Abstract:
:Analysis of six monoclonal antibody-resistant (mar) mutants in herpes simplex virus type 1 glycoprotein B identified two type-common (II and III) and two type-specific (I and IV) antigenic sites on this molecule. To derive additional information on the location of these sites, mar mutations were mapped and nucleotide alterations were identified by DNA sequencing. Each mutant carried a single amino acid substitution resulting from a G-to-A base transition. Alterations affecting antibody neutralization were identified at residues 473, 594, 305, and 85 for mutants in sites I through IV, respectively. Two clonally distinct site II antibodies each selected mar mutants (Gly to Arg at residue 594) that exhibited a reduction in the rate of entry (roe) into host cells. A site II mar revertant that regained sensitivity to neutralization by site II antibodies also showed normal entry kinetics. DNA sequencing of this virus identified a single base reversion of the site II mar mutation, resulting in restoration of the wild-type sequence (Arg to Gly). This finding demonstrated that the mar and roe phenotypes were the result of a single mutation. To further define structures that contributed to antibody recognition, monoclonal antibodies specific for all four sites were tested for their ability to immune precipitate a panel of linker-insertion mutant glycoprotein B molecules. Individual polypeptides that contained single insertions of 2 to 28 amino acids throughout the external domain were not recognized or were recognized poorly by antibodies specific for sites II and III, whereas no insertion affected antibody recognition of sites I and IV. mar mutations affecting either site II or III were previously shown to cause temperature-sensitive defects in glycoprotein B glycosylation, and variants altered in both these sites were temperature sensitive for virus production. Taken together, the data indicate that antigenic sites II and III are composed of higher-order structures whose integrity is linked with the ability of glycoprotein B to function in virus infectivity.
journal_name
J Viroljournal_title
Journal of virologyauthors
Highlander SL,Dorney DJ,Gage PJ,Holland TC,Cai W,Person S,Levine M,Glorioso JCdoi
10.1128/JVI.63.2.730-738.1989subject
Has Abstractpub_date
1989-02-01 00:00:00pages
730-8issue
2eissn
0022-538Xissn
1098-5514journal_volume
63pub_type
杂志文章abstract::Bovine herpesvirus 1 (BHV-1) infected cell protein 0 (bICP0) stimulates productive infection, in part by activating viral gene expression. The C(3)HC(4) zinc RING finger of bICP0 is crucial for activating viral transcription and productive infection. In this study, we used a bacterial artificial chromosome containing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01348-08
更新日期:2008-12-01 00:00:00
abstract:UNLABELLED:Phenotypic diversity in prion diseases can be specified by prion strains in which biological traits are propagated through an epigenetic mechanism mediated by distinct PrP(Sc) conformations. We investigated the role of host-dependent factors on phenotypic diversity of chronic wasting disease (CWD) in differe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01586-15
更新日期:2015-10-01 00:00:00
abstract::Identification of cellular receptors used by coronavirus (CoV) entry into the host cells is critical to an understanding of pathogenesis and to development of intervention strategies. The fourth CoV genus, Deltacoronavirus, evolutionarily related to the Gammacoronavirus, has just been defined recently. In the current ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00318-18
更新日期:2018-05-29 00:00:00
abstract::Latency-associated nuclear antigen 1 (LANA1) of Kaposi's sarcoma-associated herpesvirus (KSHV) is implicated in the persistence of the viral genome during latent infection. It has been suggested that LANA1 tethers the viral genome to the host chromosome and also participates actively in DNA replication from the termin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7248-7256.2004
更新日期:2004-07-01 00:00:00
abstract::3-O-(3',3'-dimethylsuccinyl)betulinic acid (PA-457 or bevirimat) potently inhibits human immunodeficiency virus type 1 (HIV-1) maturation by blocking a late step in the Gag processing pathway, specifically the cleavage of SP1 from the C terminus of capsid (CA). To gain insights into the mechanism(s) by which HIV-1 cou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01369-06
更新日期:2006-11-01 00:00:00
abstract::The tsB5 mutant of herpes simplex virus type 1 (HSV-1) strain HFEM was shown previously to be temperature sensitive for accumulation of the mature form of glycoprotein gB, for production or activity of a factor required in virus-induced cell fusion, and for production of virions with normal levels of infectivity. In a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.114-128.1980
更新日期:1980-07-01 00:00:00
