverse cellular processes, including responses to pathogen attack. Here, we report the identification of Glycine max (soybean) endocytosis dynamin-like protein 5A (GmSDL5A) associated with purified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01320-18
更新日期:2018-11-12 00:00:00
abstract::The role of nitric oxide after poliovirus infection of the human HeLa (carcinoma) and U937 (promonocytic) cell lines has been analyzed. Both types of cells produced detectable levels of nitric oxide after poliovirus infection. However, this production was not sufficient to limit viral productivity. On the other hand, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2538-2540.1998
更新日期:1998-03-01 00:00:00
abstract::Previous work on the strict late (gamma) UL38 promoter of herpes simplex virus type 1 identified three cis-acting elements required for wild-type levels of transcription: a TATA box at -31, a consensus mammalian initiator element at the transcription start site, and a downstream activation sequence (DAS) at +20 to +33...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8181-8190.1998
更新日期:1998-10-01 00:00:00
abstract:UNLABELLED:The cytomegaloviruses (CMVs) are among the most genetically complex mammalian viruses, with viral genomes that often exceed 230 kbp. Manipulation of cytomegalovirus genomes is largely performed using infectious bacterial artificial chromosomes (BACs), which necessitates the maintenance of the viral genome in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00139-16
更新日期:2016-07-11 00:00:00
abstract::A virulent porcine epidemic diarrhea virus (PEDV) strain, DR13, was obtained from suckling pigs suspected of having porcine epidemic diarrhea in 1999 in Korea, and its attenuated counterpart was derived from virulent strain DR13 by serial propagation in Vero cells. This report describes the first complete genome seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00557-12
更新日期:2012-05-01 00:00:00