Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses.

abstract：:Andes virus (ANDV) causes a fatal hantavirus pulmonary syndrome (HPS) in humans and Syrian hamsters. Human alpha(v)beta(3) integrins are receptors for several pathogenic hantaviruses, and the function of alpha(v)beta(3) integrins on endothelial cells suggests a role for alpha(v)beta(3) in hantavirus directed vascular ...

journal_title：Journal of virology

authors： Matthys VS,Gorbunova EE,Gavrilovskaya IN,Mackow ER

更新日期：2010-01-01 00:00:00

abstract：:The roles of intracellular naked (INV), cell-associated enveloped (CEV), and extracellular enveloped (EEV) forms of vaccinia virus in cell-to-cell and longer-range spread were investigated by using two closely related strains of vaccinia virus, WR and IHD-J. We confirmed previous results that WR and IHD-J produced sim...

journal_title：Journal of virology

authors： Blasco R,Moss B

更新日期：1992-07-01 00:00:00

abstract：:Feline leukemia viruses (FeLVs) belonging to interference subgroup C induce fatal anemia resembling human pure red cell aplasia (PRCA). Subgroup A FeLVs, although closely related genetically to FeLVs of subgroup C, do not induce PRCA. The determinants for PRCA induction by a molecularly cloned prototype subgroup C vir...

journal_title：Journal of virology

authors： Dean GA,Groshek PM,Mullins JI,Hoover EA

更新日期：1992-09-01 00:00:00

abstract：:The promoter of the major immediate-early (MIE) genes of human cytomegalovirus (HCMV), also referred to as the CMV promoter, possesses a cis-acting element positioned downstream of the TATA box between positions -14 and -1 relative to the transcription start site (+1). We determined the role of the cis-acting element ...

journal_title：Journal of virology

authors： Isomura H,Stinski MF,Kudoh A,Nakayama S,Murata T,Sato Y,Iwahori S,Tsurumi T

更新日期：2008-01-01 00:00:00

abstract：:The STP-C488 open reading frame of herpesvirus saimiri encodes an oncoprotein that has transforming and tumor-inducing activities independent of the rest of the herpesvirus genome. STP-C488 protein has an unusual, membrane-associated, fibrous structure and is located primarily in perinuclear compartments. We now repor...

journal_title：Journal of virology

authors： Jung JU,Desrosiers RC

更新日期：1992-03-01 00:00:00

abstract：:Herpes simplex virus 1 replication initiates angiogenesis and inflammation in the cornea. This can result in herpetic stromal keratitis (HSK), which is a leading cause of infection-induced corneal blindness. Host cellular factors mediate the progression of HSK, but little is known about these cellular factors and thei...

journal_title：Journal of virology

authors： Yao HW,Chen SH,Li C,Tung YY,Chen SH

更新日期：2012-08-01 00:00:00

abstract：:A large percentage of the repetitive elements in mammalian genomes are retroelements, which have been moved primarily by LINE-1 retrotransposons and endogenous retroviruses. Although LINE-1 elements have remained active throughout the mammalian radiation, specific groups of endogenous retroviruses generally remain act...

journal_title：Journal of virology

authors： Cantrell MA,Ederer MM,Erickson IK,Swier VJ,Baker RJ,Wichman HA

更新日期：2005-12-01 00:00:00

abstract：:We molecularly cloned the src coding region of tsNY68, a mutant of Rous sarcoma virus temperature sensitive (ts) for transformation, and constructed a series of ts wild-type recombinant src genes. DNA containing the hybrid genes was transfected into chicken cells together with viral vector DNA and helper viral DNA, an...

journal_title：Journal of virology

authors： Nishizawa M,Mayer BJ,Takeya T,Yamamoto T,Toyoshima K,Hanafusa H,Kawai S

更新日期：1985-12-01 00:00:00

abstract：:An alternatively spliced form of c-myb exists that encodes an additional 120 amino acids in chicken and 121 amino acids in human and mouse. These amino acids are encoded by an additional exon, termed exon 9A. This exon is not present in v-myb, and proteins containing these amino acids have never been tested for oncoge...

journal_title：Journal of virology

authors： Woo CH,Sopchak L,Lipsick JS

更新日期：1998-08-01 00:00:00

abstract：:Nef, an accessory protein of human and simian immunodeficiency viruses, is a critical determinant of pathogenesis that promotes the progression from infection to AIDS. The pathogenic effects of Nef are in large part dependent on its ability to downregulate the macrophage and T-cell coreceptor, CD4. It has been propose...

