Abstract:
:The chimeric recombinant virus rHPIV3-N(B), a version of human parainfluenza virus type 3 (HPIV3) that is attenuated due to the presence of the bovine PIV3 nucleocapsid (N) protein open reading frame (ORF) in place of the HPIV3 ORF, was modified to encode the measles virus hemagglutinin (HA) inserted as an additional, supernumerary gene between the HPIV3 P and M genes. This recombinant, designated rHPIV3-N(B)HA, replicated like its attenuated rHPIV3-N(B) parent virus in vitro and in the upper and lower respiratory tracts of rhesus monkeys, indicating that the insertion of the measles virus HA did not further attenuate rHPIV3-N(B) in vitro or in vivo. Monkeys immunized with rHPIV3-N(B)HA developed a vigorous immune response to both measles virus and HPIV3, with serum antibody titers to both measles virus (neutralizing antibody) and HPIV3 (hemagglutination inhibiting antibody) of over 1:500. An attenuated HPIV3 expressing a major protective antigen of measles virus provides a method for immunization against measles by the intranasal route, a route that has been shown with HPIV3 and respiratory syncytial virus vaccines to be relatively refractory to the neutralizing and immunosuppressive effects of maternally derived virus-specific serum antibodies. It should now be possible to induce a protective immune response against measles virus in 6-month-old infants, an age group that in developing areas of the world is not responsive to the current measles virus vaccine.
journal_name
J Viroljournal_title
Journal of virologyauthors
Skiadopoulos MH,Surman SR,Riggs JM,Collins PL,Murphy BRdoi
10.1128/JVI.75.21.10498-10504.2001subject
Has Abstractpub_date
2001-11-01 00:00:00pages
10498-504issue
21eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
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journal_title:Journal of virology
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更新日期:1969-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1982-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6067-6070.1996
更新日期:1996-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8098-8104.1998
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journal_title:Journal of virology
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更新日期:1993-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 历史文章,杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5224-5231.1992
更新日期:1992-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
abstract::Adeno-associated virus type 2 (AAV-2) gene expression is tightly controlled by functions of the helper virus as well as by the products of its own viral rep gene. Double-immunofluorescence studies of Rep and VP protein expression in cells coinfected with AAV-2 and adenovirus type 2 showed that a large proportion of th...
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pub_type: 杂志文章
doi:10.1128/JVI.71.11.8437-8447.1997
更新日期:1997-11-01 00:00:00