Abstract:
:Hepadnaviruses contain a relaxed circular DNA genome (RC-DNA) with discontinuities in both strands. Upon infectious entry into a host cell, this genome is converted into a covalently closed superhelical form (CCC-DNA), which later serves as the template for transcription. Here we examined whether the viral polymerase activity is required for this repair reaction. Primary hepatocytes prepared from embryonated duck eggs were infected with the duck hepatitis B virus. Conversion of the RC-DNA into the CCC-DNA was then analyzed by a newly developed polymerase chain reaction technique. This method allows the efficient discrimination between the two DNA forms and is sensitive enough to monitor repair of the infecting viral DNA in the absence of replication and amplification. Thus, we were able to monitor this process in the presence of a potent inhibitor of the viral polymerase, the nucleoside analog 2',3'-dideoxyguanosine. The data show that inhibition of the viral polymerase activity has no influence on genome repair, suggesting that this enzymatic function is not required for conversion of the RC-DNA into the CCC-DNA. Consequently, antiviral drugs blocking the polymerase activity cannot prevent the infectious entry of the virus into a host cell.
journal_name
J Viroljournal_title
Journal of virologyauthors
Köck J,Schlicht HJdoi
10.1128/JVI.67.8.4867-4874.1993subject
Has Abstractpub_date
1993-08-01 00:00:00pages
4867-74issue
8eissn
0022-538Xissn
1098-5514journal_volume
67pub_type
杂志文章abstract::Naked DNA vaccines expressing the prM and E genes of two tick-borne flaviviruses, Russian spring summer encephalitis (RSSE) virus and Central European encephalitis (CEE) virus were evaluated in mice. The vaccines were administered by particle bombardment of DNA-coated gold beads by Accell gene gun inoculation. Two imm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9563-9569.1997
更新日期:1997-12-01 00:00:00
abstract::Hepatitis delta virus (HDV) is a defective RNA virus which is dependent on hepatitis B virus for essential helper functions. Only a single highly basic phosphoprotein, HDV antigen (HDAg), is expressed by the HDV genome during infection in humans. Antibody directed to HDAg is important in the diagnosis of HDV infection...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1108-1116.1990
更新日期:1990-03-01 00:00:00
abstract::Marburg virus (MARV) has been associated with sporadic episodes of hemorrhagic fever, including a recent highly publicized outbreak in Angola that produced severe disease and significant mortality in infected patients. MARV is also considered to have potential as a biological weapon. Recently, we reported the developm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00959-06
更新日期:2006-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5720-5728.2002
更新日期:2002-06-01 00:00:00
abstract::The retroviral Gag precursor plays an important role in the assembly of virion particles. The capsid (CA) protein of the Gag molecule makes a major contribution to this process. In the crystal structure of the free CA protein of the human immunodeficiency virus type 1 (HIV-1), 11 residues of the C terminus were found ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9675-9688.2004
更新日期:2004-09-01 00:00:00
abstract::Rabies, caused by rabies virus (RABV), is a fatal encephalitis in humans and other mammals, which continues to present a public health threat in most parts of the world. Our previous study demonstrated that Toll-like receptor 7 (TLR7) is essential in the induction of anti-RABV antibodies via the facilitation of germin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00111-20
更新日期:2020-04-16 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5619-5625.1998
更新日期:1998-07-01 00:00:00
abstract::Recruitment and extravasation of T cells through the blood-brain barrier are favored by adhesion molecule-mediated interactions of circulating T cells with endothelial cells. Since a common pathological finding in human T-cell leukemia virus type 1 (HTLV-1)-associated diseases is the infiltration of HTLV-1-infected T ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8522-8530.1997
更新日期:1997-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-18
更新日期:2019-01-17 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00139-16
更新日期:2016-07-11 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.6723-6731.2005
更新日期:2005-06-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1)-encoded RNA-binding protein Tat is known to play an essential role in viral gene expression. In the search for novel compounds to inhibit Tat transactivity, one coumarin derivative, BPRHIV001, was identified, with a 50% effective concentration (EC(50)) against HIV-1 at 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00175-11
更新日期:2011-09-01 00:00:00
abstract::The STP-C488 open reading frame of herpesvirus saimiri encodes an oncoprotein that has transforming and tumor-inducing activities independent of the rest of the herpesvirus genome. STP-C488 protein has an unusual, membrane-associated, fibrous structure and is located primarily in perinuclear compartments. We now repor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.3.1777-1780.1992
更新日期:1992-03-01 00:00:00
