Abstract:
:Hepatitis C virus (HCV) RNA genome replicates within the ribonucleoprotein (RNP) complex in the modified membranous structures extended from endoplasmic reticulum. A proteomic analysis of HCV RNP complexes revealed the association of oxysterol binding protein (OSBP) as one of the components of these complexes. OSBP interacted with the N-terminal domain I of the HCV NS5A protein and colocalized to the Golgi compartment with NS5A. An OSBP-specific short hairpin RNA that partially downregulated OSBP expression resulted in a decrease of the HCV particle release in culture supernatant with little effect on viral RNA replication. The pleckstrin homology (PH) domain located in the N-terminal region of OSBP targeted this protein to the Golgi apparatus. OSBP deletion mutation in the PH (DeltaPH) domain failed to localize to the Golgi apparatus and inhibited the HCV particle release. These studies suggest a possible functional role of OSBP in the HCV maturation process.
journal_name
J Viroljournal_title
Journal of virologyauthors
Amako Y,Sarkeshik A,Hotta H,Yates J 3rd,Siddiqui Adoi
10.1128/JVI.00958-09subject
Has Abstractpub_date
2009-09-01 00:00:00pages
9237-46issue
18eissn
0022-538Xissn
1098-5514pii
JVI.00958-09journal_volume
83pub_type
杂志文章abstract::DNA sequences encoding the surface envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) were amplified by PCR from uncultured peripheral blood mononuclear cells obtained from patients with serologically defined HIV-1/HIV-2 mixed infections from Bombay, India. HIV-1-specific PCR prod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2161-2168.1994
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journal_title:Journal of virology
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doi:10.1128/JVI.70.1.663-666.1996
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1712-1716.1987
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.866-876.1980
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pub_type: 杂志文章
doi:10.1128/JVI.00053-06
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.962-965.1990
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.479-487.1988
更新日期:1988-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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abstract:UNLABELLED:CD4+ T cells play a pivotal role in the control of chronic viral infections. Recently, nontraditional CD4+ T cell functions beyond helper effects have been described, and a role for cytolytic CD4+ T cells in the control of HIV infection has been suggested. We define here the transcriptional, phenotypic, and ...
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.6.2936-2951.2002
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pub_type: 杂志文章
doi:10.1128/JVI.64.11.5412-5419.1990
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03166-13
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.2.275-287.1978
更新日期:1978-08-01 00:00:00
abstract::Herpes simplex virus type 1 glycoprotein H (HSV-1 gH) was synthesized in an inducible mammalian cell expression system, and its properties were examined. The gH coding sequence, together with the stable 5' untranslated leader sequence from xenopus beta-globin, was placed under control of the strong promoter from the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4744-4755.1989
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2019-01-04 00:00:00
abstract::Wild-type herpes simplex virus 1 (HSV-1) multiplies, spreads, and rapidly destroys cells of the murine central nervous system (CNS). In contrast, mutants lacking both copies of the gamma(1)34.5- gene have been shown to be virtually lacking in virulence even after direct inoculation of high-titered virus into the CNS o...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1258-1263.1994
更新日期:1994-02-01 00:00:00