Structural analysis of determinants of histo-blood group antigen binding specificity in genogroup I noroviruses.


UNLABELLED:Human noroviruses (NoVs) cause acute epidemic gastroenteritis. Susceptibility to the majority of NoV infections is determined by genetically controlled secretor-dependent expression of histo-blood group antigens (HBGAs), which are also critical for NoV attachment to host cells. Human NoVs are classified into two major genogroups (genogroup I [GI] and GII), with each genogroup further divided into several genotypes. GII NoVs are more prevalent and exhibit periodic emergence of new variants, suggested to be driven by altered HBGA binding specificities and antigenic drift. Recent epidemiological studies show increased activity among GI NoVs, with some members showing the ability to bind nonsecretor HBGAs. NoVs bind HBGAs through the protruding (P) domain of the major capsid protein VP1. GI NoVs, similar to GII, exhibit significant sequence variations in the P domain; it is unclear how these variations affect HBGA binding specificities. To understand the determinants of possible strain-specific HBGA binding among GI NoVs, we determined the structure of the P domain of a GI.7 clinical isolate and compared it to the previously determined P domain structures of GI.1 and GI.2 strains. Our crystallographic studies revealed significant structural differences, particularly in the loop regions of the GI.7 P domain, altering its surface topography and electrostatic landscape and potentially indicating antigenic variation. The GI.7 strain bound to H- and A-type, Lewis secretor, and Lewis nonsecretor families of HBGAs, allowing us to further elucidate the structural determinants of nonsecretor HBGA binding among GI NoVs and to infer several contrasting and generalizable features of HBGA binding in the GI NoVs. IMPORTANCE:Human noroviruses (NoVs) cause acute epidemic gastroenteritis. Recent epidemiological studies have shown increased prevalence of genogroup I (GI) NoVs. Although secretor-positive status is strongly correlated with NoV infection, cases of NoV infection associated with secretor-negative individuals are reported. Biochemical studies have shown that GI NoVs exhibit genotype-dependent binding to nonsecretor histo-blood group antigens (HBGAs). From our crystallographic studies of a GI.7 NoV, in comparison with previous studies on GI.1 and GI.2 NoVs, we show that genotypic differences translate to extensive structural changes in the loop regions that significantly alter the surface topography and electrostatic landscape of the P domain; these features may be indicative of antigenic variations contributing to serotypic differentiation in GI NoVs and also differential modulation of the HBGA binding characteristics. A significant finding is that the threshold length and the structure of one of the loops are critical determinants in the binding of GI NoVs to nonsecretor HBGAs.


J Virol


Journal of virology


Shanker S,Czako R,Sankaran B,Atmar RL,Estes MK,Prasad BV




Has Abstract


2014-06-01 00:00:00














  • Selection of revertants of Kirsten sarcoma virus transformed nonproducer BALB-3T3 cells.

    abstract::Revertants of Kirsten sarcoma virus transformed nonproducer BALB/3T3 cells (KA31 cells) were isolated after exposing the transformed cells to 5-fluorodeoxyuridine at high cell density, or when suspended in methylcellulose. Revertants were also isolated by treating KA31 cells with the lectin, concanavalin A, which is m...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ozanne B,Vogel A

    更新日期:1974-08-01 00:00:00

  • Functional characterization of Xenopus laevis p53: evidence of temperature-sensitive transactivation but not of repression.

    abstract::We have investigated the effect of Xenopus laevis p53 (Xp53) on transcription from a variety of promoters which are regulated by mouse p53 using a chloramphenicol acetyltransferase reporter system. Although Xp53 transactivated promoters that are up-regulated by mouse p53, it was unable to cause repression. This abilit...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ridgway PJ,Soussi T,Braithwaite AW

    更新日期:1994-11-01 00:00:00

  • Hepatitis B virus X protein interacts with a probable cellular DNA repair protein.

    abstract::The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lee TH,Elledge SJ,Butel JS

    更新日期:1995-02-01 00:00:00

  • Porcine Reproductive and Respiratory Syndrome Virus Structural Protein GP3 Regulates Claudin 4 To Facilitate the Early Stages of Infection.

    abstract::Claudins (CLDN) are a family of proteins that represent the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between epithelial cells. Several types of viruses make full use of CLDN to facilitate entry into cells....