abstract::Previous analysis of hepatitis B virus (HBV) indicated base pairing between two cis-acting sequences, the 5' half of the upper stem of epsilon and phi, contributes to the synthesis of minus-strand DNA. Our goal was to identify other cis-acting sequences on the pregenomic RNA (pgRNA) involved in the synthesis of minus-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01414-07
更新日期:2007-11-01 00:00:00
abstract::The 3'-untranslated region (3'-UTR) of mouse hepatitis virus (MHV) RNA regulates the replication of and transcription from the viral RNA. Several host cell proteins have previously been shown to interact with this regulatory region. By immunoprecipitation of UV-cross-linked cellular proteins and in vitro binding of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5009-5017.2001
更新日期:2001-06-01 00:00:00
abstract::When residues 17 and 18 in nef of simian immunodeficiency virus strain SIVmac239 were changed from RQ to YE, the resultant virus was able to replicate in peripheral blood mononuclear cell cultures without prior lymphocyte activation and without the addition of exogenous interleukin-2, caused extensive lymphocyte activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.4157-4161.1996
更新日期:1996-06-01 00:00:00
abstract::The RNA genome of human immunodeficiency virus type 1 (HIV-1) is converted into DNA after infection in order to integrate into the host cell DNA. However, a large number of these reverse-transcribed genomes remain unintegrated in the nucleus of infected cells. Currently, there are no data available about the intranucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7683-7691.2001
更新日期:2001-08-01 00:00:00
abstract::Complement, part of the innate immune system, acts to remove pathogens and unwanted host material. Complement is known to function in all tissues, including the central nervous system (CNS). In this study, we demonstrated the importance of the complement system within the CNS in the development of behavioral seizures ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00422-10
更新日期:2010-07-01 00:00:00
abstract::We previously demonstrated that polyhedron formation (PF) mutants arise at a high frequency during serial passage of the Lymantria dispar nucleopolyhedrovirus (LdMNPV) in the L. dispar 652Y cell line (J. M. Slavicek, N. Hayes-Plazolles, and M. E. Kelly, Biol. Control 5:251-261, 1995). Most of these PF mutants exhibite...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1097-1106.1997
更新日期:1997-02-01 00:00:00
abstract::Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.608-618.1977
更新日期:1977-06-01 00:00:00
abstract::Human cytomegalovirus (HCMV) productive replication in vitro is most often studied in fibroblasts. In vivo, fibroblasts amplify viral titers, but transmission and pathogenesis require the infection of other cell types, most notably epithelial cells. In vitro, the study of HCMV infection of epithelial cells has been al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00903-18
更新日期:2018-07-31 00:00:00
abstract::Phage P22 can integrate as prophage into a recombination-deficient (Rec(-)) strain of Salmonella typhimurium. At 37 C, the integration efficiency is only 10% that in Rec(+) infection, but at 25 C the efficiencies in Rec(-) and Rec(+) hosts are similar. Rec(-) lysogens cannot be induced by ultraviolet irradiation or by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.7.702-709.1968
更新日期:1968-07-01 00:00:00
abstract::The protein product of varicella-zoster virus (VZV) ORF47 is a serine/threonine protein kinase and tegument component. Evaluation of two recombinants of the Oka strain, rOka47DeltaC, with a C-terminal truncation of ORF47, and rOka47D-N, with a point mutation in the conserved kinase motif, showed that ORF47 kinase func...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13293-13305.2004
更新日期:2004-12-01 00:00:00
abstract:UNLABELLED:The immunoglobulin superfamily protein receptors for poliovirus, human rhinovirus, and coxsackievirus B (CVB) serve to bind the viruses to target cells and to facilitate the release of the virus genome by catalyzing the transition from the mature infectious virus to the A-particle uncoating intermediate. Rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01790-14
更新日期:2014-10-01 00:00:00
abstract::Polyomavirus large T antigen has an N-terminal domain of approximately 260 amino acids which can immortalize primary cells but lacks sequences known to be required for DNA binding and replication. Treatment of full-length large T with either V8 protease or chymotrypsin yields an N-terminal fragment of 36 to 40 kDa and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.668-673.1994
更新日期:1994-02-01 00:00:00
