Processing of the VP1/2A junction is not necessary for production of foot-and-mouth disease virus empty capsids and infectious viruses: characterization of "self-tagged" particles.

Abstract:

:The foot-and-mouth disease virus (FMDV) capsid protein precursor, P1-2A, is cleaved by 3C(pro) to generate VP0, VP3, VP1, and the peptide 2A. The capsid proteins self-assemble into empty capsid particles or viruses which do not contain 2A. In a cell culture-adapted strain of FMDV (O1 Manisa [Lindholm]), three different amino acid substitutions (E83K, S134C, and K210E) were identified within the VP1 region of the P1-2A precursor compared to the field strain (wild type [wt]). Expression of the O1 Manisa P1-2A (wt or with the S134C substitution in VP1) plus 3C(pro), using a transient expression system, resulted in efficient capsid protein production and self-assembly of empty capsid particles. Removal of the 2A peptide from the capsid protein precursor had no effect on capsid protein processing or particle assembly. However, modification of E83K alone abrogated particle assembly with no apparent effect on protein processing. Interestingly, the K210E substitution, close to the VP1/2A junction, completely blocked processing by 3C(pro) at this cleavage site, but efficient assembly of "self-tagged" empty capsid particles, containing the uncleaved VP1-2A, was observed. These self-tagged particles behaved like the unmodified empty capsids in antigen enzyme-linked immunosorbent assays and integrin receptor binding assays. Furthermore, mutant viruses with uncleaved VP1-2A could be rescued in cells from full-length FMDV RNA transcripts encoding the K210E substitution in VP1. Thus, cleavage of the VP1/2A junction is not essential for virus viability. The production of such engineered self-tagged empty capsid particles may facilitate their purification for use as diagnostic reagents and vaccines.

journal_name

J Virol

journal_title

Journal of virology

authors

Gullberg M,Polacek C,Bøtner A,Belsham GJ

doi

10.1128/JVI.01863-13

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

11591-603

issue

21

eissn

0022-538X

issn

1098-5514

pii

JVI.01863-13

journal_volume

87

pub_type

杂志文章
  • Rotavirus infection is not associated with small intestinal fluid secretion in the adult mouse.

    abstract::In contrast to humans, adult but not infant small animals are resistant to rotavirus diarrhea. The pathophysiological mechanism behind this age-restricted diarrhea is currently unresolved, and this question was investigated by studying the secretory state of the small intestines of adult mice infected with rotavirus. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00152-06

    authors: Kordasti S,Istrate C,Banasaz M,Rottenberg M,Sjövall H,Lundgren O,Svensson L

    更新日期:2006-11-01 00:00:00

  • The varicella-zoster virus portal protein is essential for cleavage and packaging of viral DNA.

    abstract::The varicella-zoster virus (VZV) open reading frame 54 (ORF54) gene encodes an 87-kDa monomer that oligomerizes to form the VZV portal protein, pORF54. pORF54 was hypothesized to perform a function similar to that of a previously described herpes simplex virus 1 (HSV-1) homolog, pUL6. pUL6 and the associated viral ter...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00376-14

    authors: Visalli MA,House BL,Selariu A,Zhu H,Visalli RJ

    更新日期:2014-07-01 00:00:00

  • Equine infectious anemia virus and the ubiquitin-proteasome system.

    abstract::Some retroviruses contain monoubiquitinated Gag and do not bud efficiently from cells treated with proteasome inhibitors, suggesting an interaction between the ubiquitin-proteasome system and retrovirus assembly. We examined equine infectious anemia virus (EIAV) particles and found that approximately 2% of the p9(Gag)...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.6.3038-3044.2002

    authors: Ott DE,Coren LV,Sowder RC 2nd,Adams J,Nagashima K,Schubert U

    更新日期:2002-03-01 00:00:00

  • Scrapie-infected murine neuroblastoma cells produce protease-resistant prion proteins.

    abstract::Scrapie and Creutzfeldt-Jakob disease are transmissible, degenerative neurological diseases caused by prions. Considerable evidence argues that prions contain protease-resistant sialoglycoproteins, designated PrPSc, encoded by a cellular gene. The prion protein (PrP) gene also encodes a normal cellular protein designa...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.62.5.1558-1564.1988

    authors: Butler DA,Scott MR,Bockman JM,Borchelt DR,Taraboulos A,Hsiao KK,Kingsbury DT,Prusiner SB

    更新日期:1988-05-01 00:00:00

  • Replication of simian herpesvirus SA8 and identification of viral polypeptides in infected cells.

    abstract::The replication of the simian herpesvirus SA8 in Vero cells was examined. The time course of replication of the simian herpesvirus SA8 was found to be similar to that of the herpes simplex viruses. Infectious progeny virions were first detectable by 6 h postinfection and were readily released into the extracellular fl...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.50.2.316-324.1984

    authors: Eberle R,Hilliard JK

    更新日期:1984-05-01 00:00:00

  • A chimeric vesiculo/alphavirus is an effective alphavirus vaccine.

