Abstract:
:Multispecific CD8(+) T-cell responses are thought to be important for the control of acute hepatitis C virus (HCV) infection, but to date little information is actually available on the breadth of responses at early time points. Additionally, the influence of early therapy on these responses and their relationships to outcome are controversial. To investigate this issue, we performed comprehensive analysis of the breadth and frequencies of virus-specific CD8(+) T-cell responses on the single epitope level in eight acutely infected individuals who were all started on early therapy. During the acute phase, responses against up to five peptides were identified. During therapy, CD8(+) T-cell responses decreased rather than increased as virus was controlled, and no new specificities emerged. A sustained virological response following completion of treatment was independent of CD8(+) T-cell responses, as well as CD4(+) T-cell responses. Rapid recrudescence also occurred despite broad CD8(+) T-cell responses. Importantly, in vivo suppression of CD3(+) T cells using OKT3 in one subject did not result in recurrence of viremia. These data suggest that broad CD8(+) T-cell responses alone may be insufficient to contain HCV replication, and also that early therapy is effective independent of such responses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lauer GM,Lucas M,Timm J,Ouchi K,Kim AY,Day CL,Schulze Zur Wiesch J,Paranhos-Baccala G,Sheridan I,Casson DR,Reiser M,Gandhi RT,Li B,Allen TM,Chung RT,Klenerman P,Walker BDdoi
10.1128/JVI.79.20.12979-12988.2005subject
Has Abstractpub_date
2005-10-01 00:00:00pages
12979-88issue
20eissn
0022-538Xissn
1098-5514pii
79/20/12979journal_volume
79pub_type
杂志文章abstract::Activation of microglia and astroglia is seen in many neurodegenerative diseases including prion diseases. Activated glial cells produce cytokines as a protective response against certain pathogens and as part of the host inflammatory response to brain damage. In addition, cytokines might also exacerbate tissue damage...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01413-09
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abstract::From the results of transient plasmid-based replication assays, it has been postulated that homologous regions (hrs) of Autographa californica nuclear polyhedrosis virus (AcMNPV) function as origins of viral DNA replication. However, these assays vary in specificity according to the methodology used and may not be dep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6967-6972.1996
更新日期:1996-10-01 00:00:00
abstract::The interaction of papillomavirus E2 proteins with cellular Brd4 protein is important for transcriptional regulation of viral genes and partitioning of viral genomes. Bovine papillomavirus type 1 (BPV-1) E2 binds cellular chromatin in complex with Brd4 in both mitotic and interphase cells. To identify specific sites o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02275-08
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abstract::Latent membrane protein 2A (LMP2A) is one of only two viral proteins expressed during latent Epstein-Barr virus (EBV) infections in human peripheral B cells. LMP2A blocks B-cell receptor (BCR) signal transduction in vitro by modulation of the Syk and Lyn protein tyrosine kinases. Five genetically unique LMP2A transgen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.3.1101-1113.2000
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abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) nsp1 protein has unique biological functions that have not been described in the viral proteins of any RNA viruses; expressed SARS-CoV nsp1 protein has been found to suppress host gene expression by promoting host mRNA degradation and inhibiting translation....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02472-07
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abstract::Previous results have indicated that Rous sarcoma virus env gene expression is specifically inhibited by antisense RNA (L.-J. Chang and C. M. Stoltzfus, Mol. Cell. Biol. 5:2341-2348, 1985). In this study, we compare the extents of inhibition by antisense RNA derived from different parts of the Rous sarcoma virus genom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.3.921-924.1987
更新日期:1987-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8665-8668.2005
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abstract::A recombinant capripoxvirus vaccine containing a cDNA of the peste-des-petits-ruminants virus (PPRV) fusion protein gene was constructed. A quick and efficient method was used to select a highly purified recombinant virus clone. A trial showed that a dose of this recombinant as low as 0.1 PFU protected goats against c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1571-1577.2003
更新日期:2003-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1447-1452.1999
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00938-18
