Abstract:
:Noninfectious spikeless particles have been obtained from vesicular stomatitis virus (VSV, Indiana serotype) by bromelain or Pronase treatment. They lack the viral glycoprotein (G) but contain all the other viral components (RNA, lipid, and other structural proteins). Triton-solubilized VSV-Indiana glycoprotein preparations, containing the viral G protein as well as lipids (including phospholipids), have been extracted from whole virus preparations, freed from the majority of the detergent, and used to restore infectivity to spikeless VSV. The infectivity of such particles has been found to be enhanced by poly-L-ornithine but inhibited by Trition or homologous antiserum pretreatment. Heat-denatured glycoprotein preparations were not effective in restoring the infectivity to spikeless VSV. Heterologous glycoprotein preparations from the serologically distinct VSV-New Jersey serotype were equally capable of making infectious entities with VSV-Indiana spikeless particles, and the infectivity of these structures was inhibited by VSV-New Jersey antiserum but not by VSV-Indiana antiserum. Purified, detergent-free glycoprotein selectively solubilized from VSV-Indiana by the dialyzable detergent, octylglucoside, also restored infectivity of spikeless virions of VSV-Indiana and VSV-New Jersey.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bishop DH,Repik P,Obijeski JF,Moore NF,Wagner RRdoi
10.1128/JVI.16.1.75-84.1975subject
Has Abstractpub_date
1975-07-01 00:00:00pages
75-84issue
1eissn
0022-538Xissn
1098-5514journal_volume
16pub_type
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doi:10.1128/JVI.69.7.4122-4126.1995
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doi:10.1128/JVI.18.3.1155-1159.1976
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2253-2259.1994
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.78.23.12987-12995.2004
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pub_type: 杂志文章
doi:10.1128/JVI.78.15.7874-7882.2004
更新日期:2004-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02324-16
更新日期:2017-03-13 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5133-5141.1989
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02514-09
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8993-8996.1996
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pub_type: 杂志文章
doi:10.1128/JVI.66.7.4364-4376.1992
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.2.210-216.1969
更新日期:1969-02-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/JVI.70.11.7878-7884.1996
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abstract::Adenovirus has a linear, double-stranded DNA genome that is perceived by the cellular Mre11-Rad50-Nbs1 (MRN) DNA repair complex as a double-strand break. If unabated, MRN elicits a double-strand break repair response that blocks viral DNA replication and ligates the viral genomes into concatemers. There are two sets o...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6967-6972.1996
更新日期:1996-10-01 00:00:00
abstract::The first 86 residues of the Rous sarcoma virus (RSV) Gag protein form a membrane-binding (M) domain that directs Gag to the plasma membrane during budding. Unlike other retroviral Gag proteins, RSV Gag is not myristylated; however, the RSV M domain does contain 11 basic residues that could potentially interact with a...
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pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00