Abstract:
:Human oncolytic adenoviruses have been used in clinical trials targeting cancers of epithelial origin. To gain a better understanding of the infectious cycle of adenovirus in normal human squamous tissues, we examined the viral infection process in organotypic cultures of primary human keratinocytes. We show that for the infection to occur, wounding of the epithelium is required. In addition, infection appears to initiate at the basal or parabasal cells that express the high-affinity coxsackievirus-adenovirus receptor, CAR, whereas the productive phase takes place in differentiated cells. This is due, at least in part, to the differentiation-dependent activation of the E1A and E2A early promoters and E4 promoters. We also show that adenovirus infection triggers a response mediated by the abnormal accumulation of cyclin E and p21cip1 proteins similar to the one previously observed in human papillomavirus-infected tissues. However, the virus seems to be able to overcome it, at least partially.
journal_name
J Viroljournal_title
Journal of virologyauthors
Noya F,Balagué C,Banerjee NS,Curiel DT,Broker TR,Chow LTdoi
10.1128/jvi.77.11.6533-6540.2003subject
Has Abstractpub_date
2003-06-01 00:00:00pages
6533-40issue
11eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::Enterovirus 71 (EV71) (genus Enterovirus, family Picornaviridae), a common cause of hand, foot, and mouth disease (HFMD), may also cause severe neurological diseases, such as encephalitis and poliomyelitis-like paralysis. To examine the genetic diversity and rate of evolution of EV71, we have determined and analyzed c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.9969-9975.1999
更新日期:1999-12-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) vpr inhibits the replication of tumor cell lines and peripheral blood mononuclear cells. Here it is demonstrated that expression of vpr, either in the context of a provirus or from an independent genetic element, induces a discrete cell cycle arrest, with cells containing 4N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6859-6864.1995
更新日期:1995-11-01 00:00:00
abstract::Interleukin-1 receptor antagonist (IL-1ra) gene polymorphisms in 83 human immunodeficiency virus (HIV)-seropositive women were evaluated. Fourteen of the subjects (16.9%) were homozygous for IL-1ra allele 2 (IL-1RN*2). These women had a lower median level of HIV RNA than did women homozygous for allele 1 (IL-1RN*1) (P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.6242-6244.2001
更新日期:2001-07-01 00:00:00
abstract::An unusual human retrovirus was isolated from two patients with persistent generalized lymphadenopathy who originate from West-Central Africa and are currently residing in Belgium. Although the virus shared a number of the same biological and morphological properties as human immunodeficiency retrovirus type 1 (HIV-1)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1207-1216.1990
更新日期:1990-03-01 00:00:00
abstract::Following respiratory syncytial virus (RSV) challenge, mice immunized with RSV G or with formalin-inactivated RSV (FI-RSV) exhibit severe disease associated with type 2 cytokine production and pulmonary eosinophilia. This has led to the proposal that the presence of RSV G is the factor in FI-RSV that induces disease-e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.11.6024-6032.2004
更新日期:2004-06-01 00:00:00
abstract::Noroviruses are the causative agents of the majority of viral gastroenteritis outbreaks in humans. During the past 15 years, noroviruses of genotype GGII.4 have caused four epidemic seasons of viral gastroenteritis, during which four novel variants (termed epidemic variants) emerged and displaced the resident viruses....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00674-07
更新日期:2007-09-01 00:00:00
abstract::Complement, part of the innate immune system, acts to remove pathogens and unwanted host material. Complement is known to function in all tissues, including the central nervous system (CNS). In this study, we demonstrated the importance of the complement system within the CNS in the development of behavioral seizures ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00422-10
更新日期:2010-07-01 00:00:00
abstract::Complementary DNA clones encoding the murine homolog (mCAR) of the human coxsackievirus and adenovirus receptor (CAR) were isolated. Nonpermissive CHO cells transfected with mCAR cDNA became susceptible to infection by coxsackieviruses B3 and B4 and showed increased susceptibility to adenovirus-mediated gene transfer....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.415-419.1998
