Abstract:
:Heterozygous replicative form molecules of bacteriophage phiX174 deoxyribonucleic acid (DNA) have been constructed in vitro. These are composed of viral strands extracted from purified preparations of phage bearing ts mutations and complementary strands of either half length or full length synthesized with purified DNA polymerase, in vitro, on DNA from am3 phage. In infections with such heterozygous DNA, involving mutations in each of four different cistrons, phage with the genotype of the complementary strand comprised 1 to 20% of the total phage produced by a spheroplast population. From single-burst analysis of the progeny from DNA heterozygous in one cistron (B), it appears that those phage with the genotype of the complementary strand arise as major components in a small proportion of the infected cells rather than comprising a minor component in most cells. The implications of such a pattern of expression are discussed with respect to mechanisms of phage DNA synthesis.
journal_name
J Viroljournal_title
Journal of virologyauthors
Merriam V,Dumas LB,Sinsheimer RLdoi
10.1128/JVI.7.5.603-611.1971subject
Has Abstractpub_date
1971-05-01 00:00:00pages
603-11issue
5eissn
0022-538Xissn
1098-5514journal_volume
7pub_type
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