Abstract:
:The blood group P antigen, known to be abundantly expressed on erythroid cells, has been reported to be the cellular receptor for parvovirus B19. We have described the development of recombinant parvovirus B19 vectors with which high-efficiency, erythroid lineage-restricted transduction can be achieved (S. Ponnazhagan, K. A. Weigel, S. P. Raikwar, P. Mukherjee, M. C. Yoder, and A. Srivastava, J. Virol. 72:5224-5230, 1998). However, since a low-level transduction of nonerythroid cells could also be detected and since P antigen is expressed in nonerythroid cells, we reevaluated the role of P antigen in the viral binding and entry into cells. Cell surface expression analyses revealed that approximately 75% of primary human bone marrow mononuclear erythroid cells and approximately 31% of cells in the nonerythroid population were positive for P antigen. Two human erythroleukemia cell lines, HEL and K562, and a human promyelocytic leukemia cell line, HL-60, were also examined for P antigen expression and binding and entry of the vector. HEL and K562 cells showed intermediate levels, whereas HL-60 cells demonstrated high levels of expression of P antigen. However, the efficiency of vector binding to these cells did not correlate with P antigen expression. Moreover, despite P antigen positivity and efficient viral binding, HEL, K562, and HL-60 cells could not be transduced with the vector. Low levels of P antigen expression could also be detected in two primary cell types, human umbilical vein endothelial cells (HUVEC) and normal human lung fibroblasts (NHLF). In addition, vector binding occurred in both cell types and was inhibited by globoside, indicating the involvement of P antigen in virus binding to these cells. These primary cells could be efficiently transduced with the recombinant vector. These data suggest that (i) P antigen is expressed on a variety of cell types and is involved in binding of parvovirus B19 to human cells, (ii) the level of P antigen expression does not correlate with the efficiency of viral binding, (iii) P antigen is necessary but not sufficient for parvovirus B19 entry into cells, and (iv) parvovirus B19 vectors can be used to transduce HUVEC and NHLF. These studies further suggest the existence of a putative cellular coreceptor for efficient entry of parvovirus B19 into human cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Weigel-Kelley KA,Yoder MC,Srivastava Adoi
10.1128/JVI.75.9.4110-4116.2001subject
Has Abstractpub_date
2001-05-01 00:00:00pages
4110-6issue
9eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
杂志文章abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent for KS tumors, multicentric Castleman's disease, and primary effusion lymphomas. Like other herpesvirus capsids, the KSHV capsid is an icosahedral structure composed of six proteins. The capsid shell is made up of the major capsid protein, two tripl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00423-08
更新日期:2008-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) nucleotide sequences encoding p24Gag and the Env C2V3 region were obtained from seven patients who were selected on the basis of having paradoxical seronegativity on a subset of HIV enzyme-linked immunosorbent assay detection kits and having atypical Western blot (immunoblot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5640-5649.1995
更新日期:1995-09-01 00:00:00
abstract::The cancer-related mucin-type carbohydrate neoantigen Tn was found on gp160 and gp120 of human immunodeficiency virus (HIV). Immunoglobulin G (IgG) and IgM monoclonal antibodies (MAbs) against Tn neutralized infection with cell-free virus and blocked fusion between HIV-infected and uninfected cells. This inhibition wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6461-6467.1991
更新日期:1991-12-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) tegument is the least understood component of the virion, and the mechanism of tegument assembly and incorporation into virions during viral egress has not yet been elucidated. In the present study, the addition of tegument proteins (VP13/14, VP16, VP22, and US9) and envelope gl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9934-9951.2002
更新日期:2002-10-01 00:00:00
abstract::We have investigated the effect of Xenopus laevis p53 (Xp53) on transcription from a variety of promoters which are regulated by mouse p53 using a chloramphenicol acetyltransferase reporter system. Although Xp53 transactivated promoters that are up-regulated by mouse p53, it was unable to cause repression. This abilit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7178-7187.1994
更新日期:1994-11-01 00:00:00
abstract::A/duck/Shanghai/28-1/2009(H4N2) (DK28) was isolated from a live poultry market in Shanghai, China. Using PCR and sequencing analysis, we obtained the complete genome sequences of the DK28 virus. The sequence analysis demonstrated that this H4N2 virus was a novel multiple-gene reassortant avian influenza virus (AIV) wh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02179-12
