Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity.

abstract：:Host cell tRNAs are recruited for use as primers to initiate reverse transcription in retroviruses. Human immunodeficiency virus type 1 (HIV-1) uses tRNA(Lys3) as the replication primer, whereas Rous sarcoma virus (RSV) uses tRNA(Trp). The nucleic acid (NA) chaperone function of the nucleocapsid (NC) domain of HIV-1 G...

journal_title：Journal of virology

authors： Rye-McCurdy TD,Nadaraia-Hoke S,Gudleski-O'Regan N,Flanagan JM,Parent LJ,Musier-Forsyth K

更新日期：2014-07-01 00:00:00

abstract：:DNA polymerase activity was detected in each of eight preparations of concentrated human hepatitis B antigen (HBAg) rich in Dane particles prepared by high-speed centrifugation of antigen-positive human plasma and in none of seven control preparations prepared in the same way from HBAg-negative plasma. The incorporati...

journal_title：Journal of virology

authors： Kaplan PM,Greenman RL,Gerin JL,Purcell RH,Robinson WS

更新日期：1973-11-01 00:00:00

abstract：UNLABELLED:Hepatitis C virus (HCV) requires proteins from the NS3-NS5B polyprotein to create a replicase unit for replication of its genome. The replicase proteins form membranous compartments in cells to facilitate replication, but little is known about their functional organization within these structures. We recentl...

journal_title：Journal of virology

authors： Gomes RG,Isken O,Tautz N,McLauchlan J,McCormick CJ

更新日期：2015-12-30 00:00:00

abstract：:Therapeutic vaccination has the potential to boost immune responses and enhance viral control during chronic infections. However, many therapeutic vaccination approaches have fallen short of expectations, and effective boosting of antiviral T-cell responses is not always observed. To examine these issues, we studied t...

journal_title：Journal of virology

authors： Wherry EJ,Blattman JN,Ahmed R

更新日期：2005-07-01 00:00:00

abstract：:Vaccinia virus has a wide host range and infects mammalian cells of many different species. This suggests that the cell surface receptors for vaccinia virus are ubiquitously expressed and highly conserved. Alternatively, different receptors are used for vaccinia virus infection of different cell types. Here we report ...

journal_title：Journal of virology

authors： Chung CS,Hsiao JC,Chang YS,Chang W

更新日期：1998-02-01 00:00:00

abstract：:Recombinant DNA clones of a viral clone of spleen necrosis virus, an avian retrovirus, were found to have long terminal repeats of different sizes. The variation was in the U3 region of the long terminal repeats, and any one clone had U3 of the same size in both long terminal repeats. The U3 regions in the 5' and 3' l...

journal_title：Journal of virology

authors： Shimotohno K,Temin HM

更新日期：1982-01-01 00:00:00

abstract：:Infection of cells with poliovirus induces a massive intracellular membrane reorganization to form vesicle-like structures where viral RNA replication occurs. The mechanism of membrane remodeling remains unknown, although some observations have implicated components of the cellular secretory and/or autophagy pathways....

journal_title：Journal of virology

authors： Belov GA,Altan-Bonnet N,Kovtunovych G,Jackson CL,Lippincott-Schwartz J,Ehrenfeld E

更新日期：2007-01-01 00:00:00

abstract：:The human cytomegalovirus (HCMV) major immediate-early (IE) proteins share an 85-amino-acid N-terminal domain specified by exons 2 and 3 of the major IE region, UL122-123. We have constructed IE Delta30-77, a recombinant virus that lacks the majority of IE exon 3 and consequently expresses smaller forms of both IE1 72...

journal_title：Journal of virology

authors： White EA,Spector DH

更新日期：2005-06-01 00:00:00

abstract：:Wild-type simian virus 40 A protein (large T antigen) bound to three tandem regions of simian virus 40 DNA. The binding regions were defined by the ability of A protein to protect simian virus 40 DNA from digestion with limited (footprint assay) or excess (fragment assay) amounts of DNase I. At low concentrations, pro...

journal_title：Journal of virology

authors： Wilson VG,Tevethia MJ,Lewton BA,Tegtmeyer P

更新日期：1982-11-01 00:00:00

abstract：:A human endogenous retrovirus-like element (HERV), flanked by long terminal repeats of 502 and 495 nucleotides is inserted into the human pleiotrophin (PTN) gene upstream of the open reading frame. Based on its Glu-tRNA primer binding site specificity and the location within the PTN gene, we named this element HERV-E....