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ding G,Liu J,Shao Q,Wang B,Feng J,Li Y,Li L,Cao S,Cong F,Zhao Y,Liu S,Xiao Y

    更新日期:2020-09-29 00:00:00

  • Clade-specific differences between human immunodeficiency virus type 1 clades B and C: diversity and correlations in C3-V4 regions of gp120.

    abstract::Current knowledge of human immunodeficiency virus type 1 envelope (Env) glycoprotein structure and function is based on studies of clade B viruses. We present evidence of sequence and structural differences in viral glycoprotein gp120 between clades B and C. In clade C, the C3 region alpha2-helix exhibits high sequenc...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gnanakaran S,Lang D,Daniels M,Bhattacharya T,Derdeyn CA,Korber B

    更新日期:2007-05-01 00:00:00

  • In vitro construction of different oligomeric forms of lambdadv DNA and studies of their transforming activities.

    abstract::Plasmid lambdadv1, which is in a dimeric form, was converted to a linear monomer duplex by the action of EcoRI restriction endonuclease that incises at a unique site in this plasmid genome. The resulting products were then joined by Escherichia coli DNA ligase to produce molecules with various oligomeric forms, and fr...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Matsubara K,Takagi Y,Mukai T

    更新日期:1975-09-01 00:00:00

  • Identification of a lytic-phase origin of DNA replication in human herpesvirus 6B strain Z29.

    abstract::DNA sequences which have structural features suggestive of their functioning as an origin of lytic-phase DNA replication were previously identified in both human herpesvirus 6B strain Z29 [HHV-6B (Z29)] and in HHV-6A (U1102). Plasmid constructs containing the putative HHV-6B (Z29) oriLyt element were replicated after ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Dewhurst S,Dollard SC,Pellett PE,Dambaugh TR

    更新日期:1993-12-01 00:00:00

  • B-lymphoma induction by reticuloendotheliosis virus: characterization of a mutated chicken syncytial virus provirus involved in c-myc activation.

    abstract::Nondefective reticuloendotheliosis virus induces chicken bursal lymphoma in a manner similar to that of avian leukosis virus. The provirus integrates in the c-myc locus and uses a promoter insertion mechanism to activate c-myc expression. We cloned a provirus involved in c-myc activation from a B lymphoma. Detailed st...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Swift RA,Boerkoel C,Ridgway A,Fujita DJ,Dodgson JB,Kung HJ

    更新日期:1987-07-01 00:00:00

  • Early bone marrow hematopoietic defect in simian/human immunodeficiency virus C2/1-infected macaques and relevance to advance of disease.

    abstract::To clarify hematological abnormalities following infection with human immunodeficiency virus (HIV), we examined the hematopoietic capability of bone marrow by using cynomolgus monkeys infected with pathogenic simian/human immunodeficiency virus (SHIV) strain C2/1, an animal model of HIV infection. The relationship bet...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yamakami K,Honda M,Takei M,Ami Y,Kitamura N,Nishinarita S,Sawada S,Horie T

    更新日期:2004-10-01 00:00:00

  • Unusual features of vaccinia virus extracellular virion form neutralization resistance revealed in human antibody responses to the smallpox vaccine.

    abstract::The extracellular virion form (EV) of vaccinia virus (VACV) is essential for viral pathogenesis and is difficult to neutralize with antibodies. Why this is the case and how the smallpox vaccine overcomes this challenge remain incompletely understood. We previously showed that high concentrations of anti-B5 antibodies ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Benhnia MR,Maybeno M,Blum D,Aguilar-Sino R,Matho M,Meng X,Head S,Felgner PL,Zajonc DM,Koriazova L,Kato S,Burton DR,Xiang Y,Crowe JE Jr,Peters B,Crotty S

    更新日期:2013-02-01 00:00:00

  • Free major histocompatibility complex class I heavy chain is preferentially targeted for degradation by human T-cell leukemia/lymphotropic virus type 1 p12(I) protein.

    abstract::Human T-cell leukemia virus type 1 (HTLV-1) establishes a persistent infection in the host despite a vigorous virus-specific immune response. Here we demonstrate that an HTLV-1-encoded protein, p12(I), resides in the endoplasmic reticulum (ER) and Golgi and physically binds to the free human major histocompatibility c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Johnson JM,Nicot C,Fullen J,Ciminale V,Casareto L,Mulloy JC,Jacobson S,Franchini G

    更新日期:2001-07-01 00:00:00

  • Interaction of the human papillomavirus type 16 E6 oncoprotein with wild-type and mutant human p53 proteins.

    abstract::The E6 oncoproteins encoded by the cancer-associated human papillomaviruses (HPVs) can associate with and promote the degradation of wild-type p53 in vitro. To gain further insight into this process, the ability of HPV-16 E6 to complex with and promote the degradation of mutant forms of p53 was studied. A correlation ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Scheffner M,Takahashi T,Huibregtse JM,Minna JD,Howley PM

    更新日期:1992-08-01 00:00:00

  • Human cytomegalovirus infection alters the expression of cellular microRNA species that affect its replication.

