Effects of an epitope-specific CD8+ T-cell response on murine coronavirus central nervous system disease: protection from virus replication and antigen spread and selection of epitope escape mutants.


:Both CD4(+) and CD8(+) T cells are required for clearance of the murine coronavirus mouse hepatitis virus (MHV) during acute infection. We investigated the effects of an epitope-specific CD8(+) T-cell response on acute infection of MHV, strain A59, in the murine CNS. Mice with CD8(+) T cells specific for gp33-41 (an H-2D(b)-restricted CD8(+) T-cell epitope derived from lymphocytic choriomeningitis glycoprotein) were infected with a recombinant MHV-A59, also expressing gp33-41, as a fusion protein with enhanced green fluorescent protein (EGFP). By 5 days postinfection, these mice showed significantly (approximately 20-fold) lower titers of infectious virus in the brain compared to control mice. Furthermore mice with gp33-41-specific CD8(+) cells exhibited much reduced levels of viral antigen in the brain as measured by immunohistochemistry using an antibody directed against viral nucleocapsid. More than 90% of the viruses recovered from brain lysates of such protected mice, at 5 days postinfection, had lost the ability to express EGFP and had deletions in their genomes encompassing EGFP and gp33-41. In addition, genomes of viruses from about half the plaques that retained the EGFP gene had mutations within the gp33-41 epitope. On the other hand, gp33-41-specific cells failed to protect perforin-deficient mice from infection by the recombinant MHV expressing gp33, indicating that perforin-mediated mechanisms were needed. Virus recovered from perforin-deficient mice did not exhibit loss of EGFP expression and the gp33-41 epitope. These observations suggest that the cytotoxic T-cell response to gp33-41 exerts a strong immune pressure that quickly selects epitope escape mutants to gp33-41.


J Virol


Journal of virology


Chua MM,MacNamara KC,San Mateo L,Shen H,Weiss SR




Has Abstract


2004-02-01 00:00:00












  • Sequence conservation and antigenic variation of the structural proteins of equine rhinitis A virus.

    abstract::The nucleotide and deduced amino acid sequences of the P1 region of the genomes of 10 independent equine rhinitis A virus (ERAV) isolates were determined and found to be very closely related. A panel of seven monoclonal antibodies to the prototype virus ERAV.393/76 that bound to nonneutralization epitopes conserved am...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Varrasso A,Drummer HE,Huang JA,Stevenson RA,Ficorilli N,Studdert MJ,Hartley CA

    更新日期:2001-11-01 00:00:00

  • Protease digestion of hepatitis A virus: disparate effects on capsid proteins, antigenicity, and infectivity.

    abstract::High concentrations of either trypsin or chymotrypsin caused nearly complete cleavage of capsid protein VP2 of hepatitis A virus but did not significantly reduce the infectivity, thermostability, or antigenicity of the virus. Chymotrypsin also had a lesser effect on VP1. These findings indicate the presence of a prote...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lemon SM,Amphlett E,Sangar D

    更新日期:1991-10-01 00:00:00

  • Regeneration of herpesviruses from molecularly cloned subgenomic fragments.

    abstract::The ability to manipulate the genomes of herpesviruses is of eminent importance for obtaining insight into gene function and regulation of gene expression of these complex viruses. Here we report the use of in vivo overlap recombination to generate pseudorabies virus mutants. Cotransfection of up to five overlapping c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: van Zijl M,Quint W,Briaire J,de Rover T,Gielkens A,Berns A

    更新日期:1988-06-01 00:00:00

  • Separation of human immunodeficiency virus type 1 replication from nef-mediated pathogenesis in the human thymus.

    abstract::Human immunodeficiency virus type 1 (HIV-1) is frequently attenuated after long-term culture in vitro. The attenuation process probably involves mutations of functions required for replication and pathogenicity in vivo. Analysis of attenuated HIV-1 for replication and pathogenicity in vivo will help to define these fu...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Duus KM,Miller ED,Smith JA,Kovalev GI,Su L

    更新日期:2001-04-01 00:00:00

  • The Mlvi-1 locus involved in the induction of rat T-cell lymphomas and the pvt-1/Mis-1 locus are identical.

    abstract::Mlvi-1 defines a locus of proviral integration in rat thymomas induced by Moloney murine leukemia virus. pvt-1/Mis-1 represents an independently identified locus which becomes rearranged either by chromosomal translocation in murine plasmacytomas or by provirus insertion in retrovirus-induced murine and rat thymic lym...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Koehne CF,Lazo PA,Alves K,Lee JS,Tsichlis PN,O'Donnell PV

    更新日期:1989-05-01 00:00:00

  • Palmitoylated Cysteines in Chikungunya Virus nsP1 Are Critical for Targeting to Cholesterol-Rich Plasma Membrane Microdomains with Functional Consequences for Viral Genome Replication.