    abstract::While a large number of mosquito-transmitted alphaviruses are known to cause serious human diseases, there are no licensed vaccines that protect against alphavirus infections. The alphavirus chikungunya virus (CHIKV) has caused multiple recent outbreaks of chikungunya fever. This virus has the potential to cause a wor...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01860-12

    authors: Chattopadhyay A,Wang E,Seymour R,Weaver SC,Rose JK

    更新日期:2013-01-01 00:00:00

  • Promoter-specific transactivation of hepatitis B virus transcription by a glutamine- and proline-rich domain of hepatocyte nuclear factor 1.

    abstract::The cloned transcription factor hepatocyte nuclear factor 1 (HNF1) transactivates transcription from the hepatitis B virus (HBV) large surface antigen promoter but does not influence the transcriptional activities of the other three HBV promoters. This indicates that this transcription factor can differentially influe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.65.11.5774-5781.1991

    authors: Raney AK,Easton AJ,Milich DR,McLachlan A

    更新日期:1991-11-01 00:00:00

  • Studies on the role of the 2'-5'-oligoadenylate synthetase-RNase L pathway in beta interferon-mediated inhibition of encephalomyocarditis virus replication.

    abstract::Interferons inhibit the replication of vesicular stomatitis virus (VSV), but not of encephalomyocarditis virus (EMCV), in mouse JLSV-11 cells. We report the isolation of clonal derivatives from this cell line in which the replication of both viruses is impaired by interferons. These clones were selected from the paren...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.62.9.3175-3181.1988

    authors: Kumar R,Choubey D,Lengyel P,Sen GC

    更新日期:1988-09-01 00:00:00

  • Direct interaction between human cytomegalovirus glycoprotein B and cellular annexin II.

    abstract::Cellular annexin II has been shown to specifically bind human cytomegalovirus (HCMV) and be a component of highly purified virions. In this report, we characterize the interaction of annexin II with HCMV. We found that the binding of annexin II to the HCMV envelope occurs partially through the calcium-dependent phosph...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.12.9803-9807.1997

    authors: Pietropaolo RL,Compton T

    更新日期:1997-12-01 00:00:00

  • The Y-S-L-I tyrosine-based motif in the cytoplasmic domain of the human T-cell leukemia virus type 1 envelope is essential for cell-to-cell transmission.

    abstract::The human T-cell leukemia virus type 1 (HTLV-1) transmembrane glycoprotein has a 24-amino-acid cytoplasmic domain whose function in the viral life cycle is poorly understood. We introduced premature-stop mutations and 18 single-amino-acid substitutions into this domain and studied their effects on cell-to-cell transmi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.73.11.9659-9663.1999

    authors: Delamarre L,Pique C,Rosenberg AR,Blot V,Grange MP,Le Blanc I,Dokhélar MC

    更新日期:1999-11-01 00:00:00

  • Independent isolates of the emerging subgroup J avian leukosis virus derive from a common ancestor.

    abstract::A new subgroup of avian leukosis virus (ALV) that includes a unique env gene, designated J, was identified recently in England. Sequence analysis of prototype English isolate HPRS-103 revealed several other unique genetic characteristics of this strain and provided information that it arose by recombination between ex...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.12.10301-10304.1998

    authors: Benson SJ,Ruis BL,Garbers AL,Fadly AM,Conklin KF

    更新日期:1998-12-01 00:00:00

  • Neutralizing antibodies to an immunodominant envelope sequence do not prevent gp120 binding to CD4.

    abstract::Animals immunized with the human immunodeficiency virus type 1 gp160 glycoprotein or certain recombinant envelope components develop potent virus-neutralizing activity. This activity is principally due to antibodies directed toward a hypervariable region of gp120 between cysteine residues 302 and 337 and is virus isol...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.62.11.4195-4200.1988

    authors: Skinner MA,Langlois AJ,McDanal CB,McDougal JS,Bolognesi DP,Matthews TJ

    更新日期:1988-11-01 00:00:00

  • The domains of glycoprotein D required to block apoptosis induced by herpes simplex virus 1 are largely distinct from those involved in cell-cell fusion and binding to nectin1.

    abstract::Glycoprotein D (gD) interacts with two alternative protein receptors, nectin1 and HveA, to mediate herpes simplex virus (HSV) entry into cells. Fusion of the envelope with the plasma membrane requires, in addition to gD, glycoproteins gB, gH, and gL. Coexpression of the four glycoproteins (gD, gB, gH, and gL) promotes...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.6.3759-3767.2003

    authors: Zhou G,Avitabile E,Campadelli-Fiume G,Roizman B

    更新日期:2003-03-01 00:00:00

  • Thermostabilities of virion activities of Newcastle disease virus: evidence that the temperature-sensitive mutants in complementation groups B, BC, and C have altered HN proteins.