abstract::Nef is a regulatory gene product of human immunodeficiency virus type 1 (HIV-1) and other primate lentiviruses which enhances virion infectivity by an unknown mechanism. We report here that Nef is detectable at moderate levels in preparations of HIV-1 virions which lack active viral protease (PR). Significantly smalle...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1013-1018.1997
更新日期:1997-02-01 00:00:00
abstract::Under natural conditions and in some experimental models, rabies virus infection of the central nervous system causes relatively mild histopathological changes, without prominent evidence of neuronal death despite its lethality. In this study, the effects of rabies virus infection on the structure of neurons were inve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01677-07
更新日期:2008-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2522-2529.2003
更新日期:2003-02-01 00:00:00
abstract::Transfection of the E6 and E7 genes of the high-risk human papillomaviruses (HPVs) into primary genital keratinocytes generates colonies of proliferating cells which are resistant to calcium- and serum-induced terminal differentiation. To genetically map the HPV gene(s) responsible for this cellular phenotype, we clon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3269-3279.1996
更新日期:1996-05-01 00:00:00
abstract::Polypeptide synthesis has been studied in cell-free systems prepared from vaccinia virus-infected and uninfected HeLa cells. Cytoplasmic extracts containing endogenous messenger ribonucleic acid were used. Amino acid incorporation into hot trichloroacetic acid-precipitable material was linear for 15 to 20 min at 37 C....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.4.416-422.1969
更新日期:1969-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12333-12343.2004
更新日期:2004-11-01 00:00:00
abstract::Of the four required herpes simplex virus (HSV) entry glycoproteins, the precise role of gH-gL in fusion remains the most elusive. The heterodimer gH-gL has been proposed to mediate hemifusion after the interaction of another required glycoprotein, gD, with a receptor. To identify functional domains of HSV-1 gH, we ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02215-09
更新日期:2010-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00
abstract::The initial wave of swine-origin influenza A virus (pandemic H1N1/09) in the United States during the spring and summer of 2009 also resulted in an increased vigilance and sampling of seasonal influenza viruses (H1N1 and H3N2), even though they are normally characterized by very low incidence outside of the winter mon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00018-10
更新日期:2010-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02402-06
更新日期:2007-03-01 00:00:00
abstract::It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered reg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01781-15
更新日期:2015-11-01 00:00:00
abstract::Cytomegalovirus (CMV) infection is the most common opportunistic infection in immunosuppressed individuals, such as transplant recipients or people living with HIV/AIDS, and congenital CMV is the leading viral cause of developmental disabilities in infants. Due to the highly species-specific nature of CMV, animal mode...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05840-11
更新日期:2011-12-01 00:00:00
abstract:UNLABELLED:The ORF45 protein of Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus-specific immediate-early tegument protein. Our previous studies have revealed its crucial roles in both early and late stages of KSHV infection. In this study, we surveyed the interactome of ORF45 using a panel of monoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02925-14
更新日期:2015-05-01 00:00:00
abstract::A series of plasmids containing hepatitis A virus (HAV) cDNA was constructed such that positive-strand HAV RNA could be transcribed with T7 RNA polymerase. The plasmids differed in the number of 5'-terminal nucleotides representing the junctions between vectors and HAV sequences that were present in the transcripts. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2757-2760.1991
更新日期:1991-05-01 00:00:00
abstract::A number of retrovirus-like sequences have been cloned from chimpanzee DNA which constitute the chimpanzee homologs of the endogenous colobus type C virus CPC-1. One of the clones contains a nearly complete viral genome, but others have sustained deletions of 1 to 2 kilobases in the polymerase gene. The pattern of rel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.3.914-924.1982
更新日期:1982-09-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of PRRS, which has important impacts on the pig industry. PRRSV infection results in disruption of the swine leukocyte antigen class I (SLA-I) antigen presentation pathway. In this study, highly pathogenic PRRSV (HP-PRRSV) infection inh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01755-16
更新日期:2017-02-14 00:00:00
abstract::The pneumopathogenicity of a trypsin-sensitive revertant of Sendai virus, TSrev-58, which was derived from a trypsin-resistant mutant, TR-5, was examined in mice. In comparison with TR-5, the revertant had a single amino acid substitution at residue 116 (Ile----Arg) on F protein, which was the cleavage site, and had t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.3040-3042.1988
更新日期:1988-08-01 00:00:00