Removal of carbohydrate from influenza A virus and its hemagglutinin and the effect on biological activities.

abstract：:The C-terminal domain of human immunodeficiency virus type 1 (HIV-1) integrase (IN) is a dimer that binds to DNA in a nonspecific manner. The structure of the minimal region required for DNA binding (IN220-270) has been solved by nuclear magnetic resonance spectroscopy. The overall fold of the C-terminal domain of HIV...

journal_title：Journal of virology

authors： Lutzke RA,Plasterk RH

更新日期：1998-06-01 00:00:00

abstract：:Scrapie-associated fibrils (SAFs) are abnormal filamentous structures that are uniquely associated with unconventional slow virus diseases. The antigenic relationships of SAFs from animals infected with four biologically distinct scrapie strains were investigated by using antisera raised to purified SAF proteins. Rabb...

journal_title：Journal of virology

authors： Kascsak RJ,Rubenstein R,Merz PA,Carp RI,Robakis NK,Wisniewski HM,Diringer H

更新日期：1986-09-01 00:00:00

abstract：:The genomic RNA 3' noncoding region is believed to be a major cis-acting molecular genetic determinant for regulating picornavirus negative-strand RNA synthesis by promoting replication complex recognition. We report the replication of two picornavirus RNAs harboring complete deletions of the genomic RNA 3' noncoding ...

journal_title：Journal of virology

authors： Todd S,Towner JS,Brown DM,Semler BL

更新日期：1997-11-01 00:00:00

abstract：:The envelope of human parainfluenza virus type 3 (HPF3) contains two viral glycoproteins, the hemagglutinin-neuraminidase (HN) and the fusion protein (F). HN, which is responsible for receptor attachment and for promoting F-mediated fusion, also possesses neuraminidase (receptor-destroying) activity. We reported previ...

journal_title：Journal of virology

authors： Porotto M,Greengard O,Poltoratskaia N,Horga MA,Moscona A

更新日期：2001-08-01 00:00:00

abstract：:Treatment of polyoma virions with ethyleneglycol-bil-N,N'-tetraacetic acid (EGTA) and dithiothreitol (DTT) at pH 8.5 resulted in the dissociation of the virions into a DNA-protein complex and individual structural capsomere subunits. The sedimentation value of the DNA-protein complex in sucrose gradients was approxima...

journal_title：Journal of virology

authors： Brady JN,Winston VD,Consigli RA

更新日期：1978-07-01 00:00:00

abstract：:Diverse members of the Paramyxovirus family of negative-strand RNA viruses effectively suppress host innate immune responses through the actions of their V proteins. The V protein mediates interference with the interferon regulatory RNA helicase MDA5 to avoid cellular antiviral responses. Analysis of the interaction i...

journal_title：Journal of virology

authors： Parisien JP,Bamming D,Komuro A,Ramachandran A,Rodriguez JJ,Barber G,Wojahn RD,Horvath CM

更新日期：2009-07-01 00:00:00

abstract：:Nucleotide substitution mutagenesis was conducted to investigate the importance of the inverted repeats (palindrome) at the origin of DNA replication (Ori) of porcine circovirus type 1 (PCV1). Viral genomes with engineered mutations on either arm or both arms of the palindrome were not impaired in protein synthesis an...

journal_title：Journal of virology

authors： Cheung AK

更新日期：2004-04-01 00:00:00

abstract：:Assembly and maturation of retroviral particles requires the aggregation and controlled proteolytic cleavage of polyprotein core precursors by a precursor-encoded protease (PR). Active, mature retroviral PR is a dimer, and the accumulation of precursors at sites of assembly may facilitate subunit interaction and subse...

journal_title：Journal of virology

authors： Burstein H,Bizub D,Skalka AM

更新日期：1991-11-01 00:00:00

abstract：:During secondary immune responses to influenza virus, virus-specific T memory cells are a major source of gamma interferon (IFN-gamma). We assessed the contribution of IFN-gamma to heterologous protection against the A/WSN/33 (H1N1) virus of wild-type and IFN-gamma-/- mice previously immunized with the A/HK/68 (H3N2) ...

journal_title：Journal of virology

authors： Bot A,Bot S,Bona CA

更新日期：1998-08-01 00:00:00

abstract：:The integrin alpha(v)beta3 has been shown to act as the receptor for internalization of foot-and-mouth disease virus (FMDV) (A12), with attachment being through a highly conserved RGD motif located on the G-H loop of viral capsid protein VP1. In addition, however, we have recently shown that efficient infection of cul...

journal_title：Journal of virology

authors： Jackson T,Sharma A,Ghazaleh RA,Blakemore WE,Ellard FM,Simmons DL,Newman JW,Stuart DI,King AM

