Two distinct sequence elements mediate retroviral gene expression in embryonal carcinoma cells.

abstract：:To replicate its segmented, double-stranded RNA (dsRNA) genome, the rotavirus RNA-dependent RNA polymerase, VP1, must recognize viral plus-strand RNAs (+RNAs) and guide them into the catalytic center. VP1 binds to the conserved 3' end of rotavirus +RNAs via both sequence-dependent and sequence-independent contacts. Se...

journal_title：Journal of virology

authors： Ogden KM,Ramanathan HN,Patton JT

更新日期：2011-03-01 00:00:00

abstract：:Genetic studies with immunocompetent mice show the importance of both T cells and gamma interferon (IFN-γ) for survival of a measles virus (MV) challenge; however, the direct role of T cells and IFN-γ within the MV-infected brain has not been addressed. Organotypic brain explants represent a successful ex vivo system ...

journal_title：Journal of virology

authors： Stubblefield Park SR,Widness M,Levine AD,Patterson CE

更新日期：2011-04-01 00:00:00

abstract：:Several reports have shown that human cytomegalovirus (HCMV)-infected cells are resistant to NK lysis. These studies have focused on receptor-ligand interactions, and different HCMV proteins have been indicated to mediate inhibitory NK signals. Here, we report that the HCMV protein UL16 is of major importance for the ...

journal_title：Journal of virology

authors： Odeberg J,Browne H,Metkar S,Froelich CJ,Brandén L,Cosman D,Söderberg-Nauclér C

更新日期：2003-04-01 00:00:00

abstract：:Extrachromosomal DNA purified from mink cells acutely infected with the Snyder-Theilen strain of feline sarcoma virus (FeSV) was digested with restriction endonucleases, and the DNA fragments were electrophoretically separated, transferred to a solid substrate, and hybridized with radiolabeled DNA transcripts compleme...

journal_title：Journal of virology

authors： Sherr CJ,Fedele LA,Donner L,Turek LP

更新日期：1979-12-01 00:00:00

abstract：:Human cytomegalovirus encodes four unique short (US) region proteins, each of which is independently sufficient for causing the down-regulation of major histocompatibility complex (MHC) class I molecules on the cell surface. This down-regulation enables infected cells to evade recognition by cytotoxic T lymphocytes (C...

journal_title：Journal of virology

authors： Shin J,Park B,Lee S,Kim Y,Biegalke BJ,Kang S,Ahn K

更新日期：2006-06-01 00:00:00

abstract：:Cell lines were generated by cotransfection of Vero cells with pSV2neo and a plasmid containing the herpes simplex virus type 1 (HSV-1) EcoRI D fragment (coordinates 0.086 to 0.194). One such cell line (S22) contained the genes for alkaline exonuclease and several uncharacterized functions. Three mutant isolates of HS...

journal_title：Journal of virology

authors： Carmichael EP,Kosovsky MJ,Weller SK

更新日期：1988-01-01 00:00:00

abstract：UNLABELLED:Nonhuman primates (NHPs) are a historically important source of zoonotic viruses and are a gold-standard model for research on many human pathogens. However, with the exception of simian immunodeficiency virus (SIV) (family Retroviridae), the blood-borne viruses harbored by these animals in the wild remain i...

journal_title：Journal of virology

authors： Bailey AL,Lauck M,Ghai RR,Nelson CW,Heimbruch K,Hughes AL,Goldberg TL,Kuhn JH,Jasinska AJ,Freimer NB,Apetrei C,O'Connor DH

更新日期：2016-07-11 00:00:00

abstract：:To examine the prerequisites for cleavage activation of the hemagglutinin of human influenza viruses, a cDNA clone obtained from strain A/Port Chalmers/1/73 (serotype H3) was subjected to site-directed mutagenesis and expressed in CV-1 cells by using a simian virus 40 vector. The number of basic residues at the cleava...

journal_title：Journal of virology

authors： Ohuchi R,Ohuchi M,Garten W,Klenk HD

更新日期：1991-07-01 00:00:00

abstract：:A 4,500-bp BamHI fragment, located within the HindIII A segment of the vaccinia virus genome, was found to contain eight potential coding regions for polypeptides of 78 to 346 amino acids. The open reading frames with 133, 346, and 125 codons were homologous to profilin (an actin-binding protein), 3-beta-hydroxysteroi...

journal_title：Journal of virology

authors： Blasco R,Cole NB,Moss B

更新日期：1991-09-01 00:00:00

abstract：:Beta interferon (IFN-beta) expression is triggered by double-stranded RNA, a common intermediate in the replication of many viruses including hepatitis C virus (HCV). The recent development of cell culture-grown HCV allowed us to analyze the IFN signaling pathway following virus infection. In this study, we have exami...