    abstract::In mammalian cells, alphavirus replication complexes are anchored to the plasma membrane. This interaction with lipid bilayers is mediated through the viral methyl/guanylyltransferase nsP1 and reinforced by palmitoylation of cysteine residue(s) in the C-terminal region of this protein. Lipid content of membranes suppo...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bakhache W,Neyret A,Bernard E,Merits A,Briant L

    更新日期:2020-05-04 00:00:00

  • The cellular interactome of the coronavirus infectious bronchitis virus nucleocapsid protein and functional implications for virus biology.

    abstract::The coronavirus nucleocapsid (N) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. These functions are likely to be facilitated by interactions with host cell proteins. The potential interactome of t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Emmott E,Munday D,Bickerton E,Britton P,Rodgers MA,Whitehouse A,Zhou EM,Hiscox JA

    更新日期:2013-09-01 00:00:00

  • Virus factories of cauliflower mosaic virus are virion reservoirs that engage actively in vector transmission.

    abstract::Cauliflower mosaic virus (CaMV) forms two types of inclusion bodies within infected plant cells: numerous virus factories, which are the sites for viral replication and virion assembly, and a single transmission body (TB), which is specialized for virus transmission by aphid vectors. The TB reacts within seconds to ap...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bak A,Gargani D,Macia JL,Malouvet E,Vernerey MS,Blanc S,Drucker M

    更新日期:2013-11-01 00:00:00

  • Inhibition of viral RNA methylation in herpes simplex virus type 1-infected cells by 5' S-isobutyl-adenosine.

    abstract::5' S-isobutyl-adenosine (SIBA), a structural analogue of S-adenosylhomocysteine, reversibly blocks the multiplication of herpes simplex type 1 virus. In the presence of SIBA, viral protein synthesis is inhibited. After removing SIBA the synthesis of proteins starts rapidly again. The new polypeptides are mainly alpha ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Jacquemont B,Huppert J

    更新日期:1977-04-01 00:00:00

  • The C Protein Is Recruited to Measles Virus Ribonucleocapsids by the Phosphoprotein.

    abstract::Measles virus (MeV), like all viruses of the order Mononegavirales, utilizes a complex consisting of genomic RNA, nucleoprotein, the RNA-dependent RNA polymerase, and a polymerase cofactor, the phosphoprotein (P), for transcription and replication. We previously showed that a recombinant MeV that does not express anot...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pfaller CK,Bloyet LM,Donohue RC,Huff AL,Bartemes WP,Yousaf I,Urzua E,Clavière M,Zachary M,de Masson d'Autume V,Carson S,Schieferecke AJ,Meyer AJ,Gerlier D,Cattaneo R

    更新日期:2020-01-31 00:00:00

  • Inhibition of host translation in encephalomyocarditis virus-infected L cells: a novel mechanism.

    abstract::Encephalomyocarditis virus induced a rapid shutoff of host translation in mouse L cells shortly after infection and before viral proteins were made in detectable amounts. This kinetic pattern is similar to that seen in poliovirus-infected HeLa cells. However, the mechanisms of host shutoff are different in these two c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Jen G,Thach RE

    更新日期:1982-07-01 00:00:00

  • Hantaan virus infection of human endothelial cells.

    abstract::The primary pathophysiologic finding of the viral disease known as Korean hemorrhagic fever, the etiological agent of which is Hantaan virus (HTV), is vascular instability. To investigate whether HTV was able to infect cells derived from human vascular tissue and alter their behavior, we infected in vitro primary adul...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pensiero MN,Sharefkin JB,Dieffenbach CW,Hay J

    更新日期:1992-10-01 00:00:00

  • Release of genotype 1 hepatitis E virus from cultured hepatoma and polarized intestinal cells depends on open reading frame 3 protein and requires an intact PXXP motif.

    abstract::Hepatitis E virus genotype 1 strain Sar55 replicated in subcloned Caco-2 intestinal cells and Huh7 hepatoma cells that had been transfected with in vitro transcribed viral genomes, and hepatitis E virions were released into the culture medium of both cell lines. Virus egress from cells depended on open reading frame 3...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Emerson SU,Nguyen HT,Torian U,Burke D,Engle R,Purcell RH

    更新日期:2010-09-01 00:00:00

  • Purification and characterization of the major nonstructural protein (NS-1) of Aleutian mink disease parvovirus.

    abstract::We have previously described the expression of the major nonstructural protein (NS-1) of Aleutian mink disease parvovirus (ADV) in insect cells by using a baculovirus vector (J. Christensen, T. Storgaard, B. Bloch, S. Alexandersen, and B. Aasted, J. Virol. 67:229-238, 1993). To study its biochemical properties, ADV NS...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Christensen J,Pedersen M,Aasted B,Alexandersen S

