Abstract:
:Work in this laboratory previously demonstrated that the tropism of different human immunodeficiency type 1 isolates for infection of human CD4+ continuous cell lines (e.g., T-cell lines and HeLa-CD4 transformants) versus primary macrophages is associated with parallel intrinsic fusogenic specificities of the corresponding envelope glycoproteins (Envs). For T-cell line-tropic isolates, it is well established that the target cell must also contain a human-specific fusion cofactor(s) whose identity is unknown. In this study, we tested the hypothesis that the Env fusion specificities underlying T-cell line versus macrophage tropism are determined by distinct cell type-specific fusion cofactors. We applied a recombinant vaccinia virus-based reporter gene assay for Env-CD4-mediated cell fusion; the LAV and Ba-L Envs served as prototypes for T-cell line-tropic and macrophage-tropic isolates, respectively. We examined CD4+ promyeloctic and monocytic cell lines that are infectible by T-cell line-tropic isolates and become susceptible to macrophage-tropic strains only after treatment with differentiating agents. We observed parallel changes in fusion specificity: untreated cells supported fusion by the LAV but not the Ba-L Env, whereas cells treated with differentiating agents acquired fusion competence for Ba-L. These results suggest that in untreated cells, the block to infection by macrophage-tropic isolates is at the level of membrane fusion; furthermore, the differential regulation of fusion permissiveness for the two classes of Envs is consistent with the existence of distinct fusion cofactors. To test this notion directly, we conducted experiments with transient cell hybrids formed between CD4-expressing nonhuman cells (murine NIH 3T3) and different human cell types. Hybrids formed with HeLa cells supported fusion by the LAV Env but not by the Ba-L Env, whereas hybrids formed with primary macrophages showed the opposite specificity; hybrids formed between HeLa cells and macrophages supported fusion by both Envs. These results suggest the existence of cell type-specific fusion cofactors selective for each type of Env, rather than fusion inhibitors for discordant Env-cell combinations. Finally, analyses based on recombinant protein expression and antibody blocking did not support the proposals by others that the CD44 or CD26 antigens are involved directly in the entry of macrophage-tropic isolates.
journal_name
J Viroljournal_title
Journal of virologyauthors
Alkhatib G,Broder CC,Berger EAdoi
10.1128/JVI.70.8.5487-5494.1996subject
Has Abstractpub_date
1996-08-01 00:00:00pages
5487-94issue
8eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::Avian erythroblastosis virus (AEV) RNA rescued from nonproducer cells by superinfection with a helper virus is translated into three polypeptides in the messenger-dependent rabbit reticulocyte lysate. A 75,000 molecular weight polypeptide (P75AEV) is synthesized from 28S RNA and is encoded by the 5' section of the AEV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.1.280-284.1980
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journal_title:Journal of virology
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更新日期:1970-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.76.10.4734-4740.2002
更新日期:2002-05-01 00:00:00
abstract:UNLABELLED:Gammaherpesviruses establish persistent, systemic infections and cause cancers. Murid herpesvirus 4 (MuHV-4) provides a unique window into the early events of host colonization. It spreads via lymph nodes. While dendritic cells (DC) pass MuHV-4 to lymph node B cells, subcapsular sinus macrophages (SSM), whic...
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.79.21.13385-13398.2005
更新日期:2005-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.115-120.1975
更新日期:1975-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.02356-09
更新日期:2010-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1327-1332.2005
更新日期:2005-01-01 00:00:00
abstract::Previous studies with the herpes simplex virus type 1 X type 2 intertypic recombinant RS6 suggested that the genomic region from 0.11 to 0.14 map units is involved in neurovirulence (R. T. Javier, R. L. Thompson, and J. G. Stevens, J. Virol. 61:1978-1984, 1987). To study this further, we isolated an RS6-derived herpes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.4.1381-1387.1988
更新日期:1988-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01255-09
更新日期:2009-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.3.923-927.1978
更新日期:1978-03-01 00:00:00
abstract::Human cytomegalovirus (HCMV) possesses low pathogenic potential in an immunocompetent host. In the immunosuppressed host, however, a wide spectrum of infection outcomes, ranging from asymptomatic to life threatening, can follow either primary or nonprimary infection. The variability in the manifestations of HCMV infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7661-7671.2002
更新日期:2002-08-01 00:00:00
abstract::After entering a host cell, retroviruses such as simian immunodeficiency virus (SIV) uncoat, disassembling the viral capsid. Rates of uncoating that are too high and too low can be detrimental to the efficiency of infection. Rapid uncoating typically leads to blocks in reverse transcription, but the basis for replicat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03239-12
更新日期:2013-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2171-2190.2005
更新日期:2005-02-01 00:00:00
abstract::Co-infection of neonatal BALB/c mice with Friend virus (FV) complex (containing defective spleen focus-forming virus [SFFV] and endogenous N-tropic leukemia-inducing helper virus [LLV-F]) and B-tropic Tennant leukemia virus (TenLV) resulted in the inhibition of LLV-F by the Fv-1(b) gene and recovery of a TenLV pseudot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.523-533.1973
更新日期:1973-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) mutations that confer escape from cytotoxic T-lymphocyte (CTL) recognition can sometimes result in lower viral fitness. These mutations can then revert upon transmission to a new host in the absence of CTL-mediated immune selection pressure restricted by the HLA alleles of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02455-07
更新日期:2008-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.2.499-506.2002
更新日期:2002-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.626-635.1977
更新日期:1977-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.1.41-57.2002
更新日期:2002-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02217-20
更新日期:2021-01-06 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7777-7789.2002
更新日期:2002-08-01 00:00:00
abstract:UNLABELLED:Varicella-zoster virus (VZV) is an alphaherpesvirus that causes varicella and herpes zoster. Membrane fusion is essential for VZV entry and the distinctive syncytium formation in VZV-infected skin and neuronal tissue. Herpesvirus fusion is mediated by a complex of glycoproteins gB and gH-gL, which are necess...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00792-16
更新日期:2016-07-27 00:00:00
abstract::Therapeutic suppression of human immunodeficiency virus type 1 (HIV-1) replication may help elucidate interactions between the host cellular immune responses and HIV-1 infection. We performed a detailed longitudinal evaluation of two subjects before and after the start of highly active antiretroviral therapy (HAART). ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6721-6728.1999
更新日期:1999-08-01 00:00:00
abstract::Interallelic complementation between certain temperature-sensitive mutants of gene 42 of bacteriophage T4 was demonstrated by measuring the incorporation of labeled thymine into DNA. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.2.415-416.1973
更新日期:1973-08-01 00:00:00
abstract::The major obstacle to human immunodeficiency type 1 virus (HIV-1) eradication is a reservoir of latently infected cells that persists despite long-term antiretroviral therapy (ART) and is maintained through cellular proliferation. Long-lived memory CD4+ T cells with high self-renewal capacity, such as central memory (...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2019-12-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00962-10
更新日期:2010-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5526-5540.2001
更新日期:2001-06-01 00:00:00
abstract::The RNA-dependent RNA polymerase (NS5B) of hepatitis C virus (HCV) is an unusually attractive target for drug discovery since it contains five distinct drugable sites. The success of novel antiviral therapies will require nonnucleoside inhibitors to be active in at least patients infected with HCV of subtypes 1a and 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01980-09
更新日期:2010-03-01 00:00:00