journal_title：Journal of virology

authors： Schulte AM,Wellstein A

更新日期：1998-07-01 00:00:00

abstract：:The replication of many positive-strand RNA viruses [(+)RNA viruses] depends on the cellular protein GBF1, but its role in the replication process is not clear. In uninfected cells, GBF1 activates small GTPases of the Arf family and coordinates multiple steps of membrane metabolism, including functioning of the cellul...

journal_title：Journal of virology

authors： Viktorova EG,Gabaglio S,Meissner JM,Lee E,Moghimi S,Sztul E,Belov GA

更新日期：2019-10-15 00:00:00

abstract：:Vpu is a 16-kDa membrane-associated phosphoprotein that is expressed from the same, singly spliced message as the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein precursor, gp160. Previous studies suggest that Vpu functions in the late stages of viral replication, possibly in virus egression from the...

journal_title：Journal of virology

authors： Geraghty RJ,Panganiban AT

更新日期：1993-07-01 00:00:00

abstract：:ETnII elements are mobile members of the repetitive early transposon family of mouse long terminal repeat (LTR) retroelements and have caused a number of mutations by inserting into genes. ETnII sequences lack retroviral genes, but the recent discovery of related MusD retroviral elements with regions similar to gag, p...

journal_title：Journal of virology

authors： Baust C,Gagnier L,Baillie GJ,Harris MJ,Juriloff DM,Mager DL

更新日期：2003-11-01 00:00:00

abstract：:Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by tra...

journal_title：Journal of virology

authors： Gilbert J,Ou W,Silver J,Benjamin T

更新日期：2006-11-01 00:00:00

abstract：:The RNA genomes from the cardioviruses, hepatoviruses, and aphthoviruses encode two to five tandem pseudoknots within their 5' untranslated regions. These pseudoknots lie adjacent to a pyrimidine-rich sequence, which in cardio- and aphthoviruses takes the form of a homopolymeric poly(C) tract. Seven deletion mutations...

journal_title：Journal of virology

authors： Martin LR,Palmenberg AC

更新日期：1996-11-01 00:00:00

abstract：:Jaagsiekte sheep retrovirus (JSRV) is the causative agent of a transmissible lung cancer of sheep known as ovine pulmonary carcinoma. Recently, we have found that the expression of the JSRV envelope (Env) is sufficient to transform mouse NIH 3T3 cells in classical transformation assays. To further investigate the mech...

journal_title：Journal of virology

authors： Palmarini M,Maeda N,Murgia C,De-Fraja C,Hofacre A,Fan H

更新日期：2001-11-01 00:00:00

abstract：:The VP6 protein of bluetongue virus possesses a number of activities, including nucleoside triphosphatase, RNA binding, and helicase activity (N. Stauber, J. Martinez-Costas, G. Sutton, K. Monastyrskaya, and P. Roy, J. Virol. 71:7220-7226, 1997). Although the enzymatic functions of the protein have been documented, a ...

journal_title：Journal of virology

authors： Kar AK,Roy P

更新日期：2003-11-01 00:00:00

abstract：:Although the envelope gene of human immunodeficiency virus type 1 shows considerable strain variability, cysteine residues of the envelope protein are strongly conserved, suggesting that they are important to the envelope structure. We constructed and analyzed mutants of a biologically active molecular clone of human ...

journal_title：Journal of virology

authors： Tschachler E,Buchow H,Gallo RC,Reitz MS Jr

更新日期：1990-05-01 00:00:00

abstract：:Infection of neonatal mice with lymphocytic choriomeningitis virus (LCMV) strain Armstrong (ARM) results in a lifelong persistent infection. Viral variants (cytotoxic T lymphocyte [CTL] negative, persistence positive [CTL- P+]) can be isolated from the lymphoid tissues of such mice. Adult mice inoculated with these CT...

journal_title：Journal of virology

authors： Evans CF,Borrow P,de la Torre JC,Oldstone MB

更新日期：1994-11-01 00:00:00

abstract：:tsAF8 cells are temperature-sensitive (ts) mutants of BHK-21 cells that arrest at the nonpermissive temperature in the G1 phase of the cell cycle. When made quiescent by serum restriction, they can be stimulated to enter the S phase by 10% serum at 34 degrees C, but not at 40.6 degrees C. Infection by adenovirus type ...

journal_title：Journal of virology

authors： Rossini M,Jonak GJ,Baserga R

更新日期：1981-06-01 00:00:00

abstract：:The purified T-antigen origin binding domain binds site specifically to site II, the central region of the simian virus 40 core origin. However, in the context of full-length T antigen, the origin binding domain interacts poorly with DNA molecules containing just site II. Here we investigate the contributions of addit...

