Strand-specific RNA synthesis defects in a poliovirus with a mutation in protein 3A.

abstract：:The vaccinia virus F10 protein is one of two virally encoded protein kinases. A phenotypic analysis of infections involving a tetracycline-inducible recombinant (vDeltaiF10) indicated that F10 is involved in the early stages of virion morphogenesis, as previously reported for the mutants ts28 and ts15. The proteins en...

journal_title：Journal of virology

authors： Punjabi A,Traktman P

更新日期：2005-02-01 00:00:00

abstract：:Harvard Medical School convened a meeting of biomedical and clinical experts on 5 March 2015 on the topic of "Rethinking the Response to Emerging Microbes: Vaccines and Therapeutics in the Ebola Era," with the goals of discussing the lessons from the recent Ebola outbreak and using those lessons as a case study to aid...

journal_title：Journal of virology

authors： Knipe DM,Whelan SP

更新日期：2015-08-01 00:00:00

abstract：:Normal replication of Moloney murine leukemia virus (MoMLV) requires the integration of a DNA copy of the viral RNA genome into a chromosome of the host. In this work, we characterize the DNA sequences at the ends of the linear proviral precursor that are required for integration in the presence of MoMLV integration p...

journal_title：Journal of virology

authors： Bushman FD,Craigie R

更新日期：1990-11-01 00:00:00

abstract：:We cloned and analyzed the integrated human papillomavirus type 16 (HPV-16) genomes that are present in the human cervical carcinoma cell lines SiHa and CaSki. The single HPV-16 genome in the SiHa line was cloned as a 10-kilobase (kb) HindIII fragment. Integration of the HPV-16 genome occurred at bases 3132 and 3384 w...

journal_title：Journal of virology

authors： Baker CC,Phelps WC,Lindgren V,Braun MJ,Gonda MA,Howley PM

更新日期：1987-04-01 00:00:00

abstract：:Immune responses and the components of protective immunity following norovirus infection in humans are poorly understood. Although antibody responses following norovirus infection have been partially characterized, T cell responses in humans remain largely undefined. In contrast, T cells have been shown to be essentia...

journal_title：Journal of virology

authors： LoBue AD,Lindesmith LC,Baric RS

更新日期：2010-09-01 00:00:00

abstract：:We have previously characterized five proteins induced by the presence of the E2 open reading frame (ORF) region of bovine papillomavirus type 1 (BPV-1) in C127 mouse fibroblasts (R. M. Levenson, U. G. Brinckmann, M. K. O'Banion, E. J. Androphy, J. T. Schiller, F. Tabatabai, L. P. Turek, K. Neary, M. T. Chin, T. R. Br...

journal_title：Journal of virology

authors： O'Banion MK,Young DA

更新日期：1991-07-01 00:00:00

abstract：:A soluble system was developed that could support DNA replication in simian virus 40 (SV40) chromosomes. DNA synthesis in this system required the presence of purified SV40 large tumor antigen, SV40 chromosomes prepared from virus-infected monkey cells, a crude extract from HeLa cells, and several low-molecular-weight...

journal_title：Journal of virology

authors： Sugasawa K,Murakami Y,Miyamoto N,Hanaoka F,Ui M

更新日期：1990-10-01 00:00:00

abstract：:To identify retroviral antigenic determinants recognized by CD4+ T helper cells during tumor rejection, we established four noncytolytic, helper-type, CD4+ T-cell clones by limiting dilution cultures of mixed lymphocyte-tumor cultures from mice immune to a Friend virus-induced tumor, FBL-3. Among these, three T helper...

journal_title：Journal of virology

authors： Iwashiro M,Kondo T,Shimizu T,Yamagishi H,Takahashi K,Matsubayashi Y,Masuda T,Otaka A,Fujii N,Ishimoto A

更新日期：1993-08-01 00:00:00

abstract：:Cloned avian leukosis viral DNAs were mutagenized in the long terminal repeat, in the leader sequence for env mRNA, and at the poly-env junction. The effect of these mutations in the viral DNA upon its ability to direct virus production or env mRNA synthesis was analyzed by microinjecting the mutant DNAs into chicken ...

journal_title：Journal of virology

authors： Pugatsch T,Stacey DW

更新日期：1982-08-01 00:00:00

abstract：:Natural Killer (NK) cells are important in the immune response to a number of viruses; however, the mechanisms used by NK cells to discriminate between healthy and virus-infected cells are only beginning to be understood. Infection with vaccinia virus provokes a marked increase in the susceptibility of target cells to...

