Abstract:
UNLABELLED:Phenotypic diversity in prion diseases can be specified by prion strains in which biological traits are propagated through an epigenetic mechanism mediated by distinct PrP(Sc) conformations. We investigated the role of host-dependent factors on phenotypic diversity of chronic wasting disease (CWD) in different host species that express the same prion protein gene (Prnp). Two CWD strains that have distinct biological, biochemical, and pathological features were identified in transgenic mice that express the Syrian golden hamster (SGH) Prnp. The CKY strain of CWD had a shorter incubation period than the WST strain of CWD, but after transmission to SGH, the incubation period of CKY CWD was ∼150 days longer than WST CWD. Limited proteinase K digestion revealed strain-specific PrP(Sc) polypeptide patterns that were maintained in both hosts, but the solubility and conformational stability of PrP(Sc) differed for the CWD strains in a host-dependent manner. WST CWD produced PrP(Sc) amyloid plaques in the brain of the SGH that were partially insoluble and stable at a high concentration of protein denaturant. However, in transgenic mice, PrP(Sc) from WST CWD did not assemble into plaques, was highly soluble, and had low conformational stability. Similar studies using the HY and DY strains of transmissible mink encephalopathy resulted in minor differences in prion biological and PrP(Sc) properties between transgenic mice and SGH. These findings indicate that host-specific pathways that are independent of Prnp can alter the PrP(Sc) conformation of certain prion strains, leading to changes in the biophysical properties of PrP(Sc), neuropathology, and clinical prion disease. IMPORTANCE:Prions are misfolded pathogenic proteins that cause neurodegeneration in humans and animals. Transmissible prion diseases exhibit a spectrum of disease phenotypes and the basis of this diversity is encoded in the structure of the pathogenic prion protein and propagated by an epigenetic mechanism. In the present study, we investigated prion diversity in two hosts species that express the same prion protein gene. While prior reports have demonstrated that prion strain properties are stable upon infection of the same host species and prion protein genotype, our findings indicate that certain prion strains can undergo dramatic changes in biological properties that are not dependent on the prion protein. Therefore, host factors independent of the prion protein can affect prion diversity. Understanding how host pathways can modify prion disease phenotypes may provide clues on how to alter prion formation and lead to treatments for prion, and other, human neurodegenerative diseases of protein misfolding.
journal_name
J Viroljournal_title
Journal of virologyauthors
Crowell J,Hughson A,Caughey B,Bessen RAdoi
10.1128/JVI.01586-15subject
Has Abstractpub_date
2015-10-01 00:00:00pages
10427-41issue
20eissn
0022-538Xissn
1098-5514pii
JVI.01586-15journal_volume
89pub_type
杂志文章abstract::Entry of the budded virus form of baculoviruses into insect and mammalian cells is generally thought to occur through a low-pH-dependent endocytosis pathway, possibly through clathrin-coated pits. This insight is primarily based on (immuno)electron microscopy studies but requires biochemical support to exclude the use...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00880-06
更新日期:2006-09-01 00:00:00
abstract::A marked virus-inhibiting potency is obtained in the serum after intraperitoneal injection of polyacrylic acid (PAA) and polymethacrylic acid (PMAA) in mice. Much higher antiviral levels were reached than for other related polymers including dextran sulfate, heparin, polyvinyl sulfate, pyran copolymer, polystyrene sul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.9.886-893.1968
更新日期:1968-09-01 00:00:00
abstract::The need to replace rabies immune globulin (RIG) as an essential component of rabies postexposure prophylaxis is widely acknowledged. We set out to discover a unique combination of human monoclonal antibodies (MAbs) able to replace RIG. Stringent criteria concerning neutralizing potency, affinity, breadth of neutraliz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.9062-9068.2005
更新日期:2005-07-01 00:00:00
abstract::Recombinant vaccinia viruses containing the 22-kilodalton protein (matrixlike or 22K protein) or phosphoprotein gene from respiratory syncytial virus were constructed. These recombinant viruses expressed proteins which were immunoprecipitated by appropriate respiratory syncytial virus antibodies and comigrated with au...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.4.1683-1689.1990
更新日期:1990-04-01 00:00:00
abstract::Human cytomegalovirus (HCMV) infection of monocyte-derived macrophages (MDM) results in delayed and nonlytic productive viral growth. During late stages of replication, infectious virus remains cell associated in cytoplasmic vacuoles. In order to understand HCMV survival and persistence in MDM, we examined mechanisms ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1855-1862.1996
