Abstract:
UNLABELLED:Marburg virus (MARV) infection is a lethal hemorrhagic fever for which no licensed vaccines or therapeutics are available. Development of appropriate medical countermeasures requires a thorough understanding of the interaction between the host and the pathogen and the resulting disease course. In this study, 15 rhesus macaques were sequentially sacrificed following aerosol exposure to the MARV variant Angola, with longitudinal changes in physiology, immunology, and histopathology used to assess disease progression. Immunohistochemical evidence of infection and resulting histopathological changes were identified as early as day 3 postexposure (p.e.). The appearance of fever in infected animals coincided with the detection of serum viremia and plasma viral genomes on day 4 p.e. High (>10(7) PFU/ml) viral loads were detected in all major organs (lung, liver, spleen, kidney, brain, etc.) beginning day 6 p.e. Clinical pathology findings included coagulopathy, leukocytosis, and profound liver destruction as indicated by elevated liver transaminases, azotemia, and hypoalbuminemia. Altered cytokine expression in response to infection included early increases in Th2 cytokines such as interleukin 10 (IL-10) and IL-5 and late-stage increases in Th1 cytokines such as IL-2, IL-15, and granulocyte-macrophage colony-stimulating factor (GM-CSF). This study provides a longitudinal examination of clinical disease of aerosol MARV Angola infection in the rhesus macaque model. IMPORTANCE:In this study, we carefully analyzed the timeline of Marburg virus infection in nonhuman primates in order to provide a well-characterized model of disease progression following aerosol exposure.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lin KL,Twenhafel NA,Connor JH,Cashman KA,Shamblin JD,Donnelly GC,Esham HL,Wlazlowski CB,Johnson JC,Honko AN,Botto MA,Yen J,Hensley LE,Goff AJdoi
10.1128/JVI.01147-15subject
Has Abstractpub_date
2015-10-01 00:00:00pages
9875-85issue
19eissn
0022-538Xissn
1098-5514pii
JVI.01147-15journal_volume
89pub_type
杂志文章abstract::Poxviruses express proteins that limit host immune responses to infection. For example, the molluscum contagiosum virus MC160 protein inhibits tumor necrosis factor alpha (TNF-alpha)-induced NF-kappaB activation. This event correlates with MC160-induced IKK1 protein degradation, suggesting a mechanism for the above-me...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02009-08
更新日期:2009-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00318-18
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abstract::The human papillomavirus 1 (HPV-1) virion is composed of two virally encoded proteins: a 57,000-molecular-weight polypeptide (57K polypeptide), which is the product of the L1 open reading frame (ORF), and a 78K polypeptide, which is derived from the L2 ORF. The 57K (L1) product, which represents the major structural c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2793-2799.1987
更新日期:1987-09-01 00:00:00
abstract::Termination of mRNA synthesis in vesicular stomatitis virus (VSV), the prototypic rhabdovirus, is controlled by a 13-nucleotide gene end sequence which comprises the conserved tetranucleotide 3'-AUAC-5', the U(7) tract and the intergenic dinucleotide. mRNAs terminated at this sequence possess 100- to 300-nucleotide-lo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.6901-6913.2001
更新日期:2001-08-01 00:00:00
abstract::The mechanism of cellular src (c-src) transduction by a transformation-defective deletion mutant, td109, of Rous sarcoma virus was studied by sequence analysis of the recombinational junctions in three td109-derived recovered sarcoma viruses (rASVs). Our results show that two rASVs have been generated by recombination...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.3.556-563.1986
更新日期:1986-09-01 00:00:00
abstract::Single-envelope human immunodeficiency virus (HIV) vaccines have been studied for more than a decade, with some successes in homologous challenge experiments in nonhuman primates but with no clear successes in clinical trials. To gain insight into the breadth of the immunity elicited by such vaccines, we have dissecte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4231-4236.2003
更新日期:2003-04-01 00:00:00
abstract::Forty isolates of herpes simplex virus were compared by means of cross-neutralization curves. The 11 oral isolates were serotype 1, and all 29 genital/anal isolates were serotype 2. The cytopathic effects of the two serotypes were consistently different. Passage of strains of type 1 and type 2 in mice and in rabbits y...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.1.51-59.1970
更新日期:1970-01-01 00:00:00
abstract::Analysis of six monoclonal antibody-resistant (mar) mutants in herpes simplex virus type 1 glycoprotein B identified two type-common (II and III) and two type-specific (I and IV) antigenic sites on this molecule. To derive additional information on the location of these sites, mar mutations were mapped and nucleotide ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.730-738.1989
更新日期:1989-02-01 00:00:00
abstract::The recent finding that the E1 glycoproteins of murine coronaviruses contain only O-linked oligosaccharides suggested that this unusual modification might be a distinguishing feature of coronaviruses and might play an essential role in the life cycle of this family of viruses. To examine these possibilities, we analyz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.804-812.1982
更新日期:1982-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6394-6400.2000
更新日期:2000-07-01 00:00:00
abstract::Similar to other positive-sense, single-stranded RNA viruses, hepatitis C virus (HCV) replicates its genome in a remodeled intracellular membranous structure known as the membranous web (MW). To date, the process of MW formation remains unclear. It is generally acknowledged that HCV nonstructural protein 4B (NS4B) can...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01785-12
