Abstract:
:Simian virus 40 (SV40) infection of human diploid cells failed to cause an enhanced production of thymidine kinase during the first 10 days after infection. Thymidine kinase activities from extracts of SV40-transformed cultures (human or simian) were considerably higher than the activity levels in extracts from the normal cells of origin. In addition, whereas the kinase activities obtained for human diploid cultures decreased as the cell sheet became confluent, the kinase activities for SV40-transformed human cells remained high after confluence was reached. Antisera obtained from hamsters bearing SV40 or adeno-7-SV40 hybrid virus tumors selectively inhibited enzyme from transformed sources (human or simian). Also, the antisera selectively inhibited enzyme extracted from SV40-lytically infected monkey cells. Sera from normal animals or from hamsters bearing polyoma tumors failed to inhibit enzymes from normal, SV40-transformed, or SV40-lytically infected cells. The Michaelis constant of partially purified enzyme from SV40-transformed cells was two to five times as high as that obtained for partially purified enzyme from human diploid cell cultures.
journal_name
J Viroljournal_title
Journal of virologyauthors
Carp RIdoi
10.1128/JVI.1.5.912-919.1967subject
Has Abstractpub_date
1967-10-01 00:00:00pages
912-9issue
5eissn
0022-538Xissn
1098-5514journal_volume
1pub_type
杂志文章abstract::Noninfectious spikeless particles have been obtained from vesicular stomatitis virus (VSV, Indiana serotype) by bromelain or Pronase treatment. They lack the viral glycoprotein (G) but contain all the other viral components (RNA, lipid, and other structural proteins). Triton-solubilized VSV-Indiana glycoprotein prepar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.75-84.1975
更新日期:1975-07-01 00:00:00
abstract::The innate and adaptive immune responses have evolved distinct strategies for controlling different viral pathogens. Encephalomyocarditis virus (EMCV) is a picornavirus that can cause paralysis, diabetes, and myocarditis within days of infection. The optimal innate immune response against EMCV in vivo requires CD1d. I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02745-05
更新日期:2006-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) sequences were generated from blood and from brain tissue obtained by stereotactic biopsy from six patients undergoing a diagnostic neurosurgical procedure. Proviral DNA was directly amplified by nested PCR, and 8 to 36 clones from each sample were sequenced. Phylogenetic an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7467-7481.1994
更新日期:1994-11-01 00:00:00
abstract::The ability of HIV-1 to establish a latent infection presents a barrier to curing HIV. The best-studied reservoir of latent virus in vivo is resting memory CD4(+) T cells, but it has recently been shown that CD34(+) hematopoietic progenitor cells (HPCs) can also become latently infected by HIV-1 in vitro and in vivo. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00895-12
更新日期:2012-09-01 00:00:00
abstract::The multifunctional simian and human immunodeficiency virus (SIV and HIV) Nef proteins are important for virulence. We studied the importance of selected Nef functions using an SIV Nef with mutations in two regions that are required for CD4 downregulation. This Nef mutant is defective for downregulating CD4 and, in ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.21.9836-9844.2000
更新日期:2000-11-01 00:00:00
abstract:UNLABELLED:The HIV-1 virion infectivity factor (Vif) targets the cellular cytidine deaminases APOBEC3G (A3G) and APOBEC3F (A3F) for degradation via the host ubiquitin-proteasome pathway. Vif recruits a cellular E3 ubiquitin ligase to polyubiquitinate A3G/F. The activity of Vif critically depends on the cellular core bi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03824-13
更新日期:2014-03-01 00:00:00
abstract:UNLABELLED:Although the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) epidemic was controlled by nonvaccine measures, coronaviruses remain a major threat to human health. The design of optimal coronavirus vaccines therefore remains a priority. Such vaccines present major challenges: coronavirus im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02980-14
更新日期:2015-03-01 00:00:00