更新日期:1996-03-01 00:00:00
abstract::We analyzed capsomeres of human papillomavirus type 16 (HPV16) consisting of the L1 major structural protein for their ability to trigger a cytotoxic T-cell (CTL) response. To this end, we immunized C57BL/6 mice and used the L1(165-173) peptide for ex vivo restimulation of splenocytes prior to analysis ((51)Cr release...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4635-4645.2003
更新日期:2003-04-01 00:00:00
abstract::A soluble polymerase-template complex prepared from poliovirus-infected cells was found to incorporate radioactive UTP into trichloroacetic acid-insoluble RNA linearly for 8 h in the presence of ATP and Mg2+. Radioactive CTP or GTP was not incorporated under identical conditions. Nearest-neighbor analysis of the in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.61-68.1977
更新日期:1977-01-01 00:00:00
abstract::The family Geminiviridae comprises seven genera differentiated by genome organization, sequence similarity, and insect vector. Capulavirus, an eighth genus, has been proposed to accommodate two newly discovered highly divergent geminiviruses that presently have no known vector. Alfalfa leaf curl virus, identified here...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00453-15
更新日期:2015-09-01 00:00:00
abstract::The carboxyl-terminal three-fourths of the hepatitis C virus (HCV) NS3 protein has been shown to possess an RNA helicase activity, typical of members of the DEAD box family of RNA helicases. In addition, the NS3 protein contains four amino acid motifs conserved in DEAD box proteins. In order to inspect the roles of in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9400-9409.1997
更新日期:1997-12-01 00:00:00
abstract::Hepatitis delta virus (HDV) is a defective RNA virus which is dependent on hepatitis B virus for essential helper functions. Only a single highly basic phosphoprotein, HDV antigen (HDAg), is expressed by the HDV genome during infection in humans. Antibody directed to HDAg is important in the diagnosis of HDV infection...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1108-1116.1990
更新日期:1990-03-01 00:00:00
abstract::Nine pre-early polypeptides have been detected after infection with bacteriophage T5 and 10 pre-early polypeptides have been detected after infection with bacteriophage BF23. Only about one-half of the coding capacity of the redundant ends of the phage DNA, which code for pre-early proteins, is needed for these 9 to 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.2.480-488.1977
更新日期:1977-05-01 00:00:00
abstract::We analyzed xenotropic murine leukemia virus-related virus (XMRV) integration site sequences previously identified from human prostate tissues for single-nucleotide polymorphisms (SNPs) to discriminate between patient and potential cell line sources of the proviruses. The SNPs of two integration sites were identical t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05624-11
更新日期:2011-12-01 00:00:00
abstract::The small envelope protein (E) plays a role of central importance in the assembly of coronaviruses. This was initially established by studies demonstrating that cellular expression of only E protein and the membrane protein (M) was necessary and sufficient for the generation and release of virus-like particles. To inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01577-06
更新日期:2007-03-01 00:00:00
abstract::Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after treatment, antigenic markers and DNA polymerase activities specific to particles of each of the two types were assayed in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.709-716.1976
更新日期:1976-08-01 00:00:00
abstract::Marek's disease virus (MDV) is an alphaherpesvirus for which infection is strictly cell associated in permissive cell culture systems. In contrast to most other alphaherpesviruses, no comprehensive ultrastructural study has been published to date describing the different stages of MDV morphogenesis. To circumvent prob...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01177-07
更新日期:2007-11-01 00:00:00
abstract::The abilities to infect and transmit efficiently among humans are essential for a novel influenza A virus to cause a pandemic. To evaluate the pandemic potential of widely disseminated H5N1 influenza viruses, a ferret contact model using experimental groups comprised of one inoculated ferret and two contact ferrets wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00170-07
更新日期:2007-07-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of PRRS, which has important impacts on the pig industry. PRRSV infection results in disruption of the swine leukocyte antigen class I (SLA-I) antigen presentation pathway. In this study, highly pathogenic PRRSV (HP-PRRSV) infection inh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01755-16