journal_title：Journal of virology

authors： Chaudhuri R,Lindwasser OW,Smith WJ,Hurley JH,Bonifacino JS

更新日期：2007-04-01 00:00:00

abstract：:The neutralizing activities of polyclonal antibodies and monoclonal antibodies (MAbs) obtained by immunization of mice with L1 virus-like particles (VLPs) were investigated by using pseudovirion infectivity assays for human papillomavirus type 16 (HPV-16), HPV-31, HPV-33, HPV-45, HPV-58, and HPV-59 to obtain a better ...

journal_title：Journal of virology

authors： Combita AL,Touzé A,Bousarghin L,Christensen ND,Coursaget P

更新日期：2002-07-01 00:00:00

abstract：:A cDNA fragment coding for poliovirus capsid polypeptide VP1 was inserted into a simian virus 40 (SV40) genome in the place of the SV40 VP1 gene and fused in phase to the 3' end of the VP2-VP3 genes. Simian cells were infected with the resulting hybrid virus in the presence of an early SV40 mutant used as a helper. In...

journal_title：Journal of virology

authors： Wychowski C,Benichou D,Girard M

更新日期：1987-12-01 00:00:00

abstract：:JC virus (JCV), along with other members of the polyomavirus family, encodes a class of highly conserved proteins, T antigens, that are capable of inducing aneuploidy in cultured cells. We have previously isolated T-antigen DNA variants of JCV from both colon cancer tissues and the corresponding nonneoplastic gastroin...

journal_title：Journal of virology

authors： Ricciardiello L,Chang DK,Laghi L,Goel A,Chang CL,Boland CR

更新日期：2001-02-01 00:00:00

abstract：:Antibodies directed to Sindbis virus (SV) envelope protein E2 are able to control virus replication in vivo and in persistently infected cultures of neurons in vitro. We investigated the mechanisms by which anti-E2 monoclonal antibody (MAb) alters virus replication by using AT3 rat prostatic carcinoma cells expressing...

journal_title：Journal of virology

authors： Després P,Griffin JW,Griffin DE

更新日期：1995-11-01 00:00:00

abstract：:The dynamics of human immunodeficiency virus type 1 (HIV-1) transcription was analyzed in vitro and in vivo by using a specific molecular approach which allows accurate quantitation of the different classes of viral mRNAs. Unspliced (US) and multiply spliced (MS) HIV-1 transcripts were assayed by competitive reverse t...

journal_title：Journal of virology

authors： Bagnarelli P,Valenza A,Menzo S,Sampaolesi R,Varaldo PE,Butini L,Montroni M,Perno CF,Aquaro S,Mathez D,Leibowitch J,Balotta C,Clementi M

更新日期：1996-11-01 00:00:00

abstract：:Retroviruses and retrotransposons insert into the host genome with no obvious sequence specificity. We examined the target sites of the retroelement ZAM by sequencing each host-ZAM junction in the genome of Drosophila melanogaster. Our overall data provide compelling evidence that ZAM integration machinery recognizes ...

journal_title：Journal of virology

authors： Leblanc P,Dastugue B,Vaury C

更新日期：1999-08-01 00:00:00

abstract：:Expression of the hepatitis B virus core antigen (HBcAg) in mouse NIH 3T3 fibroblasts has been shown previously (A. McLachlan et al., J. Virol. 61:683-692, 1987) to result in the nuclear localization of this polypeptide. Since the carboxyl terminus of HBcAg contains four clusters of arginine residues which resemble nu...

journal_title：Journal of virology

authors： Eckhardt SG,Milich DR,McLachlan A

更新日期：1991-02-01 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is consistently found in Kaposi's sarcoma lesions and in body-cavity-based lymphomas. A 17-kb KSHV lambda clone was obtained directly from a Kaposi's sarcoma lesion. DNA sequence analysis of this clone identified an open reading frame whi...

journal_title：Journal of virology

authors： Li M,Lee H,Yoon DW,Albrecht JC,Fleckenstein B,Neipel F,Jung JU

更新日期：1997-03-01 00:00:00

abstract：:Because immunologic classification of human immunodeficiency virus type 1 (HIV) might be more relevant than genotypic classification for designing polyvalent vaccines, studies were undertaken to determine whether immunologically defined groups of HIV ("immunotypes") could be identified. For these experiments, the V3 r...

journal_title：Journal of virology

authors： Zolla-Pazner S,Gorny MK,Nyambi PN,VanCott TC,Nádas A

更新日期：1999-05-01 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) entry inhibitor eCD4-Ig is a fusion of CD4-Ig and a coreceptor-mimetic peptide. eCD4-Ig is markedly more potent than CD4-Ig, with neutralization efficiencies approaching those of HIV-1 broadly neutralizing antibodies (bNAbs). However, unlike bNAbs, eCD4-Ig neutralized al...

journal_title：Journal of virology

authors： Fetzer I,Gardner MR,Davis-Gardner ME,Prasad NR,Alfant B,Weber JA,Farzan M