    更新日期:1995-03-01 00:00:00

  • Site-specific cleavage of the host poly(A) binding protein by the encephalomyocarditis virus 3C proteinase stimulates viral replication.

    abstract::Although picornavirus RNA genomes contain a 3'-terminal poly(A) tract that is critical for their replication, the impact of encephalomyocarditis virus (EMCV) infection on the host poly(A)-binding protein (PABP) remains unknown. Here, we establish that EMCV infection stimulates site-specific PABP proteolysis, resulting...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kobayashi M,Arias C,Garabedian A,Palmenberg AC,Mohr I

    更新日期:2012-10-01 00:00:00

  • Herpes simplex virus trans-regulatory protein ICP27 stabilizes and binds to 3' ends of labile mRNA.

    abstract::Previous work demonstrated that a herpes simplex virus type 1 (HSV-1) immediate-early function up-regulates beta interferon but not chloramphenicol acetyltransferase reporter genes driven by the strong simian virus 40 (SV40) or cytomegalovirus promoter-enhancer regions in both transient assays and stable cell lines. T...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Brown CR,Nakamura MS,Mosca JD,Hayward GS,Straus SE,Perera LP

    更新日期:1995-11-01 00:00:00

  • Mechanism of Cell Culture Adaptation of an Enteric Calicivirus, the Porcine Sapovirus Cowden Strain.

    abstract:UNLABELLED:The porcine sapovirus (SaV) (PoSaV) Cowden strain is one of only a few culturable enteric caliciviruses. Compared to the wild-type (WT) PoSaV Cowden strain, tissue culture-adapted (TC) PoSaV has two conserved amino acid substitutions in the RNA-dependent RNA polymerase (RdRp) and six in the capsid protein (V...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lu Z,Yokoyama M,Chen N,Oka T,Jung K,Chang KO,Annamalai T,Wang Q,Saif LJ

    更新日期:2015-11-18 00:00:00

  • Relative contributions of measles virus hemagglutinin- and fusion protein-specific serum antibodies to virus neutralization.

    abstract::The relative contribution of measles virus hemagglutinin (H)- or fusion protein (F)-specific antibodies to virus neutralization (VN) has not been demonstrated. We have depleted these specific antibodies from sera collected from young adults, who had been vaccinated during childhood, by prolonged incubation with viable...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: de Swart RL,Yüksel S,Osterhaus AD

    更新日期:2005-09-01 00:00:00

  • Role of a single amino acid at the amino terminus of the simian virus 5 F2 subunit in syncytium formation.

    abstract::The fusion (F) protein of simian virus 5 (strain W3A) induced extensive cell fusion in BHK cells when expressed alone, while that of strain WR did not. Mutational analysis demonstrated that the fusing activity can be transferred to the WR F protein by a proline residue at position 22 of subunit W3A F2. ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ito M,Nishio M,Kawano M,Kusagawa S,Komada H,Ito Y,Tsurudome M

    更新日期:1997-12-01 00:00:00

  • Structure of the ribonucleoprotein of influenza virus.

    abstract::The ribonucleoprotein (RNP) internal components of influenza virus were separated into distinct size classes by sedimentation in glycerol gradients and examined by electron microscopy by using positive staining with uranyl acetate. The large RNP have a peak in length distribution at 90 to 110 nm, the medium, at 60 to ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Compans RW,Content J,Duesberg PH

    更新日期:1972-10-01 00:00:00

  • Evolution of feline leukemia virus variant genomes with insertions, deletions, and defective envelope genes in infected cats with tumors.

    abstract::In order to study retroviral variation, selection, and viral correlates of in vivo pathogenicity, we documented the evolution of feline leukemia virus (FeLV) variants in cats that died with thymic lymphoma after infection with molecularly cloned subgroup A FeLV. Using genomic DNA from cat necropsy samples, we employed...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rohn JL,Linenberger ML,Hoover EA,Overbaugh J

    更新日期:1994-04-01 00:00:00

  • Liquefaction of semen generates and later degrades a conserved semenogelin peptide that enhances HIV infection.

    abstract:UNLABELLED:Semen enhances HIV infection in vitro, but how long it retains this activity has not been carefully examined. Immediately postejaculation, semen exists as a semisolid coagulum, which then converts to a more liquid form in a process termed liquefaction. We demonstrate that early during liquefaction, semen exh...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Roan NR,Liu H,Usmani SM,Neidleman J,Müller JA,Avila-Herrera A,Gawanbacht A,Zirafi O,Chu S,Dong M,Kumar ST,Smith JF,Pollard KS,Fändrich M,Kirchhoff F,Münch J,Witkowska HE,Greene WC

