Abstract:
:A series of plasmids containing hepatitis A virus (HAV) cDNA was constructed such that positive-strand HAV RNA could be transcribed with T7 RNA polymerase. The plasmids differed in the number of 5'-terminal nucleotides representing the junctions between vectors and HAV sequences that were present in the transcripts. When these transcripts were used to transfect cultured BS-C-1 cells, it was found that only those transcripts that contained all of the 5'-terminal HAV nucleotides, in addition to one or more nucleotides from the vector, were capable of initiating an infectious cycle leading to production of progeny virus. Transcripts that contained one 5'-terminal nucleotide from the vector sequence but were missing two uridylate residues corresponding to the first two nucleotides of HAV sequences, or were missing U and C residues corresponding to nucleotides 2 and 3 of the HAV sequence, were not infectious. A similar plasmid containing poliovirus cDNA was engineered to produce transcripts similarly lacking the first two uridylate residues of the poliovirus RNA sequence. These transcripts were infectious.
journal_name
J Viroljournal_title
Journal of virologyauthors
Harmon SA,Richards OC,Summers DF,Ehrenfeld Edoi
10.1128/JVI.65.5.2757-2760.1991subject
Has Abstractpub_date
1991-05-01 00:00:00pages
2757-60issue
5eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
杂志文章abstract::Recombinant poxvirus vectors are undergoing intensive evaluation as vaccine candidates for a variety of infectious pathogens. Avipoxviruses, such as canarypox virus, are replication deficient in mammalian cells by virtue of a poorly understood species-specific restriction. Highly attenuated vaccinia virus strains such...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02654-06
更新日期:2007-07-01 00:00:00
abstract::Several arenaviruses, chiefly Lassa virus (LASV) and Junin virus in West Africa and Argentina, respectively, cause hemorrhagic fever (HF) disease in humans that is associated with high morbidity and significant mortality. The investigation of antiviral strategies to combat HF arenaviruses is hampered by the requiremen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02229-10
更新日期:2011-02-01 00:00:00
abstract::We performed live cell visualization assays to directly assess the interaction between competing adeno-associated virus (AAV) and herpes simplex virus type 1 (HSV-1) DNA replication. Our studies reveal the formation of separate AAV and HSV-1 replication compartments and the inhibition of HSV-1 replication compartment ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02476-06
更新日期:2007-05-01 00:00:00
abstract::The C-terminal domain of human immunodeficiency virus type 1 (HIV-1) integrase (IN) is a dimer that binds to DNA in a nonspecific manner. The structure of the minimal region required for DNA binding (IN220-270) has been solved by nuclear magnetic resonance spectroscopy. The overall fold of the C-terminal domain of HIV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4841-4848.1998
更新日期:1998-06-01 00:00:00
abstract::We studied the effect of filovirus infection on host cell gene expression by characterizing the regulation of gene expression responses in human liver cells infected with Zaire Ebolavirus (ZEBOV), Reston Ebolavirus (REBOV), and Marburgvirus (MARV), using transcriptional profiling and bioinformatics. Expression microar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.6.3009-3020.2006
更新日期:2006-03-01 00:00:00
abstract::A procedure incorporating the use of heparin was developed to purify Herpesvirus ovis. The viral DNA has a buoyant density of 1.706 +/- 0.001 g/cm3, and the sedimentation constant was estimated to be 47.5 +/- 1.5S; from the latter, the molecular weight was calculated as 67.3 +/- 5.4 X 10(6). Estimates of the guanine-p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.705-709.1979
更新日期:1979-12-01 00:00:00
abstract::Oncornavirus 60 to 70S ribonucleic acids (RNA), such as those from avian myeloblastosis virus, Schmidt-Ruppin virus, or mouse sarcoma-mouse leukemia viruses, isolated by conventional techniques, contain 4S transferlike RNA molecules that are released upon dissociation of the 60 to 70S RNA with heat. The 4S RNA represe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.2.254-256.1971
更新日期:1971-08-01 00:00:00
abstract::To analyze proteolytic processing of foamy (spuma) retroviruses, two mutations were generated in the presumed active-site triplet Asp-Ser-Gly in the predicted proteinase (PR) region of the human foamy virus (HSRV). The mutations changed either the presumed catalytic aspartic acid residue to a catalytically incompetent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7264-7268.1995