更新日期：2018-05-29 00:00:00

abstract：:We analyzed control sequences of the human papovavirus JC virus (JCV) to define the cis-acting elements that regulate specific expression of the viral early region genes in glial cells. Nuclear run-on transcription, S1 analysis, and chloramphenicol acetyltransferase enzyme activity in a transient transfection assay es...

journal_title：Journal of virology

authors： Tada H,Lashgari M,Rappaport J,Khalili K

更新日期：1989-01-01 00:00:00

abstract：:The genome of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 rescued by passage on transformed permissive monkey lines (see accompanying paper [Y. Gluzman et al., J. Virol. 24:534-540, 1977]) was analyzed by restriction endonuclease cleavage mapping to obtain biochemical evidence that the rescue o...

journal_title：Journal of virology

authors： Vogel T,Gluzman Y,Winocour E

更新日期：1977-11-01 00:00:00

abstract：:Two hundred seventeen field isolates of cucumber mosaic cucumovirus (CMV), sampled from 11 natural populations, were typed by RNase protection assay (RPA) using probes from the genomic RNAs of strains in subgroup I and in subgroup II of CMV strains. Most (85%) of the analyzed isolates belonged to subgroup I. For these...

journal_title：Journal of virology

authors： Fraile A,Alonso-Prados JL,Aranda MA,Bernal JJ,Malpica JM,García-Arenal F

更新日期：1997-02-01 00:00:00

abstract：:During lytic infection, herpes simplex virus subverts the host cell RNA polymerase II transcription machinery to efficiently express its own genome while repressing the expression of most cellular genes. The mechanism by which RNA polymerase II is directed to the viral delayed-early and late genes is still unresolved....

journal_title：Journal of virology

authors： Rice SA,Long MC,Lam V,Spencer CA

更新日期：1994-02-01 00:00:00

abstract：:China is facing a rapid upsurge in cases of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) infection due to large numbers of paid blood donors (PBD), injection drug users (IDU), and sexual partners of infected individuals. In this report, a total of 236 HIV-1-positive blood samples were collec...

journal_title：Journal of virology

authors： Zhang L,Chen Z,Cao Y,Yu J,Li G,Yu W,Yin N,Mei S,Li L,Balfe P,He T,Ba L,Zhang F,Lin HH,Yuen MF,Lai CL,Ho DD

更新日期：2004-12-01 00:00:00

abstract：:Ribosomes are observed intimately associated with nucleocapsids of vesicular, stomatitis virus, especially those that line structures that are either cytoplasmic vesicles or invaginations of the plasma membrane. ...

journal_title：Journal of virology

authors： Orenstein J,Shelton E,Lazzarini RA

更新日期：1975-08-01 00:00:00

abstract：:The human foamy virus (HFV) contains within the U3 region of its long terminal repeat (LTR) three perfect consensus sequences for the binding of the inducible transcription factor AP-1. Results of DNase I footprint protection and gel retardation assays demonstrated that proteins in extracts of HeLa and BHK-21 cells as...

journal_title：Journal of virology

authors： Maurer B,Serfling E,ter Meulen V,Rethwilm A

更新日期：1991-11-01 00:00:00

abstract：:Organotypic cultures support the stratification and differentiation of keratinocytes and the human papillomavirus (HPV) life cycle. We report transcription from four novel promoters in the HPV31b upstream regulatory region during the viral life cycle in organotypic cultures. Promoter initiation was not differentiation...

journal_title：Journal of virology

authors： Ozbun MA,Meyers C

更新日期：1999-04-01 00:00:00

abstract：UNLABELLED:The porcine sapovirus (SaV) (PoSaV) Cowden strain is one of only a few culturable enteric caliciviruses. Compared to the wild-type (WT) PoSaV Cowden strain, tissue culture-adapted (TC) PoSaV has two conserved amino acid substitutions in the RNA-dependent RNA polymerase (RdRp) and six in the capsid protein (V...

journal_title：Journal of virology

authors： Lu Z,Yokoyama M,Chen N,Oka T,Jung K,Chang KO,Annamalai T,Wang Q,Saif LJ

更新日期：2015-11-18 00:00:00

abstract：:The major inducible 70-kDa heat shock protein (hsp70) is host protective in a mouse model of measles virus (MeV) brain infection. Transgenic constitutive expression of hsp70 in neurons, the primary target of MeV infection, abrogates neurovirulence in neonatal H-2(d) congenic C57BL/6 mice. A significant level of protec...

journal_title：Journal of virology

authors： Kim MY,Shu Y,Carsillo T,Zhang J,Yu L,Peterson C,Longhi S,Girod S,Niewiesk S,Oglesbee M

更新日期：2013-01-01 00:00:00