Abstract:
:The human polyomavirus JC virus (JCV) is the causative agent of the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML), which is commonly seen in AIDS patients. The bicistronic viral RNA, which is transcribed at the late phase of infection, is responsible for expressing the viral capsid proteins and a small regulatory protein, agnoprotein. Immunohistochemical analysis of brain tissue from subjects with AIDS/PML revealed colocalization of the human immunodeficiency virus type 1 (HIV-1) transactivator, Tat, and JCV agnoprotein in nucleus and cytoplasm of "bizarre" astrocytes. In accord with this observation, we detected the copresence of agnoprotein and Tat in human astrocytes upon infection with JCV and HIV-1 or in astrocytic cells expressing these proteins after transfection. Interestingly, results from infection of human astrocytes with HIV-1 and JCV showed a decrease in the level of HIV-1 replication in cells that are coinfected with JCV. Conversely, a slight increase in the level of JCV replication was observed in the presence of HIV-1. The copresence of JCV and HIV-1 in astrocytes prompted us to investigate the possible cross-interaction of agnoprotein with Tat and its impact on HIV-1 gene transcription. Our results demonstrate that agnoprotein through its N-terminal domain associates with Tat and the interaction causes the suppression of Tat-mediated enhancement of HIV-1 promoter activity in these cells. Results from RNA and protein binding assays showed that agnoprotein can inhibit the association of Tat with its target RNA sequence, TAR, and with cyclin T1. Furthermore, agnoprotein is able to interfere with cross-interaction of Tat with the p65 subunit of NF-kappaB and Sp1, whose functions are critical for Tat activation of the long terminal repeat. These observations unravel a new pathway for the molecular interaction of these two viruses in biologically relevant cells in the brains of AIDS/PML patients.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kaniowska D,Kaminski R,Amini S,Radhakrishnan S,Rappaport J,Johnson E,Khalili K,Del Valle L,Darbinyan Adoi
10.1128/JVI.02138-05subject
Has Abstractpub_date
2006-09-01 00:00:00pages
9288-99issue
18eissn
0022-538Xissn
1098-5514pii
80/18/9288journal_volume
80pub_type
杂志文章abstract::We have developed a high-throughput sequencing (HTS) workflow for investigating paramyxovirus transcription and replication. We show that sequencing of oligo(dT)-selected polyadenylated mRNAs, without considering the orientation of the RNAs from which they had been generated, cannot accurately be used to analyze the a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00571-19
更新日期:2019-08-13 00:00:00
abstract::Replication by many positive-strand RNA viruses includes genomic RNA amplification and subgenomic mRNA (sgRNA) transcription. For brome mosaic virus (BMV), both processes occur in virus-induced, membrane-associated compartments, require BMV replication factors 1a and 2a, and use negative-strand RNA3 as a template for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1438-1451.2005
更新日期:2005-02-01 00:00:00
abstract::Vesicular stomatitis virus (VSV) has been shown in laboratory studies to be effective against a variety of tumors, including malignant brain tumors. However, attenuation of VSV may be necessary to balance the potential toxicity toward normal cells, particularly when targeting brain tumors. Here we compared 10 recombin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02040-09
更新日期:2010-02-01 00:00:00
abstract::A promoter-trap retrovirus has been constructed in which a promoterless polyomavirus middle T antigen gene was inserted in the U3 region of the long terminal repeat of a replication-competent Moloney murine leukemia virus. The resulting virus, designated PyT, was used to infect mouse mammary glands in situ. As expecte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6810-6815.1996
更新日期:1996-10-01 00:00:00
abstract::The hepadnavirus P gene contains amino acid sequences which share homology with all known RNases H. In this study, we made four mutants in which single amino acids of the duck hepatitis B virus (DHBV) RNase H region were altered. In two of them, amino acids at locations comprising the putative catalytic site were chan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6151-6156.1996
更新日期:1996-09-01 00:00:00
abstract::Assembly of hepatitis delta virus (HDV) in infected human hepatocytes involves association of the 1,679- nucleotide single-stranded genomic RNA (deltaRNA) with multiple copies of both small and large forms of the delta protein (deltaAg) to form a ribonucleoprotein particle which in turn interacts with envelope protein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3709-3719.2002
更新日期:2002-04-01 00:00:00
