Abstract:
:The vpr gene product of human immunodeficiency virus type (HIV-1) is a virion-associated regulatory protein. A transferable virion association motif for Vpr is located in the p6 domain of the HIV-1 Gag polyprotein. To map the sequences in p6 that are involved in Vpr incorporation, we analyzed the ability of mutant forms of p6 to direct the incorporation of Vpr into chimeric viral particles. Our results show that the determinants which govern Vpr incorporation are largely confined to a C-terminal region of the p6 domain. Within this region, three hydrophobic residues in a highly conserved sequence motif (L-X-S-L-F-G) are absolutely required. Remarkably, the transfer of the conserved motif and of a single flanking residue to a heterologous Gag polyprotein was sufficient to transfer the ability to incorporate Vpr at moderate levels. The transfer of residues 32 to 46 of p6 led to Vpr incorporation levels that were comparable to those obtained with full-length HIV-1 Gag protein, indicating that this region contains essentially all the information required for efficient Vpr incorporation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kondo E,Göttlinger HGdoi
10.1128/JVI.70.1.159-164.1996subject
Has Abstractpub_date
1996-01-01 00:00:00pages
159-64issue
1eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::Autoradiographic analyses of deoxyribonucleic acid (DNA) synthesis in randomly growing KB cell cultures infected with equine abortion virus (EAV) suggested that viral DNA synthesis was initiated only at times that coincided with the entry of noninfected control cells into the S phase of the cell cycle. Synchronized cu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.736-748.1971
更新日期:1971-06-01 00:00:00
abstract::We previously reported that a mutant Rous sarcoma virus (RSV) with an alternate polypurine tract (PPT), DuckHepBFlipPPT, had unexpectedly high titers and that the PPT was miscleaved primarily at one position following a GA dinucleotide by the RNase H of reverse transcriptase (RT). This miscleavage resulted in a portio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00499-08
更新日期:2008-09-01 00:00:00
abstract:UNLABELLED:Increasing data suggest that NK cells can mediate antiviral activity in HIV-1-infected humans, and as such, novel approaches harnessing the anti-HIV-1 function of both T cells and NK cells represent attractive options to improve future HIV-1 immunotherapies. Chronic progressive HIV-1 infection has been assoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00924-14
更新日期:2014-08-01 00:00:00
abstract::The structural gene for diphtherial toxin is present in corynebacteriophage beta. Previous studies located several point mutations within the tox locus and determined the orientation of transcription of the toxin structural gene. The production of maximal quantities of toxin by Corynebacterium diphtheriae C7(beta) occ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.946-954.1981
更新日期:1981-03-01 00:00:00
abstract::Borna disease virus (BDV) is an enveloped virus with a genome organization characteristic of Mononegavirales. However, based on its unique features, BDV is considered the prototypic member of a new virus family, Bornaviridae, within the order Mononegavirales. We have described the establishment of a reverse genetics s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6544-6550.2005
更新日期:2005-05-01 00:00:00
abstract::Phenol-extracted, infectious deoxyribonucleic acid (DNA) species from phi105 phage particles, from phi105 lysogenic bacteria, and from induced phi105 lysogenic bacteria were sedimented in sucrose gradients. Infectious DNA from phi105 particles sedimented like the bulk of mature phage DNA in neutral sucrose. Infectivit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.5.604-608.1970
更新日期:1970-05-01 00:00:00
abstract::By using temperature-sensitive (ts) and suppressor-sensitive (sus) mutants, 11 essential genes have been identified in phage phi105. The order of the genes has been established in two- and three-factor crosses. The genes can be arranged in a linear order; this order is identical in the vegetative phage and in the prop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.6.760-767.1970
更新日期:1970-12-01 00:00:00
abstract::BALB/c JLS V9 cells recently infected with Harvey sarcoma virus-murine leukemia virus (HSV-MuLV) complex contained unintegrated HSV linear DNA of 6.0-kilobase pair mass. The cells also contained two HSV closed circular DNA species along with MuLV-encoded linear and closed circular DNA species. HSV 6.0-kilobase pair li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.30-39.1979