    abstract::Four virion activities of Newcastle disease virus (hemagglutinating, neuraminidase, hemolytic, and infectious activities) were examined before and after heat stress in low-salt buffer and physiological salt buffer (phosphate-buffered saline). The hemagglutinating and neuraminidase activities of the Australia-Victoria ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.45.1.18-26.1983

    authors: Peeples ME,Glickman RL,Bratt MA

    更新日期:1983-01-01 00:00:00

  • Vesicular stomatitis virus evolution during alternation between persistent infection in insect cells and acute infection in mammalian cells is dominated by the persistence phase.

    abstract::Vesicular stomatitis virus has the potential for very rapid evolution in the laboratory, but like many other arboviruses, it evolves at a relatively slow rate in the natural environment. Previous work showed that alternating replication in different cell types does not promote stasis. In order to determine whether oth...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.78.22.12236-12242.2004

    authors: Zárate S,Novella IS

    更新日期:2004-11-01 00:00:00

  • Evolution of the human immunodeficiency virus type 1 subtype-specific V3 domain is confined to a sequence space with a fixed distance to the subtype consensus.

    abstract::Human immunodeficiency virus type 1 (HIV-1) strains can be separated into genetic subtypes based on phylogenetic analysis of the envelope gene. Once it had been shown that population-wide intrasubtype genetic variation of HIV-1 strains increases in the course of the AIDS epidemic, it remained uncertain whether HIV-1 s...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.9.6332-6338.1997

    authors: Lukashov VV,Goudsmit J

    更新日期:1997-09-01 00:00:00

  • Restriction of translation of capped mRNA in vitro as a model for poliovirus-induced inhibition of host cell protein synthesis: relationship to p220 cleavage.

    abstract::Poliovirus infection of HeLa cells results in a rapid inhibition of host protein synthesis by a mechanism that does not affect the translation of poliovirus RNA. It has been suggested that this virus-induced translational control results from inactivation of the cap-binding protein complex, and it has been shown that ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.61.8.2480-2488.1987

    authors: Lloyd RE,Jense HG,Ehrenfeld E

    更新日期:1987-08-01 00:00:00

  • Identification of T-helper epitopes in the VP1 capsid protein of poliovirus.

    abstract::Poliovirus-specific T lymphocytes were isolated from virus-immunized mice of different H-2 haplotypes. Immunological characterization of this population indicates that the effector population involved in the observed poliovirus-specific proliferative response was that of CD4-positive T-helper cells. Proliferative resp...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.5.3042-3047.1992

    authors: Kutubuddin M,Simons J,Chow M

    更新日期:1992-05-01 00:00:00

  • Posttranslational modification and intracellular transport of mumps virus glycoproteins.

    abstract::Analysis of the pronase-derived glycopeptides of isolated mumps virus glycoproteins revealed the presence of both complex and high-mannose-type oligosaccharides on the HN and F1 glycoproteins, whereas only high-mannose-type glycopeptides were detected on F2. Endoglycosidase F, a newly described glycosidase that cleave...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.47.2.354-362.1983

    authors: Herrler G,Compans RW

    更新日期:1983-08-01 00:00:00

  • Structural proteins of mammalian oncogenic RNA viruses: murine leukemia virus neutralization by antisera prepared against purified envelope glycoprotein.

    abstract::Goat and rabbit antisera prepared against a purified Rauscher murine leukemia virus glycoprotein (gp69/71) rapidly neutralized spleen focus-forming virus in Rauscher and Friend virus preparations. Absorption studies revealed that most of the neutralizing activity of goat anti-Rauscher virus gp69/71 serum was directed ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.14.1.187-189.1974

    authors: Steeves RA,Strand M,August JT

    更新日期:1974-07-01 00:00:00

  • Persistent infection of a rat nephroma cell line with kilham rat virus.

    abstract::A rat nephroma cell line was found to be a carrier culture of the Kilham rat virus. Infectious virus was demonstrable only by passage of culture materials into other host systems. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.4.3.313-314.1969

    authors: Wozniak J,Hetrick F

    更新日期:1969-09-01 00:00:00

  • Bovine leukemia virus SU protein interacts with zinc, and mutations within two interacting regions differently affect viral fusion and infectivity in vivo.

    abstract::Bovine leukemia virus (BLV) and human T-cell lymphotropic virus type 1 (HTLV-1) belong to the genus of deltaretroviruses. Their entry into the host cell is supposed to be mediated by interactions of the extracellular (SU) envelope glycoproteins with cellular receptors. To gain insight into the mechanisms governing thi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.16.7956-7967.2002

    authors: Gatot JS,Callebaut I,Van Lint C,Demonté D,Kerkhofs P,Portetelle D,Burny A,Willems L,Kettmann R

