Abstract:
:Nef, an accessory protein of human and simian immunodeficiency viruses, is a critical determinant of pathogenesis that promotes the progression from infection to AIDS. The pathogenic effects of Nef are in large part dependent on its ability to downregulate the macrophage and T-cell coreceptor, CD4. It has been proposed that Nef induces downregulation by linking the cytosolic tail of CD4 to components of the host-cell protein trafficking machinery. To identify these components, we developed a novel Nef-CD4 downregulation system in Drosophila melanogaster S2 cells. We found that human immunodeficiency virus type 1 (HIV-1) Nef downregulates human CD4 in S2 cells and that this process is subject to the same sequence requirements as in human cells. An RNA interference screen targeting protein trafficking genes in S2 cells revealed a requirement for clathrin and the clathrin-associated, plasma membrane-localized AP2 complex in the downregulation of CD4. The requirement for AP2 was confirmed in the human cell line HeLa. We also used a yeast three-hybrid system and glutathione S-transferase pull-down analyses to demonstrate a robust, direct interaction between HIV-1 Nef and AP2. This interaction requires a dileucine motif in Nef that is also essential for downregulation of CD4. Together, these results support a model in which HIV-1 Nef downregulates CD4 by promoting its accelerated endocytosis by a clathrin/AP2 pathway.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chaudhuri R,Lindwasser OW,Smith WJ,Hurley JH,Bonifacino JSdoi
10.1128/JVI.02725-06subject
Has Abstractpub_date
2007-04-01 00:00:00pages
3877-90issue
8eissn
0022-538Xissn
1098-5514pii
JVI.02725-06journal_volume
81pub_type
杂志文章abstract::Barley stripe mosaic virus (BSMV) encodes three movement proteins in an overlapping triple gene block (TGB), but little is known about the physical interactions of these proteins. We have characterized a ribonucleoprotein (RNP) complex consisting of the TGB1 protein and plus-sense BSMV RNAs from infected barley plants...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02586-07
更新日期:2008-05-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) infection of rhesus macaques causes immune depletion and disease closely resembling human AIDS and is well recognized as the most relevant animal model for the human disease. Experimental investigations of viral pathogenesis and vaccine protection primarily involve a limited set of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01862-12
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abstract::Embryonic stem cells are subjected to a dynamic genome regulation during development. Here we report that the ectopic lentiviral transgenes are quickly silenced in murine embryonic carcinoma P19 cells. The silencing was correlated with CpG hypermethylation in the transgene promoter. Using high-resolution sodium bisulf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13497-13508.2005
更新日期:2005-11-01 00:00:00
abstract::The outer layer of the bacteriophage phi 6 nucleocapsid (NC) was removed by EDTA and reassociated with the core in the presence of Ca2+ or Mg2+. The core was relatively inaccessible to trypsin digestion, was composed of protein P1, and was in the dodecahedral framework reported previously. (H.T. Steely, Jr., and D. La...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2621-2623.1987
更新日期:1987-08-01 00:00:00
abstract::Nucleotide sequence analysis of two simian virus 40 early mutants dl1263 and dl1265, which lack a DNA segment around map positions 0.21 and 0.18, respectively (C. Cole, T. Landers, S. Goff, S. MAnteuil-Brutlag, and P. Berg, J. Virol. 24:277--294, 1977), revealed in-phase deletions of 33 nucleotide pairs for dl1263 and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.3.936-941.1979
更新日期:1979-06-01 00:00:00
abstract::The hepatitis B virus (HBV) X protein (pX) is implicated in hepatocarcinogenesis by an unknown mechanism. pX variants encoded by HBV genomes found integrated in genomic DNA from liver tumors of patients with hepatocellular carcinoma (HCC) generally lack amino acids 134 to 154. Since deregulation of mitogenic pathways ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00661-06
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abstract::Mature dendritic cells (DCs) are potent antigen-presenting cells essential for initiating successful antiviral immune responses and would therefore serve as an ideal target for viruses seeking to evade or delay the immune response by disrupting their function. We have previously reported that VZV productively infects ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4950-4959.2003
更新日期:2003-04-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a herpesvirus commonly associated with several malignancies, particularly in immunocompromised hosts. As a strategy for stimulating immunity against EBV for the treatment of EBV-associated tumors, we have genetically engineered dendritic cells (DC) to express EBV antigens, such as latent me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.10416-10425.1999