更新日期:1979-10-01 00:00:00
abstract::Relatively little is known at the functional genomic level about the global host response to human immunodeficiency virus type 1 (HIV-1) infection. Microarray analyses by several laboratories, including our own, have revealed that HIV-1 infection causes significant changes in host mRNA abundance and regulation of seve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00288-07
更新日期:2007-07-01 00:00:00
abstract::We used a transfected L cell and a vaccinia vector carrying the herpes simplex virus type 1 (HSV-1) gene coding for glycoprotein D (gD) to characterize HSV-specific T-cell responses. Various studies with mice revealed that the vectors could stimulate some HSV-specific T-cell responses. Although the majority of the T c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.3.726-734.1987
更新日期:1987-03-01 00:00:00
abstract::Mixed infection of a cell by vesicular stomatitis virus (VSV) and retroviruses results in the production of progeny virions bearing the genome of one virus encapsidated by the envelope proteins of the other. The mechanism for the phenomenon of pseudotype formation is not clear, although specific recognition of a viral...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1202-1207.1991
更新日期:1991-03-01 00:00:00
abstract::The major immediate-early (MIE) transactivator proteins of cytomegaloviruses (CMV) play a pivotal role in the initiation of virus-host cell interactions. Therefore, cis- and trans-acting factors influencing the expression of these proteins through their upstream promoter-enhancer regions are important determinants of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.516-529.1993
更新日期:1993-01-01 00:00:00
abstract::In order to clarify the effect of an accumulation of amino acid substitutions on the hemadsorption character of the influenza AH3 virus hemagglutinin (HA) protein, we introduced single-point amino acid changes into the HA1 domain of the HA proteins of influenza viruses isolated in 1968 (A/Aichi/2/68) and 1997 (A/Sydne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6472-6477.2005
更新日期:2005-05-01 00:00:00
abstract::The cytoplasmic assembly of vaccinia virus is reversibly blocked by the antibiotic rifampin, leading to the accumulation of partially membrane-delineated rifampin bodies in infected cells. Rifampin-resistant vaccinia virus mutants have point mutations in the D13L gene, which is controlled by a late promoter and expres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1103-1114.1994
更新日期:1994-02-01 00:00:00
abstract::The molecular basis of the temperature-sensitive (ts) phenotype of P3/Sabin, the type 3 vaccine strain of poliovirus, was investigated in light of the known correlation between ts and attenuation phenotypes. A phenylalanine at residue 91 of the capsid protein VP3 was a major determinant of both phenotypes, and attenua...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5225-5231.1991
更新日期:1991-10-01 00:00:00
abstract::The terminal 500-base-pair alpha sequence of the herpes simplex virus 1 genome contains signals for cleavage (Pac1 and Pac2) of unit-length DNA molecules from concatemers in unique stretches of sequences designated Ub and Uc, respectively, and a cis site for cleavage designated DR1. We report that nuclear extracts fro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1059-1068.1989
更新日期:1989-03-01 00:00:00
abstract::The Vpu protein is a human immunodeficiency virus type 1 (HIV-1)-specific accessory protein that is required for the efficient release of viral particles from infected cells. Even though HIV-2 does not encode Vpu, we found that this virus is nevertheless capable of efficiently releasing virus particles. In fact, the r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.820-829.1996
更新日期:1996-02-01 00:00:00
abstract::Leishmania RNA virus (LRV) is a double-stranded RNA virus that infects some strains of the protozoan parasite leishmania As with other totiviruses, LRV presumably expresses its polymerase by a ribosomal frameshift, resulting in a capsid-polymerase fusion protein. We have demonstrated previously that an LRV capsid-poly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10448-10455.2003
更新日期:2003-10-01 00:00:00
abstract::Herpes simplex virus type 1 glycoprotein H (HSV-1 gH) was synthesized in an inducible mammalian cell expression system, and its properties were examined. The gH coding sequence, together with the stable 5' untranslated leader sequence from xenopus beta-globin, was placed under control of the strong promoter from the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4744-4755.1989