abstract::The simian virus 40 large T antigen is a promiscuous transcriptional activator of many viral and cellular promoters. We show that the promoter structure necessary for T antigen-mediated transcriptional activation is very simple. A TATA or initiator element is required, in addition to an upstream factor-binding site, w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6682-6688.1993
更新日期:1993-11-01 00:00:00
abstract::An mRNA-ribonucleoprotein particle (mRNP) was found in vesicular stomatitis virus (VSV)-infected Chinese hamster ovary cells. The particle was present 3 and 4.5 h after infection but was barely discernible at 2 h. The mRNP (buoyant density, 1.56 g/cm3), which cosedimented with viral nucleocapsid in a sucrose density g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.932-938.1982
更新日期:1982-12-01 00:00:00
abstract::Knowledge of the mechanisms of virus dissemination in acute measles is cursory, but cells of the monocyte/macrophage (MM) lineage appear to be early targets. We characterized the dissemination of the Edmonston B vaccine strain of measles virus (MV-Ed) in peripheral blood mononuclear cells (PBMC) of two mouse strains e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3343-3351.2001
更新日期:2001-04-01 00:00:00
abstract::Three hypervariable regions were identified in a central portion of open reading frame 1 of TT virus DNA, which codes for a putative capsid protein of 770 amino acids. TT virus circulates as quasispecies, with many amino acid substitutions in hypervariable regions, to evade immune surveillance of the hosts and to esta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9604-9608.1999
更新日期:1999-11-01 00:00:00
abstract::Cell cycle progression during cytomegalovirus infection was investigated by fluorescence-activated cell sorter (FACS) analysis of the DNA content in growth-arrested as well as serum-stimulated human fibroblasts. Virus-infected cells maintained in either low (0.2%) or high (10%) serum failed to progress into S phase an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1629-1634.1997
更新日期:1997-02-01 00:00:00
abstract::We have been investigating the first viral genes to be transcribed in Spodoptera frugiperda cells after the infection with Autographa california nuclear polyhedrosis virus in order to identify regulatory proteins required for the activation of early and/or late gene transcription. By screening a cDNA library from poly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.753-758.1993
更新日期:1993-02-01 00:00:00
abstract::Polyacrylamide gel electrophoretic analysis of purified preparations of human and calf diarrhea viruses indicated eight polypeptide components, or possibly nine in the case of the calf diarrhea virus. Thermal denaturation and analytical studies of the calf diarrhea virus genome showed it to consist of 11 double-strand...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.5.1229-1235.1975
更新日期:1975-11-01 00:00:00
abstract::Affinity-purified medium T antigen encoded by NG59, a nontransforming mutant of polyomavirus, is specifically associated with a protein of 72,000 daltons (72K protein). Medium T antigens of wild-type polyomavirus and the transformation-competent mutant dl8 are not associated with the 72K protein. Instead, they form a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.405-410.1987
更新日期:1987-02-01 00:00:00
abstract::We have examined EcoRI-restricted cellular DNA from BALB/c mouse-hamster somatic cell hybrids by blot hybridization for the presence of mouse mammary tumor virus-related sequences. Results of this analysis show that mouse mammary tumor virus-related proviral copies are located on chromosomes 16 (16-kilobase-pair fragm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.1005-1008.1984
更新日期:1984-03-01 00:00:00
abstract::Given the current difficulties generating vaccine-induced neutralizing antibodies to human immunodeficiency virus (HIV), the focus of the vaccine community has shifted toward creating cytotoxic-T-lymphocyte (CTL)-based vaccines. Recent reports of CTL-based vaccine trials in macaques challenged with simian/human immuno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13348-13360.2003
更新日期:2003-12-01 00:00:00
abstract::Physiological microenvironments such as blood, seminal plasma, mucosal secretions, or lymphatic fluids may influence the biology of the virus-host cell and immune interactions for human immunodeficiency virus type 1 (HIV-1). Relative to media, physiological levels of human plasma were found to enhance the infectivity ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6054-6062.1995
更新日期:1995-10-01 00:00:00
abstract::We studied the capacity of active immunization of rhesus monkeys with HIV-1 envelope protein (Env) to induce primary virus cross-reactive neutralizing antibodies to prevent infection following intravenous challenge with simian-human immunodeficiency virus (SHIV). Monkeys were immunized with the human immunodeficiency ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3358-3369.2005
更新日期:2005-03-01 00:00:00
