当前位置： SCI文献检索 > JOURNAL OF VIROLOGY期刊下所有文献 > Folding, interaction with GRP78-BiP, assembly, and transport of the human immunodeficiency virus type 1 envelope protein.

Folding, interaction with GRP78-BiP, assembly, and transport of the human immunodeficiency virus type 1 envelope protein.

Abstract：

:A detailed kinetic and quantitative analysis of the early and late biosynthetic events undergone by the human immunodeficiency virus type 1 envelope protein expressed by a recombinant vaccinia virus was performed. Early folding events that occurred in the endoplasmic reticulum included disulfide bond formation (t1/2 approximately 10 min), folding of envelope protein into a form competent to bind CD4 (t1/2 approximately 15 min), and specific and transient association and dissociation with GRP78-BiP (t1/2 approximately 25 min). After initial folding, envelope protein monomers formed noncovalently associated dimers with high efficiency (t1/2 approximately 30 min). Studies with brefeldin A, a compound that inhibits endoplasmic reticulum-to-Golgi transport, suggested that assembly occurred in the endoplasmic reticulum while cleavage of gp160 into gp120/gp41 subunits occurred in a post-endoplasmic reticulum compartment. Transport to the Golgi was monitored by modification of N-linked sugars to forms partially resistant to endoglycosidase H. The kinetics of endoglycosidase H resistance were nearly identical to the kinetics of gp160 cleavage (t1/2 approximately 80 min). Cleavage efficiency was strongly cell type dependent, ranging from 13 to 70%. By contrast, approximately 50% of the gp120 generated by the cleavage event was shed (t1/2 approximately 120 min) regardless of the cell type used. The results are discussed in terms of the overall biosynthetic pathway of the envelope protein and provide a framework with which to assess the effects of mutations on structure and function.

journal_name

J Virol

journal_title

Journal of virology

authors

Earl PL,Moss B,Doms RW

doi

10.1128/JVI.65.4.2047-2055.1991

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

2047-55

issue

4

eissn

0022-538X

issn

1098-5514

journal_volume

65

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGY文献大全
  • Unique species of mRNA from adenovirus type 7 early region 1 in cells transformed by adenovirus type 7 DNA fragment.

    abstract：:Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I . J fragment (the left 7.8% of the viral genome) and in human KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.36.2.337-352.1980

    authors： Yoshida K,Fujinaga K

    更新日期：1980-11-01 00:00:00

  • VPg gene amplification correlates with infective particle formation in foot-and-mouth disease virus.

    abstract：:In order to analyze the function of VPg amplification in aphthoviruses, we have undertaken the first mutational analysis of the repetitive VPg-coding region using an improved foot-and-mouth disease virus (FMDV) cDNA clone from which infective viral RNA was synthesized. A set of VPg mutants was constructed by site-dire...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.66.4.2251-2260.1992

    authors： Falk MM,Sobrino F,Beck E

    更新日期：1992-04-01 00:00:00

  • Vaccinia virus gene encoding a 30-kilodalton subunit of the viral DNA-dependent RNA polymerase.

    abstract：:Antibody was raised against purified vaccinia virus RNA polymerase and used to screen a recombinant vaccinia virus-lambda gt11 library. The DNA from several immunopositive clones was shown by Southern hybridization to originate from the vaccinia virus HindIII E fragment. The nucleotide sequence of the RNA polymerase s...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.64.11.5376-5382.1990

    authors： Broyles SS,Pennington MJ

    更新日期：1990-11-01 00:00:00

  • The influenza A virus PB2, PA, NP, and M segments play a pivotal role during genome packaging.

    abstract：:The genomes of influenza A viruses consist of eight negative-strand RNA segments. Recent studies suggest that influenza viruses are able to specifically package their segmented genomes into the progeny virions. Segment-specific packaging signals of influenza virus RNAs (vRNAs) are located in the 5' and 3' noncoding re...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00662-12

    authors： Gao Q,Chou YY,Doğanay S,Vafabakhsh R,Ha T,Palese P

    更新日期：2012-07-01 00:00:00

  • Functional characterization of Xenopus laevis p53: evidence of temperature-sensitive transactivation but not of repression.

    abstract：:We have investigated the effect of Xenopus laevis p53 (Xp53) on transcription from a variety of promoters which are regulated by mouse p53 using a chloramphenicol acetyltransferase reporter system. Although Xp53 transactivated promoters that are up-regulated by mouse p53, it was unable to cause repression. This abilit...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.68.11.7178-7187.1994

    authors： Ridgway PJ,Soussi T,Braithwaite AW

    更新日期：1994-11-01 00:00:00

  • Sequence and structure alignment of Paramyxoviridae attachment proteins and discovery of enzymatic activity for a morbillivirus hemagglutinin.

