Abstract:
:MicroRNAs (miRNAs) have emerged as key regulators in many biological processes, from development to defense, at almost all organismal levels. Recently, their role has been highlighted in pathogen-host interactions. Emerging evidence from a variety of virus-host systems indicates that cellular as well as virally encoded miRNAs influence viral replication. Here, we report changes in expression levels of host miRNAs upon ascovirus infection in an insect cell line and investigated the role of a host miRNA, Hz-miR24, in the host-virus system. We found that Hz-miR24 is differentially expressed following virus infection, with an increase in its expression levels late in infection. Experimental evidence demonstrated that Hz-miR24 downregulates ascoviral DNA-dependent RNA polymerase and its beta subunit transcript levels late in infection. The specific miRNA-target interactions were investigated and confirmed using the ectopic expression of Hz-miR24 and a green fluorescent protein-based reporter system. Further, the expression of the target gene was substantially enhanced in cells transfected with a synthesized inhibitor of Hz-miR24. These findings suggest that ascoviruses manipulate host miRNAs that in turn regulate the expression of their genes at specific time points after infection. To our knowledge, this is the first cellular miRNA reported to interact with an insect virus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hussain M,Asgari Sdoi
10.1128/JVI.01794-09subject
Has Abstractpub_date
2010-01-01 00:00:00pages
612-20issue
1eissn
0022-538Xissn
1098-5514pii
JVI.01794-09journal_volume
84pub_type
杂志文章abstract::We reported previously that an N-terminally truncated insulinlike growth factor I receptor (IGFR) fused to avian sarcoma virus UR2 gag p19 had a greater transforming potential than did the native IGFR, but it failed to cause tumors in vivo. To investigate whether the 36 amino acids (aa) of the IGFR extracellular (EC) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.9-18.1993
更新日期:1993-01-01 00:00:00
abstract::A soluble system was developed that could support DNA replication in simian virus 40 (SV40) chromosomes. DNA synthesis in this system required the presence of purified SV40 large tumor antigen, SV40 chromosomes prepared from virus-infected monkey cells, a crude extract from HeLa cells, and several low-molecular-weight...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4820-4829.1990
更新日期:1990-10-01 00:00:00
abstract::We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05809-11
更新日期:2012-04-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) was isolated from the total peripheral blood mononuclear cell population and the monocyte-macrophage adherent cell population of three seropositive green monkeys originating from Kenya. SIV from these African green monkeys (SIVagm) was isolated and continuously produced with the MOL...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.11.4123-4128.1988
更新日期:1988-11-01 00:00:00
abstract::Bacteriophage T4 gene 32 encodes a DNA unwinding protein required for DNA replication, repair, and recombination. Gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.490-494.1976
更新日期:1976-08-01 00:00:00
abstract::The helicase-like 1a and polymerase-like 2a proteins of brome mosaic virus (BMV) are required for viral RNA replication in vivo, are present in membrane-bound viral RNA polymerase extracts, and share conservation with the many other members of the alphavirus-like superfamily. To better understand BMV RNA replication a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8908-8916.1996
更新日期:1996-12-01 00:00:00
abstract:UNLABELLED:Mumps virus (MuV), a paramyxovirus containing a negative-sense nonsegmented RNA genome, is a human pathogen that causes an acute infection with symptoms ranging from parotitis to mild meningitis and severe encephalitis. Vaccination against mumps virus has been effective in reducing mumps cases. However, rece...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03673-13
更新日期:2014-04-01 00:00:00
abstract::Sindbis virus nonstructural polyproteins and their cleavage products are believed to be essential components of viral RNA replication and transcription complexes. Although numerous studies have investigated the effect of mutations in nsP1-, nsP2-, and nsP4-coding regions on Sindbis virus-specific RNA synthesis, relati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5781-5791.1994
更新日期:1994-09-01 00:00:00
abstract::Dactylosporangiophage A1 has a polygonal head (75 nm) with spherical capsomeres (3 nm) and a noncontractile tail (200 by 10 nm) with cross-striations which is terminated with at least three prongs which are used for attachment. It contains double-stranded deoxyribonucleic acid and produces very little lysis. Intracell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.2.210-216.1969
更新日期:1969-02-01 00:00:00
abstract::The effect of human cytomegalovirus infection on cellular DNA synthesis in human fibroblasts was measured by fluorometry and by incorporation of radiolabeled thymidine. The results show that although HCMV infection stimulates cellular DNA synthesis in both quiescent and serum-stimulated cells, radiolabeled thymidine i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6402-6404.1996
更新日期:1996-09-01 00:00:00
abstract::In an attempt to identify the molecules involved in the pathogenesis of prion diseases, we performed cDNA subtraction on the brain tissues of mice affected with an experimental prion disease and the unaffected control. The genes identified as being upregulated in the prion-affected brain tissue included those encoding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.1.411-417.2000
更新日期:2000-01-01 00:00:00
abstract::The potential use of the modified vaccinia virus Ankara (MVA) strain as a live recombinant vector to deliver antigens and elicit protective immune responses against infectious diseases demands a comprehensive understanding of the effect of MVA infection on human host gene expression. We used microarrays containing mor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.11.5820-5834.2004
更新日期:2004-06-01 00:00:00
abstract::Affinity-purified medium T antigen encoded by NG59, a nontransforming mutant of polyomavirus, is specifically associated with a protein of 72,000 daltons (72K protein). Medium T antigens of wild-type polyomavirus and the transformation-competent mutant dl8 are not associated with the 72K protein. Instead, they form a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.405-410.1987
