Abstract:
:Although hepatitis A virus (HAV) is typically transmitted by the fecal-oral route, little is known of its interactions with cells of the gastrointestinal tract. We studied the replication of HAV in polarized cultures of Caco-2 cells, a human cell line which retains many differentiated functions of small intestinal epithelial cells. Virus uptake was 30- to 40-fold more efficient when the inoculum was placed on the apical rather than the basolateral surface of these cells, suggesting a greater abundance of the cellular receptor for HAV on the apical surface. Infection proceeded without cytopathic effect and did not influence transepithelial resistance or the diffusion of inulin across cell monolayers. Nonetheless, there was extensive release of progeny virus, which occurred almost exclusively into apical supernatant fluids (36.4% +/- 12.5% of the total virus yield compared with 0.23% +/- 0.13% release into basolateral fluids). Brefeldin A caused a profound inhibition of HAV replication, but also selectively reduced apical release of virus. These results indicate that polarized human epithelial cell cultures undergo vectorial infection with HAV and that virus release is largely restricted to the apical membrane. Virus release occurs in the absence of cytopathic effect and may involve cellular vesicular transport mechanisms.
journal_name
J Viroljournal_title
Journal of virologyauthors
Blank CA,Anderson DA,Beard M,Lemon SMdoi
10.1128/jvi.74.14.6476-6484.2000subject
Has Abstractpub_date
2000-07-01 00:00:00pages
6476-84issue
14eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3407-3414.1997
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.12.2.397-400.1973
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.492-500.1973
更新日期:1973-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1978-12-01 00:00:00
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更新日期:1982-09-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.5.912-919.1967
更新日期:1967-10-01 00:00:00
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