abstract::Recombinant interleukin-16 (rIL-16) has been found to inhibit human immunodeficiency virus type 1 (HIV-1) replication in acutely or endogenously infected CD4(+) T cells. However, the effect of rIL-16 on HIV-1 replication in antigen-presenting cells (APCs) is still unknown. We show here a potent HIV-suppressive activit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.7008-7013.1999
更新日期:1999-08-01 00:00:00
abstract::Retroviruses are believed to induce tumors by acting as insertional mutagens that activate expression of cellular protooncogenes. Indeed, almost 90% of mouse mammary tumor virus (MMTV)-induced mammary tumors in C3H/He mice show upregulation of Int protooncogenes. We have analyzed three different MMTV variants [MMTV(C3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.8876-8883.2000
更新日期:2000-10-01 00:00:00
abstract::The characteristics of fusion of respiratory syncytial virus (RSV) with HEp-2 cells were studied by the R18 fluorescence dequenching assay of membrane fusion. A gradual increase in fluorescence intensity indicative of virion-cell fusion was observed when R18-labeled RSV was incubated with HEp-2 cells. Approximately 35...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4063-4069.1991
更新日期:1991-08-01 00:00:00
abstract::Several domains of CD4 have been suggested to play a critical role in events that follow its binding to the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (gp120-gp41). It has been reported previously that cells expressing a chimeric molecule consisting of the first 177 residues of human CD4 attache...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6469-6475.1993
更新日期:1993-11-01 00:00:00
abstract::Noninfectious spikeless particles have been obtained from vesicular stomatitis virus (VSV, Indiana serotype) by bromelain or Pronase treatment. They lack the viral glycoprotein (G) but contain all the other viral components (RNA, lipid, and other structural proteins). Triton-solubilized VSV-Indiana glycoprotein prepar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.75-84.1975
更新日期:1975-07-01 00:00:00
abstract::Cloned avian leukosis viral DNAs were mutagenized in the long terminal repeat, in the leader sequence for env mRNA, and at the poly-env junction. The effect of these mutations in the viral DNA upon its ability to direct virus production or env mRNA synthesis was analyzed by microinjecting the mutant DNAs into chicken ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.2.503-510.1982
更新日期:1982-08-01 00:00:00
abstract::The use of pathogen recognition receptor (PRR) agonists and the molecular mechanisms involved have been the major focus of research in individual vaccine development. West Nile virus (WNV) nonstructural (NS) 4B-P38G mutant has several features for an ideal vaccine candidate, including significantly reduced neuroinvasi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02097-16
更新日期:2017-02-28 00:00:00
abstract::Type I interferon is induced shortly following viral infection and represents a first line of host defense against a majority of viral pathogens. Not surprisingly, both replication and latency of gammaherpesviruses, ubiquitous cancer-associated pathogens, are attenuated by type I interferon, although the mechanism of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03278-13
更新日期:2014-02-01 00:00:00
abstract::Scrapie and Creutzfeldt-Jakob disease are transmissible, degenerative neurological diseases caused by prions. Considerable evidence argues that prions contain protease-resistant sialoglycoproteins, designated PrPSc, encoded by a cellular gene. The prion protein (PrP) gene also encodes a normal cellular protein designa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1558-1564.1988
更新日期:1988-05-01 00:00:00
abstract::Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is indispensable for viral DNA replication and episome maintenance in latency. Four promoters, Cp, Wp, Qp, and Fp, are known to drive EBNA1 expression. Here we show that the TATA-less Qp is constitutively active in a variety of EBV-positive [EBV(+)] tumors and cell li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7075-7083.1998
更新日期:1998-09-01 00:00:00
abstract::Superinfection of Corynebacterium diphtheriae C7(beta) by heteroimmune phage gamma is productive, whereas superinfection by gamma-bin mutants is for the most part nonproductive. Exclusion of gamma-bin phage occurred after its DNA had penetrated and was partially expressed in the heteroimmune lysogen. All of the infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.526-532.1980
更新日期:1980-11-01 00:00:00
abstract::Current human immunodeficiency virus type 1 (HIV-1) envelope vaccine candidates elicit high antibody binding titers with neutralizing activity against T-cell line-adapted but not primary HIV-1 isolates. Serum antibodies from these human vaccine recipients were also found to be preferentially directed to linear epitope...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4319-4330.1997
更新日期:1997-06-01 00:00:00