Abstract:
:The goose parvovirus (GPV) Rep 1 and Rep 2 proteins are encoded by P9-generated mRNAs that are either unspliced or spliced within the rep gene region, respectively. These mRNAs are present in an approximately equal ratio. The translation of Rep 1 was initiated from the first AUG in unspliced P9-generated mRNA; however, this AUG was bypassed in spliced P9-generated RNA and Rep 2 translation initiated predominately at the next initiating AUG downstream. We show that the choice of the site of initiation of translation of GPV Rep-encoding mRNAs is governed both by the splicing process itself and by the nature of the excised intron.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li L,Qiu J,Pintel DJdoi
10.1128/JVI.01255-09subject
Has Abstractpub_date
2009-10-01 00:00:00pages
10264-8issue
19eissn
0022-538Xissn
1098-5514pii
JVI.01255-09journal_volume
83pub_type
杂志文章abstract::Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) proteins are encapsidated by assembling HIV-1 virions and edit viral cDNA in the next round of infection. Using alpha interferon (IFN-α)-treated monocyte-derived macrophages, we show that infrequent editing of HIV-1 reverse transcripts can als...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00775-11
更新日期:2011-12-01 00:00:00
abstract::By marker rescue with cloned herpes simplex virus 2 DNA fragments, we have mapped the temperature-sensitive mutations of a series of herpes simplex virus 2 mutants to a region of the herpes simplex virus 2 genome that lies within or near the coding sequences for the major DNA-binding protein, ICP8. In cells infected w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.332-342.1983
更新日期:1983-01-01 00:00:00
abstract::The secreted cytokine alpha/beta interferon (IFN-alpha/beta) binds its receptor to activate the Jak-STAT signal transduction pathway, leading to formation of the heterotrimeric IFN-stimulated gene factor 3 (ISGF3) transcription complex for induction of IFN-stimulated genes (ISGs) and establishment of an antiviral stat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01787-08
更新日期:2009-03-01 00:00:00
abstract::Although the major component of the prion is believed to be the oligomer of PrP(Sc), little information is available concerning regions on the PrP(Sc) molecule that affect prion infectivity. During the analysis of PrP(Sc) molecules from various prion strains, we found that PrP(Sc) of the Chandler strain showed a uniqu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01740-08
更新日期:2009-04-01 00:00:00
abstract::Activation of IκB kinase subunit γ (IKKγ), a key regulator of the classical NF-κB pathway, by the vFLIP protein of Kaposi's sarcoma-associated herpesvirus (KSHV) and the Tax protein of human T cell lymphotropic virus type 1 (HTLV1) is essential for virus-associated cancer. We show that vFLIP and Tax activate this path...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02337-10
更新日期:2011-07-01 00:00:00
abstract::The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein la...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00870-14
更新日期:2014-09-01 00:00:00
abstract::The prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a formidable battle horse for the study of viral immunology, as well as viral persistence and associated diseases. Investigations with LCMV have uncovered basic mechanisms by which viruses avoid elimination by the host adaptive immune response. In ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00555-06
更新日期:2006-09-01 00:00:00
abstract::A significant difference in the recombination rates between human immunodeficiency virus type 1 (HIV-1) and the gammaretroviruses was previously reported, with the former being 10 to 100 times more recombinogenic. It is possible that preferential copackaging of homodimers in the case of gammaretroviruses, like murine ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00273-06
更新日期:2006-07-01 00:00:00
abstract::Two live attenuated single-deletion mutant simian immunodeficiency virus (SIV) constructs, SIV239Deltanef and SIVPBj6.6Deltanef, were tested for their abilities to stimulate protective immunity in macaques. During the immunization period the animals were examined for specific immune responses and virus growth. Each co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1262-1270.1999
更新日期:1999-02-01 00:00:00
abstract:UNLABELLED:Varicella-zoster virus (VZV) is an alphaherpesvirus that causes varicella and herpes zoster. Membrane fusion is essential for VZV entry and the distinctive syncytium formation in VZV-infected skin and neuronal tissue. Herpesvirus fusion is mediated by a complex of glycoproteins gB and gH-gL, which are necess...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00792-16
更新日期:2016-07-27 00:00:00
abstract::Foxp3(+) CD4(+) regulatory T cells (Tregs) represent a highly suppressive T cell subset with well-characterized immunosuppressive effects during immune homeostasis and chronic infections, although the role of these cells in acute viral infections is poorly understood. The present study sought to examine the induction ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05685-11
更新日期:2012-03-01 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is the most important cause of acute respiratory disease in infants. Two major subgroups (A and B) have been identified based on antigenic differences in the attachment G protein. Antigenic variation between and within the subgroups may contribute to reinfections with these vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.9157-9167.2005
更新日期:2005-07-01 00:00:00
abstract::Protein kinase activity is associated with both the extracellular and the occluded forms of Autographa californica nuclear polyhedrosis virus, a baculovirus. Serine and threonine are the predominant amino acids phosphorylated by the kinase activity associated with both viral forms; no phosphotyrosine was detected. The...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.275-278.1983
更新日期:1983-04-01 00:00:00
abstract::Broadly neutralizing antibodies (bNAbs) are being explored for HIV-1 prevention and cure strategies. However, administration of purified bNAbs poses challenges in resource-poor settings, where the HIV-1 disease burden is greatest. In vivo vector-based production of bNAbs represents an alternative strategy. We investig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01925-17
