Abstract:
:Human cytomegalovirus (HCMV) infection of monocyte-derived macrophages (MDM) results in delayed and nonlytic productive viral growth. During late stages of replication, infectious virus remains cell associated in cytoplasmic vacuoles. In order to understand HCMV survival and persistence in MDM, we examined mechanisms involved in the formation and trafficking of HCMV-containing vacuoles in these cells. Utilizing double-label immunofluorescence with antibodies to viral and cellular proteins, HCMV-containing vacuoles were associated with the Golgi apparatus marker mannosidase II but not with markers to early endosomes (transferrin receptor and rab5) or late endosomes and early lysosomes (LAMP-1 and -2). In addition, as late-stage viral infection progressed in MDM, the cells displayed increasing abnormalities in the Golgi apparatus. Analysis of structural features of infected cells revealed the disruption of the microtubule network. These observations suggest a novel mechanism by which HCMV is vacuolized in MDM, avoiding degradation and release from the cell.
journal_name
J Viroljournal_title
Journal of virologyauthors
Fish KN,Britt W,Nelson JAdoi
10.1128/JVI.70.3.1855-1862.1996subject
Has Abstractpub_date
1996-03-01 00:00:00pages
1855-62issue
3eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::Transcription of the terminal protein (TP) gene of Epstein-Barr virus (EBV) in Burkitt's lymphoma cells, in EBV-negative Burkitt's lymphoma cells converted with transformation-defective (P3HR1) and transformation-competent (B95-8, AG876) EBV strains, and in EBV-immortalized cell lines was studied. A TP1 cDNA probe spa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.415-423.1991
更新日期:1991-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5391-5398.1997
更新日期:1997-07-01 00:00:00
abstract::Shrimp subcuticular epithelial cells are the initial and major targets of white spot syndrome virus (WSSV) infection. Proteomic studies of WSSV-infected subcuticular epithelium of Penaeus monodon were performed through two approaches, namely, subcellular fractionation coupled with shotgun proteomics to identify viral ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01006-07
更新日期:2007-11-01 00:00:00
abstract::To understand the roles of heptad repeat 1(HR1) and HR2 of the spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) in virus-cell interactions, the conserved Leu or Ile residues located at positions 913, 927, 941, and 955 in HR1 and 1151, 1165, and 1179 in HR2 were individually replaced wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3225-3237.2006
更新日期:2006-04-01 00:00:00
abstract::Human bocavirus 1 (HBoV1) is a human parvovirus that causes acute respiratory tract infections in young children. In this study, we confirmed that, when polarized/well-differentiated human airway epithelia are infected with HBoV1 in vitro, they develop damage characterized by barrier function disruption and cell hypot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01533-17
更新日期:2017-11-30 00:00:00
abstract::Adenovirus infection and expression of E1A induces both proliferation and apoptosis, the latter of which is blocked by the adenovirus Bcl-2 homologue E1B 19K. The mechanism of apoptosis induction and the role that it plays in productive infection are not known. Unlike apoptosis mediated by death receptors, infection w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4547-4558.2002
更新日期:2002-05-01 00:00:00
abstract::Dendritic cells are central to the early events of human immunodeficiency virus type 1 (HIV-1) transmission, but HIV-1 infects dendritic cells inefficiently in vitro compared to activated CD4(+) T cells. There is a strong postentry restriction of HIV-1 infection in dendritic cells, partly mediated by the cellular rest...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00800-07
更新日期:2007-11-01 00:00:00
abstract::Hepadnaviruses contain a relaxed circular DNA genome (RC-DNA) with discontinuities in both strands. Upon infectious entry into a host cell, this genome is converted into a covalently closed superhelical form (CCC-DNA), which later serves as the template for transcription. Here we examined whether the viral polymerase ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4867-4874.1993
更新日期:1993-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2047-2056.1990
更新日期:1990-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.746-749.1997
更新日期:1997-01-01 00:00:00
abstract::Incubation of highly purified adeno-associated virus (AAV) capsids in vitro at pH 5.5 induced significant autocleavage of capsid proteins at several amino acid positions. No autocleavage was seen at pH 7.5. Examination of other AAV serotypes showed at least two different pH-induced cleavage patterns, suggesting that d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01717-12
更新日期:2012-11-01 00:00:00
abstract::As nonacademic careers in science have become less and less "alternative," one field that has consistently attracted early-career virologists is public health research. The desire to make tangible contributions to public health needs and better protect the public from infectious disease often motivates the transition....