abstract::The nonstructural protein (NSs) of severe fever with thrombocytopenia syndrome phlebovirus (SFTSV) sequesters TANK-binding kinase 1 (TBK1) into NSs-induced cytoplasmic structures to inhibit the phosphorylation and nuclear translocation of interferon (IFN) regulatory factor 3 (IRF3) and subsequent interferon beta (IFN-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00706-18
更新日期:2018-09-12 00:00:00
abstract::The four major Epstein-Barr virion envelope components were separated by column chromatography and reconstituted into artificial liposomes. These liposomes were tested for their ability to bind selectively to Epstein-Barr virus receptor-positive cells. Only when the two high-molecular-weight glycoproteins, VE1 and VE2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.286-297.1982
更新日期:1982-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1476-1486.2001
更新日期:2001-02-01 00:00:00
abstract::RNA interference (RNAi) is a conserved antiviral immune defense in eukaryotes, and numerous viruses have been found to encode viral suppressors of RNAi (VSRs) to counteract antiviral RNAi. Alphaviruses are a large group of positive-stranded RNA viruses that maintain their transmission and life cycles in both mosquitoe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01233-19
更新日期:2020-01-17 00:00:00
abstract::Certain chicken cells that do not spontaneously release virus particles have been shown to produce a subgroup E avian RNA tumor virus, Rous-associated virus 60 (RAV-60), after infection with viruses of other subgroups. The nucleic acids of RAV-60 were analyzed for sequence homologies with the viral nucleic acids conta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.6.1367-1377.1975
更新日期:1975-06-01 00:00:00
abstract::The efficient attachment of pseudorabies virus to cultured cells is dependent on an electrostatic interaction between negatively charged cell surface heparan sulfate and the viral envelope glycoprotein gC. Deletion of the first one-third of gC severely impairs virus attachment, but the mutant virions are still capable...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.834-839.1995
更新日期:1995-02-01 00:00:00
abstract::Poliovirus-infected cells undergo an increase in cytoplasmic calcium concentrations from the 4th h postinfection. The protein responsible for this effect was identified by the expression of different poliovirus nonstructural proteins in HeLa cells by using a recombinant vaccinia virus system. Synthesis of protein 2BC ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.6214-6217.1997
更新日期:1997-08-01 00:00:00
abstract:UNLABELLED:A balance between the functions of the influenza virus surface proteins hemagglutinin (HA) and neuraminidase (NA) is thought to be important for the transmission of viruses between humans. Here we describe two pandemic H1N1 viruses, A/swine/Virginia/1814-1/2012 and A/swine/Virginia/1814-2/2012 (pH1N1low-1 an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02537-14
更新日期:2015-06-01 00:00:00
abstract::The complete nucleotide sequence of the NS mRNA of vesicular stomatitis virus (New Jersey serotype) was established from two cDNA clones spanning the entire coding region of the mRNA. The gene is 856 nucleotides long and can code for a polypeptide of 274 amino acids. Comparison with the nucleotide sequence of the NS g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.1.60-66.1985
更新日期:1985-07-01 00:00:00
abstract::Retrovirus assembly involves a complex series of events in which a large number of proteins must be targeted to a point on the plasma membrane where immature viruses bud from the cell. Gag polyproteins of most retroviruses assemble an immature capsid on the cytoplasmic side of the plasma membrane during the budding pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.784-795.2000
更新日期:2000-01-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection requires cell surface expression of CD4. Costimulation of CD8(+)/CD4(-) T lymphocytes by anti-CD3 and anti-CD28 antibodies or by allogeneic dendritic cells induced expression of CD4 and rendered these CD8 cells susceptible to HIV-1 infection. Naive CD45RA+ cells re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9054-9060.1998
更新日期:1998-11-01 00:00:00
abstract::Heron hepatitis B virus (HHBV) is an avian hepadnavirus that is closely related to duck hepatitis B virus (DHBV). To learn more about the mechanism of hepadnavirus replication, we characterized a clone of HHBV that contains a substitution of DHBV sequence from nucleotide coordinates 403 to 1364. This clone, named HDE1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8310-8317.1996