journal_title：Journal of virology

authors： Reese DK,Sreekumar KR,Bullock PA

更新日期：2004-03-01 00:00:00

abstract：:We have used deletion and recombinant long terminal repeat (LTR) mutants to examine enhancer activity differences between LTRs of the nonpathogenic Akv and the thymus lymphomagenic MCF13 murine retroviruses. Deletion mutant analysis revealed that major control regions for MCF13 and Akv LTR enhancer activity were simil...

journal_title：Journal of virology

authors： Hollon T,Yoshimura FK

更新日期：1989-08-01 00:00:00

abstract：:Interferon (IFN) is an important immune system molecule capable of inducing an antiviral state within cells. Herpes simplex virus type 1 (HSV-1) replication is somewhat reduced in tissue culture in the presence of IFN, presumably due to decreased viral transcription. Here, we show mutations that inactivate immediate-e...

journal_title：Journal of virology

authors： Mossman KL,Saffran HA,Smiley JR

更新日期：2000-02-01 00:00:00

abstract：:Poliovirus (PV) is easily transferred to humans orally; however, no rodent model for oral infections has been developed because of the alimentary tract's low sensitivity to the virus. Here we showed that PV is inactivated by the low pH of the gastric contents in mice. The addition of 3% NaHCO3 to the viral inoculum in...

journal_title：Journal of virology

authors： Ohka S,Igarashi H,Nagata N,Sakai M,Koike S,Nochi T,Kiyono H,Nomoto A

更新日期：2007-08-01 00:00:00

abstract：:Bovine leukemia virus (BLV) from either persistently infected bat cells or fetal lamb kidney cells induced rapid syncytium formation in F81 indicator cells. Distinct syncytia were seen within 2 h after inoculation of cells with highly concentrated (500-fold) cell-free BLV preparations and within 4 to 8 h when unconcen...

journal_title：Journal of virology

authors： Graves DC,Jones LV

更新日期：1981-06-01 00:00:00

abstract：:Glycoprotein gIII of pseudorabies virus is a major antigen found in the envelopes of virus particles as well as in and on the surfaces of infected cells. It is not an essential gene product for virus growth in tissue culture. In this report, we provide evidence that, although it is not essential, the gIII protein is r...

journal_title：Journal of virology

authors： Whealy ME,Robbins AK,Enquist LW

更新日期：1988-07-01 00:00:00

abstract：:Antibodies against CCR5, the major coreceptor for human immunodeficiency virus type 1 (HIV-1), may have antiviral potential as viral fusion inhibitors. In this study, we generated a virus-like particle (VLP)-based vaccine that effectively breaks B-cell tolerance and elicits autoantibodies against CCR5 in pig-tailed ma...

journal_title：Journal of virology

authors： Chackerian B,Briglio L,Albert PS,Lowy DR,Schiller JT

更新日期：2004-04-01 00:00:00

abstract：:When influenza virus monoclonal antibody-resistant (mar) mutants are selected by incubation in vitro with excess antibody, 90 to 99% of the mutants are not detectable. This observation may be explained by encapsidation of mar mutant RNAs within phenotypically wild-type envelopes. This phenotypic hiding can be revealed...

journal_title：Journal of virology

authors： Valcárcel J,Ortín J

更新日期：1989-09-01 00:00:00

abstract：:Activated Notch1 (AcN1) alleles cooperate with oncogenes from DNA tumor viruses in transformation of epithelial cells. AcN1 signaling has pleiotropic effects, and suggested oncogenic roles include driving proliferation through cyclin D1 or the generation of resistance to apoptosis on matrix withdrawal through a phosph...

journal_title：Journal of virology

authors： Nair P,Somasundaram K,Krishna S

更新日期：2003-06-01 00:00:00

abstract：:T-cell epitopes on the E2 protein of rubella virus were studied by using 15 overlapping synthetic peptides covering the E2 protein sequence. The most frequently recognized epitopes on E2 were E2-4 (residues 54 to 74), with 5 of 10 tested T-cell lines responding to it. Two CD4+ cytotoxic T-cell cloned isolated from one...

journal_title：Journal of virology

authors： Ou D,Chong P,Choi Y,McVeigh P,Jefferies WA,Koloitis G,Tingle AJ,Gillam S

更新日期：1992-11-01 00:00:00