journal_title：Journal of virology

authors： Chisholm SE,Reyburn HT

更新日期：2006-03-01 00:00:00

abstract：:The induction of an interferon-induced antiviral state is a powerful cellular response against viral infection that limits viral spread. Here, we show that a preexisting antiviral state inhibits the replication of influenza A viruses in human A549 cells by preventing transport of the viral genome to the nucleus and th...

journal_title：Journal of virology

authors： Xiao H,Killip MJ,Staeheli P,Randall RE,Jackson D

更新日期：2013-12-01 00:00:00

abstract：:We compared the intracellular location of the product of the c-fps proto-oncogene, NCP98, with that of its viral homolog P140, the transforming protein of Fujinami sarcoma virus. Using the technique of biochemical subcellular fractionation, we determined that 60 to 90% of NCP98 and its associated kinase activity are i...

journal_title：Journal of virology

authors： Young JC,Martin GS

更新日期：1984-12-01 00:00:00

abstract：:At mucosal sites, papillomavirus genomes can persist in the epithelial basal layer following immune-mediated regression. Subsequent T-cell depletion stimulates a 3- to 5-log increase in the viral copy number, to levels associated with productive infection. Reappearance of microlesions was rare within the short time fr...

journal_title：Journal of virology

authors： Maglennon GA,McIntosh PB,Doorbar J

更新日期：2014-01-01 00:00:00

abstract：:Several aspects of the terminal stages of T4 head maturation were investigated using ts and am mutants blocked at single steps of the assembly pathway. We had previously found that cells infected with mutants of gene 13, e.g., tsN38 and amE609, accumulated both stable (10 to 20%)- and fragile (80%)-filled head precurs...

journal_title：Journal of virology

authors： Hamilton DL,Luftig RB

更新日期：1976-02-01 00:00:00

abstract：:Simian immunodeficiency virus (SIV) has been shown to progress through a number of changes that lead to the emergence of pathogenic viral variants in macaques initially infected with a mildly cytopathic variant, SIVMneCL8. One of these late-stage isolates, SIVMne170, replicates to high levels in vivo and causes a rapi...

journal_title：Journal of virology

authors： Voronin Y,Overbaugh J,Emerman M

更新日期：2005-12-01 00:00:00

abstract：:We determined the sequence of the gene for the virion transactivator protein Vmw65 of herpes simplex virus type 2 (HSV-2), strain 333. An analysis of the coding sequence revealed an overall high degree of primary sequence conservation (86%) relative to the HSV-1 protein, although the carboxy-terminal region which enco...

journal_title：Journal of virology

authors： Greaves RF,O'Hare P

更新日期：1991-12-01 00:00:00

abstract：:Despite intensive research on the identification of T-cell epitopes in cattle after foot-and-mouth disease virus (FMDV) infection during the last 20 years, knowledge of major histocompatibility complex (MHC) restriction and anchor residues of such epitopes is still sparse. Therefore, as a first step, we tested lymphoc...

journal_title：Journal of virology

authors： Gerner W,Hammer SE,Wiesmüller KH,Saalmüller A

更新日期：2009-05-01 00:00:00

abstract：:Cellular annexin II has been shown to specifically bind human cytomegalovirus (HCMV) and be a component of highly purified virions. In this report, we characterize the interaction of annexin II with HCMV. We found that the binding of annexin II to the HCMV envelope occurs partially through the calcium-dependent phosph...

journal_title：Journal of virology

authors： Pietropaolo RL,Compton T

更新日期：1997-12-01 00:00:00

abstract：:3-O-(3',3'-dimethylsuccinyl)betulinic acid (PA-457 or bevirimat) potently inhibits human immunodeficiency virus type 1 (HIV-1) maturation by blocking a late step in the Gag processing pathway, specifically the cleavage of SP1 from the C terminus of capsid (CA). To gain insights into the mechanism(s) by which HIV-1 cou...

journal_title：Journal of virology

authors： Adamson CS,Ablan SD,Boeras I,Goila-Gaur R,Soheilian F,Nagashima K,Li F,Salzwedel K,Sakalian M,Wild CT,Freed EO

更新日期：2006-11-01 00:00:00

abstract：:The Chinese hamster cell lines E36 and CHOK1 dramatically differ in susceptibility to amphotropic murine leukemia virus (A-MuLV) and gibbon ape leukemia virus (GALV); E36 cells are highly susceptible to both viruses, CHOK1 cells are not. We have previously shown that GALV can infect E36 cells by using both its own rec...

journal_title：Journal of virology

authors： Chaudry GJ,Farrell KB,Ting YT,Schmitz C,Lie SY,Petropoulos CJ,Eiden MV

更新日期：1999-04-01 00:00:00

abstract：:Activation of IκB kinase subunit γ (IKKγ), a key regulator of the classical NF-κB pathway, by the vFLIP protein of Kaposi's sarcoma-associated herpesvirus (KSHV) and the Tax protein of human T cell lymphotropic virus type 1 (HTLV1) is essential for virus-associated cancer. We show that vFLIP and Tax activate this path...

