Abstract:
:The human cytomegalovirus (HCMV) major immediate-early (IE) proteins share an 85-amino-acid N-terminal domain specified by exons 2 and 3 of the major IE region, UL122-123. We have constructed IE Delta30-77, a recombinant virus that lacks the majority of IE exon 3 and consequently expresses smaller forms of both IE1 72- and IE2 86-kDa proteins. The mutant virus is viable but growth impaired at both high and low multiplicities of infection and exhibits a kinetic defect that is not rescued by growth in fibroblasts expressing IE1 72-kDa protein. The kinetics of mutant IE2 protein accumulation in IE Delta30-77 virus-infected cells are approximately normal compared to wild-type virus-infected cells, but the IE Delta30-77 virus is delayed in expression of early viral genes, including UL112-113 and UL44, and does not sustain expression of mutant IE1 protein as the infection progresses. Additionally, cells infected with IE Delta30-77 exhibit altered expression of cellular proteins compared to wild-type HCMV-infected cells. PML is not dispersed but is retained at ND10 sites following infection with IE Delta30-77 mutant virus. While the deletion mutant retains the ability to mediate the stabilization of cyclin B1, cdc6, and geminin in infected cells, its capacity to upregulate the expression of cyclin E has been reduced. These data indicate that the activity of one or both of the HCMV major IE proteins is required in vivo for the modulation of cell cycle proteins observed in cells infected with wild-type HCMV.
journal_name
J Viroljournal_title
Journal of virologyauthors
White EA,Spector DHdoi
10.1128/JVI.79.12.7438-7452.2005subject
Has Abstractpub_date
2005-06-01 00:00:00pages
7438-52issue
12eissn
0022-538Xissn
1098-5514pii
79/12/7438journal_volume
79pub_type
杂志文章abstract::The E3 complex transcription unit of adenovirus encodes four major mRNAs (a, c, f, and h) and two minor (d and e) mRNAs with overlapping exons, alternative splice sites, and two polyadenylation sites, termed E3A (upstream) and E3B (downstream). mRNAs a and d use the E3A polyadenylation site, and mRNAs c, e, f, and h u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5914-5923.1992
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3451-3454.1998
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pub_type: 杂志文章
doi:10.1128/JVI.70.7.4205-4209.1996
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1984-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3620-3630.1994
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1970-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05988-11
更新日期:2012-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03268-12
更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.23.3.517-523.1977
更新日期:1977-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02497-09
更新日期:2010-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.77.11.6533-6540.2003
更新日期:2003-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1987-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.74.22.10458-10467.2000
更新日期:2000-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01101-19
更新日期:2020-04-16 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00601-08
更新日期:2008-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.881-885.1990
更新日期:1990-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.12012-12021.2004
更新日期:2004-11-01 00:00:00
abstract:UNLABELLED:The four serotypes of dengue virus (DENV) cause the most important and rapidly emerging arboviral diseases in humans. The recent phase 2b and 3 studies of a tetravalent dengue vaccine reported a moderate efficacy despite the presence of neutralizing antibodies, highlighting the need for a better understandin...
journal_title:Journal of virology
pub_type: 杂志文章,随机对照试验
doi:10.1128/JVI.00273-15
更新日期:2015-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.664-671.1972
更新日期:1972-04-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) is a large (150-kb) double-stranded DNA virus that forms latent infections in neuronal cells of the human peripheral nervous system. Previous work determined that the HSV-1 genome is found in an ordered nucleosomal structure during latent infection. However, during lytic infection, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.10178-10186.2004
更新日期:2004-09-01 00:00:00
abstract::Bovine leukemia virus (BLV) is silent in most cells detectable in vivo, and the repression of its expression allows BLV to evade the host's immune response. In this study, we examined whether CpG methylation of DNA might be involved in the regulation of the expression of BLV in vivo. To investigate the effects of CpG ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4423-4430.2003
更新日期:2003-04-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) is a recently described arterivirus responsible for disease in swine worldwide. Comparative sequence analysis of 3'-terminal structural genes of the single-stranded RNA viral genome revealed the presence of two genotypic classes of PRRSV, represented by the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.270-280.1999
更新日期:1999-01-01 00:00:00