journal_title：Journal of virology

authors： Kanda T,Steele R,Ray R,Ray RB

更新日期：2007-11-01 00:00:00

abstract：:RNA was extracted from the Burkitt lymphoma-derived cell line Raji and from Burkitt lymphoma tumor biopsies, isotope labeled in vitro by iodination with 125I, and hybridized to electrophoretically separated restriction endonuclease fragments of Epstein-Barr virus DNA on nitrocellulose membranes. The results indicated ...

journal_title：Journal of virology

authors： Rymo L

更新日期：1979-10-01 00:00:00

abstract：:To identify essential host genes affecting replication of Tomato bushy stunt virus (TBSV), a small model plant virus, we screened 800 yeast genes present in the yeast Tet promoters Hughes Collection. In total, we have identified 30 new host genes whose down-regulation either increased or decreased the accumulation of ...

journal_title：Journal of virology

authors： Jiang Y,Serviene E,Gal J,Panavas T,Nagy PD

更新日期：2006-08-01 00:00:00

abstract：:Activation of microglia and astroglia is seen in many neurodegenerative diseases including prion diseases. Activated glial cells produce cytokines as a protective response against certain pathogens and as part of the host inflammatory response to brain damage. In addition, cytokines might also exacerbate tissue damage...

journal_title：Journal of virology

authors： Tribouillard-Tanvier D,Striebel JF,Peterson KE,Chesebro B

更新日期：2009-11-01 00:00:00

abstract：:The pathogenic parvovirus B19 (B19V) has an extreme tropism for human erythroid progenitor cells. In vitro, only a few erythroid leukemic cell lines (JK-1 and KU812Ep6) or megakaryoblastoid cell lines (UT7/Epo and UT7/Epo-S1) with erythroid characteristics support B19V replication, but these cells are only semipermiss...

journal_title：Journal of virology

authors： Wong S,Zhi N,Filippone C,Keyvanfar K,Kajigaya S,Brown KE,Young NS

更新日期：2008-03-01 00:00:00

abstract：:It has been reported that gene expression directed by the long terminal repeat of Rous sarcoma virus (RSV) is trans activated by a protein encoded in an alternate reading frame within the RSV gag gene (S. Broome and W. Gilbert, Cell 40:537-546, 1985). We have made specific mutations to test the role of the putative tr...

journal_title：Journal of virology

authors： Carlberg K,Beemon K

更新日期：1988-11-01 00:00:00

abstract：:Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mi...

journal_title：Journal of virology

authors： Fritz RB

更新日期：1974-01-01 00:00:00

abstract：:Animal influenza viruses pose a clear threat to public health. Transmissibility among humans is a prerequisite for a novel influenza virus to cause a human pandemic. A novel reassortant swine influenza virus acquired sustained human-to-human transmissibility and caused the 2009 influenza pandemic. However, the molecul...

journal_title：Journal of virology

authors： Zhang Y,Zhang Q,Gao Y,He X,Kong H,Jiang Y,Guan Y,Xia X,Shu Y,Kawaoka Y,Bu Z,Chen H

更新日期：2012-09-01 00:00:00

abstract：:The herpes simplex virus 1 infected cell protein 22 (ICP22), the product of the alpha22 gene, is a nucleotidylylated and phosphorylated nuclear protein with properties of a transcriptional factor required for the expression of a subset of viral genes. Here, we report the following. (i) ICP22 interacts with a previousl...

journal_title：Journal of virology

authors： Bruni R,Roizman B

更新日期：1998-11-01 00:00:00

abstract：:The relative antigenicity of the individual herpes simplex virus type 1 (KOS) glycoproteins gC and gB was analyzed in BALB/c mice by using KOS mutants altered in their ability to present these antigens on cell surface membranes during infection. The mutants employed were as follows: syn LD70 , a non-temperature-sensit...

journal_title：Journal of virology

authors： Glorioso J,Schröder CH,Kumel G,Szczesiul M,Levine M

更新日期：1984-06-01 00:00:00

abstract：:Arenaviruses can cause lethal hemorrhagic fevers in humans with few preventative and therapeutic measures. The arenaviral glycoprotein stable signal peptide (SSP) is unique among signal peptides in that it is an integral component of the mature glycoprotein complex (GPC) and plays important roles not only in GPC expre...