更新日期:1989-11-01 00:00:00
abstract::Viral particles infecting some stocks of the protozoan parasite Leishmania braziliensis subsp. guyanensis contain a double-stranded RNA genome of ca. 5 kbp and are associated with an RNA-dependent RNA polymerase which synthesizes in vitro double-stranded and single-stranded, genome-length transcripts. The majority of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4211-4215.1991
更新日期:1991-08-01 00:00:00
abstract:UNLABELLED:Broadly neutralizing antibodies (bNAbs) specific for conserved epitopes on the HIV-1 envelope (Env) are believed to be essential for protection against multiple HIV-1 clades. However, vaccines capable of stimulating the production of bNAbs remain a major challenge. Given that polyreactivity and autoreactivit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02904-14
更新日期:2015-04-01 00:00:00
abstract::Thanks to new technologies which enable rapid and unbiased screening for viral nucleic acids in clinical specimens, an impressive number of previously unknown viruses have recently been discovered. Two research groups independently identified a novel negative-strand RNA virus, now designated avian bornavirus (ABV), in...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.02567-09
更新日期:2010-07-01 00:00:00
abstract::Wild-type herpes simplex virus 1 (HSV-1) multiplies, spreads, and rapidly destroys cells of the murine central nervous system (CNS). In contrast, mutants lacking both copies of the gamma(1)34.5- gene have been shown to be virtually lacking in virulence even after direct inoculation of high-titered virus into the CNS o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5560-5569.1997
更新日期:1997-07-01 00:00:00
abstract::In some type C retroviruses, translation of the pol gene appears to require translational suppression of the proximal gag amber codon. To identify the region of the viral nucleic acid responsible for synthesis of the pol gene products, a 300-base-pair DNA fragment containing the stop codon from a type C murine virus (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3574-3580.1988
更新日期:1988-10-01 00:00:00
abstract::Among the numerous immunological abnormalities observed in chronically human immunodeficiency virus (HIV)-infected individuals, perturbations in memory CD4 T cells are thought to contribute specifically to disease pathogenesis. Among these, functional imbalances in the frequencies of T regulatory cells (Tregs) and int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02163-18
更新日期:2019-04-17 00:00:00
abstract::Reoviruses are encapsidated double-stranded RNA viruses that cause systemic disease in mice after peroral (p.o.) inoculation and primary replication in the intestine. In this study, we define components of the immune system involved in the clearing of reovirus from the proximal small intestine. The intestines of immun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.1109-1116.1996
更新日期:1996-02-01 00:00:00
abstract::Mouse mammary tumor viruses (MMTVs) contain distinct membrane glycoproteins of 52,000 daltons (gp52) and 36,000 daltons (gp36). We report here the development of new radioimmunoassays for gp36, using gp36 purified by hydrophobic chromatography and gel filtration. These assays demonstrate that gp36 has both type-speci...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.2.334-340.1979
更新日期:1979-08-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) uses a variety of chemokine receptors as coreceptors for virus entry, and the ability of the virus to be neutralized by antibody may depend on which coreceptors are used. In particular, laboratory-adapted variants of the virus that use CXCR4 as a coreceptor are highly sensit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1886-1893.1998
更新日期:1998-03-01 00:00:00
abstract::We found that recircularized high-risk (type 16 and 18) and low-risk mucosal (type 6b and 11) and cutaneous (type 5 and 8) human papillomavirus (HPV) genomes replicate readily when delivered into U2OS cells by electroporation. The replication efficiency is dependent on the amount of input HPV DNA and can be followed f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01985-10
更新日期:2011-04-01 00:00:00
abstract::Polydnaviridae is a unique family of DNA viruses that are symbiotically associated with parasitoid wasps. Upon oviposition, wasps inject these viruses into their hosts, where they cause several physiological alterations, including suppression of the cellular immune response. Here we report that expression of the glc1....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1861-1870.2005
更新日期:2005-02-01 00:00:00