更新日期:1999-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6476-6484.2000
更新日期:2000-07-01 00:00:00
abstract::Divergent Toll-like receptor 7 (TLR7) and TLR9 signaling has been proposed to distinguish pathogenic from nonpathogenic simian immunodeficiency virus infection in primate models. We demonstrate here that increased expression of type I interferon in pathogenic rhesus macaques compared to nonpathogenic African green mon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01496-09
更新日期:2010-02-01 00:00:00
abstract::The dynamics of human immunodeficiency virus type 1 (HIV-1) transcription was analyzed in vitro and in vivo by using a specific molecular approach which allows accurate quantitation of the different classes of viral mRNAs. Unspliced (US) and multiply spliced (MS) HIV-1 transcripts were assayed by competitive reverse t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7603-7613.1996
更新日期:1996-11-01 00:00:00
abstract::Bovine viral diarrhea virus (BVDV), a pestivirus of the Flaviviridae family, is an economically important cattle pathogen with a worldwide distribution. Both noncytopathic (ncp) and cytopathic (cp) biotypes of BVDV can be isolated from persistently infected cattle suffering from the lethal mucosal disease. The cp biot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.10.4692-4698.2001
更新日期:2001-05-01 00:00:00
abstract::The mammalian APOBEC3 proteins are an important component of the cellular innate immune response to retroviral infection. APOBEC3G can extinguish HIV-1 infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity that attacks the nascent viral cDNA during reverse transcription, caus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00056-10
更新日期:2010-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00014-20
更新日期:2020-06-16 00:00:00
abstract:UNLABELLED:Venezuelan and western equine encephalitis viruses (VEEV and WEEV; Alphavirus; Togaviridae) are mosquito-borne pathogens causing central nervous system (CNS) disease in humans and equids. Adult CD-1 mice also develop CNS disease after infection with VEEV and WEEV. Adult CD-1 mice infected by the intranasal (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03219-15
更新日期:2016-05-27 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) mutants with codon insertions and deletions in IE-0, the gene encoding ICP0, were constructed. The HSV-1 deletion mutant dl1403 (N. D. Stow and E. C. Stow, J. Gen. Virol. 67:2571-2585, 1986) and an IE-0:lacZ transplacement vector isolated in this study were used to facilitate the co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.2916-2927.1992
更新日期:1992-05-01 00:00:00
abstract::Equine infectious anemia virus (EIAV) is a lentivirus with in vivo cell tropism primarily for tissue macrophages; however, in vitro the virus can be adapted to fibroblasts and other cell types. Tropism adaptation is associated with both envelope and long terminal repeat (LTR) changes, and findings strongly suggest tha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5653-5664.2005
更新日期:2005-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.159-164.1996
更新日期:1996-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9965-9976.2004
更新日期:2004-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4113-4126.2003
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5802-5810.1998
更新日期:1998-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9032-9044.1997
更新日期:1997-12-01 00:00:00
abstract::We have previously described the expression of the major nonstructural protein (NS-1) of Aleutian mink disease parvovirus (ADV) in insect cells by using a baculovirus vector (J. Christensen, T. Storgaard, B. Bloch, S. Alexandersen, and B. Aasted, J. Virol. 67:229-238, 1993). To study its biochemical properties, ADV NS...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1802-1809.1995
更新日期:1995-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00723-08
更新日期:2008-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4433-4440.2000
更新日期:2000-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.16.10788-10795.2005
更新日期:2005-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00858-09
更新日期:2009-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3932-3939.1997
更新日期:1997-05-01 00:00:00
abstract::We have used feline immunodeficiency virus (FIV) protease (PR) as a mutational system to study the molecular basis of substrate-inhibitor specificity for lentivirus PRs, with a focus on human immunodeficiency virus type 1 (HIV-1) PR. Our previous mutagenesis studies demonstrated that discrete substitutions in the acti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00374-06
更新日期:2006-08-01 00:00:00
abstract::By marker rescue with cloned herpes simplex virus 2 DNA fragments, we have mapped the temperature-sensitive mutations of a series of herpes simplex virus 2 mutants to a region of the herpes simplex virus 2 genome that lies within or near the coding sequences for the major DNA-binding protein, ICP8. In cells infected w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.332-342.1983
更新日期:1983-01-01 00:00:00