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abstract::We have previously reported that infection with herpes simplex virus type 1 (HSV-1) activates expression of the human immunodeficiency virus type 1 (HIV-1) provirus in T cells. Activation of the HIV-1 provirus correlated with the activation of binding of 55- and 85-kDa proteins to the kappa B enhancer and binding of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4427-4431.1993
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abstract::The chimeric recombinant virus rHPIV3-N(B), a version of human parainfluenza virus type 3 (HPIV3) that is attenuated due to the presence of the bovine PIV3 nucleocapsid (N) protein open reading frame (ORF) in place of the HPIV3 ORF, was modified to encode the measles virus hemagglutinin (HA) inserted as an additional,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10498-10504.2001
更新日期:2001-11-01 00:00:00
abstract::The contribution of the hepatitis B virus enhancers I and II in the regulation of the activity of the core and the X promoters was assessed in transgenic mice. Surprisingly, despite the presence of heterologous promoters linked 5' of the X gene, the transgene expression is mostly due to core promoter (Cp) activity pre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5912-5916.1995
更新日期:1995-09-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV/human herpesvirus 8 [HHV-8]) is a gamma-2-herpesvirus responsible for Kaposi's sarcoma as well as primary effusion lymphoma (PEL). KSHV is a lymphotropic virus that has pirated many mammalian genes involved in inflammation, cell cycle control, and angiogenesis. Among these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.57-67.2003
更新日期:2003-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.893-898.1986
更新日期:1986-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01177-07
更新日期:2007-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3108-3117.1996
更新日期:1996-05-01 00:00:00
abstract::Cytomegalovirus (CMV) disease after bone marrow (BM) transplantation is often associated with BM graft failure. There are two possible reasons for such a correlation. First, a poor hematopoietic reconstitution of unrelated etiology could promote the progression of CMV infection by the lack of immune control. Alternati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.5006-5015.1998
更新日期:1998-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01115-12
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01945-14
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.6182-6187.1999
更新日期:1999-07-01 00:00:00
abstract::Hepatitis delta virus (HDV) contains a circular RNA genome of 1.7 kb. HDV RNA replication is thought to proceed via a rolling-circle mechanism that is dependent on autocatalytic cleavage and ligation reactions. However, it has never been established that these ribozyme activities are indeed involved in HDV RNA replica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2228-2234.1993
更新日期:1993-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9507-9514.2000
更新日期:2000-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1092-1098.1999
更新日期:1999-02-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) clade C causes >50% of all HIV infections worldwide, and an estimated 90% of all transmissions occur mucosally with R5 strains. A pathogenic R5 simian-human immunodeficiency virus (SHIV) encoding HIV clade C env is highly desirable to evaluate candidate AIDS vaccines in nonh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00558-06
更新日期:2006-09-01 00:00:00
abstract:UNLABELLED:Virus entry into cells is a multistep process that often requires the subversion of subcellular machineries. A more complete understanding of these steps is necessary to develop new antiviral strategies. While studying the potential role of the actin network and one of its master regulators, the small GTPase...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00103-16
更新日期:2016-04-14 00:00:00
abstract::Geminiviruses package circular single-stranded DNA and replicate in the nucleus via a double-stranded intermediate. This intermediate also serves as a template for bidirectional transcription by polymerase II. Here, we map promoters and transcripts and characterize regulatory proteins of Mungbean yellow mosaic virus-V...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8149-8163.2005
更新日期:2005-07-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a lymphotrophic herpesvirus infecting most of the world's population. It is associated with a number of human lymphoid and epithelial tumors and lymphoproliferative diseases in immunocompromised patients. Recent studies have shown an in vitro and in vivo interaction between the EBV nuclear ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.17.8702-8709.2002
更新日期:2002-09-01 00:00:00
abstract::Herpes simplex virus DNA replication proteins localize in characteristic patterns corresponding to viral DNA replication structures in the infected cell nucleus. The intranuclear spatial organization of the HSV DNA replication structures and the factors regulating their nuclear location remain to be defined. We have u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3512-3526.1994
更新日期:1994-06-01 00:00:00