    abstract::The human genome encodes over 500 microRNAs (miRNAs), small RNAs (19 to 26 nucleotides [nt]) that regulate the expressions of diverse cellular genes. Many cellular processes are altered through a variety of mechanisms by human cytomegalovirus (HCMV) infection. We asked whether HCMV infection leads to changes in the ex...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wang FZ,Weber F,Croce C,Liu CG,Liao X,Pellett PE

    更新日期:2008-09-01 00:00:00

  • Regulation of Sendai virus transcription: evidence for a single promoter in vivo.

    abstract::The synthesis of Sendai virus RNA species was examined after UV irradiation of cells late in infection. Compared with the inactivation of 50S genomic RNA synthesis, the synthesis of the group of mRNA species that sediments at 18S was inactivated at an average rate consistent with a process of sequential transcription ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Glazier K,Raghow R,Kingsbury DW

    更新日期:1977-03-01 00:00:00

  • Establishment of B-cell lines latently infected with reactivation-competent murine gammaherpesvirus 68 provides evidence for viral alteration of a DNA damage-signaling cascade.

    abstract::Gammaherpesvirus 68 (gammaHV68, or MHV68) is a naturally occurring rodent pathogen that replicates to high titer in cell culture and is amenable to in vivo experimental evaluation of viral and host determinants of gammaherpesvirus disease. However, the inability of MHV68 to transform primary murine B cells in culture,...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Forrest JC,Speck SH

    更新日期:2008-08-01 00:00:00

  • Isolation and characterization of a temperate bacteriophage specific for Rhodopseudomonas spheroides.

    abstract::The isolation of a temperature phage specific for the photosynthetic bacterium Rhodopseudomonas spheroides is reported. This phage, Rphi-1, establishes a state of lysogeny and can be induced from the prophage state by exposure to mitomycin C or UV irradiation. Mutants of Rphi-1 which grow on a standard laboratory stra...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mural RJ,Friedman DI

    更新日期:1974-11-01 00:00:00

  • Nucleotide sequence of the Akv env gene.

    abstract::The sequence of 2,191 nucleotides encoding the env gene of murine retrovirus Akv was determined by using a molecular clone of the Akv provirus. Deduction of the encoded amino acid sequence showed that a single open reading frame encodes a 638-amino acid precursor to gp70 and p15E. In addition, there is a typical leade...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lenz J,Crowther R,Straceski A,Haseltine W

    更新日期:1982-05-01 00:00:00

  • Sequence requirements for Sindbis virus subgenomic mRNA promoter function in cultured cells.

    abstract::The Sindbis virus minimal subgenomic mRNA promoter (spanning positions -19 to +5 relative to the subgenomic mRNA start site) is approximately three- to sixfold less active than the fully active -98 to +14 promoter region. We identified two elements flanking the -19 to +5 region which increase its transcription to leve...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wielgosz MM,Raju R,Huang HV

    更新日期:2001-04-01 00:00:00

  • Dynamic regulation of host restriction factor expression over the course of HIV-1 infection in vivo.

    abstract::In this study, we investigated the expression levels of host restriction factors in six untreated HIV-1-positive patients over the course of infection. We found that the host restriction factor gene expression profile consistently increased over time and was significantly associated with CD4+ T cell activation and vir...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Raposo RA,Abdel-Mohsen M,Deng X,Hecht FM,Pilcher CD,Pillai SK,Nixon DF

    更新日期:2014-10-01 00:00:00

  • Roles of the sequence encoding tobacco etch virus capsid protein in genome amplification: requirements for the translation process and a cis-active element.

    abstract::The roles of the capsid protein (CP) and the CP coding sequence of tobacco etch potyvirus (TEV) in genome amplification were analyzed. A series of frameshift-stop codon mutations that interrupted translation of the CP coding sequence at various positions were introduced into the TEV genome. A series of 3' deletion mut...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mahajan S,Dolja VV,Carrington JC

    更新日期:1996-07-01 00:00:00

  • Efficient infectious cell culture systems of the hepatitis C virus (HCV) prototype strains HCV-1 and H77.

    abstract:UNLABELLED:The first discovered and sequenced hepatitis C virus (HCV) genome and the first in vivo infectious HCV clones originated from the HCV prototype strains HCV-1 and H77, respectively, both widely used in research of this important human pathogen. In the present study, we developed efficient infectious cell cult...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Li YP,Ramirez S,Mikkelsen L,Bukh J

    更新日期:2015-01-01 00:00:00

  • Replication of ONYX-015, a potential anticancer adenovirus, is independent of p53 status in tumor cells.

    abstract::The 55-kDa E1B protein of adenovirus, which binds to and inactivates the tumor suppressor protein p53, is not expressed in the adenoviral mutant termed ONYX-015 (i.e., dl1520). It was reported that the mutant virus due to a deletion in E1B is able to replicate only in cells deficient for wild-type p53. Accordingly, dl...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rothmann T,Hengstermann A,Whitaker NJ,Scheffner M,zur Hausen H