更新日期:1996-12-01 00:00:00
abstract::We have developed a system for producing murine leukemia virus (MLV) pseudotyped with human hepatitis B virus (HBV) large (L) and small (S) surface antigens (HBsAg) for targeting primary human hepatocytes. Using the MLV(HBV) pseudotype virus containing a beta-galactosidase reporter gene, we demonstrated that this pseu...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.76.2.912-917.2002
更新日期:2002-01-01 00:00:00
abstract::Infection of neonatal mice with lymphocytic choriomeningitis virus (LCMV) strain Armstrong (ARM) results in a lifelong persistent infection. Viral variants (cytotoxic T lymphocyte [CTL] negative, persistence positive [CTL- P+]) can be isolated from the lymphoid tissues of such mice. Adult mice inoculated with these CT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7367-7373.1994
更新日期:1994-11-01 00:00:00
abstract::T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.1155-1159.1976
更新日期:1976-06-01 00:00:00
abstract::Coronavirus envelope (E) proteins are small (approximately 75- to 110-amino-acid) membrane proteins that have a short hydrophilic amino terminus, a relatively long hydrophobic membrane domain, and a long hydrophilic carboxy-terminal domain. The protein is a minor virion structural component that plays an important, no...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01472-06
更新日期:2007-04-01 00:00:00
abstract::The blood group P antigen, known to be abundantly expressed on erythroid cells, has been reported to be the cellular receptor for parvovirus B19. We have described the development of recombinant parvovirus B19 vectors with which high-efficiency, erythroid lineage-restricted transduction can be achieved (S. Ponnazhagan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4110-4116.2001
更新日期:2001-05-01 00:00:00
abstract::A cloned segment of the polyoma virus genome encoding the small T antigen has been fused, in the correct phase for translation, to the 5' end of the beta-galactosidase gene. The hybrid gene, cloned in Escherichia coli, produces a protein resembling the small T antigen. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.1.125-132.1980
更新日期:1980-10-01 00:00:00
abstract::Beta interferon (IFN-beta) expression is triggered by double-stranded RNA, a common intermediate in the replication of many viruses including hepatitis C virus (HCV). The recent development of cell culture-grown HCV allowed us to analyze the IFN signaling pathway following virus infection. In this study, we have exami...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01695-07
更新日期:2007-11-01 00:00:00
abstract::UR2 is a newly characterized avian sarcoma virus whose genome contains a unique sequence that is not related to the sequences of other avian sarcoma virus transforming genes thus far identified. This unique sequence, termed ros, is fused to part of the viral gag gene. The product of the fused gag-ros gene of UR2 is a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.228-236.1982
更新日期:1982-04-01 00:00:00
abstract::Thirty-six monoclonal antibodies from mice and three from rats were raised against a peptide corresponding to the immunodominant domain of the transmembrane gp41 protein of human immunodeficiency virus (HIV) type 1 (LGLWGCSGKLIC; amino acid residues 598 to 609). Of these, three monoclonal antibodies from the mice and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.1727-1734.1991
更新日期:1991-04-01 00:00:00
abstract::Friend murine spleen focus-forming virus (SFFV) encodes a glycoprotein designated gp52, which is responsible for the leukemogenic properties of the virus. gp52 lacks a cytoplasmic domain and is defective in its transport to the cell surface. We constructed a chimeric envelope gene which codes for a molecule with an ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4007-4011.1987
更新日期:1987-12-01 00:00:00
abstract:UNLABELLED:The encephalitic response to viral infection requires local chemokine production and the ensuing recruitment of immune and inflammatory leukocytes. Accordingly, chemokine receptors present themselves as plausible therapeutic targets for drugs aimed at limiting encephalitic responses. However, it remains uncl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03421-13
更新日期:2014-09-01 00:00:00