    更新日期:2014-07-01 00:00:00

  • Attenuated murine cytomegalovirus binds to N-acetylglucosamine, and shift to virulence may involve recognition of sialic acids.

    abstract::Treatment of cells with lectins specific for N-acetylglucosamine (GlcNAc) blocked infection by mouse cytomegalovirus (MCMV), and GlcNAc pretreatment of the lectin blocked this effect. MCMV failed to infect N-acetylglucosaminidase (GlcNAcase)-treated mouse embryo fibroblasts (MEF). GlcNAc and GlcNAc-containing syntheti...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ravindranath RM,Graves MC

    更新日期:1990-11-01 00:00:00

  • Bone marrow chimeras reveal non-H-2 hematopoietic control of susceptibility to Theiler's virus persistent infection.

    abstract::The DA strain of Theiler's murine encephalomyelitis virus persists in the white matter of the spinal cords of susceptible mice. Previous results showed that the difference in susceptibility to viral persistence between the susceptible SJL/J strain and the resistant B10.S strain was due to multiple non-H-2 loci. The re...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Aubagnac S,Brahic M,Bureau JF

    更新日期:2002-06-01 00:00:00

  • Two major outer envelope glycoproteins of Epstein-Barr virus are encoded by the same gene.

    abstract::Two major outer envelope glycoproteins of Epstein-Barr virus, gp350 and gp220, are known to be encoded by 3.2- and 2.5-kilobase RNAs which map to the same DNA fragment (M. Hummel, D. Thorley-Lawson, and E. Kieff, J. Virol. 49:413-417). These RNAs have the same 5' and 3' ends. The larger RNA is encoded by a 2,777-base ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Beisel C,Tanner J,Matsuo T,Thorley-Lawson D,Kezdy F,Kieff E

    更新日期:1985-06-01 00:00:00

  • Rearrangement of a common cellular DNA domain on chromosome 4 in human primary liver tumors.

    abstract::Hepatitis B virus (HBV) DNA integration has been shown to occur frequently in human hepatocellular carcinomas. We have investigated whether common cellular DNA domains might be rearranged, possibly by HBV integration, in human primary liver tumors. Unique cellular DNA sequences adjacent to an HBV integration site were...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pasquinelli C,Garreau F,Bougueleret L,Cariani E,Grzeschik KH,Thiers V,Croissant O,Hadchouel M,Tiollais P,Bréchot C

    更新日期:1988-02-01 00:00:00

  • Molecular cloning and phylogenetic analysis of human immunodeficiency virus type 1 subtype C: a set of 23 full-length clones from Botswana.

    abstract::To better understand the virological aspect of the expanding AIDS epidemic in southern Africa, a set of 23 near-full-length clones of human immunodeficiency virus type 1 (HIV-1) representing eight AIDS patients from Botswana were sequenced and analyzed phylogenetically. All study viruses from Botswana belonged to HIV-...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Novitsky VA,Montano MA,McLane MF,Renjifo B,Vannberg F,Foley BT,Ndung'u TP,Rahman M,Makhema MJ,Marlink R,Essex M

    更新日期:1999-05-01 00:00:00

  • Analysis of structural proteins of purified murine cytomegalovirus.

    abstract::Murine cytomegalovirus propagated in mouse embryo fibroblasts was purified by the following procedures. (i) Extracellular virus was concentrated by centrifugation at 100,000 x g for 90 min. (ii) The concentrated virus was passed through a Bio-Rad Bio-Gel A-15m column to eliminate contaminating materials smaller than 1...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kim KS,Sapienza VJ,Carp RI,Moon HM

    更新日期:1976-03-01 00:00:00

  • Characterization of the pneumococcal bacteriophage HB-3 amidase: cloning and expression in Escherichia coli.

    abstract::HB-3, a temperate bacteriophage of Streptococcus pneumoniae, synthesizes its own murein hydrolase activity when multiplying on cultures of pneumococcus. The enzyme (HBL) was purified and biochemically characterized as an N-acetylmuramoyl-L-alanine amidase of 36,000 daltons, and a 2.1-kilobase DraI fragment containing ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Romero A,Lopez R,Garcia P

    更新日期:1990-01-01 00:00:00

  • Broadly neutralizing antibodies targeted to mucin-type carbohydrate epitopes of human immunodeficiency virus.

    abstract::The cancer-related mucin-type carbohydrate neoantigen Tn was found on gp160 and gp120 of human immunodeficiency virus (HIV). Immunoglobulin G (IgG) and IgM monoclonal antibodies (MAbs) against Tn neutralized infection with cell-free virus and blocked fusion between HIV-infected and uninfected cells. This inhibition wa...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hansen JE,Nielsen C,Arendrup M,Olofsson S,Mathiesen L,Nielsen JO,Clausen H

    更新日期:1991-12-01 00:00:00