    更新日期:1998-12-01 00:00:00

  • DNA vaccine molecular adjuvants SP-D-BAFF and SP-D-APRIL enhance anti-gp120 immune response and increase HIV-1 neutralizing antibody titers.

    abstract:UNLABELLED:Broadly neutralizing antibodies (bNAbs) specific for conserved epitopes on the HIV-1 envelope (Env) are believed to be essential for protection against multiple HIV-1 clades. However, vaccines capable of stimulating the production of bNAbs remain a major challenge. Given that polyreactivity and autoreactivit...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gupta S,Clark ES,Termini JM,Boucher J,Kanagavelu S,LeBranche CC,Abraham S,Montefiori DC,Khan WN,Stone GW

    更新日期:2015-04-01 00:00:00

  • Phosphorylation of NS protein by vesicular stomatitis virus nucleocapsids: lack of effect during RNA synthesis and separation of kinase from L protein.

    abstract::The relationship between NS protein phosphorylation and RNA polymerase activities was determined in nucleocapsids purified from vesicular stomatitis virus grown in BHK cells. Phosphate incorporation into endogenous NS protein under transcription conditions reached a maximum value of 0.06 mol/mol of NS within 20 to 30 ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Massey DM,Deans N,Lenard J

    更新日期:1990-07-01 00:00:00

  • Impairment of human immunodeficiency virus type 1 (HIV-1) entry into Jurkat T cells by constitutive expression of the HIV-1 vpr protein: role of CD4 down-modulation.

    abstract::Jurkat T-cell clones, stably expressing the human immunodeficiency virus type 1 (HIV-1) Vpr protein, exhibited an impaired susceptibility to HIV-1 infection. A marked down-modulation of surface CD4 receptors was detected in Vpr-expressing clones with respect to control cells. Likewise, a reduced CD4 expression was als...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Conti L,Varano B,Gauzzi MC,Matarrese P,Federico M,Malorni W,Belardelli F,Gessani S

    更新日期:2000-11-01 00:00:00

  • Combinations of two capsid regions controlling canine host range determine canine transferrin receptor binding by canine and feline parvoviruses.

    abstract::Feline panleukopenia virus (FPV) and its host range variant, canine parvovirus (CPV), can bind the feline transferrin receptor (TfR), while only CPV binds to the canine TfR. Introducing two CPV-specific changes into FPV (at VP2 residues 93 and 323) endowed that virus with the canine TfR binding property and allowed ca...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hueffer K,Govindasamy L,Agbandje-McKenna M,Parrish CR

    更新日期:2003-09-01 00:00:00

  • Role of immunoproteasome catalytic subunits in the immune response to hepatitis B virus.

    abstract::Inhibition of hepatitis B virus (HBV) replication and viral clearance from an infected host requires both the innate and adaptive immune responses. Expression of interferon (IFN)-inducible proteasome catalytic and regulatory subunits correlates with the IFN-alpha/beta- and IFN-gamma-mediated noncytopathic inhibition o...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Robek MD,Garcia ML,Boyd BS,Chisari FV

    更新日期:2007-01-01 00:00:00

  • The central conserved cystine noose of the attachment G protein of human respiratory syncytial virus is not required for efficient viral infection in vitro or in vivo.

    abstract::The G glycoprotein of human respiratory syncytial virus (RSV) was identified previously as the viral attachment protein. Although we and others recently showed that G is not essential for replication in vitro, it does affect the efficiency of replication in a cell type-dependent fashion and is required for efficient r...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Teng MN,Collins PL

    更新日期:2002-06-01 00:00:00

  • Severe acute respiratory syndrome coronavirus nsp1 suppresses host gene expression, including that of type I interferon, in infected cells.

    abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) nsp1 protein has unique biological functions that have not been described in the viral proteins of any RNA viruses; expressed SARS-CoV nsp1 protein has been found to suppress host gene expression by promoting host mRNA degradation and inhibiting translation....

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Narayanan K,Huang C,Lokugamage K,Kamitani W,Ikegami T,Tseng CT,Makino S

    更新日期:2008-05-01 00:00:00

  • Intrauterine transmission of human T-cell leukemia virus type I in rats.

    abstract::To analyze intrauterine transmission, MT-2 cells, a human T-cell line producing human T-cell leukemia virus type I (HTLV-I), were injected into eight pregnant F344 rats, and cesarean section was performed at day 23 of pregnancy. HTLV-I provirus was detected by PCR in the liver and spleen taken from one of the eight fe...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hori M,Ami Y,Kushida S,Kobayashi M,Uchida K,Abe T,Miwa M

    更新日期:1995-02-01 00:00:00