journal_title：Journal of virology

authors： Shao J,Liu X,Ly H,Liang Y

更新日期：2016-10-28 00:00:00

abstract：:Several members of the APOBEC3 DNA cytosine deaminase family can potently inhibit Vif-deficient human immunodeficiency virus type 1 (HIV-1) by catalyzing cytosine deamination in viral cDNA and impeding reverse transcription. HIV-1 counteracts restriction with the virally encoded Vif protein, which targets relevant APO...

journal_title：Journal of virology

authors： Wang J,Shaban NM,Land AM,Brown WL,Harris RS

更新日期：2018-05-29 00:00:00

abstract：:Extracts from nodavirus-infected Drosophila cells contained detergent-labile 140S "young" particles much richer than mature virions in their content of protein alpha, a precursor of coat proteins beta and gamma. Incorporation studies in infected cells showed that most newly synthesized alpha protein was assembled into...

journal_title：Journal of virology

authors： Gallagher TM,Rueckert RR

更新日期：1988-09-01 00:00:00

abstract：:A sequence of polyadenylic acid, homogeneous in composition but heterogeneous in length, was isolated from complete pancreatic RNase digests of both middle and bottom RNAs of cowpea mosaic virus. The polyadenylic acid was 3'-terminal and occurred once per molecule. A fragment consisting of the polyadenylic acid and ap...

journal_title：Journal of virology

authors： Steele KP,Frist RH

更新日期：1978-05-01 00:00:00

abstract：:A lysate from hypotonically swollen polyoma-infected BALB/3T3 cells incorporated labeled deoxynucleotide triphosphates into both viral and cellular DNAs. The incorporation was stimulated by the presence of ATP, deoxynucleotide triphosphates, thiols, and magnesium ions. Strong inhibition of incorporation was observed w...

journal_title：Journal of virology

authors： Hunter T,Francke B

更新日期：1974-01-01 00:00:00

abstract：:The Kilham strain of polyomavirus (KV) infects vascular endothelial cells in vivo (J. E. Greenlee, Infect. Immun. 26:705-713, 1979), but no permissive cell type for growth of the virus in vitro has been identified. The failure of KV DNA to replicate in mouse fibroblast cells after transfection suggested that viral gen...

journal_title：Journal of virology

authors： Zhang S,Magnusson G

更新日期：2001-11-01 00:00:00

abstract：:To further define the transcriptional regulation of the P38 promoter in the minute virus of mice (MVM) genome, we constructed a series of internal deletion and linker scanning mutations. The mutant P38 constructs were assayed for transcriptional activity in vitro by primer extension analysis with nuclear extracts from...

journal_title：Journal of virology

authors： Ahn JK,Pitluk ZW,Ward DC

更新日期：1992-06-01 00:00:00

abstract：:Nonhuman primates are widely used to study correlates of protective immunity in AIDS research. Successful cellular immune responses have been difficult to identify because heterogeneity within macaque major histocompatibility complex (MHC) genes results in quantitative and qualitative differences in immune responses. ...

journal_title：Journal of virology

authors： Wiseman RW,Wojcechowskyj JA,Greene JM,Blasky AJ,Gopon T,Soma T,Friedrich TC,O'Connor SL,O'Connor DH

更新日期：2007-01-01 00:00:00

abstract：:WUH4 is a highly pathogenic North American porcine reproductive and respiratory syndrome virus (PRRSV). Unlike previous PRRSV isolates, which were mainly recovered from sera or tissues, WUH4 was isolated from a piglet stool sample. Here we announce its complete genome sequence. ...

journal_title：Journal of virology

authors： Song T,Fang L,Zeng S,Li B,Chen H,Xiao S

更新日期：2012-04-01 00:00:00

abstract：:The nucleoprotein of the WSN strain of influenza was found to be phosphorylated in vitro. The phosphate-protein bond was stable to hot trichloroacetic acid, RNase, DNase, succinic acid, and succinic acid-hydroxylamine, but sensitive to hydrolysis by bacterial alkaline phosphatase. This suggested that the nucleoprotein...

journal_title：Journal of virology

authors： Privalsky ML,Penhoet EE

更新日期：1977-10-01 00:00:00

abstract：:Drug susceptibility and mutations in the reverse transcriptase (RT) gene were analyzed with 167 virus isolates from 38 patients treated with nevirapine, a potent nonnucleoside inhibitor of human immunodeficiency virus type 1 (HIV-1) RT. Resistant isolates emerged quickly and uniformly in all patients administered nevi...

journal_title：Journal of virology

authors： Richman DD,Havlir D,Corbeil J,Looney D,Ignacio C,Spector SA,Sullivan J,Cheeseman S,Barringer K,Pauletti D

更新日期：1994-03-01 00:00:00