Abstract:
:Hepatitis C virus (HCV) infection is believed to begin with interactions between cell-free HCV and cell receptors that include CD81, scavenger receptor B1 (SR-B1), claudin-1 (CLDN1), and occludin (OCLN). In this study, we have demonstrated that HCV spreading from infected hepatocytes to uninfected hepatocytes leads to the transfer of HCV and the formation of infection foci and is cell density dependent. This cell-cell contact-mediated (CCCM) HCV transfer occurs readily and requires all these known HCV receptors and an intact actin cytoskeleton. With a fluorescently labeled replication-competent HCV system, the CCCM transfer process was further dissected by live-cell imaging into four steps: donor cell-target cell contact, formation of viral puncta-target cell conjugation, transfer of viral puncta, and posttransfer. Importantly, the CCCM HCV transfer leads to productive infection of target cells. Taken together, these results show that CCCM HCV transfer constitutes an important and effective route for HCV infection and dissemination. These findings will aid in the development of new and novel strategies for preventing and treating HCV infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Liu Z,He JJdoi
10.1128/JVI.01062-13subject
Has Abstractpub_date
2013-08-01 00:00:00pages
8545-58issue
15eissn
0022-538Xissn
1098-5514pii
JVI.01062-13journal_volume
87pub_type
杂志文章abstract::Three amber mutations were introduced proximal to the syn3 locus of the herpes simplex virus type 1 glycoprotein B (gB) gene specifying gB derivatives lacking the carboxy-terminal 28, 49, or 64 amino acids. A complementation system that utilized gBs expressed in COS cells to complement gB-null virus K delta T was esta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2396-2401.1993
更新日期:1993-04-01 00:00:00
abstract::Merkel cell polyomavirus (MCPyV) is the major cause for Merkel cell carcinoma (MCC), a rare but highly aggressive skin cancer predominantly found in elderly and immunosuppressed patients. The early viral gene products large T-antigen (LT) and small T-antigen (sT) are important for efficient viral DNA replication, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01638-19
更新日期:2020-02-14 00:00:00
abstract::RNA viruses of filamentous fungi fall into two broad categories, those that contain double-stranded RNA (dsRNA) genomes in rigid particles and those that are more closely related to positive-sense, single-stranded RNA viruses with dsRNA replicative intermediates found within lipid vesicles. Effective infectivity syste...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.892-898.2004
更新日期:2004-01-01 00:00:00
abstract::We constructed vaccine vectors based on live recombinant vesicular stomatitis virus (VSV) and a Semliki Forest virus (SFV) replicon (SFVG) that propagates through expression of the VSV glycoprotein (G). These vectors expressing simian immunodeficiency virus (SIV) Gag and Env proteins were used to vaccinate rhesus maca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00342-11
更新日期:2011-06-01 00:00:00
abstract::In Epstein-Barr virus (EBV)-carrying nonproducer Raji cells, the induction of the viral replicative cycle by chemical treatment is limited to only the early stage and viral DNA synthesis is totally inhibited. We previously showed the absence of two messenger RNAs that are encoded by the BamHI-A fragment of the EBV gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7309-7314.1995
更新日期:1995-11-01 00:00:00
abstract::The global eradication of poliomyelitis will require substantial changes in immunization practices. One of the proposed scenarios includes cessation of vaccination with live oral poliovirus vaccine (OPV) and the creation of an OPV stockpile for emergency response in case of the reintroduction of poliovirus into circul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12460-12465.2003
更新日期:2003-12-01 00:00:00
abstract::Despite being able to use the Bonzo coreceptor as efficiently as CCR5 in transfected cells, pediatric human immunodeficiency virus type 1 isolate P6 was unable to replicate in peripheral blood mononuclear cells (PBMC) lacking the CCR5 receptor. Furthermore, its replication in wild-type PBMC was completely inhibited by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3443-3448.1999
更新日期:1999-04-01 00:00:00
abstract::We analyzed the susceptibility of 10 AKXD recombinant inbred (RI) mouse strains to lymphomas. These strains were derived from crosses of AKR/J, a highly lymphomatous strain, and DBA/2J, a weakly lymphomatous strain. Of the 10 strains analyzed, nine showed a high incidence of lymphoma development. As with the other 13 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2083-2090.1993