    abstract：:On the basis of the conservation of neuraminidase (N) active-site residues in influenza virus N and paramyxovirus hemagglutinin-neuraminidase (HN), it has been suggested that the three-dimensional (3D) structures of the globular heads of the two proteins are broadly similar. In this study, details of this structural s...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.71.8.6155-6167.1997

    authors： Langedijk JP,Daus FJ,van Oirschot JT

    更新日期：1997-08-01 00:00:00

  • The Kaposi's sarcoma-associated herpesvirus K12 transcript from a primary effusion lymphoma contains complex repeat elements, is spliced, and initiates from a novel promoter.

    abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) latently infects KS tumors, primary effusion lymphomas (PELs), and PEL cell lines. K12 (T0.7) is the most abundant transcript expressed in latent KSHV infection. We characterize here the K12 transcript from a PEL tumor prior to passage in cell culture. The PEL tumor KSHV ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.76.23.11880-11888.2002

    authors： Li H,Komatsu T,Dezube BJ,Kaye KM

    更新日期：2002-12-01 00:00:00

  • Replication of hepatitis delta virus RNA: effect of mutations of the autocatalytic cleavage sites.

    abstract：:Hepatitis delta virus (HDV) contains a circular RNA genome of 1.7 kb. HDV RNA replication is thought to proceed via a rolling-circle mechanism that is dependent on autocatalytic cleavage and ligation reactions. However, it has never been established that these ribozyme activities are indeed involved in HDV RNA replica...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.67.4.2228-2234.1993

    authors： Macnaughton TB,Wang YJ,Lai MM

    更新日期：1993-04-01 00:00:00

  • Origin of polysaccharide depolymerase associated with bacteriophage infection.

    abstract：:Analyses, by construction of phage growth curves, indicated that the polysaccharide depolymerase was synthesized by Pseudomonas aeruginosa strains B and BI after infection with phage 2. The kinetics of biosynthesis of the depolymerase were found to parallel closely the rate of formation of phage-directed virions, and ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.3.3.290-296.1969

    authors： Bartell PF,Orr TE

    更新日期：1969-03-01 00:00:00

  • JC virus DNA is present in many human brain samples from patients without progressive multifocal leukoencephalopathy.

    abstract：:Sections of normal and diseased brain and kidney tissues were screened for the presence of JC virus (JCV) DNA by using the polymerase chain reaction. As expected, all samples obtained from patients with progressive multifocal leukoencephalopathy (PML) tested positive when multiple JCV-specific primer and probe combina...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.66.10.5726-5734.1992

    authors： White FA 3rd,Ishaq M,Stoner GL,Frisque RJ

    更新日期：1992-10-01 00:00:00

  • RNA- temperature-sensitive mutants of vesicular stomatitis virus: L-protein thermosensitivity accounts for transcriptase restriction of group I mutants.

    abstract：:In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted trans...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.18.2.596-603.1976

    authors： Hunt DM,Emerson SU,Wagner RR

    更新日期：1976-05-01 00:00:00

  • New world clade B arenaviruses can use transferrin receptor 1 (TfR1)-dependent and -independent entry pathways, and glycoproteins from human pathogenic strains are associated with the use of TfR1.

    abstract：:Arenaviruses are rodent-borne viruses, with five members of the family capable of causing severe hemorrhagic fevers if transmitted to humans. To date, two distinct cellular receptors have been identified that are used by different pathogenic viruses, alpha-dystroglycan by Lassa fever virus and transferrin receptor 1 (...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01397-07

    authors： Flanagan ML,Oldenburg J,Reignier T,Holt N,Hamilton GA,Martin VK,Cannon PM

    更新日期：2008-01-01 00:00:00

  • In transduced cells, the US3 protein kinase of herpes simplex virus 1 precludes activation and induction of apoptosis by transfected procaspase 3.

    abstract：:The US3 protein kinase of herpes simplex virus 1 blocks apoptosis induced by replication-incompetent virus mutants, proapoptotic members of the Bcl-2 family of proteins, and by a variety of other agents that act at the premitochondrial level in the proapoptotic cascade. To define the role of US3 in blocking apoptosis ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00820-07

    authors： Benetti L,Roizman B

    更新日期：2007-10-01 00:00:00

  • Isolation and propagation of human papillomavirus type 16 in human xenografts implanted in the severe combined immunodeficiency mouse.

    abstract：:We report the isolation and propagation of human papillomavirus type 16, the main agent of cervical cancer, using human foreskin fragments implanted in severe combined immunodeficiency mice. The infection produced viral particles, and with each passage of the virus it caused lesions identical to intraepithelial neopla...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.72.6.5256-5261.1998

    authors： Bonnez W,DaRin C,Borkhuis C,de Mesy Jensen K,Reichman RC,Rose RC

    更新日期：1998-06-01 00:00:00

  • Chromosomal localization of the Moloney sarcoma virus mouse cellular (c-mos) sequence.