更新日期：1997-11-01 00:00:00

abstract：:Poliovirus (PV) is easily transferred to humans orally; however, no rodent model for oral infections has been developed because of the alimentary tract's low sensitivity to the virus. Here we showed that PV is inactivated by the low pH of the gastric contents in mice. The addition of 3% NaHCO3 to the viral inoculum in...

journal_title：Journal of virology

authors： Ohka S,Igarashi H,Nagata N,Sakai M,Koike S,Nochi T,Kiyono H,Nomoto A

更新日期：2007-08-01 00:00:00

abstract：:The UL3 protein of equine herpesvirus 1 (EHV-1) KyA strain is a homolog of the ICP27 alpha regulatory protein of herpes simplex virus type 1 (HSV-1) and the ORF 4 protein of varicella-zoster virus. To characterize the regulatory function of the UL3 gene product, a UL3 gene expression vector (pSVUL3) and a vector expre...

journal_title：Journal of virology

authors： Zhao Y,Holden VR,Smith RH,O'Callaghan DJ

更新日期：1995-05-01 00:00:00

abstract：:Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe acute respiratory infection with as yet unclear epidemiology. We previously showed that MERS-CoV counteracts parts of the innate immune response in human bronchiolar cells. Here we analyzed accessory proteins 3, 4a, 4b, and 5 for their abilities to ...

journal_title：Journal of virology

authors： Niemeyer D,Zillinger T,Muth D,Zielecki F,Horvath G,Suliman T,Barchet W,Weber F,Drosten C,Müller MA

更新日期：2013-11-01 00:00:00

abstract：:A detailed kinetic and quantitative analysis of the early and late biosynthetic events undergone by the human immunodeficiency virus type 1 envelope protein expressed by a recombinant vaccinia virus was performed. Early folding events that occurred in the endoplasmic reticulum included disulfide bond formation (t1/2 a...

journal_title：Journal of virology

authors： Earl PL,Moss B,Doms RW

更新日期：1991-04-01 00:00:00

abstract：:Reovirus virions are nonenveloped icosahedral particles consisting of two concentric protein shells, termed outer capsid and core. Outer-capsid protein sigma1 is the viral attachment protein and binds carbohydrate molecules on the surface of host cells. Monoclonal antibody (MAb) 4F2, which is specific for outer-capsid...

journal_title：Journal of virology

authors： Nason EL,Wetzel JD,Mukherjee SK,Barton ES,Prasad BV,Dermody TS

更新日期：2001-07-01 00:00:00

abstract：:Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by tra...

journal_title：Journal of virology

authors： Gilbert J,Ou W,Silver J,Benjamin T

更新日期：2006-11-01 00:00:00

abstract：:B cells secreting IgG antibodies, but not IgM, are thought to be solely responsible for vaccine-induced protection against rabies virus (RABV) infections in postexposure settings. In this report, we reinvestigated the potential for IgM to mediate protection in a mouse model of RABV vaccination. Immunocompetent mice im...

journal_title：Journal of virology

authors： Dorfmeier CL,Shen S,Tzvetkov EP,McGettigan JP

更新日期：2013-08-01 00:00:00

abstract：:Recent studies have proposed that adeno-associated viruses (AAVs) are not evolutionarily linked to other mammalian autonomous parvoviruses but are more closely linked to the autonomous parvoviruses of birds. To better understand the relationship between primate and avian AAVs (AAAVs), we cloned and sequenced the genom...

journal_title：Journal of virology

authors： Bossis I,Chiorini JA

更新日期：2003-06-01 00:00:00

abstract：:MicroRNAs (miRNAs) are potent RNA regulators of gene expression. Some viruses encode miRNAs, most of unknown function. The majority of viral miRNAs are not conserved, and whether any have conserved functions remains unclear. Here, we report that two human polyomaviruses associated with serious disease in immunocomprom...

journal_title：Journal of virology

authors： Seo GJ,Fink LH,O'Hara B,Atwood WJ,Sullivan CS

更新日期：2008-10-01 00:00:00

abstract：:In this study, we characterized the viral determinants of coreceptor usage in relation to susceptibility to antibody-mediated neutralization or enhancement of infectivity by using chimeras of three highly related human immunodeficiency virus type 1 (HIV-1) isolates of different phenotypes. We found that the V3 region ...

journal_title：Journal of virology

authors： Guillon C,Schutten M,Boers PH,Gruters RA,Osterhaus AD

更新日期：2002-03-01 00:00:00

abstract：:Polyamine depletion produced by exogenous arginine in Escherichia coliK-12 cultures defective in agmatine ureohydrolase activity resulted in a marked inhibition of the rates of growth and nucleic acid synthesis. Addition of putrescine or spermidine to such depleted cultures restored the control rate of growth and nucl...

