Abstract:
:A soluble system was developed that could support DNA replication in simian virus 40 (SV40) chromosomes. DNA synthesis in this system required the presence of purified SV40 large tumor antigen, SV40 chromosomes prepared from virus-infected monkey cells, a crude extract from HeLa cells, and several low-molecular-weight components. In comparison to the replication of purified SV40 form I DNA, the rate of DNA synthesis was 15 to 20% in this system. DNA synthesis started near the replication origin of SV40 and proceeded bidirectionally in a semiconservative manner. Micrococcal nuclease digestion experiments revealed that the replicated DNA produced in this system became organized into a regularly spaced array of nucleosome core particles when an appropriate amount of purified HeLa core histones was added to the reaction mixture. SV40 form I DNA replicating under the same conditions was also assembled into nucleosomes, which were arranged in a rather dispersed manner and formed an aberrant chromatin structure.
journal_name
J Viroljournal_title
Journal of virologyauthors
Sugasawa K,Murakami Y,Miyamoto N,Hanaoka F,Ui Mdoi
10.1128/JVI.64.10.4820-4829.1990subject
Has Abstractpub_date
1990-10-01 00:00:00pages
4820-9issue
10eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells. Good clean-out of infectivity from rhinovirus harvests was obtained with the continuous-flow B-V and B-IX rotors. Use of the B-V rotor resulted in the successful concentration of rhinov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.9.937-943.1968
更新日期:1968-09-01 00:00:00
abstract::Serial passage at high multiplicity of pseudorabies virus generates defective interfering particles (DIPs) whose genomes consist at least in part of reiterations of segments of DNA in which sequences originating from different regions of the genome have become covalently linked (F. J. Rixon and T. Ben-Porat, Virology ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.2.318-327.1986
更新日期:1986-08-01 00:00:00
abstract::Hepatitis A virus (HAV) 3C proteinase expressed in Escherichia coli was purified to homogeneity, and its cleavage specificity towards various parts of the viral polyprotein was analyzed. Intermolecular cleavage of the P2-P3 domain of the HAV polyprotein gave rise to proteins 2A, 2B, 2C, 3ABC, and 3D, suggesting that i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1727-1733.1995
更新日期:1995-03-01 00:00:00
abstract::The structural polypeptides of purified Plodia interpunctella granulosis virus were analyzed by three different two-dimensional gel systems. Isoelectric focusing followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis allowed resolution of 53 acidic polypeptides in the enveloped nucleocapsid of the virus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.82-89.1986
更新日期:1986-10-01 00:00:00
abstract:UNLABELLED:We have recently shown in both herpesviruses and phages that packaged viral DNA creates a pressure of tens of atmospheres pushing against the interior capsid wall. For the first time, using differential scanning microcalorimetry, we directly measured the energy powering the release of pressurized DNA from th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01172-15
更新日期:2015-09-01 00:00:00
abstract::BALB/c mice treated with pristane and Abelson virus have been used as an animal model system for the rapid induction of plasmacytomas. Myelomonocytic tumors with helper Moloney murine leukemia virus clonally inserted into the c-myb locus were observed in about 10% of pristane-primed BALB/c mice infected with Abelson v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3721-3725.1987
更新日期:1987-12-01 00:00:00
abstract::The gD, gB, and gH/gL glycoprotein quartet constitutes the basic apparatus for herpes simplex virus (HSV) entry into the cell and fusion. gD serves as a receptor binding glycoprotein and trigger of fusion. The conserved gB and gH/gL execute fusion. Central to understanding HSV entry/fusion has become the dissection of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01287-09
更新日期:2009-10-01 00:00:00
abstract::A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Three monoclonal antibodies localized to site 1B recognized a peptide synthesized to M1 (residues 220 to 236) with enzyme-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3622-3633.1989
更新日期:1989-09-01 00:00:00