    abstract：:The Moloney sarcoma virus-specific onc gene, referred to as v-mos, was used as probe to hybridize to restricted DNAs from various mouse-Chinese hamster hybrid cell lines. These hybrid cells contain, in addition to all of the Chinese hamster chromosomes, various numbers (less than a full complement) of mouse chromosome...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.44.2.752-754.1982

    authors： Swan D,Oskarsson M,Keithley D,Ruddle FH,D'Eustachio P,Vande Woude GF

    更新日期：1982-11-01 00:00:00

  • Selective replication of coronavirus genomes that express nucleocapsid protein.

    abstract：:The coronavirus nucleocapsid (N) protein is a structural protein that forms a ribonucleoprotein complex with genomic RNA. In addition to its structural role, it has been described as an RNA-binding protein that might be involved in coronavirus RNA synthesis. Here, we report a reverse genetic approach to elucidate the ...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.79.11.6620-6630.2005

    authors： Schelle B,Karl N,Ludewig B,Siddell SG,Thiel V

    更新日期：2005-06-01 00:00:00

  • Regulation of autophagic activation by Rta of Epstein-Barr virus via the extracellular signal-regulated kinase pathway.

    abstract：:Autophagy is an intracellular degradation pathway that provides a host defense mechanism against intracellular pathogens. However, many viruses exploit this mechanism to promote their replication. This study shows that lytic induction of Epstein-Barr virus (EBV) increases the membrane-bound form of LC3 (LC3-II) and LC...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02033-14

    authors： Hung CH,Chen LW,Wang WH,Chang PJ,Chiu YF,Hung CC,Lin YJ,Liou JY,Tsai WJ,Hung CL,Liu ST

    更新日期：2014-10-01 00:00:00

  • Conservation of RNA-protein interactions among picornaviruses.

    abstract：:Picornavirus genomes encode unique 5' noncoding regions (5' NCRs) which are approximately 600 to 1,300 nucleotides in length, contain multiple upstream AUG codons, and display the ability to form extensive secondary structures. A number of recent reports have shown that picornavirus 5' NCRs are able to facilitate cap-...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.66.7.4364-4376.1992

    authors： Dildine SL,Semler BL

    更新日期：1992-07-01 00:00:00

  • Expansion of human T-cell leukemia virus type 1 (HTLV-1) reservoir in orally infected rats: inverse correlation with HTLV-1-specific cellular immune response.

    abstract：:Adult T-cell leukemia (ATL) occurs in a small population of human T-cell leukemia virus type 1 (HTLV-1)-infected individuals. Although the critical risk factor for ATL development is not clear, it has been noted that ATL is incidentally associated with mother-to-child infection, elevated proviral loads, and weakness i...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/jvi.77.5.2956-2963.2003

    authors： Hasegawa A,Ohashi T,Hanabuchi S,Kato H,Takemura F,Masuda T,Kannagi M

    更新日期：2003-03-01 00:00:00

  • Virus factories of cauliflower mosaic virus are virion reservoirs that engage actively in vector transmission.

    abstract：:Cauliflower mosaic virus (CaMV) forms two types of inclusion bodies within infected plant cells: numerous virus factories, which are the sites for viral replication and virion assembly, and a single transmission body (TB), which is specialized for virus transmission by aphid vectors. The TB reacts within seconds to ap...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.01883-13

    authors： Bak A,Gargani D,Macia JL,Malouvet E,Vernerey MS,Blanc S,Drucker M

    更新日期：2013-11-01 00:00:00

  • Severe acute respiratory syndrome coronavirus protein 6 accelerates murine hepatitis virus infections by more than one mechanism.

    abstract：:The severe acute respiratory syndrome coronavirus (SARS-CoV) encodes numerous accessory proteins whose importance in the natural infection process is currently unclear. One of these accessory proteins is set apart by its function in the context of a related murine hepatitis virus (MHV) infection. SARS-CoV protein 6 in...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.02406-07

    authors： Hussain S,Perlman S,Gallagher TM

    更新日期：2008-07-01 00:00:00

  • Monoclonal antibodies against influenza virus PB2 and NP polypeptides interfere with the initiation step of viral mRNA synthesis in vitro.

    abstract：:Two panels of monoclonal antibodies (MAbs) specific for the influenza A virus PA and PB2 polypeptides have been obtained from mice immunized with denatured proteins produced in Escherichia coli. All MAbs (13 specific for the PA polypeptide and 8 specific for the PB2 protein) reacted to the corresponding influenza viru...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.68.11.6900-6909.1994

    authors： Bárcena J,Ochoa M,de la Luna S,Melero JA,Nieto A,Ortín J,Portela A

    更新日期：1994-11-01 00:00:00

  • A 348-base-pair region in the latency-associated transcript facilitates herpes simplex virus type 1 reactivation.