更新日期:1998-01-01 00:00:00
abstract::It has been proposed recently that the cell surface peptidase CD26 acts in concert with CD4, the human immunodeficiency virus (HIV) primary receptor molecule, to mediate HIV entry into permissive cells. We have failed to detect significant levels of CD26 cell surface expression and enzymatic activity in a number of co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6535-6546.1994
更新日期:1994-10-01 00:00:00
abstract::We have previously shown that delivery of the porcine type I interferon gene (poIFN-α/β) with a replication-defective human adenovirus vector (adenovirus 5 [Ad5]) can sterilely protect swine challenged with foot-and-mouth disease virus (FMDV) 1 day later. However, the need of relatively high doses of Ad5 limits the ap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03462-12
更新日期:2013-05-01 00:00:00
abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) nsp1 protein has unique biological functions that have not been described in the viral proteins of any RNA viruses; expressed SARS-CoV nsp1 protein has been found to suppress host gene expression by promoting host mRNA degradation and inhibiting translation....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02472-07
更新日期:2008-05-01 00:00:00
abstract::Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 mRNA were isolated and sequenced. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.464-467.1991
更新日期:1991-01-01 00:00:00
abstract::The human cytomegalovirus (HCMV) major immediate-early (MIE) enhancer contains five functional cyclic AMP (cAMP) response elements (CRE). Because the CRE in their native context do not contribute appreciably to MIE enhancer/promoter activity in lytically infected human fibroblasts and NTera2 (NT2)-derived neurons, we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01524-06
更新日期:2007-06-01 00:00:00
abstract::Hepatitis B virus (HBV) is an enveloped DNA virus that presumably buds at intracellular membranes of infected cells. HBV budding involves two endocytic host proteins, the ubiquitin-interacting adaptor gamma 2-adaptin and the Nedd4 ubiquitin ligase. Here, we demonstrate that HBV release also requires the cellular machi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00479-07
更新日期:2007-09-01 00:00:00
abstract::Chikungunya virus (CHIKV) is a mosquito-borne alphavirus responsible for chikungunya fever. Nonstructural protein 2 (nsP2), a multifunctional protein essential for viral replication, has an N-terminal helicase region (nsP2h), which as both nucleotide triphosphatase and RNA triphosphatase activities, as well as a C-ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01470-20
更新日期:2020-12-16 00:00:00
abstract::Wild-type human respiratory syncytial virus (HRSV) is a poor inducer of alpha/beta interferons (IFN-alpha/beta). However, recombinant HRSV lacking the NS1 and NS2 genes (Delta NS1/2) induced high levels of IFN-alpha and -beta in human pulmonary epithelial cells (A549) as well as in macrophages derived from primary hum...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.8.4363-4369.2004
更新日期:2004-04-01 00:00:00
abstract::The effect of guanidine on the replication of the group A arboviruses, Sindbis virus, and Semliki Forest virus (SFV) was studied. Guanidine rapidly, but reversibly, inhibited SFV ribonucleic acid (RNA) synthesis. The synthesis of all species of viral RNA was inhibited, but that of ribonuclease-resistant forms was leas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.5.628-636.1970
更新日期:1970-11-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and high mortality rates in newborn piglets, leading to massive losses to the swine industry worldwide during recent epidemics. Intense research efforts are now focusing on defining viral characteristics that confer a growth advantage, pathogenicity, or cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01660-16
更新日期:2017-01-03 00:00:00
abstract::Apoptosis of neurons and astrocytes is induced by human immunodeficiency type 1 (HIV-1) infection in vitro and has been demonstrated in brain tissue from patients with AIDS. We analyzed a panel of diverse HIV-1 primary isolates for the ability to replicate and induce neuronal and astrocyte apoptosis in primary human b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.897-906.1999
更新日期:1999-02-01 00:00:00
