Abstract:
:Apoptin, a viral death protein derived from chicken anemia virus, displays a number of tumor-specific behaviors. In particular, apoptin is phosphorylated, translocates to the nucleus, and induces apoptosis specifically in tumor or transformed cells, whereas it is nonphosphorylated and remains primarily inactive in the cytoplasm of nontransformed normal cells. Here, we show that in normal cells apoptin can also be activated by the transient transforming signals conferred by ectopically expressed simian virus 40 (SV40) large T antigen (LT), which rapidly induces apoptin's phosphorylation, nuclear accumulation, and the ability to induce apoptosis. Further analyses with mutants of LT showed that the minimum domain capable of inducing all three of apoptin's tumor-specific properties resided in the N-terminal J domain, a sequence which is largely shared by SV40 small t antigen (st). Interestingly, the J domain in st, which lacks its own nuclear localization signal (NLS), required nuclear localization to activate apoptin. These results reveal the existence of a cellular pathway shared by conditions of transient transformation and the stable cancerous or precancerous state, and they support a model whereby a transient transforming signal confers on apoptin both the upstream activity of phosphorylation and the downstream activity of nuclear accumulation and apoptosis induction. Such a pathway may reflect a general lesion contributing to human cancers.
journal_name
J Viroljournal_title
Journal of virologyauthors
Zhang YH,Kooistra K,Pietersen A,Rohn JL,Noteborn MHdoi
10.1128/JVI.78.18.9965-9976.2004subject
Has Abstractpub_date
2004-09-01 00:00:00pages
9965-76issue
18eissn
0022-538Xissn
1098-5514pii
78/18/9965journal_volume
78pub_type
杂志文章abstract::Many viruses express inhibitors of programmed cell death (apoptosis), thereby countering host defenses that would otherwise rapidly clear infected cells. To counter this, viruses such as adenoviruses and herpesviruses express recognizable homologs of the mammalian prosurvival protein Bcl-2. In contrast, the majority o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01115-12
更新日期:2012-11-01 00:00:00
abstract::Ocular herpes simplex keratitis (HSK) is a consequence of viral reactivations from trigeminal ganglia (TG) and occurs almost exclusively in the same eye in humans. In our murine oro-ocular (OO) model, herpes simplex virus 1 (HSV-1) inoculation in one side of the lip propagates virus to infect the ipsilateral TG. Repli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01586-19
更新日期:2019-11-26 00:00:00
abstract::The temporal sequence of coronavirus plus-strand and minus-strand RNA synthesis was determined in 17CL1 cells infected with the A59 strain of mouse hepatitis virus (MHV). MHV-induced fusion was prevented by keeping the pH of the medium below pH 6.8. This had no effect on the MHV replication cycle, but gave 5- to 10-fo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.328-334.1986
更新日期:1986-01-01 00:00:00
abstract::The yeast two-hybrid system was used to catalog all detectable interactions among the P2 nonstructural cleavage products of poliovirus type 1 (Mahoney). Evidence has been obtained for specific associations among 2A(pro), 2BC, 2C, and 2B. Specifically, 2A(pro) can interact with itself and 2BC and its cleavage products ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1297-1307.1998
更新日期:1998-02-01 00:00:00
abstract::Heterozygous replicative form molecules of bacteriophage phiX174 deoxyribonucleic acid (DNA) have been constructed in vitro. These are composed of viral strands extracted from purified preparations of phage bearing ts mutations and complementary strands of either half length or full length synthesized with purified DN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.5.603-611.1971
更新日期:1971-05-01 00:00:00
abstract::We have purified the seven virus-specific RNAs which were previously shown to be induced in Sac(-) cells upon infection with mouse hepatitis virus strain A59 (W. J. M. Spaan, P. J. M. Rottier, M. C. Horzinek, and B. A. M. van der Zeijst, Virology 108:424-434, 1981). The individual RNAs, prepared by agarose gel electro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.20-26.1981
更新日期:1981-04-01 00:00:00
abstract::Efforts to induce broadly reactive immunity against human immunodeficiency virus type 1 (HIV-1) have been impaired by the extent of sequence variation exhibited by this lentivirus. Cytotoxic T lymphocytes (CTL) specific for other viruses such as influenza virus have been shown to mediate immunity against divergent vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3145-3153.1994
更新日期:1994-05-01 00:00:00