更新日期:1995-11-01 00:00:00
abstract::Human herpesvirus 8 (HHV8) open reading frame (ORF) 21 is predicted to encode a protein similar to the thymidine kinase (TK) enzyme of other herpesviruses. Expressed in mammalian cells, ORF 21 was found to have low TK activity, based on poor growth in media containing hypoxanthine-aminopterin-thymidine (HAT) and low i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.684-692.2000
更新日期:2000-01-01 00:00:00
abstract::Using a DNA probe from an avian transforming virus, NK24, that contains the fos gene and that was newly isolated and characterized (M. Nishizawa, N. Goto, and S. Kawai, J. Virol. 61:3733-3740, 1987), we cloned and sequenced the chicken c-fos gene. Results showed that this gene, like the mouse or human c-fos gene, cont...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4012-4018.1987
更新日期:1987-12-01 00:00:00
abstract::CD81 has been described as a putative receptor for hepatitis C virus (HCV); however, its role in HCV cell entry has not been characterized due to the lack of an efficient cell culture system. We have examined the role of CD81 in HCV glycoprotein-dependent entry by using a recently developed retroviral pseudotyping sys...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1448-1455.2004
更新日期:2004-02-01 00:00:00
abstract::The immune response to viral glycoproteins is often directed against conformation- and/or glycosylation-dependent structures; synthetic peptides and bacterially expressed proteins are inadequate probes for the mapping of such epitopes. This report describes a retroviral vector system that presents such native epitopes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.400-410.1994
更新日期:1994-01-01 00:00:00
abstract::Initiation of herpesvirus infection requires attachment of virions to the host cell followed by fusion of virion envelope and cellular cytoplasmic membrane during penetration. In several alphaherpesviruses, glycoprotein C (gC) is the primary attachment protein, interacting with cell-surface heparan sulfate proteoglyca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7341-7348.1998
更新日期:1998-09-01 00:00:00
abstract::Equine rhinitis A virus (ERAV) has recently been classified as an aphthovirus, a genus otherwise comprised of the different serotypes of Foot-and-mouth disease virus (FMDV). FMDV initiates translation via a type II internal ribosomal entry site (IRES) and utilizes two in-frame AUG codons to produce the leader proteina...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11708-11716.2000
更新日期:2000-12-01 00:00:00
abstract::The locations of replication pause sites in the simian virus 40 minichromosome which were determined by sizing cloned fragments of nascent DNA (Zannis-Hadjopoulos et al., J. Mol. Biol. 165:599-607, 1983) were compared with the positions of simian virus 40 nucleosomes in the genome, as obtained by sequence-directed map...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1011-1012.1984
更新日期:1984-12-01 00:00:00
abstract::Fibropapillomatosis (FP) of marine turtles is an emerging neoplastic disease associated with infection by a novel turtle herpesvirus, fibropapilloma-associated turtle herpesvirus (FPTHV). This report presents 23 kb of the genome of an FPTHV infecting a Hawaiian green turtle (Chelonia mydas). By sequence homology, the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1125-1132.2005
更新日期:2005-01-01 00:00:00
abstract::Antisera were prepared against the three structural polypeptides of HADEN virus dissociated with sodium dodecyl sulfate. These immunological reagents were used in immunofluorescence tests to study the kinetics and location of polypeptide antigen appearance in infected cells. These sera did not neutralize virus infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.608-613.1974
更新日期:1974-03-01 00:00:00
abstract::Marek's disease (MD), a highly infectious disease caused by an oncogenic herpesvirus, is one of the few herpesvirus diseases against which live attenuated vaccines are used as the main strategy for control. We have constructed bacterial artificial chromosomes (BACs) of the CVI988 (Rispens) strain of the virus, the mos...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.16.8712-8718.2003
更新日期:2003-08-01 00:00:00
abstract::In infected cells, hepatitis C virus (HCV) induces the formation of membrane alterations referred to as membranous webs, which are sites of RNA replication. In addition, HCV RNA replication also occurs in smaller membrane structures that are associated with the endoplasmic reticulum. However, cellular mechanisms invol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01190-09