abstract::Recent in vitro proteomics screens revealed that many host proteins could interact with the replication proteins of Tomato bushy stunt virus (TBSV), which is a small, plus-stranded RNA virus (Z. Li, D. Barajas, T. Panavas, D. A. Herbst, and P. D. Nagy, J. Virol. 82:6911-6926, 2008). To further our understanding of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00789-09
更新日期:2009-11-01 00:00:00
abstract::The capsid mRNA transcripts of human bocavirus 1 (HBoV1) can be generated by alternative splicing from the mRNA precursor transcribed from the P5 promoter. However, the alternative translation regulation mechanism of capsid mRNA transcripts is largely unknown. Here we report that the polycistronic capsid mRNA transcri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00443-18
更新日期:2018-10-12 00:00:00
abstract::We investigated the primary cellular immune responses to human immunodeficiency virus type 1 (HIV-1) Env and Gag proteins elicited by recombinant vesicular stomatitis viruses (rVSVs). The primary response to Env peaked 5 to 7 days after intraperitoneal vaccination, at which time 40% of CD8(+) cells were Env tetramer p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.6.2730-2738.2002
更新日期:2002-03-01 00:00:00
abstract::The membrane-proximal region of the human immunodeficiency virus type 1 (HIV-1) transmembrane protein (TM) is critical for envelope (Env)-mediated membrane fusion and contains the target for broadly reactive neutralizing antibody 2F5. It has been proposed that 2F5 neutralization might involve interaction of its long, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2539-2547.2006
更新日期:2006-03-01 00:00:00
abstract::The initiation of human immunodeficiency virus type 1 (HIV-1) reverse transcription occurs at a site in the viral RNA genome which is designated the primer-binding site (PBS). The HIV-1 PBS is an 18-nucleotide sequence that is complementary to the 3'-terminal 18 nucleotides of tRNA(3Lys), which is used as the primer f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6021-6029.1995
更新日期:1995-10-01 00:00:00
abstract::Purified Friend viruses obtained from chronically infected tissue cultures were studied under the electron microscope in an effort to correlate the fine structure of the particles to their leukemogenic activity under varied experimental conditions, i.e., temperature treatments and exposure to Tween 80, amyl acetate, o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.4.365-375.1968
更新日期:1968-04-01 00:00:00
abstract::Superinfection exclusion is the ability of an established virus infection to interfere with infection by a second virus. In this study, we found that Huh-7.5 cells acutely infected with hepatitis C virus (HCV) genotype 2a (chimeric strain J6/JFH) and cells harboring HCV genotype 1a, 1b, or 2a full-length or subgenomic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01748-06
更新日期:2007-04-01 00:00:00
abstract::Cells of the established MvlLu mink line spontaneously released a reverse transcriptase-containing virus after long-term passage in tissue culture. By molecular hybridization, DNA of normal mink cells was found to possess extensive nucleotide sequence homology with a reverse-transcription product of the viral genome, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.129-137.1978
更新日期:1978-01-01 00:00:00
abstract::Interleukin-15 (IL-15), natural killer (NK) cells, and NK T (NKT) cells, components of the innate immune system, are known to contribute to defense against pathogens, including viruses. Here we report that IL-15(-/-) (NK(-) and NKT(-/+)) mice and RAG-2(-/-)/gamma(c)(-/-) (NK(-) and NKT(-)) mice that lack all lymphoid ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10168-10171.2003
更新日期:2003-09-01 00:00:00
abstract::We have studied initiation and regulation of early transcription of simian virus (SV40) DNA in vitro by eucaryotic RNA polymerase II, using both a crude HeLa cell extract and a partially purified calf thymus polymerase supplemented with a HeLa cell S100 fraction. Analysis of initiation sites by primer-directed cDNA sy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.449-461.1982
更新日期:1982-02-01 00:00:00
abstract::We have identified a trans-dominant mutant form of the herpes simplex virus (HSV) DNA-binding protein ICP8 which inhibits viral replication. When expressed by the V2.6 cell line, the mutant gene product inhibited wild-type HSV production by 50- to 150-fold when the multiplicity of infection was less than 5. Production...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2666-2675.1991
更新日期:1991-05-01 00:00:00