abstract::Transfection has been found and characterized in pneumococcus. For replicating omega3 phage DNA extracted from infected cells, transfection was relatively efficient and rose linearly with DNA concentration and quadratically with time, according to T(T - 3.5) min(2). For mature DNA extracted from phage particles, trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.60-72.1978
更新日期:1978-01-01 00:00:00
abstract::The coding capacity of human cytomegalovirus (HCMV) for glycoproteins by far exceeds that of other herpesviruses. Few of these proteins have been characterized so far. We have investigated the gene product of reading frame UL132. The putative protein product of UL132 is a glycoprotein with a theoretical mass of 29.8 k...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11837-11847.2005
更新日期:2005-09-01 00:00:00
abstract::Sonchus yellow net virus is a plant nucleorhabdovirus whose nucleocapsid (N), phosphoprotein (P), and polymerase (L) proteins form large viroplasms in the nuclei of infected plants (C. R. F. Martins, J. A. Johnson, D. M. Lawrence, T. J. Choi, A. Pisi, S. L. Tobin, D. Lapidus, J. D. O. Wagner, S. Ruzin, K. McDonald, an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02349-06
更新日期:2007-05-01 00:00:00
abstract::The E4orf6 protein of group C adenovirus is an oncoprotein that, in association with the E1B 55-kDa protein and by E1B-independent means, promotes virus replication. An arginine-faced amphipathic alpha-helix in the E4orf6 protein is required for the E4orf6 protein to direct nuclear localization of the E1B 55-kDa prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1475-1487.2002
更新日期:2002-02-01 00:00:00
abstract::The human cytomegalovirus (HCMV) UL138 protein downregulates the cell surface expression of the multidrug resistance-associated protein 1 (MRP1) transporter. We examined the genetic requirements within UL138 for MRP1 downregulation. We determined that the acidic cluster dileucine motif is essential for UL138-mediated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00430-19
更新日期:2019-05-15 00:00:00
abstract::Nipah virus (NiV) and Hendra virus (HeV) are emerging zoonotic viruses and the causative agents of severe respiratory disease and encephalitis in humans. Little is known about the mechanisms that govern the development of respiratory and neurological disease. Using a hamster model of lethal NiV and HeV infection, we d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00473-11
更新日期:2011-08-01 00:00:00
abstract::This report describes the results of experiments to determine whether chimeras between a retrovirus and portions of Ty3 are active in vivo. A chimera between Ty3 and a Neo(r)-marked Moloney murine leukemia virus (M-MuLV) was constructed. The C-terminal domain of M-MuLV integrase (IN) was replaced with the C-terminal d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4297-4307.1998
更新日期:1998-05-01 00:00:00
abstract::Clones of virogenic simian virus 40 (SV40)-transformed hamster kidney cells were exposed to medium deficient in the essential amino acids leucine, arginine, or methionine. Infectious virus was induced after deprivation periods of from 24 to 32 hr. The highest yields of infectious SV40 were obtained from cultures depri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.3.448-453.1972
更新日期:1972-03-01 00:00:00
abstract::For human parainfluenza virus type 3 and many other paramyxoviruses, membrane fusion mediated by the fusion protein (F) has a stringent requirement for the presence of the homotypic hemagglutinin-neuraminidase protein (HN). With the goal of gaining further insight into the role of HN in the fusion process, we develope...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.6.3647-3654.2003
更新日期:2003-03-01 00:00:00
abstract::Superinfection of Corynebacterium diphtheriae C7(beta) by heteroimmune phage gamma is productive, whereas superinfection by gamma-bin mutants is for the most part nonproductive. Exclusion of gamma-bin phage occurred after its DNA had penetrated and was partially expressed in the heteroimmune lysogen. All of the infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.526-532.1980
更新日期:1980-11-01 00:00:00
abstract::Poliovirus proteinase 2A (2Apro) is autocatalytically released from the viral polyprotein by cleavage in cis of a Tyr-Gly dipeptide at its own amino terminus, resulting in separation of the P1 structural and P2-P3 nonstructural protein precursors. A second Ty-Gly dipeptide within 3D polymerase is cleaved by 2Apro in t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3330-3338.1992
更新日期:1992-06-01 00:00:00
abstract::The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the type species of alphabaculoviruses, is an enveloped DNA virus that infects lepidopteran insects and is commonly known as a vector for protein expression and cell transduction. AcMNPV belongs to a diverse group of viral and bacterial pat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00111-18