更新日期:2010-01-01 00:00:00
abstract::Infection of cells with enveloped viruses is accomplished through membrane fusion. The binding and fusion processes are mediated by the spike proteins in the envelope of the virus particle and usually involve a series of conformational changes in these proteins. We have studied the low-pH-mediated fusion process of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7309-7318.1992
更新日期:1992-12-01 00:00:00
abstract::The vpr gene product of human immunodeficiency virus type (HIV-1) is a virion-associated regulatory protein. A transferable virion association motif for Vpr is located in the p6 domain of the HIV-1 Gag polyprotein. To map the sequences in p6 that are involved in Vpr incorporation, we analyzed the ability of mutant for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.159-164.1996
更新日期:1996-01-01 00:00:00
abstract:UNLABELLED:Numerous experimental studies have demonstrated that CD8(+) T cells contribute to immunity against influenza by limiting viral replication. It is therefore surprising that rigorous statistical tests have failed to find evidence of positive selection in the epitopes targeted by CD8(+) T cells. Here we use a n...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01571-15
更新日期:2015-11-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infects human cells by binding to surface CD4 molecules and directly fusing with the cell membrane. Although mouse cells expressing human CD4 bind HIV, they do not become infected, apparently because of a block in membrane fusion. To study this problem, we constructed a recombinant v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2149-2156.1990
更新日期:1990-05-01 00:00:00
abstract::Antisera produced in mice recognize primarily type-specific antigenic determinants on both the major core protein, p30, and the major envelope proteins, gp70 and p15(E), of the endogenous leukemia viruses (MuLV) of BALB/c mice. Three different mouse sera were investigated in detail. (i) Antisera prepared in C57BL/6 mi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.345-349.1979
更新日期:1979-10-01 00:00:00
abstract::Type III interferons (IFNs) (interleukin-28/29 or lambda interferon [IFN-lambda]) are cytokines with IFN-like activities. Here we show that several classes of viruses induce expression of IFN-lambda1 and -lambda2/3 in similar patterns. The IFN-lambdas were-unlike alpha/beta interferon (IFN-alpha/beta)-induced directly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.9.4501-4509.2006
更新日期:2006-05-01 00:00:00
abstract::Human norovirus (HuNoV) is a leading cause of acute gastroenteritis in both developed and developing countries. Studies of HuNoV host cell interactions are limited by the lack of a simple, robust cell culture system. Due to their diverse HuNoV-like biological features, including histo-blood group antigen (HBGA) bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00869-19
更新日期:2019-10-29 00:00:00
abstract::MVL2, an enveloped double-stranded DNA mycoplasmavirus, causes a nonlytic infection of Acholeplasma laidlawii leading to the establishment of a persistent infection. Persistently infected clones were found to be resistant to superinfection by homologous virus, but could be infected by heterologous virus. Cells in a pe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.254-261.1978
更新日期:1978-10-01 00:00:00
abstract::The human cytomegalovirus (HCMV) major immediate-early (IE) proteins share an 85-amino-acid N-terminal domain specified by exons 2 and 3 of the major IE region, UL122-123. We have constructed IE Delta30-77, a recombinant virus that lacks the majority of IE exon 3 and consequently expresses smaller forms of both IE1 72...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7438-7452.2005
更新日期:2005-06-01 00:00:00
abstract::Antiserum against murine cytomegalovirus produced in the rabbit contained complement (C')-requiring neutralizing (CRN) antibody. The proportion of CRN was extremely high (up to 98%) during the early portion of an immunization procedure, whereas the antisera produced late had a much lower proportion that required C'. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1620-1621.1973
更新日期:1973-12-01 00:00:00
abstract::Substitution of a methionine residue at position 79 in poliovirus protein 3A with valine or threonine caused defective viral RNA synthesis, manifested as delayed onset and reduced yield of viral RNA, in HeLa cells transfected with a luciferase-containing replicon. Viruses containing these same mutations produced small...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12679-12691.2003
更新日期:2003-12-01 00:00:00