    abstract：:Latency-associated transcript (LAT) promoter deletion mutants of herpes simplex virus type 1 have a reduced capacity to reactivate following adrenergic induction in the rabbit eye model. We have mapped a reactivation phenotype within LAT and describe the construction of recombinants in which poly(A) addition sites hav...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.70.4.2449-2459.1996

    authors： Bloom DC,Hill JM,Devi-Rao G,Wagner EK,Feldman LT,Stevens JG

    更新日期：1996-04-01 00:00:00

  • Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine.

    abstract：:Swine influenza viruses (SIV) naturally infect pigs and can be transmitted to humans. In the pig, genetic reassortment to create novel influenza subtypes by mixing avian, human, and swine influenza viruses is possible. An SIV vaccine inducing cross-protective immunity between different subtypes and strains circulating...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00787-06

    authors： Richt JA,Lekcharoensuk P,Lager KM,Vincent AL,Loiacono CM,Janke BH,Wu WH,Yoon KJ,Webby RJ,Solórzano A,García-Sastre A

    更新日期：2006-11-01 00:00:00

  • Protection of chimpanzees from type B hepatitis by immunization with woodchuck hepatitis virus surface antigen.

    abstract：:Two chimpanzees immunized with woodchuck hepatitis virus (WHV) surface antigen (WHsAg) developed antibodies cross-reactive with hepatitis B virus (HBV) surface antigen (HBsAg). After challenge with HBV, one animal was completely protected and the other experienced a subclinical infection, without evidence of liver dis...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.60.3.895-901.1986

    authors： Cote PJ,Shapiro M,Engle RE,Popper H,Purcell RH,Gerin JL

    更新日期：1986-12-01 00:00:00

  • A single amino acid substitution in the murine norovirus capsid protein is sufficient for attenuation in vivo.

    abstract：:Murine norovirus (MNV), a prevalent pathogen of laboratory mice, shares many characteristics with human noroviruses. Previous results indicated that passage of MNV1 in the macrophage cell line RAW 264.7 results in attenuation in STAT1-deficient mice (C. E. Wobus, S. M. Karst, L. B. Thackray, K. O. Chang, S. V. Sosnovt...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.00237-08

    authors： Bailey D,Thackray LB,Goodfellow IG

    更新日期：2008-08-01 00:00:00

  • Antiviral activity of polyacrylic and polymethacrylic acids. II. Mode of action in vivo.

    abstract：:A marked virus-inhibiting potency is obtained in the serum after intraperitoneal injection of polyacrylic acid (PAA) and polymethacrylic acid (PMAA) in mice. Much higher antiviral levels were reached than for other related polymers including dextran sulfate, heparin, polyvinyl sulfate, pyran copolymer, polystyrene sul...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.2.9.886-893.1968

    authors： De Somer P,De Clercq E,Billiau A,Schonne E,Claesen M

    更新日期：1968-09-01 00:00:00

  • The human immunodeficiency virus type 1 capsid p2 domain confers sensitivity to the cyclophilin-binding drug SDZ NIM 811.

    abstract：:Human immunodeficiency virus type 1 (HIV-1) specifically incorporates the host cell peptidyl-prolyl isomerase cyclophilin A into virions via contacts with the capsid (CA) domain of the Gag polyprotein Pr55gag. The immunosuppressant drug cyclosporin A and the nonimmunosuppressive cyclosporin A analog SDZ NIM 811 bind t...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.70.9.5751-5757.1996

    authors： Dorfman T,Göttlinger HG

    更新日期：1996-09-01 00:00:00

  • Suppression of Leishmania RNA virus replication by capsid protein overexpression.

    abstract：:Some strains of the protozoan parasite genus Leishmania are persistently infected with single-segmented double-stranded RNA viruses, which are termed LRV. The function of these cytoplasmic viruses is unknown. In order to address the question of whether LRV affects the parasite's phenotype, pairs of isogenic LRV(+)-LRV...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.69.7.4122-4126.1995

    authors： Widmer G

    更新日期：1995-07-01 00:00:00

  • The Epstein-Barr virus replication protein BBLF2/3 provides an origin-tethering function through interaction with the zinc finger DNA binding protein ZBRK1 and the KAP-1 corepressor.

    abstract：:Herpesviruses encode a set of core proteins essential for lytic replication of their genomes. Three of these proteins form a tripartite helix-primase complex that, in the case of Epstein-Barr virus (EBV), consists of the helicase BBLF4, the primase BSLF1, and the linker protein BBLF2/3. BBLF2/3 and its homologs in the...

    journal_title：Journal of virology

    pub_type： 杂志文章

    doi：10.1128/JVI.79.1.245-256.2005

    authors： Liao G,Huang J,Fixman ED,Hayward SD

    更新日期：2005-01-01 00:00:00