abstract::The Sindbis virus minimal subgenomic mRNA promoter (spanning positions -19 to +5 relative to the subgenomic mRNA start site) is approximately three- to sixfold less active than the fully active -98 to +14 promoter region. We identified two elements flanking the -19 to +5 region which increase its transcription to leve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3509-3519.2001
更新日期:2001-04-01 00:00:00
abstract::MicroRNAs (miRNAs) are key regulators of gene expression in higher eukaryotes. Recently, miRNAs have been identified from viruses with double-stranded DNA genomes. To attempt to identify miRNAs encoded by herpes simplex virus 1 (HSV-1), we applied a computational method to screen the complete genome of HSV-1 for seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00200-06
更新日期:2006-06-01 00:00:00
abstract:UNLABELLED:Influenza A viruses (IAVs) rely on host factors to support their life cycle, as viral proteins hijack or interact with cellular proteins to execute their functions. Identification and understanding of these factors would increase our knowledge of the molecular mechanisms manipulated by the viruses. In this s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02170-14
更新日期:2015-01-01 00:00:00
abstract::Human immunodeficiency virus infection in humans and simian immunodeficiency virus (SIV) infection in rhesus macaques (RM) leads to a generalized loss of immune responses involving perturbations in T-cell receptor (TCR) signaling. In contrast, naturally SIV-infected sooty mangabeys (SM) remain asymptomatic and retain ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11298-11306.2001
更新日期:2001-12-01 00:00:00
abstract::Inhibition of histone deacetylase (HDAC) activity induces growth arrest, differentiation, and, in certain cell types, apoptosis. FR901228, FK228, or depsipeptide, is an HDAC inhibitor effective in T-cell lymphomas. Adult T-cell leukemia (ATL) is caused by human T-cell leukemia virus type 1 (HTLV-1) and remains incurab...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.78.9.4582-4590.2004
更新日期:2004-05-01 00:00:00
abstract::A new group of nucleocytoplasmic shuttling proteins has recently been identified in the structural proteins encoded by several alphaherpesvirus UL47 genes. Nuclear import and export signals for the bovine herpesvirus type 1 UL47 protein (VP8 or bUL47) have been described previously. Here, we study the trafficking of b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01322-06
更新日期:2006-10-01 00:00:00
abstract::Autonomous parvoviruses are thought to uniquely encapsidate single-stranded DNA of minus polarity. In contrast, the defective adeno-associated viruses separately encapsidate equal amounts of plus and minus DNA strands. We reexamined the uniqueness of minus strand encapsidation for the autonomous parvoviruses. Although...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.319-324.1984
更新日期:1984-02-01 00:00:00
abstract::Mammalian cells infected with human adenoviruses (Ads) undergo an apoptotic response as a result of expression of the viral E1A proteins, and this process is suppressed by the viral E1B-19K protein. The intermediary steps in the Ad-induced apoptosis pathway are not fully resolved. The apical step in the canonical mamm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-07
更新日期:2007-10-01 00:00:00
abstract::Recently it has been shown that kaposica, an immune evasion protein of Kaposi's sarcoma-associated herpesvirus, inactivates complement by acting on C3-convertases by accelerating their decay as well as by acting as a cofactor in factor I-mediated inactivation of their subunits C3b and C4b. Here, we have mapped the fun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5850-5856.2005
更新日期:2005-05-01 00:00:00
abstract:UNLABELLED:Type I IFN signaling, which is initiated through activation of the alpha interferon receptor (IFNAR), regulates the expression of proteins that are crucial contributors to immune responses. Paramyxoviruses, including human metapneumovirus (HMPV), have evolved mechanisms to inhibit IFNAR signaling, but the sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03275-14
更新日期:2015-04-01 00:00:00
abstract::The human immunodeficiency virus (HIV) integrase protein (IN) catalyzes two reactions required to integrate HIV DNA into the human genome: 3' processing of the viral DNA ends and integration. IN has three domains, the N-terminal zinc-binding domain, the catalytic core, and the C-terminal SH3 domain. Previously, it was...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3176-3183.1999
更新日期:1999-04-01 00:00:00
abstract::The fundamental event in prion disease is thought to be the posttranslational conversion of the cellular prion protein (PrPC) into a pathogenic isoform (PrPSc). The occurrence of PrPC on the cell surface and PrPSc in amyloid plaques in situ or in aggregates following purification complicates the study of the molecular...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9413-9418.1998
更新日期:1998-11-01 00:00:00