Dissecting the role of the N-terminal domain of human immunodeficiency virus integrase by trans-complementation analysis.

abstract：:Cytotoxic CD8+ T lymphocytes (CTL) directed against the matrix protein pp65 are major effectors in controlling infection against human cytomegalovirus (HCMV), a persistent virus of the Betaherpesvirus family. We previously suggested that cross-presentation of pp65 by nonpermissive dendritic cells (DCs) could overcome ...

journal_title：Journal of virology

authors： Arrode G,Boccaccio C,Abastado JP,Davrinche C

更新日期：2002-01-01 00:00:00

abstract：:Chimeric human immunodeficiency virus type 1 (HIV-1) molecular clones differing only in the envelope V3 region were constructed. The V3 regions were derived from two HIV-1 isolates with a non-syncytium-inducing, non-T-cell-tropic phenotype and from four HIV-1 isolates with a syncytium-inducing, T-cell-tropic phenotype...

journal_title：Journal of virology

authors： de Jong JJ,Goudsmit J,Keulen W,Klaver B,Krone W,Tersmette M,de Ronde A

更新日期：1992-02-01 00:00:00

abstract：:Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan mic...

journal_title：Journal of virology

authors： Pritchard LK,Spencer DI,Royle L,Vasiljevic S,Krumm SA,Doores KJ,Crispin M

更新日期：2015-07-01 00:00:00

abstract：:A panel of seven monoclonal antibodies against the relatively conserved CD4-binding domain on human immunodeficiency virus type 1 (HIV-1) gp120 was generated by immunizing mice with purified gp120. These monoclonal antibodies reacted specifically with gp120 in an enzyme-linked immunosorbent assay and Western blots (im...

journal_title：Journal of virology

authors： Sun NC,Ho DD,Sun CR,Liou RS,Gordon W,Fung MS,Li XL,Ting RC,Lee TH,Chang NT

更新日期：1989-09-01 00:00:00

abstract：:Murine cytomegalovirus (MCMV) proteins m142 and m143 are essential for viral replication. They bind double-stranded RNA and prevent protein kinase R-induced protein synthesis shutoff. Whether the two viral proteins have additional functions such as their homologs in human cytomegalovirus do remained unknown. We show t...

journal_title：Journal of virology

authors： Ostermann E,Warnecke G,Waibler Z,Brune W

更新日期：2015-10-28 00:00:00

abstract：:The 3'-end host-virus junction fragments from two bovine leukemia virus (BLV)-induced lymphoid tumors (tumors 15-4 and 1351), each containing a single provirus, were used as probes to detect large restriction fragments flanking these proviruses. The DNAs from 28 other independent BLV-induced tumors were checked by Sou...

journal_title：Journal of virology

authors： Kettmann R,Deschamps J,Couez D,Claustriaux JJ,Palm R,Burny A

更新日期：1983-07-01 00:00:00

abstract：:The ascomycete Botrytis porri causes clove rot and leaf blight of garlic worldwide. We report here the biological and molecular features of a novel bipartite double-stranded RNA (dsRNA) mycovirus named Botrytis porri RNA virus 1 (BpRV1) from the hypovirulent strain GarlicBc-72 of B. porri. The BpRV1 genome comprises t...

journal_title：Journal of virology

authors： Wu M,Jin F,Zhang J,Yang L,Jiang D,Li G

更新日期：2012-06-01 00:00:00

abstract：:Purified preparations of human adenovirus type 12 showed two bands when subjected to isopycnic centrifugation in a density gradient of cesium chloride. Their density difference was about 0.003 g/ml, suggesting a small difference in their deoxyribonucleic acid to protein ratio. Virions with a lighter density can kill h...

journal_title：Journal of virology

authors： Mak S

更新日期：1971-04-01 00:00:00

abstract：:TMC125 is a potent new investigational nonnucleoside reverse transcriptase inhibitor (NNRTI) that is active against human immunodeficiency virus type 1 (HIV-1) with resistance to currently licensed NNRTIs. Sequential passage experiments with both wild-type virus and NNRTI-resistant virus were performed to identify mut...

journal_title：Journal of virology

authors： Vingerhoets J,Azijn H,Fransen E,De Baere I,Smeulders L,Jochmans D,Andries K,Pauwels R,de Béthune MP

更新日期：2005-10-01 00:00:00

abstract：:Many viruses express inhibitors of programmed cell death (apoptosis), thereby countering host defenses that would otherwise rapidly clear infected cells. To counter this, viruses such as adenoviruses and herpesviruses express recognizable homologs of the mammalian prosurvival protein Bcl-2. In contrast, the majority o...

journal_title：Journal of virology

authors： Okamoto T,Campbell S,Mehta N,Thibault J,Colman PM,Barry M,Huang DC,Kvansakul M