更新日期:1993-04-01 00:00:00
abstract:UNLABELLED:To investigate the molecular mechanism(s) by which herpes simplex virus 1 (HSV-1) tegument protein UL51 promotes viral replication, we screened for viral proteins that interact with UL51 in infected cells. Affinity purification of tagged UL51 in HSV-1-infected Vero cells was coupled with immunoblotting of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01258-16
更新日期:2016-09-12 00:00:00
abstract::To study the function of the varicella-zoster virus (VZV) gB cytoplasmic domain during viral infection, we produced a VZV recombinant virus that expresses a truncated form of gB lacking the C-terminal 36 amino acids of its cytoplasmic domain (VZV gB-36). VZV gB-36 replicates in noncomplementing cells and grows at a ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.2.591-599.2002
更新日期:2002-01-01 00:00:00
abstract::The use of endocytic pathways by viral glycoproteins is thought to play various functions during viral infection. We previously showed in transfection assays that herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) is transported from the cell surface back to the trans-Golgi network (TGN) and that two motifs of gB...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01231-07
更新日期:2007-12-01 00:00:00
abstract::Nucleotide sequencing and phylogenetic analysis of 10 recognized prototype strains of the porcine enterovirus (PEV) cytopathic effect (CPE) group I reveals a close relationship of the viral genomes to the previously sequenced strain F65, supporting the concept of a reclassification of this virus group into a new picor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1620-1631.2001
更新日期:2001-02-01 00:00:00
abstract::Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiological agent involved in the disease HTLV-1-associated myelopathy, or tropical spastic paraparesis (HAM/TSP). The pathogenesis of HAM/TSP is poorly understood, but it is probable that viral infection has an indirect, deleterious effect on neural function. In ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8727-8736.1996
更新日期:1996-12-01 00:00:00
abstract::The nature and theory of flow field-flow fractionation is described, and its potential applicability to virus-like particles is discussed. Different virus types are shown to be retained at different levels. Retention can be controlled by variation of the experimental parameters, in good agreement with theory. However,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.131-138.1977
更新日期:1977-01-01 00:00:00
abstract::Replication of poliovirus (PV) is restricted to a few sites, including the brain and spinal cord. However, this neurotropism is not conserved in cultured cells. Monkey kidney cells become susceptible to PV infection after cultivation in vitro, and cell lines of monolayer cultures from almost any tissue of primates are...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.9.4313-4325.2006
更新日期:2006-05-01 00:00:00
abstract::Adeno-associated virus (AAV) integrates very efficiently into a specific site (AAVS1) of human chromosome 19. Two elements of the AAV genome are sufficient: the inverted terminal repeats (ITRs) and the Rep78 or Rep68 protein. The incorporation of the AAV integration machinery in nonviral delivery systems is of great i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7653-7658.1998
更新日期:1998-09-01 00:00:00
abstract::Fischer rat fibroblasts transformed by polyoma virus contain, in addition to viral sequences integrated into the host genome, nonintegrated viral DNA molecules, whose presence is under the control of the viral A gene. To understand the mechanism of production of the "free" viral DNA, we have characterized the DNA spec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.3.615-626.1980
更新日期:1980-06-01 00:00:00
abstract::An in vitro system for production of hepatitis B virus (HBV) was established by infection of human hepatoma (HepG2) cells. HBV particles obtained from the serum of a chronic hepatitis B surface antigen (subtype ad) carrier were used to inoculate HepG2 cells. HBV envelope and core proteins were synthesized de novo by t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3025-3032.1990
更新日期:1990-06-01 00:00:00
abstract::To analyze the proposed growth-inhibitory function of wild-type p53, we compared simian virus 40 (SV40) DNA replication in primary rhesus monkey kidney (PRK) cells, which express wild-type p53, and in the established rhesus monkey kidney cell line LLC-MK2, which expresses a mutated p53 that does not complex with large...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.886-893.1993