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abstract::Upon infection of Japanese encephalitis virus (JEV), baby hamster kidney (BHK-21) and Chinese hamster ovary (CHO) cells were killed by a mechanism involved in apoptosis. While readily established in a variety of cell lines, JEV persistence has never been successfully instituted in BHK-21 and CHO cells. Since stable ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9844-9854.1998
更新日期:1998-12-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef is heavily targeted by CD8(+) T lymphocytes (CTLs) during acute infection and therefore is included in many candidate vaccines. We investigated whether CTL targeting of Nef during acute infection contributes to immune control by disrupting the funct...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00482-14
更新日期:2014-07-01 00:00:00
abstract::A novel species of 30S RNA has been detected in a variety of mouse cell lines. The 30S RNA is specifically packaged by helper-independent type C viruses propagated in such cells. Nucleic acid hybridization detects no homology between the 30SRNA and the genomic RNA of helper-independent mouse type C viruses. The proper...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.115-123.1978
更新日期:1978-01-01 00:00:00
abstract::Due to various advantageous features there is current interest in retroviral vectors derived from primate foamy viruses (PFVs). Two PFV cis-acting sequences have been mapped in the 5' region of the RNA (pre-)genome and in the 3' pol genomic region. In order to genetically separate PFV packaging constructs from vector ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.3141-3148.2000
更新日期:2000-04-01 00:00:00
abstract::Four mutants of the virulent Mahoney strain of poliovirus were generated by introducing mutations in nucleotides (nt) 128 to 134 of the genome, a region that contains a part of the stem-loop II (SLII) structure located within the internal ribosomal entry site (IRES; nt 120 to 590) (K. Shiroki, T. Ishii, T. Aoki, Y. Ot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2398-2405.1998
更新日期:1998-03-01 00:00:00
abstract::Canine distemper virus attachment (hemagglutinin [H] equivalent) and fusion (F) antigens were purified by affinity chromatography with monoclonal antibodies. The purified antigens were used to immunize groups of three dogs. Radioimmune precipitation assays with sera from these animals showed that the F antigen prepara...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.536-541.1986
更新日期:1986-05-01 00:00:00
abstract::It was demonstrated that Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) were nonessential for B-lymphocyte growth transformation. We revisited this issue by producing a large quantity of EBER-deleted EBV by using an Akata cell system. Although the EBER-deleted virus efficiently infected B lymphocytes, its 50% tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4298-4307.2005
更新日期:2005-04-01 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68) infection of mice provides a tractable small-animal system for assessing viral requirements for establishment of and reactivation from latency. The M2 gene product has no homology to any known proteins but has been shown to play a role in both the establishment of MHV68 latency and r...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4731-4738.2003
更新日期:2003-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1909-1917.1999
更新日期:1999-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00109-15
更新日期:2015-05-01 00:00:00
abstract::Among the five serine incorporator (SERINC) family members, SERINC5 (Ser5) was reported to strongly inhibit HIV-1 replication, which is counteracted by Nef. Ser5 produces 5 alternatively spliced isoforms: Ser5-001 has 10 putative transmembrane domains, whereas Ser5-004, -005, -008a, and -008b do not have the last one....
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2017-04-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8279-8288.1997
更新日期:1997-11-01 00:00:00
abstract::Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations requi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.24.13819-13828.2004
更新日期:2004-12-01 00:00:00
abstract::The γ134.5 gene of herpes simplex virus 1 (HSV-1) encodes a virulence factor that promotes viral pathogenesis. Although it perturbs TANK-binding kinase 1 (TBK1) in the complex network of innate immune pathways, the underlying mechanism is obscure. Here we report that HSV-1 γ134.5 targets stimulator of interferon genes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01015-18
更新日期:2018-09-26 00:00:00
abstract:UNLABELLED:Despite encoding multiple viral proteins that modulate the retinoblastoma (Rb) protein in a manner classically defined as inactivation, human cytomegalovirus (HCMV) requires the presence of the Rb protein to replicate efficiently. In uninfected cells, Rb controls numerous pathways that the virus also command...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01718-15
更新日期:2015-12-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a ubiquitous human pathogen associated with Burkitt's lymphoma and nasopharyngeal carcinoma. Although the EBV genome harbors more than a hundred genes, a full transcription map with EBV polyadenylation profiles remains unknown. To elucidate the 3' ends of all EBV transcripts genome-wide, we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01593-18
更新日期:2019-01-04 00:00:00
abstract::Gene therapy is proposed as a novel therapeutic strategy for treating glioblastoma multiforme (GBM), a devastating brain cancer. In the clinic, antivector immune responses pose formidable challenges. Herein we demonstrate that high-capacity adenovirus vectors (HC-Ads) carrying the conditional cytotoxic gene herpes sim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00232-08
更新日期:2008-05-01 00:00:00
abstract::After isolation, the DNA of simian virus 40 appeared as a negative supertwist (form I) or as an open circle with at least one single-strand scission (form II). Under the denaturation conditions usually applied, such as heating in the presence of formaldehyde or application of alkali, form I molecules could appear as "...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.585-598.1975
更新日期:1975-03-01 00:00:00