更新日期:2012-11-01 00:00:00
abstract::Previously it was shown that the host-range gene of the Bratislava strain of avain sarcoma virus (B77 virus) spontaneously mutates with a very high rate. The wild-type B77 virus called B77 virus-II, mutates either to virus that efficiently infects duck cells (B77 virus-III) or to virus that does not mutate to the abil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.105-112.1977
更新日期:1977-01-01 00:00:00
abstract::The structural proteins of mature LPP-1 particles and the patterns of protein synthesis after LPP-1 infection have been examined by electrophoresis on sodium dodecyl sulfate polyacrylamide gels. Structural proteins account for 35% of the LPP-1 genome, and proteins that would require about 65% of the total coding capac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.6.841-846.1970
更新日期:1970-12-01 00:00:00
abstract::Two chimpanzees immunized with woodchuck hepatitis virus (WHV) surface antigen (WHsAg) developed antibodies cross-reactive with hepatitis B virus (HBV) surface antigen (HBsAg). After challenge with HBV, one animal was completely protected and the other experienced a subclinical infection, without evidence of liver dis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.895-901.1986
更新日期:1986-12-01 00:00:00
abstract::The recent increase in human cases of influenza A H3N2 variant virus [A(H3N2)v] highlights the need to assess whether seasonal influenza vaccination provides cross-protection against A(H3N2)v virus. Our data demonstrate that the 2011-2012 trivalent inactivated influenza vaccine (TIV) protected ferrets against homologo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02625-12
更新日期:2013-01-01 00:00:00
abstract::Poliovirus-specific RNA-dependent RNA polymerase (3Dpol) was purified to apparent homogeneity. A single polypeptide of an apparent molecular weight of 63,000 catalyzes the synthesis of dimeric and monomeric RNA products in response to the poliovirion RNA template. Analysis of purified 3Dpol by two-dimensional electrop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4563-4568.1989
更新日期:1989-11-01 00:00:00
abstract::Epstein-Barr virus is associated with several human malignancies including Burkitt's lymphoma, nasopharyngeal carcinoma, and Hodgkin's disease (HD). To examine the effect of Epstein-Barr virus nuclear antigen 1 (EBNA-1) in the pathogenesis of HD, we transfected the gene into the HD cell line L428. EBNA-1 expression wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1630-1636.1999
更新日期:1999-02-01 00:00:00
abstract::The oligosaccharide processing and secretion of hepatitis B surface antigen (HBsAg) was studied in Chinese hamster ovary cells stably transfected with the gene coding HBsAg. HBsAg was secreted from cells with a relatively long half time (ca. 5 h). This appeared to be a characteristic of HBsAg itself, since HBsAg-produ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.2.346-353.1984
更新日期:1984-08-01 00:00:00
abstract::The VP6 protein of bluetongue virus possesses a number of activities, including nucleoside triphosphatase, RNA binding, and helicase activity (N. Stauber, J. Martinez-Costas, G. Sutton, K. Monastyrskaya, and P. Roy, J. Virol. 71:7220-7226, 1997). Although the enzymatic functions of the protein have been documented, a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11347-11356.2003
更新日期:2003-11-01 00:00:00
abstract::We determined the sequence of the gene for the virion transactivator protein Vmw65 of herpes simplex virus type 2 (HSV-2), strain 333. An analysis of the coding sequence revealed an overall high degree of primary sequence conservation (86%) relative to the HSV-1 protein, although the carboxy-terminal region which enco...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6705-6713.1991
更新日期:1991-12-01 00:00:00
abstract::The E3 complex transcription unit of adenovirus encodes four major mRNAs (a, c, f, and h) and two minor (d and e) mRNAs with overlapping exons, alternative splice sites, and two polyadenylation sites, termed E3A (upstream) and E3B (downstream). mRNAs a and d use the E3A polyadenylation site, and mRNAs c, e, f, and h u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5914-5923.1992
更新日期:1992-10-01 00:00:00
abstract::Cyclooxygenase-2 (COX-2) is a cellular enzyme in the eicosanoid synthetic pathway that mediates the synthesis of prostaglandins from arachidonic acid. The eicosanoids function as critical regulators of a number of cellular processes, including the acute and chronic inflammatory response, hemostasis, and the innate imm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12529-12536.2004
更新日期:2004-11-01 00:00:00
abstract::Polymorphic basic residues near the C terminus of the prion protein (PrP) in humans and sheep appear to protect against prion disease. In heterozygotes, inhibition of prion formation appears to be dominant negative and has been simulated in cultured cells persistently infected with scrapie prions. The results of nucle...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4351-4360.2000