更新日期：2012-11-01 00:00:00

abstract：:This study examines the persistence and fitness of multidrug-resistant (MDR) viruses acquired during primary human immunodeficiency virus infection (PHI). In four individuals, MDR infections persisted over the entire study period, ranging from 36 weeks to 5 years, in the absence of antiretroviral therapy. In stark con...

journal_title：Journal of virology

authors： Brenner BG,Routy JP,Petrella M,Moisi D,Oliveira M,Detorio M,Spira B,Essabag V,Conway B,Lalonde R,Sekaly RP,Wainberg MA

更新日期：2002-02-01 00:00:00

abstract：UNLABELLED:Foamy viruses (FVs) are complex retroviruses that establish lifelong persistent infection without evident pathology. However, the roles of cellular factors in FV latency are poorly understood. This study revealed that N-Myc interactor (Nmi) could inhibit the replication of prototype foamy virus (PFV). Overex...

journal_title：Journal of virology

authors： Hu X,Yang W,Liu R,Geng Y,Qiao W,Tan J

更新日期：2014-06-01 00:00:00

abstract：:The Epstein-Barr virus nuclear antigen 2 (EBNA-2) acidic domain is essential for B-lymphocyte growth transformation and can activate transcription when brought to a promoter by a sequence-specific DNA-binding domain. We now show that the EBNA-2 acidic domain has slightly less activity than the proteotypic acidic trans...

journal_title：Journal of virology

authors： Tong X,Wang F,Thut CJ,Kieff E

更新日期：1995-01-01 00:00:00

abstract：:The foot-and-mouth disease virus (FMDV) capsid protein precursor, P1-2A, is cleaved by 3C(pro) to generate VP0, VP3, VP1, and the peptide 2A. The capsid proteins self-assemble into empty capsid particles or viruses which do not contain 2A. In a cell culture-adapted strain of FMDV (O1 Manisa [Lindholm]), three differen...

journal_title：Journal of virology

authors： Gullberg M,Polacek C,Bøtner A,Belsham GJ

更新日期：2013-11-01 00:00:00

abstract：:The polypeptides of three paramyxoviruses (simian virus 5, Newcastle disease virus, and Sendai virus) were separated by polyacrylamide gel electrophoresis. Glycoproteins were identified by the use of radioactive glucosamine as a carbohydrate precursor. The protein patterns reveal similarities among the three viruses. ...

journal_title：Journal of virology

authors： Mountcastle WE,Compans RW,Choppin PW

更新日期：1971-01-01 00:00:00

abstract：:The retrovirus type B leukemogenic virus (TBLV) causes T-cell lymphomas in mice. We have identified the Rorgamma locus as an integration site in 19% of TBLV-induced tumors. Overexpression of one or more Rorgamma isoforms in >77% of the tumors tested may complement apoptotic effects of c-myc overexpression. ...

journal_title：Journal of virology

authors： Broussard DR,Lozano MM,Dudley JP

更新日期：2004-05-01 00:00:00

abstract：:Detailed phylogenetic analyses were performed to characterize an HIV-1 outbreak among injection drug users (IDUs) in Stockholm, Sweden, in 2006. This study investigated the source and dynamics of HIV-1 spread during the outbreak as well as associated demographic and clinical factors. Seventy Swedish IDUs diagnosed dur...

journal_title：Journal of virology

authors： Skar H,Axelsson M,Berggren I,Thalme A,Gyllensten K,Liitsola K,Brummer-Korvenkontio H,Kivelä P,Spångberg E,Leitner T,Albert J

更新日期：2011-01-01 00:00:00

abstract：:When herpes simplex virus was inoculated into the flank of a BALB/c mouse by scarification, the local replication of virus was followed by the establishment of an acute ganglionic infection. The subsequent centrifugal spread of this virus along nerves to the skin of the whole dermatome led to the development of a band...

journal_title：Journal of virology

authors： Simmons A,Nash AA

更新日期：1985-03-01 00:00:00

abstract：:Corneal infection with herpes simplex virus 1 (HSV-1) leads to infection of trigeminal ganglia (TG), typically followed by the establishment of latency in the infected neurons. When latency is disrupted, the virus reactivates and migrates back to the cornea, where it restimulates the immune response, leading to lesion...

journal_title：Journal of virology

authors： Yin XT,Baugnon NK,Potter CA,Tai S,Keadle TL,Stuart PM

更新日期：2019-07-30 00:00:00

abstract：:Plasmid lambdadv1, which is in a dimeric form, was converted to a linear monomer duplex by the action of EcoRI restriction endonuclease that incises at a unique site in this plasmid genome. The resulting products were then joined by Escherichia coli DNA ligase to produce molecules with various oligomeric forms, and fr...

