Abstract:
:The carboxyl-terminal three-fourths of the hepatitis C virus (HCV) NS3 protein has been shown to possess an RNA helicase activity, typical of members of the DEAD box family of RNA helicases. In addition, the NS3 protein contains four amino acid motifs conserved in DEAD box proteins. In order to inspect the roles of individual amino acid residues in the four conserved motifs (AXXXXGKS, DECH, TAT, and QRRGRTGR) of the NS3 protein, mutational analysis was used in this study. Thirteen mutant proteins were constructed, and their biochemical activities were examined. Lys1235 in the AXXXXGKS motif was important for basal nucleoside triphosphatase (NTPase) activity in the absence of polynucleotide cofactor. A serine in the X position of the DEXH motif disrupted the NTPase and RNA helicase activities. Alanine substitution at His1318 of the DEXH motif made the protein possess high NTPase activity. In addition, we now report inhibition of NTPase activity of NS3 by polynucleotide cofactor. Gln1486 was indispensable for the enzyme activity, and this residue represents a distinguishing feature between DEAD box and DEXH proteins. There are four Arg residues in the QRRGRTGR motif of the HCV NS3 protein, and the second, Arg1488, was important for RNA binding and enzyme activity, even though it is less well conserved than other Arg residues. Arg1490 and Arg1493 were essential for the enzymatic activity. As the various enzymatic activities were altered by mutation, the enzyme characteristics were also changed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kim DW,Kim J,Gwack Y,Han JH,Choe Jdoi
10.1128/JVI.71.12.9400-9409.1997subject
Has Abstractpub_date
1997-12-01 00:00:00pages
9400-9issue
12eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2332-2338.1996
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abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) (also called human herpesvirus 8) is consistently found in Kaposi's sarcoma lesions and in body-cavity-based lymphomas. A 17-kb KSHV lambda clone was obtained directly from a Kaposi's sarcoma lesion. DNA sequence analysis of this clone identified an open reading frame whi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1984-1991.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8360-8373.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3190-3198.1987
更新日期:1987-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.78.15.7874-7882.2004
更新日期:2004-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.877-881.1982
更新日期:1982-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.02723-07
更新日期:2008-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3930-3937.1996
更新日期:1996-06-01 00:00:00
abstract::We have determined the complete nucleotide sequence of an infectious cloned genome of ground squirrel hepatitis virus (GSHV), a nonpathogenic member of the hepadnavirus group. The genome is 3,311 base pairs long and contains the major open reading frames described for the related human and woodchuck hepatitis B viruse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.2.367-375.1984
更新日期:1984-08-01 00:00:00
abstract::The human cytomegalovirus (HCMV) UL138 protein downregulates the cell surface expression of the multidrug resistance-associated protein 1 (MRP1) transporter. We examined the genetic requirements within UL138 for MRP1 downregulation. We determined that the acidic cluster dileucine motif is essential for UL138-mediated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00430-19
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00872-19
更新日期:2019-11-26 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.1.142-153.1980
更新日期:1980-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.9.886-893.1968
更新日期:1968-09-01 00:00:00
abstract::Clinical deterioration in human immunodeficiency virus type 1 (HIV-1) disease is associated with an increased viral burden in the peripheral blood and a loss of circulating CD4+ T cells. HIV-1 isolates obtained prior to this stage of disease often have a "slow-low," non-syncytium-inducing (NSI) phenotype, whereas thos...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.8188-8192.1994
更新日期:1994-12-01 00:00:00
abstract::The protein product of varicella-zoster virus (VZV) ORF47 is a serine/threonine protein kinase and tegument component. Evaluation of two recombinants of the Oka strain, rOka47DeltaC, with a C-terminal truncation of ORF47, and rOka47D-N, with a point mutation in the conserved kinase motif, showed that ORF47 kinase func...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10100-10107.1998
更新日期:1998-12-01 00:00:00
abstract::We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and O. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyces cerevisiae plasmids ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.5157-5160.1992
更新日期:1992-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02170-14
更新日期:2015-01-01 00:00:00
abstract::Intracellular mature vaccinia virions are wrapped by cisternae, derived from virus-modified trans-Golgi or endosomal membranes, and then transported via microtubules to the cell periphery. Two viral proteins, encoded by the F13L and B5R open reading frames, are essential for the membrane-wrapping step. Previous transf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7777-7789.2002
更新日期:2002-08-01 00:00:00
abstract::Several viral determinants were shown to play a role in the ability of human immunodeficiency virus type 1 (HIV-1) to infect nondividing cells. In particular, Vpr and Gag matrix (MA) were recognized to be involved in the nuclear transport of the viral preintegration complex. The goal of the present study was to evalua...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4104-4115.1998
更新日期:1998-05-01 00:00:00
abstract::Myeloid differentiation factor 88 (MyD88) is an essential adaptor protein in the Toll-like receptor-mediated innate signaling pathway, as well as in interleukin-1 receptor (IL-1R) and IL-18R signaling. The importance of MyD88 in the regulation of innate immunity to microbial pathogens has been well demonstrated. Howev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01770-08
更新日期:2009-02-01 00:00:00
abstract::Incubation of highly purified adeno-associated virus (AAV) capsids in vitro at pH 5.5 induced significant autocleavage of capsid proteins at several amino acid positions. No autocleavage was seen at pH 7.5. Examination of other AAV serotypes showed at least two different pH-induced cleavage patterns, suggesting that d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01717-12
更新日期:2012-11-01 00:00:00
abstract::The highly syncytium-inducing M strain and the weakly syncytium-inducing SC strain of bovine parainfluenza 3 virus differ by a single amino acid substitution in each of the hemagglutinin-neuraminidase (HN) and membrane (M) proteins, while their fusion (F) proteins are identical (T. Shioda, S. Wakao, S. Suzu, and H. Sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3661-3668.1989
更新日期:1989-09-01 00:00:00
abstract::All plus-strand RNA viruses encode an RNA-dependent RNA polymerase (RdRp) that functions as the catalytic subunit of the viral replication/transcription complex, directing viral RNA synthesis in concert with other viral proteins and, sometimes, host proteins. RNA synthesis essentially can be initiated by two different...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00564-07
更新日期:2007-08-01 00:00:00
abstract::Equine infectious anemia virus was found to be comprised of fourteen polypeptides of molecular weight ranging from 10,000 to 79,000. Eighty percent of the virion protein was accounted for by five polypeptides, including two non-glycosylated components (p29 and p13) comprising one-half of the virion protein and three g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.997-1001.1978
更新日期:1978-12-01 00:00:00
abstract::Changes in the envelope glycoprotein ectodomains of a nonpathogenic simian-human immunodeficiency virus (SHIV-89.6) that was serially passaged in vivo have been shown to be responsible for the increased pathogenicity of the resulting virus, SHIV-KB9 (G. B. Karlsson, et al., J. Exp. Med. 188:1159-1171, 1998). The 12 am...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4433-4440.2000
更新日期:2000-05-01 00:00:00