abstract::Identifying protease cleavage sites contributes to our understanding of their specificity and biochemical properties and can help in designing specific inhibitors. One route to this end is the generation and screening of random libraries of cleavage sites. Both synthetic and phage-displayed libraries have been extensi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10563-10570.2000
更新日期:2000-11-01 00:00:00
abstract::In view of the concern over potential infection hazards in the use of porcine tissues and organs for xenotransplantation to humans, we investigated the diversity of porcine endogenous retrovirus (PERV) genomes in the DNA of domestic pigs and related species. In addition to the three known envelope subgroups of infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.6.2771-2775.2001
更新日期:2001-03-01 00:00:00
abstract::The mucosal adjuvant effect of synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid [poly(I:C)] against influenza virus was examined under intranasal coadministration with inactivated hemagglutinin (HA) vaccine in BALB/c mice and was shown to have a protective effect against both nasal-restricted infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2910-2919.2005
更新日期:2005-03-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV) DNA fragments were cloned into M13 bacteriophage, and the single-stranded recombinant phage DNAs were used as strand-specific nucleic acid hybridization probes to measure synthesis of plus (genomic) and minus strands of MMTV RNA in cultured cell lines and in cell-free preparations of n...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.1.60-65.1984
更新日期:1984-04-01 00:00:00
abstract::Wild-type Sindbis virus (SINV) strain MRE16 efficiently infects Aedes aegypti midgut epithelial cells (MEC), but laboratory-derived neurovirulent SINV strain TE/5'2J infects MEC poorly. SINV determinants for MEC infection have been localized to the E2 glycoprotein. The E2 amino acid sequences of MRE16 and TE/5'2J diff...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02060-07
更新日期:2008-03-01 00:00:00
abstract::Herpesvirus saimiri (HVS) is the prototype gamma-2 herpesvirus, which naturally infects the squirrel monkey Saimiri sciureus, causing an asymptomatic but persistent infection. The latent phase of gamma-2 herpesviruses is characterized by their ability to persist in a dividing cell population while expressing a limited...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02171-06
更新日期:2007-04-01 00:00:00
abstract::The effect of herpes simplex virus type 2 (HSV-2) infection on the synthesis of DNA in human embryonic fibroblast cells was determined at temperatures permissive (37 C) and nonpermissive (42 C) for virus multiplication. During incubation of HSV-2 infected cultures at 42 C for 2 to 4 days or after shift-down from 42 to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.534-539.1975
更新日期:1975-03-01 00:00:00
abstract::The effect of rifampin on the replication of vaccinia DNA was studied in mouse L cells by a cytochemical techinque and by alkaline sucrose sedimentation analysis of newly synthesized viral DNA molecules. By the use of a fluorescent DNA-binding compound (Hoechst 33258), the sequential appearance, size, and location of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.796-801.1977
更新日期:1977-02-01 00:00:00
abstract:UNLABELLED:Based on an explant reactivation model, it has been proposed that CD8(+) T cells maintain latency in trigeminal ganglia (TG) of mice latently infected with herpes simplex virus 1 (HSV-1) [T. Liu, K. M. Khanna, X. Chen, D. J. Fink, and R. L. Hendricks, J Exp Med 191:1459-1466, 2000, doi:10.1084/jem.191.9.1459...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00255-16
更新日期:2016-04-29 00:00:00
abstract::The morphogenesis of vesicular stomatitis virus was examined using freeze-fracture techniques, and the results obtained were compared with those from previously published experiments carried out with influenza viruses and togaviruses. The process of conversion of the host cell plasma membrane into the vesicular stomat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.601-609.1977
更新日期:1977-02-01 00:00:00
abstract::Rabies continues to present a public health threat in most countries of the world. The most efficient way to prevent and control rabies is to implement vaccination programs for domestic animals. However, traditional inactivated vaccines used in animals are costly and have relatively low efficiency, which impedes their...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02324-16
更新日期:2017-03-13 00:00:00
