Abstract:
:Cloned DNA from the P155 mutant of polyoma virus transforms cells in culture as efficiently as wild-type DNA, but has a much lower tumorigenic potential when injected into newborn rats. Like cells transformed by wild-type DNA, cells transformed by the mutant DNA grow in low serum concentrations, form colonies in agar suspension, and grow to high saturation densities compared with untransformed cells. They are, however, much less tumorigenic since they transplant 100- to 2,000-fold less efficiently than cells transformed by wild-type DNA. Substitution of the region between 89.7 and 1.8 map units by the corresponding region of P155 DNA decreased the tumorigenicity of wild-type DNA. When this region was isolated from wild-type DNA and substituted in P155 DNA, the tumorigenicity of the latter increased to values comparable to those of wild-type DNA. This showed that the lesion affecting tumorigenicity occurred between 89.7 and 1.8 map units on the polyoma virus genome. Sequence analysis in this region revealed a 12-base-pair deletion between nucleotides 1,347 and 1,360. This identified P155 as an mlt mutant, i.e., a mutant with a deletion from a region which encodes parts of the large and middle T antigens.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gelinas C,Chartrand P,Bastin Mdoi
10.1128/JVI.43.3.1072-1081.1982subject
Has Abstractpub_date
1982-09-01 00:00:00pages
1072-81issue
3eissn
0022-538Xissn
1098-5514journal_volume
43pub_type
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更新日期:2003-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.64.5.2360-2368.1990
更新日期:1990-05-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.43.1.348-351.1982
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pub_type: 杂志文章
doi:10.1128/JVI.46.2.362-370.1983
更新日期:1983-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.151-160.1975
更新日期:1975-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.5.688-693.1969
更新日期:1969-11-01 00:00:00
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doi:10.1128/JVI.35.2.519-530.1980
更新日期:1980-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4338-4345.1990
更新日期:1990-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.3.580-590.1983
更新日期:1983-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.6.2164-2172.1988
更新日期:1988-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.3885-3890.1993
更新日期:1993-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2019-10-29 00:00:00