更新日期:2000-05-01 00:00:00
abstract::Research in the last 2 decades has demonstrated that a specific organelle of the cell nucleus, termed PML nuclear body (PML-NB) or nuclear domain 10 (ND10), is frequently modified during viral infection. This correlates with antagonization of a direct repressive function of individual PML-NB components, such as the PM...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01979-15
更新日期:2016-06-10 00:00:00
abstract::The human T-cell leukemia virus type 1 Tax protein activates the expression of cellular immediate early genes controlled by the serum response element (SRE), which contains both the serum response factor (SRF) binding element (CArG box) and the ternary complex factor (TCF) binding element (Ets box). We show that TCF b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.23.11394-11397.2000
更新日期:2000-12-01 00:00:00
abstract::The epithelium plays a key role in the spread of Lassa virus. Transmission from rodents to humans occurs mainly via inhalation or ingestion of droplets, dust, or food contaminated with rodent urine. Here, we investigated Lassa virus infection in cultured epithelial cells and subsequent release of progeny viruses. We s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02240-09
更新日期:2010-04-01 00:00:00
abstract::The papillomavirus E2 protein is a critical viral regulatory protein with transcription, DNA replication, and genome maintenance functions. We have previously identified the cellular bromodomain protein Brd4 as a major E2-interacting protein and established that it participates in tethering bovine papillomavirus type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00447-07
更新日期:2007-09-01 00:00:00
abstract::Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is a major cause of lower-respiratory-tract disease. hMPV is associated with more severe disease in infants and persons with underlying medical conditions. Animal studies have shown that the hMPV fusion (F) protein alone is capable of inducing pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00106-07
更新日期:2007-08-01 00:00:00
abstract::Polyoma virus particles were purified by equilibrium centrifugation in CsCl. Particles from three regions of the density gradient were examined for infectivity, for their ability to induce expanded pools of deoxyribonucleic acid (DNA) precursors, and for their ability to stimulate the synthesis of DNA. The most infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.1.28-32.1970
更新日期:1970-07-01 00:00:00
abstract:UNLABELLED:Crimean-Congo hemorrhagic fever virus (CCHFV; genus Nairovirus) is an extremely pathogenic member of the Bunyaviridae family. Since handling of the virus requires a biosafety level 4 (BSL-4) facility, little is known about pathomechanisms and host interactions. Here, we describe the establishment of a transc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03691-14
更新日期:2015-06-01 00:00:00
abstract::Escherichia coli K-12 wild type and a uvrA mutant derivative were used to construct isogenic strains bearing one, two, three, or more phage lambda cI genomes and containing increasing concentration of lambda repressor as measured by in vitro operator DNA-binding assays. The survival and phage induction in response to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.3.595-602.1978
更新日期:1978-06-01 00:00:00
abstract::A sequence of polyadenylic acid, homogeneous in composition but heterogeneous in length, was isolated from complete pancreatic RNase digests of both middle and bottom RNAs of cowpea mosaic virus. The polyadenylic acid was 3'-terminal and occurred once per molecule. A fragment consisting of the polyadenylic acid and ap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.243-248.1978
更新日期:1978-05-01 00:00:00
abstract::The proteolytic enzyme, thermolysin, degraded the external segment of the membrane glycoprotein of intact vesicular stomatitis (VS) virions but left behind a small nonglycosylated fragment, presumably embedded in the virion membrane. Other proteases generated membrane-associated glycoprotein fragments differing somewh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.2.237-240.1975
更新日期:1975-08-01 00:00:00
abstract::The family Geminiviridae comprises seven genera differentiated by genome organization, sequence similarity, and insect vector. Capulavirus, an eighth genus, has been proposed to accommodate two newly discovered highly divergent geminiviruses that presently have no known vector. Alfalfa leaf curl virus, identified here...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00453-15
更新日期:2015-09-01 00:00:00
abstract::Herpesviruses are members of a diverse family of viruses that colonize all vertebrates from fish to mammals. Although more than one hundred herpesviruses exist, all are nearly identical architecturally, with a genome consisting of a linear double-stranded DNA molecule (100 to 225 kbp) protected by an icosahedral capsi...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01169-10
更新日期:2010-12-01 00:00:00