更新日期:2017-02-14 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is the most important cause of acute respiratory disease in infants. Two major subgroups (A and B) have been identified based on antigenic differences in the attachment G protein. Antigenic variation between and within the subgroups may contribute to reinfections with these vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.9157-9167.2005
更新日期:2005-07-01 00:00:00
abstract::Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I . J fragment (the left 7.8% of the viral genome) and in human KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.337-352.1980
更新日期:1980-11-01 00:00:00
abstract::The relative antigenicity of the individual herpes simplex virus type 1 (KOS) glycoproteins gC and gB was analyzed in BALB/c mice by using KOS mutants altered in their ability to present these antigens on cell surface membranes during infection. The mutants employed were as follows: syn LD70 , a non-temperature-sensit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.805-812.1984
更新日期:1984-06-01 00:00:00
abstract::The L1 and L2 capsid genes of human papillomavirus type 31 (HPV-31) are expressed upon keratinocyte differentiation from a promoter located in the E7 open reading frame (ORF) of the early region. Late transcripts must therefore pass through and ignore the early polyadenylation sequences to use the downstream late AAUA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.17.8147-8157.2001
更新日期:2001-09-01 00:00:00
abstract::Replication of flock house virus (FHV) RNA1 and production of subgenomic RNA3 in the yeast Saccharomyces cerevisiae provide a useful tool for the dissection of FHV molecular biology and host-encoded functions involved in RNA replication. The replication template activity of RNA1 can be separated from its coding potent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.3905-3919.2002
更新日期:2002-04-01 00:00:00
abstract::Extracts prepared from either mouse cells or monkey cells were examined for the ability to support in vitro bovine papillomavirus type 1 (BPV1) DNA replication, and they were used in parallel as a source of host replication proteins for affinity chromatography. DNA synthesis exhibited an absolute requirement for BPV1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2341-2350.1995
更新日期:1995-04-01 00:00:00
abstract::DNA sequences encoding the surface envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) were amplified by PCR from uncultured peripheral blood mononuclear cells obtained from patients with serologically defined HIV-1/HIV-2 mixed infections from Bombay, India. HIV-1-specific PCR prod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2161-2168.1994
更新日期:1994-04-01 00:00:00
abstract:UNLABELLED:Crimean-Congo hemorrhagic fever virus (CCHFV; genus Nairovirus) is an extremely pathogenic member of the Bunyaviridae family. Since handling of the virus requires a biosafety level 4 (BSL-4) facility, little is known about pathomechanisms and host interactions. Here, we describe the establishment of a transc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03691-14
更新日期:2015-06-01 00:00:00
abstract::A potentially powerful approach for in vivo gene delivery is to target retrovirus to specific cells through interactions between cell surface receptors and appropriately modified viral envelope proteins. Previously, relatively large (>100 residues) protein ligands to cell surface receptors have been inserted at or nea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3558-3563.2002
更新日期:2002-04-01 00:00:00
abstract:UNLABELLED:Enterovirus 71 (EV71) recruits various cellular factors to assist in the replication and translation of its genome. Identification of the host factors involved in the EV71 life cycle not only will enable a better understanding of the infection mechanism but also has the potential to be of use in the developm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01677-15
更新日期:2015-10-01 00:00:00
abstract::The prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a formidable battle horse for the study of viral immunology, as well as viral persistence and associated diseases. Investigations with LCMV have uncovered basic mechanisms by which viruses avoid elimination by the host adaptive immune response. In ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00555-06
更新日期:2006-09-01 00:00:00
abstract::The vpr gene product of human immunodeficiency virus type (HIV-1) is a virion-associated regulatory protein. A transferable virion association motif for Vpr is located in the p6 domain of the HIV-1 Gag polyprotein. To map the sequences in p6 that are involved in Vpr incorporation, we analyzed the ability of mutant for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.159-164.1996
更新日期:1996-01-01 00:00:00
abstract::Production of human papillomavirus type 16 (HPV-16) virus particles is totally dependent on the differentiation-dependent induction of viral L1 and L2 late gene expression. The early polyadenylation signal in HPV-16 plays a major role in the switch from the early to the late, productive stage of the viral life cycle. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.9254-9269.2005
更新日期:2005-07-01 00:00:00