更新日期:2018-05-14 00:00:00
abstract::Monoclonal antibodies (MAbs) directed against the E2 glycoprotein of mouse hepatitis virus (MHV) have been classified according to their ability to bind to either of the two purified 90,000-molecular-weight subunits (90K subunits) of the 180K peplomeric glycoprotein E2. Correlation with previously reported information...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3051-3055.1990
更新日期:1990-06-01 00:00:00
abstract:UNLABELLED:Marburg virus (MARV) infection is a lethal hemorrhagic fever for which no licensed vaccines or therapeutics are available. Development of appropriate medical countermeasures requires a thorough understanding of the interaction between the host and the pathogen and the resulting disease course. In this study,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01147-15
更新日期:2015-10-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection requires cell surface expression of CD4. Costimulation of CD8(+)/CD4(-) T lymphocytes by anti-CD3 and anti-CD28 antibodies or by allogeneic dendritic cells induced expression of CD4 and rendered these CD8 cells susceptible to HIV-1 infection. Naive CD45RA+ cells re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9054-9060.1998
更新日期:1998-11-01 00:00:00
abstract:UNLABELLED:Hepatitis B virus (HBV) infects hundreds of millions of people worldwide and causes acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HBV is an enveloped virus with a relaxed circular (RC) DNA genome. In the nuclei of infected human hepatocytes, conversion of RC DNA from the incoming viri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01263-15
更新日期:2015-10-21 00:00:00
abstract::Group A rotaviruses are major pathogens causing acute gastroenteritis in children and animals. To determine if group A rotavirus replicates and induces disease in rats, antibody-negative Lewis neonatal or adult rats were inoculated orally with tissue culture-adapted human (Wa, WI61, and HAL1166), simian (rhesus rotavi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.1.41-57.2002
更新日期:2002-01-01 00:00:00
abstract::The gH/gL complex plays an essential role in virus entry and cell-cell spread of herpes simplex virus (HSV). Very few immunologic reagents were previously available to either identify important functional regions or gain information about structural features of this complex. Therefore, we generated and characterized a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.6.2596-2608.2006
更新日期:2006-03-01 00:00:00
abstract:UNLABELLED:Human metapneumovirus (HMPV), a recently discovered paramyxovirus, infects nearly 100% of the world population and causes severe respiratory disease in infants, the elderly, and immunocompromised patients. We previously showed that HMPV binds heparan sulfate proteoglycans (HSPGs) and that HMPV binding requir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01362-16
更新日期:2016-09-29 00:00:00
abstract::A continuous cell line of highly contact-inhibited cells (NIH/3T3) has been developed from NIH Swiss mouse embryo cultures. Its growth properties are similar to those of 3T3 and BALB/3T3. Although 3T3 is relatively insensitive to focus formation by murine sarcoma viruses, cloned lines of both NIH/3T3 and BALB/3T3 have...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.5.549-553.1969
更新日期:1969-11-01 00:00:00
abstract::Epstein-Barr virus (EBV) SM protein is an essential nuclear shuttling protein expressed by EBV early during the lytic phase of replication. SM acts to increase EBV lytic gene expression by binding EBV mRNAs and enhancing accumulation of the majority of EBV lytic cycle mRNAs. SM increases target mRNA stability and nucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01483-09
更新日期:2009-11-01 00:00:00
abstract::The genome structure of a newly isolated sarcoma virus, Y73, was studied. Y73 is a defective, potent sarcomagenic virus and contains 4.8-kilobase (kb) RNA as its genome; in contrast, helper virus associated with Y73 had 8.5-kb RNA, similar to other avian leukemia viruses. Fingerprinting analysis these RNAs demonstrate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.2.430-437.1981
更新日期:1981-05-01 00:00:00
abstract::Linear hepadnavirus DNA in primary hepatocyte cultures efficiently participates in intra- and intermolecular nonhomologous recombination at its ends. The products of this recombination are (i) monomeric covalently closed circular DNAs (cccDNAs) with deletions and insertions around the site of joining and (ii) oligomer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4029-4036.1995
更新日期:1995-07-01 00:00:00