Abstract:
:Marburg virus, a filovirus, causes severe hemorrhagic fever with hitherto poorly understood molecular pathogenesis. We have investigated here the vectorial transport of the surface protein GP of Marburg virus in polarized epithelial cells. To this end, we established an MDCKII cell line that was able to express GP permanently (MDCK-GP). The functional integrity of GP expressed in these cells was analyzed using vesicular stomatitis virus pseudotypes. Further experiments revealed that GP is transported in MDCK-GP cells mainly to the apical membrane and is released exclusively into the culture medium facing the apical membrane. When MDCKII cells were infected with Marburg virus, the majority of GP was also transported to the apical membrane, suggesting that the protein contains an autonomous apical transport signal. Release of infectious progeny virions, however, took place exclusively at the basolateral membrane of the cells. Thus, vectorial budding of Marburg virus is presumably determined by factors other than the surface protein.
journal_name
J Viroljournal_title
Journal of virologyauthors
Sänger C,Mühlberger E,Ryabchikova E,Kolesnikova L,Klenk HD,Becker Sdoi
10.1128/JVI.75.3.1274-1283.2001subject
Has Abstractpub_date
2001-02-01 00:00:00pages
1274-83issue
3eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
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