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01416-16
更新日期:2017-04-13 00:00:00
abstract::Endogenous retroviruses, records of past retroviral infections, are ubiquitous in vertebrate genomes. On occasion, vertebrate hosts have co-opted retroviral genes for their own biological functions. Here, we perform a phylogenomic survey of retroviral gag gene homologs within vertebrate genomes and identify two ancien...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00542-19
更新日期:2019-06-28 00:00:00
abstract::Defective interfering particles (DIPs) of equine herpesvirus 1 (EHV-1; Kentucky A strain) mediate persistent infection. DNA sequences at the L terminus, which contain the UL2 gene (homolog of UL55 of herpes simplex virus type 1 and open reading frame 3 of varicella-zoster virus) of standard EHV-1, have been shown to b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2255-2265.1993
更新日期:1993-04-01 00:00:00
abstract::Equine herpesvirus 1 (EHV1) replicates in the respiratory epithelium and disseminates through the body via a cell-associated viremia in leukocytes, despite the presence of neutralizing antibodies. "Hijacked" leukocytes, previously identified as monocytic cells and T lymphocytes, transmit EHV1 to endothelial cells of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02098-18
更新日期:2019-03-21 00:00:00
abstract::Bank vole is a rodent species that shows differential susceptibility to the experimental transmission of different prion strains. In this work, the transmission features of a panel of diverse prions with distinct origins were assayed both in bank vole expressing methionine at codon 109 (Bv109M) and in transgenic mice ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01592-16
更新日期:2016-11-14 00:00:00
abstract::The widespread presence of endogenous retroviruses in the genomes of animals and humans has suggested that these viruses may be involved in both normal and abnormal developmental processes. Previous studies have indicated the involvement of endogenous ecotropic murine leukemia virus (MuLV) in the development of age-de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.10.4362-4369.1989
更新日期:1989-10-01 00:00:00
abstract::We have previously shown that delivery of the porcine type I interferon gene (poIFN-α/β) with a replication-defective human adenovirus vector (adenovirus 5 [Ad5]) can sterilely protect swine challenged with foot-and-mouth disease virus (FMDV) 1 day later. However, the need of relatively high doses of Ad5 limits the ap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03462-12
更新日期:2013-05-01 00:00:00
abstract::A lysate from hypotonically swollen polyoma-infected BALB/3T3 cells incorporated labeled deoxynucleotide triphosphates into both viral and cellular DNAs. The incorporation was stimulated by the presence of ATP, deoxynucleotide triphosphates, thiols, and magnesium ions. Strong inhibition of incorporation was observed w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.125-139.1974
更新日期:1974-01-01 00:00:00
abstract::HLA-B*52:01 is strongly associated with protection against HIV disease progression. However, the mechanisms of HLA-B*52:01-mediated immune control have not been well studied. We here describe a cohort with a majority of HIV C-clade-infected individuals from Delhi, India, where HLA-B*52:01 is highly prevalent (phenotyp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02025-19
更新日期:2020-06-16 00:00:00
abstract::Expression of the immediate-early (IE) genes of herpes simplex virus (HSV) is specifically stimulated by a 65-kilodalton virion transcription factor (VF65 or VP16) that is introduced as a component of infecting virions. In both the IE175(ICP4) and IE110(ICP0) promoters, this activation requires an upstream cis-acting ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2798-2812.1989
更新日期:1989-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.926-932.1976
更新日期:1976-06-01 00:00:00
abstract::The Kilham strain of polyomavirus (KV) infects vascular endothelial cells in vivo (J. E. Greenlee, Infect. Immun. 26:705-713, 1979), but no permissive cell type for growth of the virus in vitro has been identified. The failure of KV DNA to replicate in mouse fibroblast cells after transfection suggested that viral gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10015-10023.2001
更新日期:2001-11-01 00:00:00
abstract::Beta interferon (IFN-beta) expression is triggered by double-stranded RNA, a common intermediate in the replication of many viruses including hepatitis C virus (HCV). The recent development of cell culture-grown HCV allowed us to analyze the IFN signaling pathway following virus infection. In this study, we have exami...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01695-07
更新日期:2007-11-01 00:00:00
abstract::Mutations at amino acids 143, 148, and 155 in HIV-1 integrase (IN) define primary resistance pathways in subjects failing raltegravir (RAL)-containing treatments. Although each pathway appears to be genetically distinct, shifts in the predominant resistant virus population have been reported under continued drug press...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06618-11
更新日期:2012-07-01 00:00:00
abstract::In an effort to identify host proteins involved in herpes simplex virus type 1 replication, monkey and human cellular protein activities (called OF-1) that bind the viral replication origin, oriS, have been described. We show by mass spectrometry that the DNA-binding component of human OF-1 contains Ku70 and Ku80 prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7839-7842.2004
更新日期:2004-07-01 00:00:00
abstract::The smallest capsid proteins (SCPs) of the human herpesviruses differ substantially in size and sequence and are thought to impart some unique aspects of infection to their respective viruses. We used electron cryomicroscopy and antibody labeling to show that the 8-kDa SCP of human cytomegalovirus is attached only to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1327-1332.2005
更新日期:2005-01-01 00:00:00
abstract::During paramyxovirus entry into a host cell, receptor engagement by a specialized binding protein triggers conformational changes in the adjacent fusion protein (F), leading to fusion between the viral and cell membranes. According to the existing paradigm of paramyxovirus membrane fusion, the initial activation of F ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05873-11
更新日期:2011-12-01 00:00:00
abstract::Epstein-Barr virus (EBV) uses tonsils as the portal of entry to establish persistent infection. EBV is found in various B-cell subsets in tonsils but exclusively in memory B cells in peripheral blood. The in vitro susceptibilities of B-cell subsets to EBV infection have been studied solely qualitatively. In this work,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02630-07
更新日期:2008-05-01 00:00:00
abstract::We have previously demonstrated that the human papillomavirus (HPV) genome replicates effectively in U2OS cells after transfection using electroporation. The transient extrachromosomal replication, stable maintenance, and late amplification of the viral genome could be studied for high- and low-risk mucosal and cutane...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01943-12
更新日期:2013-01-01 00:00:00