journal_title：Journal of virology

authors： Matsubara K,Takagi Y,Mukai T

更新日期：1975-09-01 00:00:00

abstract：:Coronavirus subgenomic mRNA possesses a 5'-end leader sequence which is derived from the 5' end of genomic RNA and is linked to the mRNA body sequence. This study examined whether coronavirus transcription involves a discontinuous transcription step; the possibility that a leader sequence from mouse hepatitis virus (M...

journal_title：Journal of virology

authors： Jeong YS,Makino S

更新日期：1994-04-01 00:00:00

abstract：:NK cells are key effectors of innate immunity and host survival during cytomegalovirus (CMV) infection. Innate murine CMV (MCMV) resistance in MA/My mice requires Ly49H/m157-independent H-2k-linked NK cell control. Here we show that replacement of MA/My H-2k with C57L H-2b susceptibility genes led to a remarkable loss...

journal_title：Journal of virology

authors： Xie X,Dighe A,Clark P,Sabastian P,Buss S,Brown MG

更新日期：2007-01-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is a herpesvirus commonly associated with several malignancies, particularly in immunocompromised hosts. As a strategy for stimulating immunity against EBV for the treatment of EBV-associated tumors, we have genetically engineered dendritic cells (DC) to express EBV antigens, such as latent me...

journal_title：Journal of virology

authors： Ranieri E,Herr W,Gambotto A,Olson W,Rowe D,Robbins PD,Kierstead LS,Watkins SC,Gesualdo L,Storkus WJ

更新日期：1999-12-01 00:00:00

abstract：:Hepatitis C virus (HCV) enters cells via a pH- and clathrin-dependent endocytic pathway. Scavenger receptor BI (SR-BI) and CD81 are important entry factors for HCV internalization into target cells. The SR-BI gene gives rise to at least two mRNA splice variants, SR-BI and SR-BII, which differ in their C termini. SR-BI...

journal_title：Journal of virology

authors： Grove J,Huby T,Stamataki Z,Vanwolleghem T,Meuleman P,Farquhar M,Schwarz A,Moreau M,Owen JS,Leroux-Roels G,Balfe P,McKeating JA

更新日期：2007-04-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is believed to begin with interactions between cell-free HCV and cell receptors that include CD81, scavenger receptor B1 (SR-B1), claudin-1 (CLDN1), and occludin (OCLN). In this study, we have demonstrated that HCV spreading from infected hepatocytes to uninfected hepatocytes leads to...

journal_title：Journal of virology

authors： Liu Z,He JJ

更新日期：2013-08-01 00:00:00

abstract：:We generated a recombinant influenza A virus (Mmut) that produced low levels of matrix (M1) and M2 proteins in infected cells. Mmut virus propagated to significantly lower titers than did wild-type virus in cells infected at low multiplicity. By contrast, virion morphology and incorporation of viral proteins and vRNAs...

journal_title：Journal of virology

authors： Bourmakina SV,García-Sastre A

更新日期：2005-06-01 00:00:00

abstract：:Complementary DNA clones encoding the murine homolog (mCAR) of the human coxsackievirus and adenovirus receptor (CAR) were isolated. Nonpermissive CHO cells transfected with mCAR cDNA became susceptible to infection by coxsackieviruses B3 and B4 and showed increased susceptibility to adenovirus-mediated gene transfer....

journal_title：Journal of virology

authors： Bergelson JM,Krithivas A,Celi L,Droguett G,Horwitz MS,Wickham T,Crowell RL,Finberg RW

更新日期：1998-01-01 00:00:00

abstract：:Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or H...

journal_title：Journal of virology

authors： Chen SL,Lin YK,Li LY,Tsao YP,Lo HY,Wang WB,Tsai TC

更新日期：1996-12-01 00:00:00

abstract：:We have examined the influence of the V1/V2 region of the human immunodeficiency virus type 1 (HIV-1) gp120 on certain biologic properties of the virus. We observed that on the genomic background of the T-cell-line-tropic strain, HIV-1SF2mc, both the V1 and V2 domains of the macrophage-tropic strain, HIV-1SF162mc, in ...

journal_title：Journal of virology

authors： Koito A,Harrowe G,Levy JA,Cheng-Mayer C

更新日期：1994-04-01 00:00:00

abstract：:Infection of the central nervous system (CNS) by several viruses can lead to upregulation of proinflammatory cytokines and chemokines. In immunocompetent adults, these molecules induce prominent inflammatory infiltrates. However, with immunosuppressive retroviruses, such as human immunodeficiency virus (HIV), little C...

journal_title：Journal of virology

authors： Peterson KE,Robertson SJ,Portis JL,Chesebro B

更新日期：2001-03-01 00:00:00