    更新日期:2002-08-01 00:00:00

  • At least two types of control regions can be found among naturally occurring BK virus strains.

    abstract::The various strains of BK virus (BKV) exhibit a remarkable degree of heterogeneity in the transcriptional control region, which may affect the biological characteristics of a BKV strain. We describe the detection and sequencing of BKV control regions directly from urine samples and after propagation in cell culture. A...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.64.8.3864-3871.1990

    authors: Sundsfjord A,Johansen T,Flaegstad T,Moens U,Villand P,Subramani S,Traavik T

    更新日期:1990-08-01 00:00:00

  • The members of an Epstein-Barr virus microRNA cluster cooperate to transform B lymphocytes.

    abstract::Epstein-Barr virus (EBV) transforms B lymphocytes through the expression of the latent viral proteins EBNA and latent membrane protein (LMP). Recently, it has become apparent that microRNAs (miRNAs) also contribute to EBV's oncogenic properties; recombinant EBVs that lack the BHRF1 miRNA cluster display a reduced abil...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.05100-11

    authors: Feederle R,Haar J,Bernhardt K,Linnstaedt SD,Bannert H,Lips H,Cullen BR,Delecluse HJ

    更新日期:2011-10-01 00:00:00

  • Sequence diversity in S1 genes and S1 translation products of 11 serotype 3 reovirus strains.

    abstract::The S1 gene nucleotide sequences of 10 type 3 (T3) reovirus strains were determined and compared with the T3 prototype Dearing strain in order to study sequence diversity in strains of a single reovirus serotype and to learn more about structure-function relationships of the two S1 translation products, sigma 1 and si...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.64.10.4842-4850.1990

    authors: Dermody TS,Nibert ML,Bassel-Duby R,Fields BN

    更新日期:1990-10-01 00:00:00

  • Design and intracellular activity of a human single-chain antibody to human immunodeficiency virus type 1 conserved gp41 epitope.

    abstract::A human lymphoid cell line (F172-D8) excreting a human immunodeficiency virus type 1 (HIV-1) anti-gp41 monoclonal antibody was used to construct a plasmid containing the cDNA of the single-chain variable fragment (scFvD8) corresponding to this antibody. A stable human osteosarcoma cell line was obtained which expresse...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.12.5712-5715.2000

    authors: Legastelois I,Desgranges C

    更新日期:2000-06-01 00:00:00

  • Characterization of the genome of cytoplasmic polyhedrosis virus.

    abstract::It is possible to oxidize in situ the 3'-terminals of the double-stranded ribonucleic acid (RNA) contained in structurally intact cytoplasmic polyhedrosis virus. The number of 3'-terminals subsequently found by reduction with tritiated sodium borohydride was equivalent to the number observed in RNA first isolated from...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.7.4.434-437.1971

    authors: Lewandowski LJ,Millward S

    更新日期:1971-04-01 00:00:00

  • Interleukin-7 in plasma correlates with CD4 T-cell depletion and may be associated with emergence of syncytium-inducing variants in human immunodeficiency virus type 1-positive individuals.

    abstract::Human immunodeficiency virus type 1 (HIV-1) primary infection is characterized by the use of CCR5 as a coreceptor for viral entry, which is associated with the non-syncytium-inducing (NSI) phenotype in lymphoid cells. Syncytium-inducing (SI) variants of HIV-1 appear in advanced stages of HIV-1 infection and are charac...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.21.10319-10325.2001

    authors: Llano A,Barretina J,Gutiérrez A,Blanco J,Cabrera C,Clotet B,Esté JA

    更新日期:2001-11-01 00:00:00

  • Protection of chimpanzees from type B hepatitis by immunization with woodchuck hepatitis virus surface antigen.

    abstract::Two chimpanzees immunized with woodchuck hepatitis virus (WHV) surface antigen (WHsAg) developed antibodies cross-reactive with hepatitis B virus (HBV) surface antigen (HBsAg). After challenge with HBV, one animal was completely protected and the other experienced a subclinical infection, without evidence of liver dis...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.60.3.895-901.1986

    authors: Cote PJ,Shapiro M,Engle RE,Popper H,Purcell RH,Gerin JL

    更新日期:1986-12-01 00:00:00

  • Time Course Analysis and Mapping of Autographa californica Nuclear Polyhedrosis Virus Transcripts.

    abstract::To study the expression of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome, intracellular virus-specific proteins and mRNAs were pulsed-labeled, extracted, and analyzed at 6-h intervals during the replicative cycle. Most RNAs were detected between 12 and 24 h postinfection (p.i.), but many continu...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.50.3.739-747.1984

    authors: Rohel DZ,Faulkner P

    更新日期:1984-06-01 00:00:00