journal_title：Journal of virology

authors： Shimizu A,Baratchian M,Takeuchi Y,Escors D,Macdonald D,Barrett T,Bagneris C,Collins M,Noursadeghi M

更新日期：2011-07-01 00:00:00

abstract：UNLABELLED:Many murine leukemia viruses (MLVs) are partially resistant to restriction by mouse APOBEC3 (mA3) and essentially fully resistant to induction of G-to-A mutations by mA3. In contrast, Vif-deficient HIV-1 (ΔVif HIV-1) is profoundly restricted by mA3, and the restriction includes high levels of G-to-A mutation...

journal_title：Journal of virology

authors： Nair S,Sanchez-Martinez S,Ji X,Rein A

更新日期：2014-04-01 00:00:00

abstract：:Two hundred seventeen field isolates of cucumber mosaic cucumovirus (CMV), sampled from 11 natural populations, were typed by RNase protection assay (RPA) using probes from the genomic RNAs of strains in subgroup I and in subgroup II of CMV strains. Most (85%) of the analyzed isolates belonged to subgroup I. For these...

journal_title：Journal of virology

authors： Fraile A,Alonso-Prados JL,Aranda MA,Bernal JJ,Malpica JM,García-Arenal F

更新日期：1997-02-01 00:00:00

abstract：UNLABELLED:Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and is associated with swollen head syndrome in chickens. Since its discovery in the 1970s, aMPV has been recognized as an economically important pathogen in the pou...

journal_title：Journal of virology

authors： Sun J,Wei Y,Rauf A,Zhang Y,Ma Y,Zhang X,Shilo K,Yu Q,Saif YM,Lu X,Yu L,Li J

更新日期：2014-11-01 00:00:00

abstract：:The gastric mucosa is an important portal of entry for lymphocytic choriomeningitis virus (LCMV) infections. Within hours after intragastric (i.g.) inoculation, virus appears in the gastric epithelia, then in the mesenteric lymph nodes and spleen, and then in the liver and brain. By 72 h i.g.-inoculated virus is widel...

journal_title：Journal of virology

authors： Yin C,Djavani M,Schenkel AR,Schmidt DS,Pauza CD,Salvato MS

更新日期：1998-11-01 00:00:00

abstract：:P-element-induced wimpy-like (Piwil) proteins restrict the replication of mobile genetic elements in the germ line. They are also expressed in many transformed cell lines. In this study, we discovered that the human Piwil 2 (Hili) protein can also inhibit HIV replication, especially in activated CD4+ T cells that are ...

journal_title：Journal of virology

authors： Peterlin BM,Liu P,Wang X,Cary D,Shao W,Leoz M,Hong T,Pan T,Fujinaga K

更新日期：2017-05-12 00:00:00

abstract：:Immunity to human immunodeficiency virus virion-like structures or a polyprotein has been examined after DNA immunization with Rev-independent expression vectors. A Gag-Pol fusion protein stimulated cytotoxic T lymphocyte and antibody responses to Gag and Pol, while a Gag-Pol pseudoparticle did not elicit substantial ...

journal_title：Journal of virology

authors： Huang Y,Kong WP,Nabel GJ

更新日期：2001-05-01 00:00:00

abstract：:The structural instability of inactivated foot-and-mouth disease virus (FMDV) hinders the development of vaccine industry. Here we found that some transition metal ions like Cu2+ and Ni2+ could specifically bind to FMDV capsids at capacities about 7089 and 3448 metal ions per capsid, respectively. These values are abo...

journal_title：Journal of virology

authors： Lin X,Yang Y,Song Y,Li S,Zhang X,Su Z,Zhang S

更新日期：2021-01-13 00:00:00

abstract：:Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced deletions into the 5' noncoding sequence (742 nucleotides long) of the genomes of the Mahoney and Sabin 1 strains of polioviru...

journal_title：Journal of virology

authors： Iizuka N,Kohara M,Hagino-Yamagishi K,Abe S,Komatsu T,Tago K,Arita M,Nomoto A

更新日期：1989-12-01 00:00:00

abstract：:Beta interferon (IFN-beta) expression is triggered by double-stranded RNA, a common intermediate in the replication of many viruses including hepatitis C virus (HCV). The recent development of cell culture-grown HCV allowed us to analyze the IFN signaling pathway following virus infection. In this study, we have exami...

journal_title：Journal of virology

authors： Kanda T,Steele R,Ray R,Ray RB

更新日期：2007-11-01 00:00:00