abstract::Equine herpesvirus 1 (EHV-1, Kentucky A strain) preparations enriched for defective interfering particles (DIPs) can readily establish persistent infection. The UL1 gene, which is conserved in the genome of DIPs that mediate persistent infection, maps between nucleotides 1418 and 2192 (258 amino acids) from the L (lon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4122-4132.1993
更新日期:1993-07-01 00:00:00
abstract::The flavivirus dengue virus (DV) infects cells through a low-pH-triggered membrane fusion reaction mediated by the viral envelope protein E. E is an elongated transmembrane protein with three domains and is organized as a homodimer on the mature virus particle. During fusion, the E protein homodimer dissociates, inser...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00170-10
更新日期:2010-06-01 00:00:00
abstract::ev 2 is one of seven recently described genetic loci of chickens which contain structural genes for endogenous virus. ev 2 is present exclusively in line 72 chickens, an inbred strain of white Leghorns which is homozygous for the capacity to produce Rous-associated virus type 0 (RAV-0), a subgroup E virus. This phenot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.250-255.1980
更新日期:1980-01-01 00:00:00
abstract::Chronic hepatitis B affects over 300 million people who are at risk of developing liver cancer. The basis for the persistence of hepatitis B virus (HBV) in hepatocytes, even in the presence of available antiviral therapies, lies in the accumulation of covalently closed circular DNA (cccDNA) in nuclei of infected cells...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01391-17
更新日期:2017-12-14 00:00:00
abstract::Phosphonoformate (foscarnet) is a pyrophosphate (PP(i)) analogue and a potent inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), acting through the PP(i) binding site on the enzyme. HIV-1 RT can unblock a chain-terminated DNA primer by phosphorolytic transfer of the terminal residue t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.11.6127-6137.2003
更新日期:2003-06-01 00:00:00
abstract::Work in this laboratory previously demonstrated that the tropism of different human immunodeficiency type 1 isolates for infection of human CD4+ continuous cell lines (e.g., T-cell lines and HeLa-CD4 transformants) versus primary macrophages is associated with parallel intrinsic fusogenic specificities of the correspo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5487-5494.1996
更新日期:1996-08-01 00:00:00
abstract::Latency is an integral feature of the pathogenesis of cytomegalovirus infection and disease. Using in situ hybridization, we detected viral DNA in the splenic stroma of mice with acute murine cytomegalovirus (MCMV) infection but could not detect latent infection. By using enzymatic amplification of a 700-bp region of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3330-3334.1991
更新日期:1991-06-01 00:00:00
abstract::We studied the effect of filovirus infection on host cell gene expression by characterizing the regulation of gene expression responses in human liver cells infected with Zaire Ebolavirus (ZEBOV), Reston Ebolavirus (REBOV), and Marburgvirus (MARV), using transcriptional profiling and bioinformatics. Expression microar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.6.3009-3020.2006
更新日期:2006-03-01 00:00:00
abstract::The family Caliciviridae is divided into four genera and consists of single-stranded RNA viruses with hosts ranging from humans to a wide variety of animals. Human caliciviruses are the major cause of outbreaks of acute nonbacterial gastroenteritis, whereas animal caliciviruses cause various host-dependent illnesses w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6469-6479.2004
更新日期:2004-06-01 00:00:00
abstract::The gamma(1)34.5 protein, a virulence factor of herpes simplex viruses, redirects protein phosphatase 1 to dephosphorylate the alpha subunit of translation initiation factor 2 (eIF2alpha). Additionally, it inhibits the induction of antiviral genes by TANK-binding kinase 1. Nevertheless, its precise role in vivo remain...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01431-09
更新日期:2009-12-01 00:00:00
abstract::We have developed a system for producing murine leukemia virus (MLV) pseudotyped with human hepatitis B virus (HBV) large (L) and small (S) surface antigens (HBsAg) for targeting primary human hepatocytes. Using the MLV(HBV) pseudotype virus containing a beta-galactosidase reporter gene, we demonstrated that this pseu...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.76.2.912-917.2002
更新日期:2002-01-01 00:00:00