更新日期:1987-02-01 00:00:00
abstract::We tested the ability of human monoclonal antibodies (immunoglobulin G1b12 [IgG1b12] and 19b) and CD4-based molecules (CD4-IgG2 and soluble CD4 [sCD4]) to neutralize human immunodeficiency virus type 1 directly from the plasma of seropositive donors in an ex vivo neutralization assay. IgG1b12 and CD4-IgG2, at concentr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2586-2592.1996
更新日期:1996-04-01 00:00:00
abstract::In 2010 and 2011, several devastating Newcastle disease (ND) outbreaks occurred in China, affecting broilers, layers, and breeders. The CK-JSX1-201005 virus was isolated from broiler breeder flocks vaccinated with the classical ND virus (NDV) vaccine program, but laying rate decreased from 80% to 30 to 40% in the clin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02314-12
更新日期:2012-11-01 00:00:00
abstract::It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered reg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01781-15
更新日期:2015-11-01 00:00:00
abstract::Activated Notch1 (AcN1) alleles cooperate with oncogenes from DNA tumor viruses in transformation of epithelial cells. AcN1 signaling has pleiotropic effects, and suggested oncogenic roles include driving proliferation through cyclin D1 or the generation of resistance to apoptosis on matrix withdrawal through a phosph...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.12.7106-7112.2003
更新日期:2003-06-01 00:00:00
abstract::To study the variability of human immunodeficiency virus type 1 (HIV-1), we used immunotoxins to select for variants within a population of H9 cells persistently infected with a molecular clone of HIV-1 designated NL4-3. Chimeric immunotoxin CD4-PE40 (a chimeric fusion protein consisting of the amino-terminal two doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.75-81.1995
更新日期:1995-01-01 00:00:00
abstract::Polymorphic basic residues near the C terminus of the prion protein (PrP) in humans and sheep appear to protect against prion disease. In heterozygotes, inhibition of prion formation appears to be dominant negative and has been simulated in cultured cells persistently infected with scrapie prions. The results of nucle...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4351-4360.2000
更新日期:2000-05-01 00:00:00
abstract::The purpose of this study was to determine whether a cell surface protein that can serve as coreceptor for herpes simplex virus type 1 (HSV-1) entry, herpesvirus entry mediator (previously designated HVEM but renamed HveA), also mediates HSV-1-induced cell-cell fusion. We found that transfection of DNA from KOS-804, a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5802-5810.1998
更新日期:1998-07-01 00:00:00
abstract::Vaccine-induced memory T cells localized at mucosal sites can provide rapid protection from viral infection. All-trans-retinoic acid (ATRA) has been shown to act physiologically to induce the expression of gut-homing receptors on lymphocytes. We tested whether the administration of exogenous ATRA during a systemic vac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00781-11
更新日期:2011-08-01 00:00:00
abstract::A recombinant Epstein-Barr virus (EBV) was constructed, with a positive-selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte gro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.5068-5074.1993
更新日期:1993-08-01 00:00:00
abstract::The herpesvirus saimiri (HVS) immediate-early gene product encoded by open reading frame (ORF) 57 shares limited amino acid homology with HSV-1 ICP27 and Epstein-Barr virus BMLF1, both regulatory proteins. The ORF 57 gene has been proposed to be spliced based on the genome sequence, and here we confirm the intron-exon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.857-861.1998
更新日期:1998-01-01 00:00:00
abstract::Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1108-1114.1974
更新日期:1974-11-01 00:00:00
abstract::Nonhuman primates are widely used to study correlates of protective immunity in AIDS research. Successful cellular immune responses have been difficult to identify because heterogeneity within macaque major histocompatibility complex (MHC) genes results in quantitative and qualitative differences in immune responses. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01841-06
更新日期:2007-01-01 00:00:00
abstract::Six nonoverlapping peptides of the neuraminidase (NA) glycoprotein of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8 virus) were found to be immunogenic for proliferating T cells when injected into BALB/c mice in Freund adjuvant. T cells elicited by peptide immunization could recognize PR8 virus in vitro. However, onl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.672-676.1991
更新日期:1991-02-01 00:00:00
abstract::A phylogenetic analysis of 52 published and 37 new nucleoprotein (NP) gene sequences addressed the evolution and origin of human and swine influenza A viruses. H1N1 human and classical swine viruses (i.e., those related to Swine/Iowa/15/30) share a single common ancestor, which was estimated to have occurred in 1912 t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3704-3714.1991
更新日期:1991-07-01 00:00:00
abstract::Recent evidence suggests that the type II feline coronavirus (FCoV) strains 79-1146 and 79-1683 have arisen from a homologous RNA recombination event between FCoV type I and canine coronavirus (CCV). In both cases, the template switch apparently took place between the S and M genes, giving rise to recombinant viruses ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4508-4514.1998
更新日期:1998-05-01 00:00:00
abstract::Polyomavirus small t antigen was purified from genetically engineered Escherichia coli and used as the immunogen for the production of polyclonal and monoclonal antibodies. A new series of plasmids for increased expression of polyomavirus T antigens or a T antigen-beta-galactosidase fusion protein was constructed by r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.1075-1084.1986
更新日期:1986-12-01 00:00:00
abstract::Early sensing of viral components or infection-induced tissue damage is a prerequisite for the successful control of pathogenic viruses by the host innate immune system. Recent results from our laboratory show how immune cells use the DNA-sensing machinery to detect intracellular damage generated early during infectio...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01081-16
更新日期:2017-06-26 00:00:00