journal_title：Journal of virology

authors： Dion AS,Cohen SS

更新日期：1972-03-01 00:00:00

abstract：:The adenovirus E1A 243R oncoprotein is capable of transactivating the expression of the human proliferating cell nuclear antigen (PCNA) promoter. Mutational analysis of the E1A 243R protein suggested that both its p300/CBP- and p107-binding regions are required for optimal induction of the PCNA promoter (C. Kannabiran...

journal_title：Journal of virology

authors： Lee BH,Liu M,Mathews MB

更新日期：1998-02-01 00:00:00

abstract：:Epstein-Barr virus (EBV) SM protein is an essential nuclear shuttling protein expressed by EBV early during the lytic phase of replication. SM acts to increase EBV lytic gene expression by binding EBV mRNAs and enhancing accumulation of the majority of EBV lytic cycle mRNAs. SM increases target mRNA stability and nucl...

journal_title：Journal of virology

authors： Han Z,Verma D,Hilscher C,Dittmer DP,Swaminathan S

更新日期：2009-11-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is typically acquired asymptomatically in childhood. In contrast, infection later in life often leads to infectious mononucleosis (IM), a febrile illness characterized by anti-EBV IgM antibody positivity, high loads of circulating latently infected B cells, and a marked lymphocytosis caused by...

journal_title：Journal of virology

authors： Abbott RJ,Pachnio A,Pedroza-Pacheco I,Leese AM,Begum J,Long HM,Croom-Carter D,Stacey A,Moss PAH,Hislop AD,Borrow P,Rickinson AB,Bell AI

更新日期：2017-10-13 00:00:00

abstract：:An in vitro system is described for the assembly of herpes simplex virus type 1 (HSV-1) procapsids beginning with three purified components, the major capsid protein (VP5), the triplexes (VP19C plus VP23), and a hybrid scaffolding protein. Each component was purified from insect cells expressing the relevant protein(s...

journal_title：Journal of virology

authors： Newcomb WW,Homa FL,Thomsen DR,Trus BL,Cheng N,Steven A,Booy F,Brown JC

更新日期：1999-05-01 00:00:00

abstract：:We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 ...

journal_title：Journal of virology

authors： Flores J,Sears J,Schael IP,White L,Garcia D,Lanata C,Kapikian AZ

更新日期：1990-08-01 00:00:00

abstract：:The family Geminiviridae comprises seven genera differentiated by genome organization, sequence similarity, and insect vector. Capulavirus, an eighth genus, has been proposed to accommodate two newly discovered highly divergent geminiviruses that presently have no known vector. Alfalfa leaf curl virus, identified here...

journal_title：Journal of virology

authors： Roumagnac P,Granier M,Bernardo P,Deshoux M,Ferdinand R,Galzi S,Fernandez E,Julian C,Abt I,Filloux D,Mesléard F,Varsani A,Blanc S,Martin DP,Peterschmitt M

更新日期：2015-09-01 00:00:00

abstract：:The occurrence of genome segment reassortment between two antigenically related orbiviruses was demonstrated in cattle. Individual virus clones were isolated by cell culture plaque assays directly from the blood of a calf infected with two serotypes of bluetongue virus. The majority (89%) of progeny viruses isolated f...

journal_title：Journal of virology

authors： Stott JL,Oberst RD,Channell MB,Osburn BI

更新日期：1987-09-01 00:00:00

abstract：:Poliovirus-specific T lymphocytes were isolated from virus-immunized mice of different H-2 haplotypes. Immunological characterization of this population indicates that the effector population involved in the observed poliovirus-specific proliferative response was that of CD4-positive T-helper cells. Proliferative resp...

journal_title：Journal of virology

authors： Kutubuddin M,Simons J,Chow M

更新日期：1992-05-01 00:00:00

abstract：:The reasons for poor CD4+ T-cell recovery in some human immunodeficiency virus (HIV)-infected subjects despite effective highly active antiretroviral therapy (HAART) remain unclear. We recently reported that CXCR4-using (X4) HIV-1 could be gradually selected in cellular reservoirs during sustained HAART. Because of th...

journal_title：Journal of virology

authors： Delobel P,Nugeyre MT,Cazabat M,Sandres-Sauné K,Pasquier C,Cuzin L,Marchou B,Massip P,Cheynier R,Barré-Sinoussi F,Izopet J,Israël N

更新日期：2006-10-01 00:00:00