更新日期:1993-02-01 00:00:00
abstract::Infectious pancreatic necrosis virus (IPNV) is a bisegmented, double-stranded RNA (dsRNA) virus of the Birnaviridae family that causes widespread disease in salmonids. Its two genomic segments are encapsulated together with the viral RNA-dependent RNA polymerase, VP1, and the assumed internal protein, VP3, in a single...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02831-06
更新日期:2007-06-01 00:00:00
abstract::Ten bacteriophages that attack and lyse saprophytic strains of Bdellovibrio bacteriovorus were isolated. Morphological, serological, and host-range studies revealed that there were four different bdellovibrio phages present among the isolates. One of the phages lysed a strain of B. bacteriovorus that requires the pres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.3.516-523.1972
更新日期:1972-09-01 00:00:00
abstract::A murine retrovirus encoding the middle T protein of polyomavirus infected and transformed nonestablished chicken embryo cells. The infected cultures formed colonies in soft agar-containing medium and released infectious transforming virus. Middle T protein expressed in the transformed chicken cells associated with p6...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.3.1023-1026.1985
更新日期:1985-12-01 00:00:00
abstract::Members of the Flaviviridae encode a serine proteinase termed NS3 that is responsible for processing at several sites in the viral polyproteins. In this report, we show that the NS3 proteinase of the pestivirus bovine viral diarrhea virus (BVDV) (NADL strain) is required for processing at nonstructural (NS) protein si...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5312-5322.1997
更新日期:1997-07-01 00:00:00
abstract::Beyond their role in cellular RNA metabolism, DExD/H-box RNA helicases are hijacked by various RNA viruses in order to assist replication of the viral genome. Here, we identify the DExH-box RNA helicase 9 (DHX9) as a binding partner of chikungunya virus (CHIKV) nsP3 mainly interacting with the C-terminal hypervariable...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01764-18
更新日期:2019-02-05 00:00:00
abstract::Serial serum specimens from 22 herpes simplex virus (HSV)-seronegative recipients of an HSV type 2 (HSV-2) glycoprotein subunit vaccine were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis for the development of antibodies to HSV-2 gB, gD, and g80, a complex of gC and gE. Volunteers receive...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.475-481.1985
更新日期:1985-11-01 00:00:00
abstract::The degree of genetic heterogeneity of DNA virus populations in nature and its consequences for disease control are virtually unknown. The parvovirus minute virus of mice (MVMi) was used here to investigate (i) the frequency of antibody-escape mutants in populations of a DNA virus and (ii) the ability of a DNA virus t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2701-2708.2003
更新日期:2003-02-01 00:00:00
abstract::Glycans on human immunodeficiency virus (HIV) envelope protein play an important role in infection and evasion from host immune responses. To examine the role of specific glycans, we introduced single or multiple mutations into potential N-linked glycosylation sites in hypervariable regions (V1 to V3) of the env gene ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01691-07
更新日期:2008-01-01 00:00:00
abstract::The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a possible influence of these proteins on replication and pathogenesis and about the mechanism of virus formation could not...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.4008-4021.2002
更新日期:2002-04-01 00:00:00
abstract:UNLABELLED:Lifelong antiretroviral therapy (ART) for HIV-1 does not diminish the established latent reservoir. A possible cure approach is to reactivate the quiescent genome from latency and utilize immune responses to eliminate cells harboring reactivated HIV-1. It is not known whether antibodies within HIV-1-infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02717-15
更新日期:2015-12-09 00:00:00
abstract::The avian sarcoma and leukosis virus (ASLV) family of retroviruses contains five highly related envelope subgroups (A to E) thought to have evolved from a common viral ancestor in the chicken population. Three genetic loci in chickens determine the susceptibility or resistance of cells to infection by the subgroup A t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02206-07
更新日期:2008-03-01 00:00:00