更新日期:2018-03-14 00:00:00
abstract::Activation of microglia and astroglia is seen in many neurodegenerative diseases including prion diseases. Activated glial cells produce cytokines as a protective response against certain pathogens and as part of the host inflammatory response to brain damage. In addition, cytokines might also exacerbate tissue damage...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01413-09
更新日期:2009-11-01 00:00:00
abstract::Mutant recombinant respiratory syncytial viruses (RSV) which cannot express the NS1 and M2-2 proteins, designated rA2DeltaNS1 and rA2DeltaM2-2, respectively, were evaluated as live-attenuated RSV vaccines. The rA2DeltaNS1 virus contains a large deletion that should have the advantageous property of genetic stability d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9317-9321.2000
更新日期:2000-10-01 00:00:00
abstract::Three mRNAs from the murine polyomavirus early region encode the three well-characterized tumor antigens. We report the existence of a fourth alternatively spliced mRNA which encodes a fourth tumor antigen, tiny T antigen, which comprises the amino-terminal domain common to all of the T antigens but is extended by six...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.6068-6074.1997
更新日期:1997-08-01 00:00:00
abstract::Immature retroviral particles are assembled by self-association of the structural polyprotein precursor Gag. During maturation the Gag polyprotein is proteolytically cleaved, yielding mature structural proteins, matrix (MA), capsid (CA), and nucleocapsid (NC), that reassemble into a mature viral particle. Proteolytic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05564-11
更新日期:2012-02-01 00:00:00
abstract::SERINC3 (serine incorporator 3) and SERINC5 are recently identified host cell inhibitors of HIV-1 particle infectivity that are counteracted by the viral pathogenesis factor Nef. Here we confirm that HIV-1 Nef, but not HIV-1 Vpu, antagonizes the particle infectivity restriction of SERINC5. SERINC5 antagonism occurred ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01246-16
更新日期:2016-11-14 00:00:00
abstract::The human polyomavirus JC virus (JCV) is the causative agent of the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML), which is commonly seen in AIDS patients. The bicistronic viral RNA, which is transcribed at the late phase of infection, is responsible for expressing the viral capsid prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02138-05
更新日期:2006-09-01 00:00:00
abstract::Expression of the selectable drug resistance gene in retroviral vectors used for gene therapy can lead to a decreased expression of the gene of interest and may induce a host immune response, resulting in a decreased efficiency of gene therapy. In this study, we demonstrate that high-frequency deletion of direct repea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8837-8842.1999
更新日期:1999-10-01 00:00:00
abstract::TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00451-12
更新日期:2012-05-01 00:00:00
abstract::To identify the domains of Vpr that are involved nuclear localization, we transfected HeLa cells with a panel of expression vectors that encode mutant Vpr protein with deletions or substitutions within putative domains. Immunofluorescence staining of transfected cells revealed that wild-type Vpr was localized predomin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7179-7186.2000
更新日期:2000-08-01 00:00:00
abstract::The enhancer unit present in the retrovirus equine infectious anemia virus (EIAV) was previously shown to contain binding sites for proteins belonging to MDBP, PEA2, AP-1, and ets families. The EIAV ets motif matches the consensus sequence for both PEA3- and PU.1-binding sites. Here, we show by gel shift analysis that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.3885-3890.1993
更新日期:1993-07-01 00:00:00
abstract::The v-rel oncogene encoded by reticuloendotheliosis virus strain T is the acutely transforming member of the Rel/NF-kappaB family of transcription factors. In v-Rel-transformed cells, v-Rel exists as homodimers or heterodimers with the endogenous Rel/NF-kappaB proteins c-Rel, NF-kappaB1, NF-kappaB2, and RelA. To exami...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.4928-4939.2002
更新日期:2002-05-01 00:00:00
abstract::Following the initial detection of viral infection, innate immune responses trigger the induction of numerous interferon-stimulated genes (ISGs) to inhibit virus replication and dissemination. One such ISG encodes cholesterol-25-hydroxylase (CH25H), an enzyme that catalyzes the oxidation of cholesterol to form a solub...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01047-18
更新日期:2018-08-29 00:00:00
abstract::Vesicular stomatitis virus (VSV) vectors that express heterologous antigens have shown promise as vaccines in preclinical studies. The efficacy of VSV-based vaccines can be improved by engineering vectors that enhance innate immune responses. We previously generated a VSV vaccine vector that incorporates two enhancing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02898-13
更新日期:2014-01-01 00:00:00
abstract::We previously demonstrated that chimeric porcine parvovirus-like particles (PPV:VLP) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong CD4(+) and CD8(+) T-cell responses specific for the foreign epitopes. In the present study, we investigated the immunogenicity...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2739-2744.1999
更新日期:1999-04-01 00:00:00
abstract::A virus (M-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus RD-114. Immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between M-7 and RD-114. Anti-RD-114 DNA polymerase IgG inhibits M-7 polymerase by 57% compared to 97% for RD-114. M-7 vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.1.133-138.1974
更新日期:1974-07-01 00:00:00
abstract::The largest tegument protein of herpes simplex virus 1 (HSV-1), UL36, contains a novel deubiquitinating activity embedded in it. All members of the Herpesviridae contain a homologue of HSV-1 UL36, the N-terminal segments of which show perfect conservation of those residues implicated in catalysis. For murine cytomegal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15582-15585.2005
更新日期:2005-12-01 00:00:00